WO2002000729A3 - Generation of libraries of antibodies in yeast and uses thereof - Google Patents
Generation of libraries of antibodies in yeast and uses thereof Download PDFInfo
- Publication number
- WO2002000729A3 WO2002000729A3 PCT/US2001/020542 US0120542W WO0200729A3 WO 2002000729 A3 WO2002000729 A3 WO 2002000729A3 US 0120542 W US0120542 W US 0120542W WO 0200729 A3 WO0200729 A3 WO 0200729A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tester
- proteins
- protein
- target
- library
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01950579A EP1297124A2 (en) | 2000-06-23 | 2001-06-25 | Generation of libraries of antibodies in yeast and uses thereof |
AU2001271553A AU2001271553A1 (en) | 2000-06-23 | 2001-06-25 | High throughput generation and screening of fully human antibody repertoire in yeast |
CA002411515A CA2411515A1 (en) | 2000-06-23 | 2001-06-25 | Generation of libraries of antibodies in yeast and uses thereof |
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60297300A | 2000-06-23 | 2000-06-23 | |
US60297200A | 2000-06-23 | 2000-06-23 | |
US09/603,658 US6410246B1 (en) | 2000-06-23 | 2000-06-23 | Highly diverse library of yeast expression vectors |
US09/603,663 | 2000-06-23 | ||
US09/603,658 | 2000-06-23 | ||
US09/603,663 US6406863B1 (en) | 2000-06-23 | 2000-06-23 | High throughput generation and screening of fully human antibody repertoire in yeast |
US09/602,373 US6410271B1 (en) | 2000-06-23 | 2000-06-23 | Generation of highly diverse library of expression vectors via homologous recombination in yeast |
US09/602,972 | 2000-06-23 | ||
US09/602,373 | 2000-06-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002000729A2 WO2002000729A2 (en) | 2002-01-03 |
WO2002000729A3 true WO2002000729A3 (en) | 2002-06-13 |
Family
ID=24411088
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/020542 WO2002000729A2 (en) | 2000-06-23 | 2001-06-25 | Generation of libraries of antibodies in yeast and uses thereof |
Country Status (2)
Country | Link |
---|---|
US (2) | US6410271B1 (en) |
WO (1) | WO2002000729A2 (en) |
Families Citing this family (51)
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US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
US6759243B2 (en) * | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
US6610472B1 (en) * | 2000-10-31 | 2003-08-26 | Genetastix Corporation | Assembly and screening of highly complex and fully human antibody repertoire in yeast |
US7704964B2 (en) | 2001-08-17 | 2010-04-27 | Exothera L.L.C. | Methods and compounds for the targeting of protein to exosomes |
US20030059888A1 (en) * | 2001-08-22 | 2003-03-27 | Shengfeng Li | Compositions and methods for generating antigen-binding units |
EP1438400B1 (en) * | 2001-10-01 | 2009-06-17 | Dyax Corp. | Multi-chain eukaryotic display vectors and uses thereof |
US20050075298A1 (en) * | 2002-01-31 | 2005-04-07 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the intestinal mucosa |
US20040009937A1 (en) * | 2002-01-31 | 2004-01-15 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the respiratory system |
US20040043003A1 (en) * | 2002-01-31 | 2004-03-04 | Wei Chen | Clinical grade vectors based on natural microflora for use in delivering therapeutic compositions |
US8853362B2 (en) | 2002-05-22 | 2014-10-07 | Esbatech, An Alcon Biomedical Research Unit Llc | Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same |
EP1932916A1 (en) * | 2002-06-14 | 2008-06-18 | Dyax Corporation | Methods involving recombination of nucleic acid library members |
ATE386810T1 (en) * | 2002-06-14 | 2008-03-15 | Dyax Corp | RECOMBINATION OF NUCLEIC ACID LIBRARY MEMBERS |
JP4748984B2 (en) * | 2002-06-16 | 2011-08-17 | ダイアックス、コープ | Recombination of nucleic acid library members |
US20040067532A1 (en) | 2002-08-12 | 2004-04-08 | Genetastix Corporation | High throughput generation and affinity maturation of humanized antibody |
JP4939926B2 (en) | 2003-02-14 | 2012-05-30 | アノシス・インコーポレーテッド | Methods and compounds for generating antibodies and screening antibody repertoires |
EP1613751A1 (en) | 2003-04-11 | 2006-01-11 | Esbatech AG | Method for the construction of randomized gene sequence libraries in cells |
US7709610B2 (en) | 2003-05-08 | 2010-05-04 | Facet Biotech Corporation | Therapeutic use of anti-CS1 antibodies |
