WO2002002735A1 - Top and bottom open centrifuge tube - Google Patents

Top and bottom open centrifuge tube Download PDF

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Publication number
WO2002002735A1
WO2002002735A1 PCT/KR2001/000659 KR0100659W WO0202735A1 WO 2002002735 A1 WO2002002735 A1 WO 2002002735A1 KR 0100659 W KR0100659 W KR 0100659W WO 0202735 A1 WO0202735 A1 WO 0202735A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
section
cells
transparent
ring
Prior art date
Application number
PCT/KR2001/000659
Other languages
French (fr)
Inventor
Soon Wook Lee
Original Assignee
Soon Wook Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Soon Wook Lee filed Critical Soon Wook Lee
Priority to AU2001252743A priority Critical patent/AU2001252743A1/en
Publication of WO2002002735A1 publication Critical patent/WO2002002735A1/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B04B7/04—Casings facilitating discharge
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones

Definitions

  • the present invention relates to a top and bottom open centrifuge tube for accelerating the separation process of a multitude of cells and bacteria and improving the accuracy of examination and also at the same time allows the simple separation between liquid and foreign substances possible within the premises of examination center or research center. More specifically, the present invention relates to a top and bottom open centrifuge tube for separating a multitude of cells and bacteria during the bacteria cultivation of cerebrospinal fluid, ascitic fluid and pleural fluid, or cancer examination, or bacteria cultivation examination using various types of cotton sticks especially when rotating the centrifugal separation tube in order to precipitate the cells and bacteria at the bottom of the tube and collecting them in a ring-shaped collection tube after dipping a cotton stick into the centrifugal separation tube where a transporting cultivation paper is located and then coating on a slide or cultivating the bacteria.
  • the conventional method of examining bacteria usually involves collecting examining bodies (cells, bacteria) using a cotton stick from the patient's affected part and inserting the cotton stick into a test tube where a transporting cultivation paper is located and transporting the test tube to an examination room.
  • a transporting cultivation paper is located and transporting the test tube to an examination room.
  • an accurate examination work becomes very difficult when stained to jell type cultivation paper or the examination body is left behind in the test tube.
  • cerebrospinal fluid, ascitic fluid and pleural fluid to test the existence of cancer cells or leukemia
  • the remaining liquid after a centrifugal separation was discarded to leave only the precipitates for collecting the cells and the precipitates were then coated on the slide. This process was time consuming and some of the cells were lost in the process.
  • the examination body container used in each of the hospital, pharmaceutical company, food research center and quarantine center has a lid on the top and the bottom part is sealed. As a result, some serious difficulties arise due to the problems as mentioned above during the cell separation.
  • FIG. 1 is a disassembled view of centrifugal cell separation transparent tube according to the present invention.
  • FIG. 2 is a cross section of assembled centrifugal cell separation transparent tube according to the present invention.
  • FIG. 3 shows the pumping tube section on the lid at the upper end of disassembled view of centrifugal cell separation transparent tube according to another embodiment of the present invention.
  • FIG. 4 is a disassembled view of centrifugal cell separation transparent tube according to further embodiment of the present invention.
  • FIG. 5 shows the cross section of a practical example of centrifugal cell separation transparent tube in FIG. 4.
  • FIG. 6 shows the pumping tube section on the lid at the upper end of disassembled view of centrifugal cell separation transparent tube according to another embodiment of FIG.
  • the present invention is designed to overcome the above problems of prior arts.
  • the present invention comprises a conventional lid fixed by a screw on the upper end of a transparent synthetic resin tube, a single body ring-shaped collection tube in a gourd shape on the lower end of the transparent synthetic resin tube and a bolt and a nut section at the tapered section on the upper section of the ring-shaped collection tube.
  • the bolt section is united with a support cap and a watertight cap is formed at the center section of the support cap in order to seal the entry of the ring-type collection tube.
  • the present invention allows a convenient and accurate examination within shorter examination time possible by being able to separate the cells without discarding the liquid at the time of cell separation. This is achieved by uniting with the support cap at the lower section of the transparent tube and inserting the liquid or inserting the collected cells to the liquid while the lid at the upper section open and allowing the cells accumulate within the ring-shaped collection tube through downward precipitation when the centrifugal separation is performed.
  • a tube with an opening/closing lid at the upper end of opening section comprising: a tapered section 5 in a funnel shape at the lower end of a synthetic resin body 2; a transparent tube 1 which forms a single body with a ring-shape collection tube in a gourd shape with an entry at the lower section; a transparent support cap 9, where a bolt section is formed at the connection between the lower end of said transparent tube 1 and the tapered section; a ring-shape collection tube 6 which forms a single body with a water-tight cap 8 that is located at the center of the transparent support cap 9 which in itself unites with the lower end of said transparent tube 1 and is designed to seal the entry 7 by unifying with the transparent tube 1; wherein the cells precipitate in the ring- shape collection tube 6 if the cells 13 or the bodily fluid on which the cells are floating are inserted to the liquid 12 in said transparent tube 1 and the cells can be separated by contracting the body of synthetic resin transparent tube 1 during the cell separation.
  • the upper section of the lid that unites with the upper end opening section of said transparent tube 1 is formed in a concave tube shape in order to be able to pump out during the cell separation.
  • the collected cells 13 from the patient's affected part are inserted to the liquid 12 contained in the transparent tube through the opening section at the upper end after sealing the entry 7 of the ring-shape collection tube 6 at the lower end by uniting with the transparent support cap at the lower end of the transparent tube 1.
  • the cells in the liquid are precipitated downward and collected in the ring-shaped collection tube 6 consequently forming a accumulation as shown in FIG. 2.
  • the cells 13 (examining body) collected in the ring-shaped collection tube 6 when coated on a slide cultivation paper only the cells can be separated and withdrawn if the body 12 of the synthetic resin transparent tube 1 is pressed slightly after opening the transparent support cap 9.
  • the lid that unites with the upper end opening section of said transparent tube 1 is formed in flexible synthetic resin or a tube in rubber material in order to be in a concave tube shape as shown in FIG. 3.
  • the concave tube part at the upper end of the lid 4' can be pumped in order to separate the cells instead of pressing the body 2 for pumping purpose.
  • a bolt section is formed at the connection between the lower end of the transparent tube 1 ' and the tapered section 5', in order to seal the watertight cap 8 and withdrawal tube 7' when united with the transparent support cap.
  • the transparent tube 1 ' in FIG. 4 when the examining body is inserted to the liquid the cells accumulate in the withdrawal tube T at the lower end of the funnel shaped tapered section therefore the cells accumulated in the withdrawal tube 7' can be withdrawn by pumping the lid 4' that has a concave tube at the upper end or at the body of the transparent tube 1'.
  • the present invention is convenient and simple for separating cells, bacteria and liquid but its use is not only limited for examining cancer cells. It can also be used for separating between testing sample and testing medicine using a centrifugal separator in a quarantine office for examining the farming products and livestock.
  • test tube and sampling tube are not necessary instead the present invention can perform these two functions.

