WO2002022882A3 - Target activated nucleic acid biosensor and methods of using same - Google Patents

Target activated nucleic acid biosensor and methods of using same Download PDF

Info

Publication number
WO2002022882A3
WO2002022882A3 PCT/US2001/028835 US0128835W WO0222882A3 WO 2002022882 A3 WO2002022882 A3 WO 2002022882A3 US 0128835 W US0128835 W US 0128835W WO 0222882 A3 WO0222882 A3 WO 0222882A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
same
sensor molecules
target
Prior art date
Application number
PCT/US2001/028835
Other languages
French (fr)
Other versions
WO2002022882A2 (en
Inventor
Marty Stanton
David Epstein
Nobuko Hamaguchi
Original Assignee
Archemix Corp
Marty Stanton
David Epstein
Nobuko Hamaguchi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Archemix Corp, Marty Stanton, David Epstein, Nobuko Hamaguchi filed Critical Archemix Corp
Priority to CA002418724A priority Critical patent/CA2418724A1/en
Priority to JP2002527322A priority patent/JP2004527220A/en
Priority to AU2001290965A priority patent/AU2001290965A1/en
Priority to EP01971029A priority patent/EP1354062A2/en
Publication of WO2002022882A2 publication Critical patent/WO2002022882A2/en
Publication of WO2002022882A3 publication Critical patent/WO2002022882A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00385—Printing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00479—Means for mixing reactants or products in the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B01J2219/00662—Two-dimensional arrays within two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00691—Automatic using robots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Composite Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Methods for engineering a target activated biosensor are provided. Biosensors comprise a plurality of nucleic acid sensor molecules labeled with a first signaling moiety and a second signaling moiety. The nucleic acid sensor molecules recognizes target molecules which do not naturally bind to DNA. Binding of a target molecule to the sensor molecules triggers a change in the proximity of the signaling moieties which leads to a change in the optical properties of the nucleic acid sensor molecules on the biosensor. Reagents and systems for performing the method are also provided. The method is useful in diagnostic applications and drug optimization.
PCT/US2001/028835 2000-09-13 2001-09-13 Target activated nucleic acid biosensor and methods of using same WO2002022882A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002418724A CA2418724A1 (en) 2000-09-13 2001-09-13 Target activated nucleic acid biosensor and methods of using same
JP2002527322A JP2004527220A (en) 2000-09-13 2001-09-13 Target activated nucleic acid biosensor and method of using same
AU2001290965A AU2001290965A1 (en) 2000-09-13 2001-09-13 Target activated nucleic acid biosensor and methods of using same
EP01971029A EP1354062A2 (en) 2000-09-13 2001-09-13 Target activated nucleic acid biosensor and methods of using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23245400P 2000-09-13 2000-09-13
US60/232,454 2000-09-13

Publications (2)

Publication Number Publication Date
WO2002022882A2 WO2002022882A2 (en) 2002-03-21
WO2002022882A3 true WO2002022882A3 (en) 2003-08-21

Family

ID=22873175

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/028835 WO2002022882A2 (en) 2000-09-13 2001-09-13 Target activated nucleic acid biosensor and methods of using same

Country Status (6)

