WO2002050512A3 - Method of characterizing potential therapeutics by determining cell-cell interactions - Google Patents

Method of characterizing potential therapeutics by determining cell-cell interactions Download PDF

Info

Publication number
WO2002050512A3
WO2002050512A3 PCT/US2001/047820 US0147820W WO0250512A3 WO 2002050512 A3 WO2002050512 A3 WO 2002050512A3 US 0147820 W US0147820 W US 0147820W WO 0250512 A3 WO0250512 A3 WO 0250512A3
Authority
WO
WIPO (PCT)
Prior art keywords
agent
cell
cell types
images
interact
Prior art date
Application number
PCT/US2001/047820
Other languages
French (fr)
Other versions
WO2002050512A2 (en
Inventor
Kathleen A Elias
Original Assignee
Cytokinetics Inc
Kathleen A Elias
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytokinetics Inc, Kathleen A Elias filed Critical Cytokinetics Inc
Priority to EP01990116A priority Critical patent/EP1346200A2/en
Priority to AU2002228986A priority patent/AU2002228986A1/en
Priority to GB0316706A priority patent/GB2387654B/en
Publication of WO2002050512A2 publication Critical patent/WO2002050512A2/en
Publication of WO2002050512A3 publication Critical patent/WO2002050512A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G01N15/149—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1488—Methods for deciding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1497—Particle shape

Abstract

A method quantitatively analyzes images of two different cell types that interact in producing and maintaining a disease state or other biological condition. The two separate cell types are exposed to an agent or stimulus suspected of influencing the biological condition (e.g., the agent might be a potential therapeutic for treating a cancer). The two different cell types are co-cultured or otherwise allowed to interact with one another before and during exposure to the agent. The images of the cells show how the agent affects the cells' phenotypes, including their viability, migration patterns, etc. The method generates a quantitative phenotype for each cell type by quantitatively analyzing the cell images via an automatic procedure. The quantitative phenotypes typically take the form of a group of scalar or vector descriptors that together provide a 'fingerprint.' The descriptors may be size values, positions, morphological values, intensity distributions, etc.
PCT/US2001/047820 2000-12-18 2001-12-12 Method of characterizing potential therapeutics by determining cell-cell interactions WO2002050512A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01990116A EP1346200A2 (en) 2000-12-18 2001-12-12 Method of characterizing potential therapeutics by determining cell-cell interactions
AU2002228986A AU2002228986A1 (en) 2000-12-18 2001-12-12 Method of characterizing potential therapeutics by determining cell-cell interactions
GB0316706A GB2387654B (en) 2000-12-18 2001-12-12 Method of characterizing potential therapeutics by determining cell-cell interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/741,721 2000-12-18
US09/741,721 US6599694B2 (en) 2000-12-18 2000-12-18 Method of characterizing potential therapeutics by determining cell-cell interactions

Publications (2)

Publication Number Publication Date
WO2002050512A2 WO2002050512A2 (en) 2002-06-27
WO2002050512A3 true WO2002050512A3 (en) 2003-03-13

Family

ID=24981887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/047820 WO2002050512A2 (en) 2000-12-18 2001-12-12 Method of characterizing potential therapeutics by determining cell-cell interactions

Country Status (5)