Abstract

The present invention relates to a top-bottom opening and closing type centrifugal separation tube for accelerating the separation process of multitude of cells and bacteria and improving the accuracy of examination and also at the same time allows the simple separation between liquid and foreign substances possible. The present invention comprises a conventional lid fixed by a screw on the upper end of a transparent synthetic resin tube, a single body ring-tube shaped collection tube in a gourd shape on the lower end of the transparent synthetic resin tube and a bolt and a nut section at the tapered section on the upper section of the ring-shaped collection tube. The bolt section is united with a support cap and a watertight cap is formed at the center section of the support cap in order to seal the entry of the ring-type collection tube. The present invention allows a convenient and accurate examination within shorter examination time possible by being able to separate the cells without discarding the liquid at the time of cell separation. This is achieved by uniting with the support cap at the lower section of the transparent tube and inserting the liquid or inserting the collected cells to the liquid with the lid at the upper section open and allowing the cells accumulate within the ring-shaped collection tube through downward precipitation when centrifugal separation is performed.

Description

TOP AND BOTTOM OPEN CENTRIFUGE TUBE
BACKGROUND OF THE INVENTION
The present invention relates to a top and bottom open centrifuge tube for accelerating the separation process of a multitude of cells and bacteria and improving the accuracy of examination and also at the same time allows the simple separation between liquid and foreign substances possible within the premises of examination center or research center. More specifically, the present invention relates to a top and bottom open centrifuge tube for separating a multitude of cells and bacteria during the bacteria cultivation of cerebrospinal fluid, ascitic fluid and pleural fluid, or cancer examination, or bacteria cultivation examination using various types of cotton sticks especially when rotating the centrifugal separation tube in order to precipitate the cells and bacteria at the bottom of the tube and collecting them in a ring-shaped collection tube after dipping a cotton stick into the centrifugal separation tube where a transporting cultivation paper is located and then coating on a slide or cultivating the bacteria.
SUMMARY OF THE INVENTION
The conventional method of examining bacteria usually involves collecting examining bodies (cells, bacteria) using a cotton stick from the patient's affected part and inserting the cotton stick into a test tube where a transporting cultivation paper is located and transporting the test tube to an examination room. However, in this case, an accurate examination work becomes very difficult when stained to jell type cultivation paper or the examination body is left behind in the test tube. Also, in order to examine cerebrospinal fluid, ascitic fluid and pleural fluid to test the existence of cancer cells or leukemia, the remaining liquid after a centrifugal separation was discarded to leave only the precipitates for collecting the cells and the precipitates were then coated on the slide. This process was time consuming and some of the cells were lost in the process. Presently, the examination body container used in each of the hospital, pharmaceutical company, food research center and quarantine center has a lid on the top and the bottom part is sealed. As a result, some serious difficulties arise due to the problems as mentioned above during the cell separation.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a disassembled view of centrifugal cell separation transparent tube according to the present invention.
FIG. 2 is a cross section of assembled centrifugal cell separation transparent tube according to the present invention.
FIG. 3 shows the pumping tube section on the lid at the upper end of disassembled view of centrifugal cell separation transparent tube according to another embodiment of the present invention.
FIG. 4 is a disassembled view of centrifugal cell separation transparent tube according to further embodiment of the present invention.
FIG. 5 shows the cross section of a practical example of centrifugal cell separation transparent tube in FIG. 4.
FIG. 6 shows the pumping tube section on the lid at the upper end of disassembled view of centrifugal cell separation transparent tube according to another embodiment of FIG.
4.
(Description of the numeric on the main parts of the drawings) 1, 1': Transparent Tube
2: Body
3: Opening Section 4, 4': Lid
5, 5': Tapered Section
6: Ring-Shape Collection Tube
7: Entry 7': Withdrawal Tube
8: WaterTight Cap
9, 9': Transparent Support Cap
10: Bolt Section
11: Nut Section 12: Liquid
13: Cells or Bacteria within the Examination Body
DETAILED DESCRIPTION OF THE EMBODIMENTS
The present invention is designed to overcome the above problems of prior arts. The present invention comprises a conventional lid fixed by a screw on the upper end of a transparent synthetic resin tube, a single body ring-shaped collection tube in a gourd shape on the lower end of the transparent synthetic resin tube and a bolt and a nut section at the tapered section on the upper section of the ring-shaped collection tube.
The bolt section is united with a support cap and a watertight cap is formed at the center section of the support cap in order to seal the entry of the ring-type collection tube.
