WO2002056024A3 - Detection methods - Google Patents

Detection methods Download PDF

Info

Publication number
WO2002056024A3
WO2002056024A3 PCT/GB2002/000127 GB0200127W WO02056024A3 WO 2002056024 A3 WO2002056024 A3 WO 2002056024A3 GB 0200127 W GB0200127 W GB 0200127W WO 02056024 A3 WO02056024 A3 WO 02056024A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
target molecule
reporter groups
detector protein
binding
Prior art date
Application number
PCT/GB2002/000127
Other languages
French (fr)
Other versions
WO2002056024A2 (en
Inventor
Amelie KARLSTRÖM
Per-Åke Nygren
Original Assignee
Affibody Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0100919A external-priority patent/GB0100919D0/en
Application filed by Affibody Ab filed Critical Affibody Ab
Priority to CA002434418A priority Critical patent/CA2434418A1/en
Priority to AU2002225136A priority patent/AU2002225136A1/en
Priority to US10/250,927 priority patent/US20040077017A1/en
Priority to JP2002556228A priority patent/JP2005504261A/en
Priority to EP02715487A priority patent/EP1352242A2/en
Publication of WO2002056024A2 publication Critical patent/WO2002056024A2/en
Publication of WO2002056024A3 publication Critical patent/WO2002056024A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
    • G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)

Abstract

The present invention relates to methods of detecting the presence of target molecules in a sample, in particular protein target molecules. In particular, the invention provides a method of detecting the presence of a target molecule in a sample which comprises contacting said sample with a detector protein, said detector protein being a binding partner for said target molecule and being derivatized by two fluorescent reporter groups, the energy transfer between said reporter groups undergoing a detectable change on binding of the target molecule to said detector protein and a detector protein molecule having a binding site for a target molecule and having covalently attached thereto two fluorescent reporter groups, the energy transfer between said reporter groups undergoing a detectable change on binding of the target molecule to said detector protein. The detector protein comprises or consists of a combinatorial protein.
PCT/GB2002/000127 2001-01-12 2002-01-14 Detection methods WO2002056024A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002434418A CA2434418A1 (en) 2001-01-12 2002-01-14 Detection methods
AU2002225136A AU2002225136A1 (en) 2001-01-12 2002-01-14 Detection methods
US10/250,927 US20040077017A1 (en) 2001-01-12 2002-01-14 Detection methods
JP2002556228A JP2005504261A (en) 2001-01-12 2002-01-14 Detection method
EP02715487A EP1352242A2 (en) 2001-01-12 2002-01-14 Detection methods

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0100919.0 2001-01-12
GB0100919A GB0100919D0 (en) 2001-01-12 2001-01-12 Detection methods
US28959501P 2001-05-08 2001-05-08
US60/289,595 2001-05-08
CA 2346583 CA2346583A1 (en) 2001-01-12 2001-05-08 Detection methods
CA2,346,583 2001-05-08

Publications (2)

Publication Number Publication Date
WO2002056024A2 WO2002056024A2 (en) 2002-07-18
WO2002056024A3 true WO2002056024A3 (en) 2003-03-06

Family

ID=27171533

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000127 WO2002056024A2 (en) 2001-01-12 2002-01-14 Detection methods

Country Status (3)

Country Link
EP (1) EP1352242A2 (en)
JP (1) JP2005504261A (en)
WO (1) WO2002056024A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2545006C (en) * 2003-12-12 2013-09-17 Saint Louis University Biosensors for detecting macromolecules and other analytes
WO2006083932A2 (en) * 2005-01-31 2006-08-10 The Regents Of The University Methods and compositions for aggregant detection
JP2022501444A (en) * 2018-09-27 2022-01-06 ナヴロジェン インコーポレイテッド Compositions and Methods for Measuring Antibody Dynamics

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777128A (en) * 1986-05-27 1988-10-11 Ethigen Corporation Fluorescence immunoassay involving energy transfer between two fluorophores
US5831012A (en) * 1994-01-14 1998-11-03 Pharmacia & Upjohn Aktiebolag Bacterial receptor structures
WO2000008444A1 (en) * 1998-08-08 2000-02-17 Imperial Cancer Research Technology Limited Fluorescent assay for biological systems
WO2000063243A1 (en) * 1999-04-19 2000-10-26 Biovitrum Ab Derivatives of the b or z domain from staphylococcal protein a (spa) interacting with at least one domain of human factor viii

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777128A (en) * 1986-05-27 1988-10-11 Ethigen Corporation Fluorescence immunoassay involving energy transfer between two fluorophores
US5831012A (en) * 1994-01-14 1998-11-03 Pharmacia & Upjohn Aktiebolag Bacterial receptor structures
WO2000008444A1 (en) * 1998-08-08 2000-02-17 Imperial Cancer Research Technology Limited Fluorescent assay for biological systems
WO2000063243A1 (en) * 1999-04-19 2000-10-26 Biovitrum Ab Derivatives of the b or z domain from staphylococcal protein a (spa) interacting with at least one domain of human factor viii

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DOI N ET AL: "Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 453, no. 3, 25 June 1999 (1999-06-25), pages 305 - 307, XP004259893, ISSN: 0014-5793 *
FISHER C A ET AL: "LIPID BINDING-INDUCED CONFORMATIONAL CHANGES IN THE N-TERMINAL DOMAIN OF HUMAN APOLIPOPROTEIN E", JOURNAL OF LIPID RESEARCH, BETHESDA, MD, US, vol. 40, no. 1, January 1999 (1999-01-01), pages 93 - 99, XP000989977, ISSN: 0022-2275 *
GODWIN H A ET AL: "A FLUORESCENT ZINC PROBE NASED ON METAL-INDUCED PEPTIDE FOLDING", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 118, no. 27, 1996, UNITED STATES, pages 6514 - 6515, XP002224549 *
KARLSTROM AMELIE ET AL: "Dual labeling of a binding protein allows for specific fluorescence detection of native protein.", ANALYTICAL BIOCHEMISTRY, vol. 295, no. 1, 1 August 2001 (2001-08-01), pages 22 - 30, XP002224547, ISSN: 0003-2697 *
SINEV MICHAEL ET AL: "Design consideration and probes for fluorescence resonance energy transfer studies.", BIOCONJUGATE CHEMISTRY, vol. 11, no. 3, May 2000 (2000-05-01), pages 352 - 362, XP002224548, ISSN: 1043-1802 *
ZHAO AND F H ARNOLD H: "Combinatorial protein design: strategies for screening protein libraries", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 7, 1997, pages 480 - 485, XP002133873, ISSN: 0959-440X *

Also Published As

Publication number Publication date
WO2002056024A2 (en) 2002-07-18
EP1352242A2 (en) 2003-10-15
JP2005504261A (en) 2005-02-10

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