WO2002015949A3 - Device for identifying the presence of a nucleotide sequence in a dna sample - Google Patents

Device for identifying the presence of a nucleotide sequence in a dna sample Download PDF

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Publication number
WO2002015949A3
WO2002015949A3 PCT/US2001/026555 US0126555W WO0215949A3 WO 2002015949 A3 WO2002015949 A3 WO 2002015949A3 US 0126555 W US0126555 W US 0126555W WO 0215949 A3 WO0215949 A3 WO 0215949A3
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WO
WIPO (PCT)
Prior art keywords
sample
chamber
nucleotide
dna sample
membrane
Prior art date
Application number
PCT/US2001/026555
Other languages
French (fr)
Other versions
WO2002015949A9 (en
WO2002015949A2 (en
WO2002015949A8 (en
Inventor
James Benn
Jean-Francois Manchec
Original Assignee
Genome Therapeutics Corp
James Benn
Jean-Francois Manchec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genome Therapeutics Corp, James Benn, Jean-Francois Manchec filed Critical Genome Therapeutics Corp
Priority to AU2001286756A priority Critical patent/AU2001286756A1/en
Publication of WO2002015949A2 publication Critical patent/WO2002015949A2/en
Publication of WO2002015949A8 publication Critical patent/WO2002015949A8/en
Publication of WO2002015949A3 publication Critical patent/WO2002015949A3/en
Publication of WO2002015949A9 publication Critical patent/WO2002015949A9/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • G01N2001/4016—Concentrating samples by transferring a selected component through a membrane being a selective membrane, e.g. dialysis or osmosis

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

A system and method of identifying the presence of specific nucleotide sequences in a target DNA sample is provided. The nucleotide detection system comprises a flat plat detection cell having a sample chamber, a membrane and an optical window providing optical access to the interior of the cell. A target DNA sample is mixed with labeled nucleotides and other chemistries, and undergoes a chemical reaction such that if the DNA sample has the nucleotide, the resulting mixture will contain labeled nucleotides that have undergone a change in molecular weight. The reacted sample is applied to the sample chamber and the membrane in the flat plate nucleotide detection system is utilized to effect the separation of the smaller molecular weight labels from the sample. After separation, the presence of the label in either the sample chamber or the filtrate chamber, or on the membrane itself, is detected through the optical window to the sample chamber or through an optical window to the filtrate chamber to determine the presence or absence of the nucleotide sequence.
PCT/US2001/026555 2000-08-25 2001-08-24 Device for identifying the presence of a nucleotide sequence in a dna sample WO2002015949A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001286756A AU2001286756A1 (en) 2000-08-25 2001-08-24 Device for identifying the presence of a nucleotide sequence in a dna sample

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22823900P 2000-08-25 2000-08-25
US60/228,239 2000-08-25
US26603501P 2001-02-02 2001-02-02
US60/266,035 2001-02-02

Publications (4)

Publication Number Publication Date
WO2002015949A2 WO2002015949A2 (en) 2002-02-28
WO2002015949A8 WO2002015949A8 (en) 2002-07-04
WO2002015949A3 true WO2002015949A3 (en) 2002-10-03
WO2002015949A9 WO2002015949A9 (en) 2003-03-27

Family

ID=26922164

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2001/026611 WO2002016652A2 (en) 2000-08-25 2001-08-24 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
PCT/US2001/026555 WO2002015949A2 (en) 2000-08-25 2001-08-24 Device for identifying the presence of a nucleotide sequence in a dna sample

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2001/026611 WO2002016652A2 (en) 2000-08-25 2001-08-24 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification

Country Status (2)

Country Link
AU (2) AU2001286756A1 (en)
WO (2) WO2002016652A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7081339B2 (en) 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
US7445893B2 (en) 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
AU2003291144A1 (en) * 2002-11-21 2004-06-18 Primera Biosystems Sampling method and apparatus for amplification reaction analysis
US7250260B2 (en) 2004-02-18 2007-07-31 Applera Corporation Multi-step bioassays on modular microfluidic application platforms
DE102005010096B3 (en) 2005-03-04 2006-11-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Assay with osmotically induced separation and enrichment of high molecular weight substances to be detected and fluidic microsystem for its implementation
EP2012923A1 (en) * 2006-04-07 2009-01-14 Corning Incorporated Closed flow-through microplate and methods for using and manufacturing same
DE102007011866A1 (en) 2007-03-08 2008-09-11 Friedrich-Schiller-Universität Jena Apparatus for receiving, treating and storing small-volume samples
WO2011009073A1 (en) * 2009-07-16 2011-01-20 Abbott Laboratories A method for amplification of nucleic acids
EP2825309B1 (en) 2012-03-16 2018-05-16 Stat-Diagnostica & Innovation, S.L. A test cartridge with integrated transfer module
EP3875574B1 (en) * 2018-10-29 2023-07-05 NOK Corporation Micro-fluid chip and micro-fluid device
WO2022167617A1 (en) * 2021-02-05 2022-08-11 Synhelix Improved thermocycled multistep reactions device

Citations (5)

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Publication number Priority date Publication date Assignee Title
WO1988004428A1 (en) * 1986-12-12 1988-06-16 Iq (Bio) Limited Device and method for biochemical assay
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
WO1996007917A1 (en) * 1994-09-09 1996-03-14 Nanogen, Inc. Automated molecular biological diagnostic system
WO2000044497A1 (en) * 1999-01-29 2000-08-03 Genomic Instrumentation Services, Inc. Pipetter
DE19950385A1 (en) * 1999-01-29 2000-08-03 Max Planck Gesellschaft Process for the isolation of apoptosis-inducing DNA sequences and detection system

Family Cites Families (5)

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US3591493A (en) * 1968-06-17 1971-07-06 Rashid A Zeineh Method for the treatment of biological fluids and apparatus therefor
US5010930A (en) * 1989-12-22 1991-04-30 Eastman Kodak Company Pipette and liquid transfer apparatus for dispensing liquid for analysis
US5733442A (en) * 1990-12-07 1998-03-31 Shukla; Ashok K. Microdialysis/Microelectrodialysis system
WO1994025876A1 (en) * 1993-04-29 1994-11-10 Danfoss A/S Device for analyzing a fluid medium
DE10005024C2 (en) * 2000-02-04 2002-09-26 Eppendorf Ag dialysis plug

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988004428A1 (en) * 1986-12-12 1988-06-16 Iq (Bio) Limited Device and method for biochemical assay
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
WO1996007917A1 (en) * 1994-09-09 1996-03-14 Nanogen, Inc. Automated molecular biological diagnostic system
WO2000044497A1 (en) * 1999-01-29 2000-08-03 Genomic Instrumentation Services, Inc. Pipetter
DE19950385A1 (en) * 1999-01-29 2000-08-03 Max Planck Gesellschaft Process for the isolation of apoptosis-inducing DNA sequences and detection system

Also Published As

Publication number Publication date
AU2001286756A1 (en) 2002-03-04
WO2002015949A9 (en) 2003-03-27
WO2002016652A3 (en) 2003-07-10
AU2001286784A1 (en) 2002-03-04
WO2002015949A2 (en) 2002-02-28
WO2002015949A8 (en) 2002-07-04
WO2002016652A2 (en) 2002-02-28

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