Country Link
US (1) US20030087239A1 (en)
EP (1) EP1354062A2 (en)
JP (1) JP2004527220A (en)
AU (1) AU2001290965A1 (en)
CA (1) CA2418724A1 (en)
WO (1) WO2002022882A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853376B2 (en) 2002-11-21 2014-10-07 Archemix Llc Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6974706B1 (en) * 2003-01-16 2005-12-13 University Of Florida Research Foundation, Inc. Application of biosensors for diagnosis and treatment of disease
US20050233459A1 (en) * 2003-11-26 2005-10-20 Melker Richard J Marker detection method and apparatus to monitor drug compliance
WO2001034024A1 (en) 1999-11-08 2001-05-17 University Of Florida Research Foundation, Inc. Marker detection method and apparatus to monitor drug compliance
US6706474B1 (en) 2000-06-27 2004-03-16 Board Of Trustees Of The University Of Illinois Nucleic acid enzyme biosensors for ions
US20050054942A1 (en) * 2002-01-22 2005-03-10 Melker Richard J. System and method for therapeutic drug monitoring
US6981947B2 (en) * 2002-01-22 2006-01-03 University Of Florida Research Foundation, Inc. Method and apparatus for monitoring respiratory gases during anesthesia
US7104963B2 (en) 2002-01-22 2006-09-12 University Of Florida Research Foundation, Inc. Method and apparatus for monitoring intravenous (IV) drug concentration using exhaled breath
GB0111459D0 (en) * 2001-05-10 2001-07-04 Isis Innovation Universal fluorescent sensors
US7052854B2 (en) * 2001-05-23 2006-05-30 University Of Florida Research Foundation, Inc. Application of nanotechnology and sensor technologies for ex-vivo diagnostics
WO2002095359A2 (en) * 2001-05-23 2002-11-28 University Of Florida Method and apparatus for detecting illicit substances
EP1393069A1 (en) * 2001-05-24 2004-03-03 The University Of Florida Method and apparatus for detecting environmental smoke exposure
WO2003027276A2 (en) * 2001-09-24 2003-04-03 University Of Aarhus Novel compositions and methods for diagnosis and treatment of lymphoma and leukemia
US20070167853A1 (en) * 2002-01-22 2007-07-19 Melker Richard J System and method for monitoring health using exhaled breath
US20030148391A1 (en) * 2002-01-24 2003-08-07 Salafsky Joshua S. Method using a nonlinear optical technique for detection of interactions involving a conformational change
CA2516091C (en) * 2002-03-11 2016-03-08 Simon Fraser University Dna and rna conformational switches as sensitive electronic sensors of analytes
US20030198966A1 (en) * 2002-04-19 2003-10-23 Stojanovic Milan N. Displacement assay for detection of small molecules
US7534560B2 (en) 2002-05-10 2009-05-19 The Board Of Trustees Of The University Of Illinois Simple catalytic DNA biosensors for ions based on color changes
US6890719B2 (en) 2002-05-10 2005-05-10 The Board Of Trustess Of The University Of Illinois Fluorescence based biosensor
JP4583007B2 (en) * 2002-07-16 2010-11-17 住友化学株式会社 Novel G-protein and use thereof
EP1382613A1 (en) * 2002-07-16 2004-01-21 Sumitomo Chemical Company, Limited Novel G protein, polynucleotide encoding the same and utilization thereof
EP1543158B1 (en) 2002-07-25 2011-07-13 Archemix Corp. Regulated aptamer therapeutics
WO2004034436A2 (en) * 2002-10-09 2004-04-22 Cellectricon Ab Method for interfacing macroscale components to microscale devices
US20060160134A1 (en) * 2002-10-21 2006-07-20 Melker Richard J Novel application of biosensors for diagnosis and treatment of disease
US10100316B2 (en) 2002-11-21 2018-10-16 Archemix Llc Aptamers comprising CPG motifs
US8039443B2 (en) 2002-11-21 2011-10-18 Archemix Corporation Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics
WO2004069850A2 (en) 2003-02-04 2004-08-19 Borer Philip N Switchable nucleic acids for diagnostics, screening and molecular electronics
US7612185B2 (en) 2003-03-07 2009-11-03 The Board Of Trustees Of The University Of Illinois Nucleic acid biosensors
EP1606416A2 (en) * 2003-03-10 2005-12-21 Casio Computer Co., Ltd. Dna analyzing apparatus, dna sensor, and analyzing method
US7470516B2 (en) * 2003-04-14 2008-12-30 The Trustees Of Columbia University In The City Of New York Cross reactive arrays of three-way junction sensors for steroid determination
US20040203007A1 (en) * 2003-04-14 2004-10-14 Stojanovic Milan N. Cross reactive arrays of three-way junction sensors for steroid determination
US7473548B2 (en) 2003-04-25 2009-01-06 Medtronic, Inc. Optical detector for enzyme activation
EP1644531A4 (en) * 2003-06-25 2009-03-11 Somagenics Inc Polynucleotides capable of target-dependent circularization and topological linkage
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
EP1514944A1 (en) * 2003-09-11 2005-03-16 Aventis Pharma Deutschland GmbH High-throughput-Assay with high sensitivity for measuring of the activity of beta-adrenergic receptor kinase and for determining the impact of test substances on such activity
US7279286B2 (en) 2003-09-11 2007-10-09 Sanofi-Aventis Deutschland Gmbh High-throughput-assay with high sensitivity for measuring of the activity of β-adrenergic receptor kinase and for determining the impact of test substances on such activity
US8080097B2 (en) * 2003-11-06 2011-12-20 Hewlett-Packard Development Company, L.P. System and a method for the creation of edible, optically invisible images
EP1692486B1 (en) * 2003-12-12 2015-10-28 Saint Louis University Biosensors for detecting macromolecules and other analytes
US7485419B2 (en) 2004-01-13 2009-02-03 The Board Of Trustees Of The University Of Illinois Biosensors based on directed assembly of particles
US20050191757A1 (en) * 2004-01-20 2005-09-01 Melker Richard J. Method and apparatus for detecting humans and human remains
JP4576945B2 (en) * 2004-02-09 2010-11-10 ソニー株式会社 Detection surface for detecting interaction between substances, sensor chip, sensor device and detection method using the detection surface
US20050255044A1 (en) * 2004-05-14 2005-11-17 Lomnes Stephen J Contrast agent for combined modality imaging and methods and systems thereof