US20050025763A1 (en) | 2003-05-08 | 2005-02-03 | Protein Design Laboratories, Inc. | Therapeutic use of anti-CS1 antibodies |
CA2525250A1 (en) * | 2003-05-09 | 2004-11-25 | Health Research Inc. | Improved methods for protein interaction determination |
US7973134B2 (en) | 2004-07-07 | 2011-07-05 | Cell Signaling Technology, Inc. | Reagents for the detection of protein phosphorylation in anaplastic large cell lymphoma signaling pathways |
US7935790B2 (en) | 2004-10-04 | 2011-05-03 | Cell Singaling Technology, Inc. | Reagents for the detection of protein phosphorylation in T-cell receptor signaling pathways |
US7807789B2 (en) | 2004-12-21 | 2010-10-05 | Cell Signaling Technology, Inc. | Reagents for the detection of protein phosphorylation in EGFR-signaling pathways |
WO2007027906A2 (en) | 2005-08-31 | 2007-03-08 | Cell Signaling Technology, Inc. | Reagents for the detection of protein phosphorylation in leukemia signaling pathways |
US7745393B2 (en) * | 2005-11-03 | 2010-06-29 | Jumi Shin | Minimalist bZIP proteins and uses thereof |
EP1951890A4 (en) | 2005-11-16 | 2009-06-24 | Ambrx Inc | Methods and compositions comprising non-natural amino acids |
JP2009520468A (en) | 2005-12-23 | 2009-05-28 | ヴィヴェンティア バイオテック インコーポレーティッド | Fusion protein library production and screening methods, and uses thereof |
US7939636B2 (en) | 2006-08-11 | 2011-05-10 | Cell Signaling Technology, Inc. | Reagents for the detection of protein phosphorylation in c-Src signaling pathways |
FR2910493B1 (en) * | 2006-12-20 | 2012-12-14 | Bio Modeling Systems Ou Bmsystems | METHOD FOR THE RANDOM DIVERSIFICATION OF A GENETIC SEQUENCE FOR PRESERVING THE IDENTITY OF CERTAIN INTERNAL SEGMENTS OF THE GENETIC SEQUENCE |
US20090081659A1 (en) | 2007-03-07 | 2009-03-26 | Cell Signaling Technology, Inc. | Reagents for the detection of protein phosphorylation in carcinoma signaling pathways |
EP1975184A3 (en) | 2007-03-26 | 2008-11-26 | Cell Signaling Technology, Inc. | Serine or threonine phosphorylation sites |
EP1983002A3 (en) | 2007-04-19 | 2009-03-11 | Peter Hornbeck | Tyrosine phosphorylation sites and antibodies specific for them |
US7977462B2 (en) | 2007-04-19 | 2011-07-12 | Cell Signaling Technology, Inc. | Tyrosine phosphorylation sites |
EP1983003A3 (en) | 2007-04-19 | 2009-03-11 | Peter Hornbeck | Tyrosine phosphorylation sites and antibodies specific for them |
US20090053831A1 (en) | 2007-05-01 | 2009-02-26 | Cell Signaling Technology, Inc. | Tyrosine phosphorylation sites |
EP2193146B1 (en) | 2007-09-14 | 2016-05-25 | Adimab, LLC | Rationally designed, synthetic antibody libraries and uses therefor |
US8877688B2 (en) | 2007-09-14 | 2014-11-04 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
EP2062920A3 (en) | 2007-11-21 | 2009-06-17 | Peter Hornbeck | Protein phosphorylation by basophilic serine/threonine kinases in insulin signalling pathways |
US20110070601A1 (en) * | 2008-01-23 | 2011-03-24 | Rigshospitalet | Classification of individuals suffering from cardiovascular diseases according to survival prognoses as found by measuring the levels of biomarker ykl-40 |
EP2274437B1 (en) | 2008-04-10 | 2015-12-23 | Cell Signaling Technology, Inc. | Compositions and methods for detecting egfr mutations in cancer |
CN101343323B (en) * | 2008-08-29 | 2011-02-16 | 浙江大学 | Anti-interleukins-8 antibody |
EP4219805A1 (en) | 2010-07-16 | 2023-08-02 | Adimab, LLC | Antibody libraries |
US9915665B2 (en) | 2012-12-28 | 2018-03-13 | Abbvie Inc. | High-throughput system and method for identifying antibodies having specific antigen binding activities |
US9458244B2 (en) | 2012-12-28 | 2016-10-04 | Abbvie Inc. | Single chain multivalent binding protein compositions and methods |
EP3564379A1 (en) | 2013-09-13 | 2019-11-06 | California Institute of Technology | Selective recovery |
EP3077508B1 (en) | 2013-12-04 | 2018-11-14 | AxioMx, Inc. | Methods of utilizing recombination for the identification of binding moieties |
US10816554B2 (en) | 2014-04-04 | 2020-10-27 | The Board Of Trustees Of The Leland Stanford Junior University | Ligand discovery for T cell receptors |
SG11201804713VA (en) | 2015-12-11 | 2018-07-30 | California Inst Of Techn | TARGETING PEPTIDES FOR DIRECTING ADENO-ASSOCIATED VIRUSES (AAVs) |