Country Link
US (3) US6599694B2 (en)
EP (1) EP1346200A2 (en)
AU (1) AU2002228986A1 (en)
GB (1) GB2387654B (en)
WO (1) WO2002050512A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7151847B2 (en) 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
WO2002000940A2 (en) * 2000-06-23 2002-01-03 Cytokinetics, Inc Image analysis for phenotyping sets of mutant cells
US7016787B2 (en) 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
US7050620B2 (en) * 2001-03-30 2006-05-23 Heckman Carol A Method of assaying shape and structural features in cells
AU2003225508A1 (en) * 2002-05-17 2003-12-02 Pfizer Products Inc. Apparatus and method for statistical image analysis
US20050014217A1 (en) * 2003-07-18 2005-01-20 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
US7235353B2 (en) * 2003-07-18 2007-06-26 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
GB2423988A (en) * 2003-07-18 2006-09-13 Cytokinetics Inc Characterizing biological stimuli by response curves
US7479112B2 (en) * 2003-08-26 2009-01-20 Cardiac Pacemakers, Inc. Acoustic physiological sensor
US20050246105A1 (en) * 2004-01-28 2005-11-03 Vance Faber Interpolated image response
US8953866B2 (en) * 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells
WO2006060737A2 (en) * 2004-12-03 2006-06-08 Takeda San Diego, Inc. Mitotic kinesin inhibitors
JP4214124B2 (en) * 2005-03-14 2009-01-28 株式会社バイオエコーネット Ear thermometer
JP4515332B2 (en) * 2005-05-30 2010-07-28 オリンパス株式会社 Image processing apparatus and target area tracking program
KR100718158B1 (en) * 2006-04-21 2007-05-14 삼성전자주식회사 Cell co-culture device
EP2031528A4 (en) * 2006-05-26 2009-06-17 Univ Kyoto Estimation of protein-compound interaction and rational design of compound library based on chemical genomic information
EP2118268B1 (en) * 2007-01-10 2015-07-08 Hemoshear, LLC Use of an in vitro hemodynamic endothelial/smooth muscle cell co-culture model to identify new therapeutic targets for vascular disease
US8679836B2 (en) * 2007-10-01 2014-03-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of monitoring angiogenesis and metastasis in three dimensional co-cultures
US8031924B2 (en) * 2007-11-30 2011-10-04 General Electric Company Methods and systems for removing autofluorescence from images
DE102008026665A1 (en) * 2008-06-04 2009-12-17 Dr. Lerche Kg Process for and material of a molding standard
FR2935802B1 (en) * 2008-09-05 2012-12-28 Horiba Abx Sas METHOD AND DEVICE FOR CLASSIFYING, VISUALIZING AND EXPLORING BIOLOGICAL DATA
EP2686688B1 (en) 2011-03-17 2019-05-08 Cernostics, Inc. Systems and compositions for diagnosing barrett's esophagus and methods of using the same
CA2908622A1 (en) 2012-04-18 2013-10-24 Hemoshear, Llc In vitro model for pathological or physiologic conditions
EP3060654B1 (en) 2013-10-21 2023-03-15 Hemoshear, LLC In vitro model for a tumor microenvironment
JP6746945B2 (en) * 2015-06-30 2020-08-26 ソニー株式会社 Information processing apparatus, information processing system, and information processing method
WO2018110490A1 (en) * 2016-12-12 2018-06-21 日本電気株式会社 Information processing device, genetic information creation method, and program
US20230329630A1 (en) * 2020-08-28 2023-10-19 Pfizer Inc. Computerized decision support tool and medical device for respiratory condition monitoring and care