EP1769088A2 (en) * 2004-06-30 2007-04-04 Whitehead Institute For Biomedical Research Novel methods for high-throughput genome-wide location analysis
CA2576511A1 (en) * 2004-08-03 2006-07-20 Syracuse University Branched and multi-chain nucleic acid switches for sensing and screening
US20060062734A1 (en) * 2004-09-20 2006-03-23 Melker Richard J Methods and systems for preventing diversion of prescription drugs
US20060240227A1 (en) * 2004-09-23 2006-10-26 Zhijun Zhang Nanocrystal coated surfaces
WO2006113610A2 (en) * 2005-04-19 2006-10-26 Syracuse University Rapid exchange luminescence (rel) for high sensitivity detection
US7947443B2 (en) * 2005-05-09 2011-05-24 Simon Fraser University DNA and RNA conformational switches as sensitive electronic sensors of analytes
US20060257883A1 (en) * 2005-05-10 2006-11-16 Bjoraker David G Detection and measurement of hematological parameters characterizing cellular blood components
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (en) 2005-06-10 2012-07-04 Saint Louis University Methods for the selection of aptamers
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
US7892734B2 (en) 2005-08-11 2011-02-22 The Board Of Trustees Of The University Of Illinois Aptamer based colorimetric sensor systems
US7799554B2 (en) 2006-03-16 2010-09-21 The Board Of Trustees Of The University Of Illinois Lateral flow devices
US20090017553A1 (en) * 2006-06-12 2009-01-15 Arizona Bd Of Regents/Behalf Of Univ. Of Arizona Immunoassay-based microsensing using optical sensors
US7914460B2 (en) 2006-08-15 2011-03-29 University Of Florida Research Foundation, Inc. Condensate glucose analyzer
JP4751302B2 (en) * 2006-11-21 2011-08-17 株式会社日立製作所 Potential difference sensor and analytical element
WO2008089248A2 (en) 2007-01-19 2008-07-24 The Board Of Trustees Of The University Of Illinois Amphiphilic substances and triggered liberation from lipid vesicles
US7680553B2 (en) * 2007-03-08 2010-03-16 Smp Logic Systems Llc Methods of interfacing nanomaterials for the monitoring and execution of pharmaceutical manufacturing processes
US8058415B2 (en) 2007-04-24 2011-11-15 The Board Of Trustees Of The University Of Illinois Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes
JP5223237B2 (en) * 2007-05-14 2013-06-26 ソニー株式会社 Method for detecting binding or interaction between nucleic acid strand for detection and substance
US8551786B2 (en) * 2007-07-09 2013-10-08 Fio Corporation Systems and methods for enhancing fluorescent detection of target molecules in a test sample
US8409800B2 (en) 2007-07-16 2013-04-02 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for copper detection
US8568690B2 (en) 2007-07-31 2013-10-29 The Board Of Trustees Of The University Of Illinois MRI contrast agents and high-throughput screening by MRI
US8367416B2 (en) 2007-08-10 2013-02-05 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for mercury detection
PT2209893E (en) * 2007-10-12 2014-02-26 Pronota Nv Use of aptamers in proteomics
US8062893B2 (en) 2008-10-10 2011-11-22 The Board Of Trustees Of The University Of Illinois Fluorescent sensor for mercury
EP2703816B1 (en) 2008-11-21 2016-10-05 Saint Louis University Biosensor for detecting multiple epitopes on a target
US20100305197A1 (en) * 2009-02-05 2010-12-02 Massachusetts Institute Of Technology Conditionally Active Ribozymes And Uses Thereof
DE102009058295A1 (en) * 2009-12-10 2011-06-16 Biostep Gmbh Trans-illuminator for e.g. rear illumination of fluorescent material sample for qualitative and quantitative evaluation of sample in research laboratory, has adjustment component for adjustment of specific wave length
WO2011100561A1 (en) 2010-02-12 2011-08-18 Saint Louis University Molecular biosensors capable of signal amplification
DE102010003781B4 (en) * 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Method for detecting specific nucleic acid sequences
KR101423588B1 (en) 2011-12-29 2014-07-30 주식회사 메디센서 Multiplex Diagnostic Apparatus and Analysis Method Using the Same
US9938560B2 (en) 2012-02-05 2018-04-10 Biodesy, Inc. Methods for identifying modulators of Ras using nonlinear techniques
WO2013119888A1 (en) 2012-02-09 2013-08-15 President And Fellows Of Harvard College Selective nucleic acid amplification from nucleic acid pools
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
ES2912033T3 (en) 2012-10-23 2022-05-24 Caris Science Inc Aptamers and uses thereof
EP2935628B1 (en) 2012-12-19 2018-03-21 Caris Life Sciences Switzerland Holdings GmbH Compositions and methods for aptamer screening
WO2014102803A1 (en) 2012-12-31 2014-07-03 Yeda Research And Development Co. Ltd. Molecular sensor and methods of use thereof
US10745746B2 (en) 2013-07-18 2020-08-18 President And Fellows Of Harvard College Specific nucleic acid amplification with compounded selectivity
JP2014016366A (en) * 2013-10-02 2014-01-30 Tsumura & Co Bioassay method for yi-gan san
US9986941B2 (en) 2014-06-26 2018-06-05 The Board Of Trustees Of The Leland Stanford Junior University Methods and apparatus for rapid monitoring of hemostatic state
WO2016040830A1 (en) 2014-09-12 2016-03-17 Mediomics, Llc Molecular biosensors with a modular design
EP3237906B8 (en) 2014-12-23 2020-10-28 Bluelight Therapeutics, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
US20180094309A1 (en) * 2015-04-03 2018-04-05 President And Fellows Of Harvard College Nucleic acid retro-activated primers
WO2017156238A1 (en) * 2016-03-11 2017-09-14 President And Fellows Of Harvard College Protein stability-based small molecule biosensors and methods
JP7221511B2 (en) * 2018-07-05 2023-02-14 学校法人福岡大学 Protein-targeting nucleic acid aptamer, method for producing same, and method for detecting target protein
CN110609078B (en) * 2019-09-20 2022-03-11 南京谱利健生物技术有限公司 Method for detecting protein phosphorylation and acetylglucosamine saccharification correlation effect