The present invention allows a convenient and accurate examination within shorter examination time possible by being able to separate the cells without discarding the liquid at the time of cell separation. This is achieved by uniting with the support cap at the lower section of the transparent tube and inserting the liquid or inserting the collected cells to the liquid while the lid at the upper section open and allowing the cells accumulate within the ring-shaped collection tube through downward precipitation when the centrifugal separation is performed.
Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
A tube with an opening/closing lid at the upper end of opening section comprising: a tapered section 5 in a funnel shape at the lower end of a synthetic resin body 2; a transparent tube 1 which forms a single body with a ring-shape collection tube in a gourd shape with an entry at the lower section; a transparent support cap 9, where a bolt section is formed at the connection between the lower end of said transparent tube 1 and the tapered section; a ring-shape collection tube 6 which forms a single body with a water-tight cap 8 that is located at the center of the transparent support cap 9 which in itself unites with the lower end of said transparent tube 1 and is designed to seal the entry 7 by unifying with the transparent tube 1; wherein the cells precipitate in the ring- shape collection tube 6 if the cells 13 or the bodily fluid on which the cells are floating are inserted to the liquid 12 in said transparent tube 1 and the cells can be separated by contracting the body of synthetic resin transparent tube 1 during the cell separation.
According to another embodiment of the present invention, the upper section of the lid that unites with the upper end opening section of said transparent tube 1 is formed in a concave tube shape in order to be able to pump out during the cell separation.
According to a further embodiment of the present invention, in order to be able to separate the cells a funnel shape tapered section 5' and a withdrawal tube 7' at the lower section of the transparent tube 1 ' that has a concave shaped lid 4' at the upper section or a conventional lid 4 at the upper end of the opening section 3 and a transparent support cap 9' that has a water-tight cap 8 by forming a bolt at the connection between the lower end of the transparent tube 1 ' and the tapered section.
According to the present invention, the collected cells 13 from the patient's affected part are inserted to the liquid 12 contained in the transparent tube through the opening section at the upper end after sealing the entry 7 of the ring-shape collection tube 6 at the lower end by uniting with the transparent support cap at the lower end of the transparent tube 1. As mentioned above, after inserting the cells or the bodily fluid on which the cells are floating are inserted to the liquid 12 and when the transparent tube is rotated using a centrifugal separator after closing the lid 4 at the opening section 3, the cells in the liquid are precipitated downward and collected in the ring-shaped collection tube 6 consequently forming a accumulation as shown in FIG. 2. The cells 13 (examining body) collected in the ring-shaped collection tube 6 when coated on a slide cultivation paper, only the cells can be separated and withdrawn if the body 12 of the synthetic resin transparent tube 1 is pressed slightly after opening the transparent support cap 9.
At this instance, even if the transparent support cap 9 is opened, the liquid or the cells do not flow out since the lid 4 at the upper end is closed.
As mentioned above, during the separation of the cells in the ring-shaped collection tube 6 towards the entry 7 by pressing the body 2 of the transparent tube 1, the liquid is not completely sealed but small amount of the liquid flows out. However, a much greater amount of the cells pass through the entry 7 than the liquid. According to another embodiment of the present invention, the lid that unites with the upper end opening section of said transparent tube 1 is formed in flexible synthetic resin or a tube in rubber material in order to be in a concave tube shape as shown in FIG. 3. Hence, during the separation of the cells accumulated in the ring-shaped collection tube 6, the concave tube part at the upper end of the lid 4' can be pumped in order to separate the cells instead of pressing the body 2 for pumping purpose.
According to still another embodiment of the present invention, as shown in FIG. 4 and FIG. 5, a funnel shape tapered section 5' and a withdrawal tube 7' at the lower section of the transparent tube 1 ' that has a concave shaped lid 4' at the upper section or a conventional lid 4 at the upper end of the opening section 3 in FIG. 6 in order to make the cells to accumulate in the tapered section 5' and withdrawal tube 7'. A bolt section is formed at the connection between the lower end of the transparent tube 1 ' and the tapered section 5', in order to seal the watertight cap 8 and withdrawal tube 7' when united with the transparent support cap.
Likewise, the transparent tube 1 ' in FIG. 4 when the examining body is inserted to the liquid, the cells accumulate in the withdrawal tube T at the lower end of the funnel shaped tapered section therefore the cells accumulated in the withdrawal tube 7' can be withdrawn by pumping the lid 4' that has a concave tube at the upper end or at the body of the transparent tube 1'.
The present invention is convenient and simple for separating cells, bacteria and liquid but its use is not only limited for examining cancer cells. It can also be used for separating between testing sample and testing medicine using a centrifugal separator in a quarantine office for examining the farming products and livestock.
Also, if the present invention is to be used by students in science laboratory, the test tube and sampling tube are not necessary instead the present invention can perform these two functions.

