WO2002022667A3 - Twin-arginine translocation in bacillus - Google Patents

Twin-arginine translocation in bacillus Download PDF

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Publication number
WO2002022667A3
WO2002022667A3 PCT/US2001/029151 US0129151W WO0222667A3 WO 2002022667 A3 WO2002022667 A3 WO 2002022667A3 US 0129151 W US0129151 W US 0129151W WO 0222667 A3 WO0222667 A3 WO 0222667A3
Authority
WO
WIPO (PCT)
Prior art keywords
gram
host cell
bacillus
protein
secretion
Prior art date
Application number
PCT/US2001/029151
Other languages
French (fr)
Other versions
WO2002022667A9 (en
WO2002022667A2 (en
Inventor
Jan Douwe Harm Jongbloed
Joerg Paul Mueller
Dijl Jan Maarten Van
Sierd Bron
Original Assignee
Genencor Int
Jan Douwe Harm Jongbloed
Joerg Paul Mueller
Dijl Jan Maarten Van
Sierd Bron
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, Jan Douwe Harm Jongbloed, Joerg Paul Mueller, Dijl Jan Maarten Van, Sierd Bron filed Critical Genencor Int
Priority to DE60115958T priority Critical patent/DE60115958T2/en
Priority to AU2001292749A priority patent/AU2001292749A1/en
Priority to EP01973140A priority patent/EP1356060B1/en
Priority to AT01973140T priority patent/ATE312932T1/en
Publication of WO2002022667A2 publication Critical patent/WO2002022667A2/en
Publication of WO2002022667A9 publication Critical patent/WO2002022667A9/en
Publication of WO2002022667A3 publication Critical patent/WO2002022667A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Abstract

Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microoraganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
PCT/US2001/029151 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus WO2002022667A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE60115958T DE60115958T2 (en) 2000-09-18 2001-09-17 TWIN ARGININE TRANSLOCATION IN BACILLUS
AU2001292749A AU2001292749A1 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus
EP01973140A EP1356060B1 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus
AT01973140T ATE312932T1 (en) 2000-09-18 2001-09-17 TWIN ARGININE TRANSLOCATION IN BACILLUS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23361000P 2000-09-18 2000-09-18
US60/233,610 2000-09-18

Publications (3)

Publication Number Publication Date
WO2002022667A2 WO2002022667A2 (en) 2002-03-21
WO2002022667A9 WO2002022667A9 (en) 2003-03-20
WO2002022667A3 true WO2002022667A3 (en) 2003-09-04

Family

ID=22877967

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/029151 WO2002022667A2 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus

Country Status (7)

Country Link
US (4) US7316924B2 (en)
EP (1) EP1356060B1 (en)
AT (1) ATE312932T1 (en)
AU (1) AU2001292749A1 (en)
DE (1) DE60115958T2 (en)
DK (1) DK1356060T3 (en)
WO (1) WO2002022667A2 (en)

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US7655249B2 (en) * 1998-09-30 2010-02-02 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7329410B1 (en) 1998-09-30 2008-02-12 Corixa Corporation Compositions and method for WT1 specific immunotherapy
US7901693B2 (en) * 1998-09-30 2011-03-08 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7553494B2 (en) * 2001-08-24 2009-06-30 Corixa Corporation WT1 fusion proteins
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
WO2003040335A2 (en) * 2001-11-05 2003-05-15 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
WO2003083056A2 (en) * 2002-03-23 2003-10-09 Research Development Foundation Secretion of proteins with multiple disulfide bonds in bacteria and uses thereof
DE10256669B4 (en) * 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Mixture of at least two fusion proteins and their preparation and use
JP4730302B2 (en) 2004-04-20 2011-07-20 味の素株式会社 Protein production method
US20070026012A1 (en) * 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
EP1917347B1 (en) * 2005-08-22 2015-10-07 Cornell Research Foundation, Inc. Compositions and methods for analyzing protein interactions
KR100742218B1 (en) 2005-10-21 2007-07-24 학교법인 포항공과대학교 Secretion of recombinant target protein in Escherichia coli using the secretion signal of organophosphorus hydrolase having twin arginine translocation pathway
EP2426199A3 (en) 2006-10-20 2012-08-22 Danisco US Inc. Polyol oxidases
WO2008065200A1 (en) 2006-11-30 2008-06-05 Novozymes A/S Dnase expression in recombinant host cells
WO2008089132A2 (en) * 2007-01-12 2008-07-24 Cornell Research Foundation, Inc. Genetic selection for protein folding and solubility in the bacterial periplasm
US20100285567A1 (en) * 2007-06-18 2010-11-11 Kai Bao Tat signal peptides for producing proteins in prokaryotes
DE102008025926A1 (en) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Secretion-optimized microorganism
DE102008025791A1 (en) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Secretion-optimized microorganism
SI3044320T1 (en) * 2013-09-11 2020-07-31 Impossible Foods Inc. Secretion of heme-containing polypeptides
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
NZ737094A (en) 2015-05-11 2019-06-28 Impossible Foods Inc Expression constructs and methods of genetically engineering methylotrophic yeast
KR101791526B1 (en) * 2016-02-18 2017-11-01 (주)케어젠 Peptides having Hair Growth Activity and Uses Thereof
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
GB201713732D0 (en) * 2017-08-25 2017-10-11 Alta Innovations Ltd Tat expression system
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

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WO1999051753A1 (en) * 1998-04-01 1999-10-14 The Governors Of The University Of Alberta Compositions and methods for protein secretion
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Also Published As

Publication number Publication date
US20080166757A1 (en) 2008-07-10
US20090093025A1 (en) 2009-04-09
WO2002022667A9 (en) 2003-03-20
US7884191B2 (en) 2011-02-08
DE60115958T2 (en) 2006-08-03
EP1356060A2 (en) 2003-10-29
US20080248525A1 (en) 2008-10-09
DE60115958D1 (en) 2006-01-19
US7884192B2 (en) 2011-02-08
US7316924B2 (en) 2008-01-08
WO2002022667A2 (en) 2002-03-21
EP1356060B1 (en) 2005-12-14
ATE312932T1 (en) 2005-12-15
US7897742B2 (en) 2011-03-01
DK1356060T3 (en) 2006-05-01
AU2001292749A1 (en) 2002-03-26
US20020110860A1 (en) 2002-08-15

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