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998027104A1 (en) * 1996-12-19 1998-06-25 Yale University Bioreactive allosteric polynucleotides
WO1999031276A1 (en) * 1997-12-15 1999-06-24 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
WO2000070329A1 (en) * 1999-05-14 2000-11-23 Brandeis University Nucleic acid-based detection

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5652094A (en) * 1992-01-31 1997-07-29 University Of Montreal Nucleozymes
US6469158B1 (en) * 1992-05-14 2002-10-22 Ribozyme Pharmaceuticals, Incorporated Synthesis, deprotection, analysis and purification of RNA and ribozymes
US5496698A (en) * 1992-08-26 1996-03-05 Ribozyme Pharmaceuticals, Inc. Method of isolating ribozyme targets
US5686599A (en) * 1992-05-14 1997-11-11 Ribozyme Pharmaceuticals, Inc. Synthesis, deprotection, analysis and purification of RNA and ribozymes
US5977343A (en) * 1992-05-14 1999-11-02 Ribozyme Pharmaceuticals, Inc. Synthesis, deprotection, analysis and purification of RNA and ribozymes
US5646020A (en) * 1992-05-14 1997-07-08 Ribozyme Pharmaceuticals, Inc. Hammerhead ribozymes for preferred targets
US5750390A (en) * 1992-08-26 1998-05-12 Ribozyme Pharmaceuticals, Inc. Method and reagent for treatment of diseases caused by expression of the bcl-2 gene
US5610052A (en) * 1992-08-26 1997-03-11 Ribozyme Pharmaceuticals Inc. Enzymatic RNA with activity to ras
US5525468A (en) * 1992-05-14 1996-06-11 Ribozyme Pharmaceuticals, Inc. Assay for Ribozyme target site
US5891684A (en) * 1992-10-15 1999-04-06 Ribozyme Pharmaceuticals, Inc. Base-modified enzymatic nucleic acid
US5583020A (en) * 1992-11-24 1996-12-10 Ribozyme Pharmaceuticals, Inc. Permeability enhancers for negatively charged polynucleotides
US5616490A (en) * 1992-12-07 1997-04-01 Ribozyme Pharmaceuticals, Inc. Ribozymes targeted to TNF-α RNA
US5616488A (en) * 1992-12-07 1997-04-01 Ribozyme Pharmaceuticals, Inc. IL-5 targeted ribozymes
US5811300A (en) * 1992-12-07 1998-09-22 Ribozyme Pharmaceuticals, Inc. TNF-α ribozymes
JPH09502092A (en) * 1993-09-02 1997-03-04 リボザイム・ファーマシューティカルズ・インコーポレイテッド Enzymatic nucleic acid containing non-nucleotide
US5861288A (en) * 1993-10-18 1999-01-19 Ribozyme Pharmaceuticals, Inc. Catalytic DNA
CA2174339A1 (en) * 1993-10-27 1995-05-04 Lech W. Dudycz 2'-amido and 2'-peptido modified oligonucleotides
US5639647A (en) * 1994-03-29 1997-06-17 Ribozyme Pharmaceuticals, Inc. 2'-deoxy-2'alkylnucleotide containing nucleic acid
US5902880A (en) * 1994-08-19 1999-05-11 Ribozyme Pharmaceuticals, Inc. RNA polymerase III-based expression of therapeutic RNAs
US5631359A (en) * 1994-10-11 1997-05-20 Ribozyme Pharmaceuticals, Inc. Hairpin ribozymes
US5627053A (en) * 1994-03-29 1997-05-06 Ribozyme Pharmaceuticals, Inc. 2'deoxy-2'-alkylnucleotide containing nucleic acid
US6103890A (en) * 1994-05-18 2000-08-15 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids that cleave C-fos
US6146886A (en) * 1994-08-19 2000-11-14 Ribozyme Pharmaceuticals, Inc. RNA polymerase III-based expression of therapeutic RNAs
US5599706A (en) * 1994-09-23 1997-02-04 Stinchcomb; Dan T. Ribozymes targeted to apo(a) mRNA
US5716824A (en) * 1995-04-20 1998-02-10 Ribozyme Pharmaceuticals, Inc. 2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic nucleic acids (ribozymes)
US5672501A (en) * 1994-12-23 1997-09-30 Ribozyme Pharmaceuticals, Inc. Base-modified enzymatic nucleic acid
US5877021A (en) * 1995-07-07 1999-03-02 Ribozyme Pharmaceuticals, Inc. B7-1 targeted ribozymes
US5998203A (en) * 1996-04-16 1999-12-07 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids containing 5'-and/or 3'-cap structures
US5962675A (en) * 1996-02-13 1999-10-05 Ribozyme Pharmaceuticals, Inc. Chemical syntheses of 2'-O-methoxy purine nucleosides
US5807743A (en) * 1996-12-03 1998-09-15 Ribozyme Pharmaceuticals, Inc. Interleukin-2 receptor gamma-chain ribozymes
US6248878B1 (en) * 1996-12-24 2001-06-19 Ribozyme Pharmaceuticals, Inc. Nucleoside analogs
US6159951A (en) * 1997-02-13 2000-12-12 Ribozyme Pharmaceuticals Inc. 2'-O-amino-containing nucleoside analogs and polynucleotides
US6251666B1 (en) * 1997-03-31 2001-06-26 Ribozyme Pharmaceuticals, Inc. Nucleic acid catalysts comprising L-nucleotide analogs
US6183959B1 (en) * 1997-07-03 2001-02-06 Ribozyme Pharmaceuticals, Inc. Method for target site selection and discovery
AU750947C (en) * 1997-09-22 2003-05-22 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid catalysts with endonuclease activity
US6127535A (en) * 1997-11-05 2000-10-03 Ribozyme Pharmaceuticals, Inc. Nucleoside triphosphates and their incorporation into oligonucleotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998027104A1 (en) * 1996-12-19 1998-06-25 Yale University Bioreactive allosteric polynucleotides
WO1999031276A1 (en) * 1997-12-15 1999-06-24 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
WO2000070329A1 (en) * 1999-05-14 2000-11-23 Brandeis University Nucleic acid-based detection