Claims

WHAT IS CLAIMED IS:
1. A top and bottom open centrifuge tube comprising: a tapered section 5 in a funnel shape at the lower end of a synthetic resin body 2; a transparent tube 1 which forms a single body with a ring-shape collection tube in a gourd shape with an entry at the lower section; a transparent support cap 9, where a bolt section is formed at the connection between the lower end of said transparent tube 1 and the tapered section; and a ring-shape collection tube 6 which forms a single body with a water-tight cap 8 that is located at the center of the transparent support cap 9 which in itself unites with the lower end of said transparent tube 1 and is designed to seal the entry 7 by unifying with the transparent tube 1; wherein the cells precipitate in the ring-shape collection tube 6 if the cells 13 or the bodily fluid on which the cells are floating are inserted to the liquid 12 in said transparent tube 1 and the cells can be separated by contracting the body of synthetic resin transparent tube 1 during the cell separation.
2. The tube as claimed in claim 1, wherein the upper section of the lid that unites with the upper end opening section of said transparent tube 1 is formed in a concave tube shape in order to be able to pump out during the cell separation.
3. The tube as claimed in claim 1, wherein said upper end of the opening section unites with a conventional lid 4 and a funnel shape tapered section 5' and a withdrawal tube 7' at the lower section of the transparent tube 1 ' are formed in order to make the cells inside to accumulate in the withdrawal tube 7' and a bolt section is formed at the connection between the lower end of the transparent tube 1' and the tapered section 5', in order to unite with the transparent support cap 9' that has a water-tight cap 8
PCT/KR2001/000659 2000-06-21 2001-04-20 Top and bottom open centrifuge tube WO2002002735A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001252743A AU2001252743A1 (en) 2000-06-21 2001-04-20 Top and bottom open centrifuge tube