AU2018359218B2 (en) | 2017-11-01 | 2021-11-11 | Nantbio, Inc. | IL8 blocking EMT pathway and overcoming cancer stem cells |
US20200370070A1 (en) * | 2017-11-17 | 2020-11-26 | The Johns Hopkins University | Compositions and methods for efficient genome editing |
WO2020068990A1 (en) | 2018-09-26 | 2020-04-02 | California Institute Of Technology | Adeno-associated virus compositions for targeted gene therapy |
CN113215145B (en) * | 2021-05-08 | 2022-07-08 | 河南大学 | Short fragment cloning method independent of PCR reaction |
Citations (1)
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WO1999028502A1 (en) * | 1997-11-28 | 1999-06-10 | Invitrogen Corporation | Single chain monoclonal antibody fusion reagents that regulate transcription in vivo |
Family Cites Families (15)
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US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
US5283173A (en) | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
US5962255A (en) * | 1992-03-24 | 1999-10-05 | Cambridge Antibody Technology Limited | Methods for producing recombinant vectors |
DE69334095T2 (en) | 1992-07-17 | 2007-04-12 | Dana-Farber Cancer Institute, Boston | Method for intracellular binding of targeted molecules |
US5922545A (en) | 1993-10-29 | 1999-07-13 | Affymax Technologies N.V. | In vitro peptide and antibody display libraries |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5525490A (en) | 1994-03-29 | 1996-06-11 | Onyx Pharmaceuticals, Inc. | Reverse two-hybrid method |
US5888773A (en) | 1994-08-17 | 1999-03-30 | The United States Of America As Represented By The Department Of Health And Human Services | Method of producing single-chain Fv molecules |
US5763733A (en) | 1994-10-13 | 1998-06-09 | Enzon, Inc. | Antigen-binding fusion proteins |
GB9504344D0 (en) | 1995-03-03 | 1995-04-19 | Unilever Plc | Antibody fragment production |
WO1997041255A1 (en) | 1996-04-26 | 1997-11-06 | Massachusetts Institute Of Technology | Three-hybrid screening assay |
US5955275A (en) | 1997-02-14 | 1999-09-21 | Arcaris, Inc. | Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes |
US5869250A (en) | 1996-12-02 | 1999-02-09 | The University Of North Carolina At Chapel Hill | Method for the identification of peptides that recognize specific DNA sequences |
AU7266898A (en) * | 1997-04-30 | 1998-11-24 | Enzon, Inc. | Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof |
US20020029391A1 (en) | 1998-04-15 | 2002-03-07 | Claude Geoffrey Davis | Epitope-driven human antibody production and gene expression profiling |
-
2000
- 2000-06-23 US US09/602,373 patent/US6410271B1/en not_active Expired - Lifetime
-
2001
- 2001-06-25 WO PCT/US2001/020542 patent/WO2002000729A2/en not_active Application Discontinuation
-
2002
- 2002-03-27 US US10/112,691 patent/US20030165990A1/en not_active Abandoned
Patent Citations (1)
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WO1999028502A1 (en) * | 1997-11-28 | 1999-06-10 | Invitrogen Corporation | Single chain monoclonal antibody fusion reagents that regulate transcription in vivo |
Non-Patent Citations (4)
Title |
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BENDIXEN C ET AL: "A yeast mating selection scheme for detection of protein-protein interaction", NUCLEIC ACIDS RES., vol. 22, no. 9, 1994, pages 1778 - 1779, XP002004038 * |
HOLTZ, A. E. ET AL.: "Yeast Hybrid Technologies: Chapter 5 (Mating-Based Yeast Two-Hybrid Screening)", 2000, EATON PUBLISHING, NATICK, MA, XP001065214 * |
VISINTIN MICHELA ET AL: "Selection of antibodies for intracellular function using a two-hybrid in vivo system", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 96, no. 21, 12 October 1999 (1999-10-12), pages 11723 - 11728, XP002143442, ISSN: 0027-8424 * |
WANG PETER L: "Creating hybrid genes by homologous recombination.", DISEASE MARKERS, vol. 16, no. 1-2, 2000, Meeting on New Horizons for Phage Antibody Display Technology;Hull, England, UK; April 11-13, 2000, pages 3 - 13, XP001064283, ISSN: 0278-0240 * |
Also Published As
Publication number | Publication date |
---|---|
US6410271B1 (en) | 2002-06-25 |
US20030165990A1 (en) | 2003-09-04 |
WO2002000729A2 (en) | 2002-01-03 |
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