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
US5976826A (en) * 1993-10-04 1999-11-02 President And Fellows Of Harvard College Device containing cytophilic islands that adhere cells separated by cytophobic regions
WO2000017624A2 (en) * 1998-09-22 2000-03-30 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE455736B (en) 1984-03-15 1988-08-01 Sarastro Ab PROCEDURE KIT AND MICROPHOTOMETRATION AND ADDITIONAL IMAGE COMPOSITION
EP0184600B1 (en) 1984-12-10 1990-03-14 Prutec Limited Method for optically ascertaining parameters of species in a liquid analyte
US4741043B1 (en) 1985-11-04 1994-08-09 Cell Analysis Systems Inc Method of and apparatus for image analyses of biological specimens
US5016283A (en) 1985-11-04 1991-05-14 Cell Analysis Systems, Inc. Methods and apparatus for immunoploidy analysis
US4922092A (en) 1986-11-26 1990-05-01 Image Research Limited High sensitivity optical imaging apparatus
US4965725B1 (en) 1988-04-08 1996-05-07 Neuromedical Systems Inc Neural network based automated cytological specimen classification system and method
US4959301A (en) 1988-04-22 1990-09-25 Massachusetts Institute Of Technology Process for rapidly enumerating viable entities
US5162990A (en) * 1990-06-15 1992-11-10 The United States Of America As Represented By The United States Navy System and method for quantifying macrophage phagocytosis by computer image analysis
JP2510771B2 (en) 1990-07-25 1996-06-26 株式会社日立製作所 Method and system for diagnosing activity of cultured organism
US5235522A (en) 1990-10-10 1993-08-10 Cell Analysis Systems, Inc. Method and apparatus for automated analysis of biological specimens
US5784162A (en) 1993-08-18 1998-07-21 Applied Spectral Imaging Ltd. Spectral bio-imaging methods for biological research, medical diagnostics and therapy
US5991028A (en) 1991-02-22 1999-11-23 Applied Spectral Imaging Ltd. Spectral bio-imaging methods for cell classification
US5790710A (en) 1991-07-12 1998-08-04 Jeffrey H. Price Autofocus system for scanning microscopy
US5548661A (en) 1991-07-12 1996-08-20 Price; Jeffrey H. Operator independent image cytometer
US5326691A (en) * 1991-11-21 1994-07-05 John Hozier Micro-libraries and methods of making and manipulating them methods for generating and analyzing micro-libraries
US5655028A (en) 1991-12-30 1997-08-05 University Of Iowa Research Foundation Dynamic image analysis system
US5355215A (en) 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
US6026174A (en) 1992-10-14 2000-02-15 Accumed International, Inc. System and method for automatically detecting malignant cells and cells having malignancy-associated changes
GB9223259D0 (en) 1992-11-06 1992-12-23 Quatro Biosystems Ltd Method and apparatus for image analysis
US5733721A (en) 1992-11-20 1998-03-31 The Board Of Regents Of The University Of Oklahoma Cell analysis method using quantitative fluorescence image analysis
US6690817B1 (en) 1993-08-18 2004-02-10 Applied Spectral Imaging Ltd. Spectral bio-imaging data for cell classification using internal reference
US5352613A (en) 1993-10-07 1994-10-04 Tafas Triantafillos P Cytological screening method
DK0745243T3 (en) 1994-02-14 2004-04-19 Autocyte North Carolina Llc Methods for automated classification of cytological specimens
US5665028A (en) * 1994-02-23 1997-09-09 Nissan Motor Co., Ltd. Shift capacity control for automatic transmission
US5932872A (en) 1994-07-01 1999-08-03 Jeffrey H. Price Autofocus system for scanning microscopy having a volume image formation
US5790692A (en) 1994-09-07 1998-08-04 Jeffrey H. Price Method and means of least squares designed filters for image segmentation in scanning cytometry
US5902732A (en) 1995-10-04 1999-05-11 Cytoscan Sciences Llc Drug screening process measuring changes in cell volume
US6151405A (en) 1996-11-27 2000-11-21 Chromavision Medical Systems, Inc. System and method for cellular specimen grading
US6165734A (en) 1995-12-12 2000-12-26 Applied Spectral Imaging Ltd. In-situ method of analyzing cells
US5699428A (en) * 1996-01-16 1997-12-16 Symantec Corporation System for automatic decryption of file data on a per-use basis and automatic re-encryption within context of multi-threaded operating system under which applications run in real-time
US5893095A (en) 1996-03-29 1999-04-06 Virage, Inc. Similarity engine for content-based retrieval of images
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US6103479A (en) 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US5804436A (en) 1996-08-02 1998-09-08 Axiom Biotechnologies, Inc. Apparatus and method for real-time measurement of cellular response
US6008010A (en) 1996-11-01 1999-12-28 University Of Pittsburgh Method and apparatus for holding cells
JP4663824B2 (en) 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド Multiplexed molecular analyzer and method
US5995143A (en) 1997-02-07 1999-11-30 Q3Dm, Llc Analog circuit for an autofocus microscope system
DE69839501D1 (en) 1997-02-27 2008-06-26 Cellomics Inc System for screening biological cells
EP1002297A1 (en) 1997-08-07 2000-05-24 Imaging Research, Inc. A digital imaging system for assays in well plates, gels and blots
ATE282860T1 (en) 1997-09-11 2004-12-15 Randox Lab Ltd METHOD AND DEVICE FOR IMAGE ANALYSIS
GB9720987D0 (en) 1997-10-01 1997-12-03 Biocure Ltd A multicellular in vitro assay of angiogenesis
US5962250A (en) 1997-10-28 1999-10-05 Glaxo Group Limited Split multi-well plate and methods
AU2241899A (en) 1998-01-29 1999-08-16 Ribozyme Pharmaceuticals, Inc. Image acquisition and image analysis of biological material
EP1066517B1 (en) 1998-02-25 2006-11-29 THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES Cellular arrays for rapid molecular profiling
US5962520A (en) 1998-04-02 1999-10-05 The University Of Akron Hydrolytically unstable, biocompatible polymer
DK1095277T3 (en) 1998-07-13 2003-04-22 Cellomics Inc Cell-based screening system
AU6048599A (en) 1998-09-18 2000-04-10 Cellomics, Inc. A system for cell-based screening
US6146830A (en) 1998-09-23 2000-11-14 Rosetta Inpharmatics, Inc. Method for determining the presence of a number of primary targets of a drug
AU759180B2 (en) 1998-10-30 2003-04-10 Cellomics, Inc. A system for cell-based screening
IL127359A0 (en) 1998-12-01 1999-10-28 Yeda Res & Dev Computerized adaptive imaging
US6222093B1 (en) 1998-12-28 2001-04-24 Rosetta Inpharmatics, Inc. Methods for determining therapeutic index from gene expression profiles
CA2363020A1 (en) 1999-02-19 2000-08-24 Jian Wang Method and system for dynamic storage retrieval and analysis of experimental data with determined relationships
CA2368773A1 (en) 1999-04-01 2000-10-12 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
AU5444800A (en) * 1999-05-27 2000-12-18 Research Foundation Of State University Of New York, The LTiGTin vitroLT/iGT cell culture device including cartilage and methods of usingthe same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976826A (en) * 1993-10-04 1999-11-02 President And Fellows Of Harvard College Device containing cytophilic islands that adhere cells separated by cytophobic regions
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO2000017624A2 (en) * 1998-09-22 2000-03-30 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BLANKENSTEIN G ET AL: "MODULAR CONCEPT OF A LABORATORY ON A CHIP FOR CHEMICAL AND BIOCHEMICAL ANALYSIS", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 13, no. 3/4, 1998, pages 427 - 438, XP000700154, ISSN: 0956-5663 *
TAKAYAMA S ET AL: "Patterning of cells and their environments using multiple laminar fluid flows in capillary networks", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, May 1999 (1999-05-01), pages 5545 - 5548, XP002159130, ISSN: 0027-8424 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis
US8597899B2 (en) 2006-05-17 2013-12-03 Cernostics, Inc. Method for automated tissue analysis

