WO2002048401A3 - Nested oligonucleotides containing hairpin for nucleic acid amplification - Google Patents
Nested oligonucleotides containing hairpin for nucleic acid amplification Download PDFInfo
- Publication number
- WO2002048401A3 WO2002048401A3 PCT/US2001/047727 US0147727W WO0248401A3 WO 2002048401 A3 WO2002048401 A3 WO 2002048401A3 US 0147727 W US0147727 W US 0147727W WO 0248401 A3 WO0248401 A3 WO 0248401A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- engineered
- nucleic acid
- acid amplification
- oligonucleotides containing
- containing hairpin
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01990978A EP1366191A2 (en) | 2000-12-11 | 2001-12-10 | Nested oligonucleotides containing hairpin for nucleic acid amplification |
AU2002230734A AU2002230734A1 (en) | 2000-12-11 | 2001-12-10 | Nested oligonucleotides containing hairpin for nucleic acid amplification |
CA002436693A CA2436693A1 (en) | 2000-12-11 | 2001-12-10 | Nested oligonucleotides containing hairpin for nucleic acid amplification |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25466900P | 2000-12-11 | 2000-12-11 | |
US60/254,669 | 2000-12-11 | ||
US32340001P | 2001-09-19 | 2001-09-19 | |
US60/323,400 | 2001-09-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002048401A2 WO2002048401A2 (en) | 2002-06-20 |
WO2002048401A3 true WO2002048401A3 (en) | 2003-09-12 |
Family
ID=26944185
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/047727 WO2002048401A2 (en) | 2000-12-11 | 2001-12-10 | Nested oligonucleotides containing hairpin for nucleic acid amplification |
Country Status (5)
Country | Link |
---|---|
US (3) | US6919189B2 (en) |
EP (1) | EP1366191A2 (en) |
AU (1) | AU2002230734A1 (en) |
CA (1) | CA2436693A1 (en) |
WO (1) | WO2002048401A2 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002092770A2 (en) * | 2001-05-14 | 2002-11-21 | Henry Hongjun Ji | Novel method for cloning variable domain sequences of immunological gene repertoire |
DK1436404T3 (en) * | 2001-09-19 | 2010-03-08 | Alexion Pharma Inc | Manipulated templates and their use in single-primer amplification |
WO2005027846A2 (en) * | 2003-09-18 | 2005-03-31 | Alexion Pharmaceuticals, Inc. | Elicitation of antibodies to self peptides in mice by immunization with dendritic cells |
US20050208627A1 (en) * | 2003-09-18 | 2005-09-22 | Bowdish Katherine S | Elicitation of antibodies to self peptides in mice by immunization with dendritic cells |
DK1778867T3 (en) | 2004-07-01 | 2010-08-02 | Gen Probe Inc | Methods and compositions for detecting nucleic acids in a biological sample |
US7833716B2 (en) * | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
CA2744004A1 (en) | 2008-11-25 | 2010-06-17 | Gen-Probe Incorporated | Compositions and methods for detecting small rnas, and uses thereof |
JP2015536672A (en) | 2012-11-28 | 2015-12-24 | エービーウィズ バイオ,インク. | Preparation of gene-specific templates for use in single primer amplification |
CN106811459B (en) * | 2015-11-27 | 2019-09-27 | 清华大学 | A method of it is checked using short strand primer and improves DNA clone and packaging efficiency |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997004131A1 (en) * | 1995-07-21 | 1997-02-06 | Forsyth Dental Infirmary For Children | Single primer amplification of polynucleotide hairpins |
US5679512A (en) * | 1992-07-31 | 1997-10-21 | Behringwerke Ag | Method for introducing defined sequences at the 3'end of polynucleotides |
US6087099A (en) * | 1997-09-08 | 2000-07-11 | Myriad Genetics, Inc. | Method for sequencing both strands of a double stranded DNA in a single sequencing reaction |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR8108820A (en) | 1980-09-24 | 1982-08-24 | Cetus Corp | DIAGNOSTIC PROCESS AND PROBE |
EP0084796B1 (en) | 1982-01-22 | 1990-05-02 | Cetus Corporation | Hla typing method and cdna probes used therein |
US4582788A (en) | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
KR0184860B1 (en) | 1988-11-11 | 1999-04-01 | 메디칼 리써어치 카운실 | Single domain ligands receptors comprising said ligands methods for their production and use of said ligands |
CA2016842A1 (en) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Method for tapping the immunological repertoire |
US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
FR2724934B1 (en) | 1994-09-26 | 1997-01-24 | Bio Merieux | CHIMERIC OLIGONUCLEOTIDE AND ITS USE IN OBTAINING NUCLEIC ACID TRANSCRIPTS |
US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
US6277607B1 (en) * | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
KR100527265B1 (en) | 1999-09-13 | 2005-11-09 | 뉴젠 테크놀로지스 인코포레이티드 | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6582938B1 (en) * | 2001-05-11 | 2003-06-24 | Affymetrix, Inc. | Amplification of nucleic acids |
DK1436404T3 (en) * | 2001-09-19 | 2010-03-08 | Alexion Pharma Inc | Manipulated templates and their use in single-primer amplification |
-
2001
- 2001-12-10 AU AU2002230734A patent/AU2002230734A1/en not_active Abandoned
- 2001-12-10 US US10/014,012 patent/US6919189B2/en not_active Expired - Lifetime
- 2001-12-10 EP EP01990978A patent/EP1366191A2/en not_active Withdrawn
- 2001-12-10 WO PCT/US2001/047727 patent/WO2002048401A2/en not_active Application Discontinuation
- 2001-12-10 CA CA002436693A patent/CA2436693A1/en not_active Abandoned
-
2003
- 2003-07-28 US US10/628,109 patent/US20040101886A1/en not_active Abandoned
-
2009
- 2009-02-18 US US12/378,811 patent/USRE41365E1/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5679512A (en) * | 1992-07-31 | 1997-10-21 | Behringwerke Ag | Method for introducing defined sequences at the 3'end of polynucleotides |
WO1997004131A1 (en) * | 1995-07-21 | 1997-02-06 | Forsyth Dental Infirmary For Children | Single primer amplification of polynucleotide hairpins |
US6087099A (en) * | 1997-09-08 | 2000-07-11 | Myriad Genetics, Inc. | Method for sequencing both strands of a double stranded DNA in a single sequencing reaction |
Non-Patent Citations (1)
Title |
---|
ZEBEDEE S L ET AL: "HUMAN COMBINATORIAL ANTIBODY LIBRARIES TO HEPATITIS B SURFACE ANTIGEN", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, 1 April 1992 (1992-04-01), pages 3175 - 3179, XP002031594, ISSN: 0027-8424 * |
Also Published As
Publication number | Publication date |
---|---|
AU2002230734A1 (en) | 2002-06-24 |
US20050079489A1 (en) | 2005-04-14 |
EP1366191A2 (en) | 2003-12-03 |
US20040101886A1 (en) | 2004-05-27 |
CA2436693A1 (en) | 2002-06-20 |
WO2002048401A2 (en) | 2002-06-20 |
US6919189B2 (en) | 2005-07-19 |
USRE41365E1 (en) | 2010-06-01 |
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