WO2002052031A3 - Nucleic acid amplification - Google Patents

Nucleic acid amplification Download PDF

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Publication number
WO2002052031A3
WO2002052031A3 PCT/US2001/050340 US0150340W WO02052031A3 WO 2002052031 A3 WO2002052031 A3 WO 2002052031A3 US 0150340 W US0150340 W US 0150340W WO 02052031 A3 WO02052031 A3 WO 02052031A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
acid amplification
amplification
oligonucleotides
Prior art date
Application number
PCT/US2001/050340
Other languages
French (fr)
Other versions
WO2002052031A2 (en
Inventor
Mark G Erlander
Ranelle C Salunga
Original Assignee
Arcturus Eng Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcturus Eng Inc filed Critical Arcturus Eng Inc
Priority to AU2002245184A priority Critical patent/AU2002245184A1/en
Priority to EP01993343A priority patent/EP1343908A4/en
Publication of WO2002052031A2 publication Critical patent/WO2002052031A2/en
Publication of WO2002052031A3 publication Critical patent/WO2002052031A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Abstract

The present invention provides methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.
PCT/US2001/050340 2000-12-22 2001-12-21 Nucleic acid amplification WO2002052031A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002245184A AU2002245184A1 (en) 2000-12-22 2001-12-21 Nucleic acid amplification
EP01993343A EP1343908A4 (en) 2000-12-22 2001-12-21 Nucleic acid amplification

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US25780100P 2000-12-22 2000-12-22
US60/257,801 2000-12-22
US29884701P 2001-06-15 2001-06-15
US60/298,847 2001-06-15
US10/062,857 2001-10-25
US10/062,857 US6794141B2 (en) 2000-12-22 2001-10-25 Nucleic acid amplification

Publications (2)

Publication Number Publication Date
WO2002052031A2 WO2002052031A2 (en) 2002-07-04
WO2002052031A3 true WO2002052031A3 (en) 2002-09-12

Family

ID=27370385

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050340 WO2002052031A2 (en) 2000-12-22 2001-12-21 Nucleic acid amplification

Country Status (4)

Country Link
US (7) US6794141B2 (en)
EP (1) EP1343908A4 (en)
AU (1) AU2002245184A1 (en)
WO (1) WO2002052031A2 (en)

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Also Published As

Publication number Publication date
EP1343908A2 (en) 2003-09-17
US10036060B2 (en) 2018-07-31
US20030022194A1 (en) 2003-01-30
WO2002052031A2 (en) 2002-07-04
US20210062244A1 (en) 2021-03-04
US20110086394A1 (en) 2011-04-14
US20150119291A1 (en) 2015-04-30
US20050130194A1 (en) 2005-06-16
US20120322113A1 (en) 2012-12-20
EP1343908A4 (en) 2005-01-12
US20190002957A1 (en) 2019-01-03
AU2002245184A1 (en) 2002-07-08
US6794141B2 (en) 2004-09-21

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