Also Published As

Publication number Publication date
GB2387654A (en) 2003-10-22
US6599694B2 (en) 2003-07-29
GB2387654B (en) 2004-08-18
US20020119441A1 (en) 2002-08-29
AU2002228986A1 (en) 2002-07-01
WO2002050512A2 (en) 2002-06-27
EP1346200A2 (en) 2003-09-24
US20050208471A1 (en) 2005-09-22
GB0316706D0 (en) 2003-08-20
US20020160442A1 (en) 2002-10-31

Similar Documents

Publication Publication Date Title
WO2002050512A3 (en) Method of characterizing potential therapeutics by determining cell-cell interactions
Pijuan et al. In vitro cell migration, invasion, and adhesion assays: from cell imaging to data analysis
Brückner et al. Aggrecan-based extracellular matrix is an integral part of the human basal ganglia circuit
Wykoff et al. The motorneuron surface
Epp et al. Hippocampus-dependent learning promotes survival of new neurons in the dentate gyrus at a specific time during cell maturation
Glezer et al. Comparative analysis of calcium-binding protein-immunoreactive neuronal populations in the auditory and visual systems of the bottlenose dolphin (Tursiops truncatus) and the macaque monkey (Macaca fascicularis)
Mitchell et al. Large‐scale maintenance of dual projections by callosal and frontal cortical projection neurons in adult mice
Shenk et al. Expression of Cnox-2, a HOM/HOX gene, is suppressed during head formation in Hydra
Higo et al. Long‐range GABAergic projection neurons in the cat neocortex
Gilabert‐Juan et al. Reduced interneuronal dendritic arborization in CA1 but not in CA3 region of mice subjected to chronic mild stress
Federer et al. Two projection streams from macaque V1 to the pale cytochrome oxidase stripes of V2
Brauer et al. The core–shell dichotomy of nucleus accumbens in the rhesus monkey as revealed by double-immunofluorescence and morphology of cholinergic interneurons
Gavrilovici et al. Diverse interneuron populations have highly specific interconnectivity in the rat piriform cortex
Miki et al. Application of the physical disector to the central nervous system: estimation of the total number of neurons in subdivisions of the rat hippocampus
Nguyen et al. Effects of noise-induced hearing loss on parvalbumin and perineuronal net expression in the mouse primary auditory cortex
Kántor et al. Bipolar cell gap junctions serve major signaling pathways in the human retina
García et al. Visual estimation of the percentage of DNA in the tail in the comet assay: Evaluation of different approaches in an intercomparison exercise
Wolf et al. Evaluation of the swimming activity of Daphnia magna by image analysis after administration of sublethal cadmium concentrations
Holm et al. Quantification of vesicular zinc in the rat brain
Rajkovic et al. Novel application of the gray-level co-occurrence matrix analysis in the parvalbumin stained hippocampal gyrus dentatus in distinct rat models of Parkinson’s disease
Phillips Monitoring neuron and astrocyte interactions with a 3D cell culture system
Rodman et al. The Feulgen banded karyotype of the mouse: analysis of the mechanisms of banding
Döbrössy et al. Morphological and cellular changes within embryonic striatal grafts associated with enriched environment and involuntary exercise
Gracia‐Llanes et al. VIP‐containing deep short‐axon cells of the olfactory bulb innervate interneurons different from granule cells
Bywalez et al. Dendritic arborization patterns of small juxtaglomerular cell subtypes within the rodent olfactory bulb

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref document number: 0316706

Country of ref document: GB

Kind code of ref document: A

Free format text: PCT FILING DATE = 20011212

Format of ref document f/p: F

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2001990116

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001990116

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2001990116

Country of ref document: EP