WO2002061143A3 - Comparative analysis of nucleic acids using population tagging - Google Patents

Comparative analysis of nucleic acids using population tagging Download PDF

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Publication number
WO2002061143A3
WO2002061143A3 PCT/US2002/003097 US0203097W WO02061143A3 WO 2002061143 A3 WO2002061143 A3 WO 2002061143A3 US 0203097 W US0203097 W US 0203097W WO 02061143 A3 WO02061143 A3 WO 02061143A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
samples
comparative analysis
nucleic acid
sample
Prior art date
Application number
PCT/US2002/003097
Other languages
French (fr)
Other versions
WO2002061143A2 (en
Inventor
Matthew M Winkler
David Brown
Original Assignee
Ambion Inc
Matthew M Winkler
David Brown
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc, Matthew M Winkler, David Brown filed Critical Ambion Inc
Priority to EP02720894A priority Critical patent/EP1360333A2/en
Publication of WO2002061143A2 publication Critical patent/WO2002061143A2/en
Publication of WO2002061143A3 publication Critical patent/WO2002061143A3/en
Priority to US10/632,534 priority patent/US20040058373A1/en
Priority to US10/632,539 priority patent/US20040110191A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Abstract

Disclosed are methods that allow one or more nucleic acid targets to be compared across two or more nucleic acid samples. Nucleic acid tags are appended to the samples to be assessed, such that each sample has a unique tag. The tagged nucleic acids are then mixed, and the targets within the mixture are amplified. The amplification products are distinguished using the unique tag domains to reveal the abundance of the amplification products derived from each sample, which correlates to the relative abundance of the target in the samples.
PCT/US2002/003097 2001-01-31 2002-01-31 Comparative analysis of nucleic acids using population tagging WO2002061143A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02720894A EP1360333A2 (en) 2001-01-31 2002-01-31 Comparative analysis of nucleic acids using population tagging
US10/632,534 US20040058373A1 (en) 2001-01-31 2003-07-31 Competitive amplification of fractionated targets from multiple nucleic acid samples
US10/632,539 US20040110191A1 (en) 2001-01-31 2003-07-31 Comparative analysis of nucleic acids using population tagging

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26569401P 2001-01-31 2001-01-31
US60/265,694 2001-01-31

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/632,539 Continuation US20040110191A1 (en) 2001-01-31 2003-07-31 Comparative analysis of nucleic acids using population tagging

Publications (2)

Publication Number Publication Date
WO2002061143A2 WO2002061143A2 (en) 2002-08-08
WO2002061143A3 true WO2002061143A3 (en) 2003-03-27

Family

ID=23011507

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003097 WO2002061143A2 (en) 2001-01-31 2002-01-31 Comparative analysis of nucleic acids using population tagging

Country Status (2)

Country Link
EP (1) EP1360333A2 (en)
WO (1) WO2002061143A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017186A2 (en) 2003-08-08 2005-02-24 Thomas Jefferson University Method for rapid identification of alternative splicing
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
DE602005025782D1 (en) 2004-09-21 2011-02-17 Life Technologies Corp TWO-TONE REAL-TIME / END POINT QUANTIFICATION OF MICRO-RNAS (MIRNAS)
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
AU2006341607B2 (en) * 2005-05-31 2011-03-17 Applied Biosystems, Llc. Multiplexed amplification of short nucleic acids
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2589668A1 (en) * 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
DK2518162T3 (en) * 2006-11-15 2018-06-18 Biospherex Llc Multi-tag sequencing and ecogenomic analysis
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
WO2013022961A1 (en) * 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416817A2 (en) * 1989-09-06 1991-03-13 Zeneca Limited Amplification processes
WO1998008973A1 (en) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Global amplification of nucleic acids
EP0870842A2 (en) * 1997-04-07 1998-10-14 Japan Science and Technology Corporation Adaptor-tagged competitive PCR

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416817A2 (en) * 1989-09-06 1991-03-13 Zeneca Limited Amplification processes
WO1998008973A1 (en) * 1996-08-29 1998-03-05 Cancer Research Campaign Technology Limited Global amplification of nucleic acids
EP0870842A2 (en) * 1997-04-07 1998-10-14 Japan Science and Technology Corporation Adaptor-tagged competitive PCR

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US9441267B2 (en) 1999-04-20 2016-09-13 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

Also Published As

Publication number Publication date
WO2002061143A2 (en) 2002-08-08
EP1360333A2 (en) 2003-11-12

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