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FITZWATER AND B POLISKY T: "A SELEX primer", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 267, 1996, pages 275 - 301, XP002112701, ISSN: 0076-6879 *
GOLDEN MACE C ET AL: "Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers.", JOURNAL OF BIOTECHNOLOGY, vol. 81, no. 2-3, 2000, pages 167 - 178, XP004210488, ISSN: 0168-1656 *
RINGQUIST STEVEN ET AL: "Anti-L-selectin oligonucleotide ligands recognize CD62L-positive leukocytes: Binding affinity and specificity of univalent and bivalent ligands.", CYTOMETRY, vol. 33, no. 4, 1 December 1998 (1998-12-01), pages 394 - 405, XP002927384, ISSN: 0196-4763 *
See also references of EP1354062A2 *
WALLACE MARK I ET AL: "FRET fluctuation spectroscopy: Unravelling the conformational motions of a DNA hairpin-loop.", EUROPEAN BIOPHYSICS JOURNAL, vol. 29, no. 4-5, 22 August 2000 (2000-08-22), 3rd European Biophysics Congress;Munchen, Germany; September 09-13, 2000, pages 254, XP002224715, ISSN: 0175-7571, Retrieved from the Internet <URL:http://link.springer.de/link/service/journals/00249/tocs/t0029004.htm> [retrieved on 20021211] *
YAMAMOTO RIKA ET AL: "Molecular beacon aptamer fluoresces in the presence of Tat protein of HIV-1.", GENES TO CELLS, vol. 5, no. 5, May 2000 (2000-05-01), pages 389 - 396, XP002224714, ISSN: 1356-9597 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853376B2 (en) 2002-11-21 2014-10-07 Archemix Llc Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics