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020000034212A KR100354341B1 (en) 2000-06-21 2000-06-21 Top and bottom open centrifuge tube
KR2000/34212 2000-06-21

Publications (1)

Publication Number Publication Date
WO2002002735A1 true WO2002002735A1 (en) 2002-01-10

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AU (1) AU2001252743A1 (en)
WO (1) WO2002002735A1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6866826B2 (en) * 2000-12-30 2005-03-15 Beckman Coulter, Inc. Large mouth centrifuge labware
CN102844121A (en) * 2009-12-19 2012-12-26 全玟墉 Centrifuge tube
CN103212125A (en) * 2013-04-01 2013-07-24 浙江康慈医疗科技有限公司 Method and special equipment for rapidly extracting platelet-rich high-concentration blood plasma
CN106442091A (en) * 2016-10-31 2017-02-22 上海化工研究院 Device for enriching trace volatile organic matters in water-containing solid on basis of hot-gas blow-off methods
GB2574046A (en) * 2018-05-24 2019-11-27 Oxford Nanopore Tech Ltd Improved container

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KR100749439B1 (en) * 2006-07-28 2007-08-14 임성오 Rotating bowl for centrifuge
KR100890316B1 (en) * 2007-06-25 2009-03-26 한국원자력연구원 Tube for Centrifuge
EP2511011A4 (en) * 2009-12-07 2014-07-09 Min-Yong Jeon Centrifuge tube
KR101188660B1 (en) 2010-07-08 2012-10-08 주식회사 무한기업 Test tube used in the centrifugal separator
CN103543057B (en) * 2013-11-13 2015-12-02 何鑫 The Combined centrifuge tube of cytology wax stone is prepared for micro-cast-off cells
KR101594539B1 (en) * 2014-02-17 2016-02-16 한국기초과학지원연구원 Concentration tube for aggregating motile cells
CN106140494A (en) * 2015-04-02 2016-11-23 马铁军 A kind of centrifugal when use special bottom opening centrifuge tube device
CN104990780B (en) * 2015-07-26 2017-10-17 赵稳兴 A kind of method for making cell block
KR101848869B1 (en) 2016-08-19 2018-04-13 (주) 굿모닝 바이오 Container for centrifugal separation
KR102274063B1 (en) * 2021-02-15 2021-07-08 최성민 QuEChERS kit for preprocessing of sample and preprocessing method using of that

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Publication number Priority date Publication date Assignee Title
US4364903A (en) * 1981-04-02 1982-12-21 Becton, Dickinson And Company Contamination-free separation device
KR880004313A (en) * 1986-12-24 1988-06-03 전동명 Urine tube device and method of separation
JPH1164183A (en) * 1997-08-07 1999-03-05 Bokei Cho Tube to automatically hold urine precipitate for quantitative centrifugal separation for urine analysis
US5996811A (en) * 1997-02-13 1999-12-07 Fuji Photo Film Co. Ltd. Plasma collecting device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4364903A (en) * 1981-04-02 1982-12-21 Becton, Dickinson And Company Contamination-free separation device
KR880004313A (en) * 1986-12-24 1988-06-03 전동명 Urine tube device and method of separation
US5996811A (en) * 1997-02-13 1999-12-07 Fuji Photo Film Co. Ltd. Plasma collecting device
JPH1164183A (en) * 1997-08-07 1999-03-05 Bokei Cho Tube to automatically hold urine precipitate for quantitative centrifugal separation for urine analysis

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6866826B2 (en) * 2000-12-30 2005-03-15 Beckman Coulter, Inc. Large mouth centrifuge labware
CN102844121A (en) * 2009-12-19 2012-12-26 全玟墉 Centrifuge tube
CN103212125A (en) * 2013-04-01 2013-07-24 浙江康慈医疗科技有限公司 Method and special equipment for rapidly extracting platelet-rich high-concentration blood plasma
CN106442091A (en) * 2016-10-31 2017-02-22 上海化工研究院 Device for enriching trace volatile organic matters in water-containing solid on basis of hot-gas blow-off methods
GB2574046A (en) * 2018-05-24 2019-11-27 Oxford Nanopore Tech Ltd Improved container
GB2574046B (en) * 2018-05-24 2022-12-07 Oxford Nanopore Tech Plc Improved Container

Also Published As

Publication number Publication date
KR20010114105A (en) 2001-12-29
AU2001252743A1 (en) 2002-01-14
KR100354341B1 (en) 2002-09-30

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