Also Published As

Publication number Publication date
EP1354062A2 (en) 2003-10-22
WO2002022882A2 (en) 2002-03-21
US20030087239A1 (en) 2003-05-08
JP2004527220A (en) 2004-09-09
CA2418724A1 (en) 2002-03-21
AU2001290965A1 (en) 2002-03-26

Similar Documents

Publication Publication Date Title
WO2002022882A3 (en) Target activated nucleic acid biosensor and methods of using same
Xu et al. Solid-phase reversible immobilization in microfluidic chips for the purification of dye-labeled DNA sequencing fragments
WO2003043402A3 (en) Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
WO2004092342A3 (en) Lateral flow system for nucleic acid detection
ATE534748T1 (en) DIRECT SNP DETECTION USING NON-AMPLIFIED DNA
FR2726286B1 (en) SOLID PHASE NUCLEIC ACID AMPLIFICATION PROCESS AND REAGENT KIT USEFUL FOR CARRYING OUT SAID PROCESS
WO2001071041A3 (en) Microarray-based analysis of polynucleotide sequence variations
DE50015331D1 (en) PROCESS FOR CONNECTING NUCLEIC ACIDS TO A SOLID PHASE
WO2002061127A3 (en) Arrayed polynucleotides and their use in genome analysis
ATE377091T1 (en) METHOD FOR DETECTING NUCLEIC ACIDS
AU587188B2 (en) Nucleic acid hybridazation assay employing detectable anti- hybrid antibodies
WO2004046687A3 (en) Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates
WO2002020838A3 (en) Method and system for detecting nucleic acids
US10196672B2 (en) Affinity tag nucleic acid and protein compositions, and processes for using same
WO2002004655A3 (en) Cation mediated triplex hybridization assay
DK1436420T3 (en) Test Strip Assay System and Method for Detecting Specific Nucleic Acid Sequences
WO2003014375A3 (en) Nucleic acid sensor molecules and methods of using same
ATE242261T1 (en) FLUORESCENT CONJUGATES OF NUCLEOSIDES AND NUCLEOTIDES, THEIR PROCESS FOR PRODUCTION AND THEIR USES
DE60043618D1 (en) METHOD FOR DETECTING A MOLECULAR DETECTION RESPONSE
CA2409973A1 (en) Reverse detection for identification and/or quantification of nucleotide target sequences on biochips
WO2003072721A2 (en) Detection by sliding template amplification
WO2004013305A3 (en) Improved compositions for in vitro amplification of nucleic acids
US20210033606A1 (en) DNA mapping and sequencing on linearized DNA molecules
WO1998035058A3 (en) Improved process for detection and quantification of nucleic acid molecules
Sano et al. Deoxyribonucleic acids as unique markers in molecular detection

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2418724

Country of ref document: CA

Ref document number: 2001290965

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001971029

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002527322

Country of ref document: JP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 2001971029

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001971029

Country of ref document: EP