WO2001098338A3 - Fluorescent proteins - Google Patents

Fluorescent proteins Download PDF

Info

Publication number
WO2001098338A3
WO2001098338A3 PCT/EP2001/006848 EP0106848W WO0198338A3 WO 2001098338 A3 WO2001098338 A3 WO 2001098338A3 EP 0106848 W EP0106848 W EP 0106848W WO 0198338 A3 WO0198338 A3 WO 0198338A3
Authority
WO
WIPO (PCT)
Prior art keywords
gfp
gfps
mutation
fluorescent proteins
making
Prior art date
Application number
PCT/EP2001/006848
Other languages
French (fr)
Other versions
WO2001098338A2 (en
Inventor
Sara P Bjorn
Len Pagliaro
Ole Thastrup
Original Assignee
Bioimage As
Sara P Bjorn
Len Pagliaro
Ole Thastrup
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioimage As, Sara P Bjorn, Len Pagliaro, Ole Thastrup filed Critical Bioimage As
Priority to IL15311301A priority Critical patent/IL153113A0/en
Priority to DE60139556T priority patent/DE60139556D1/en
Priority to AU2001279669A priority patent/AU2001279669B2/en
Priority to AU7966901A priority patent/AU7966901A/en
Priority to EP01957861A priority patent/EP1299414B1/en
Priority to JP2002504293A priority patent/JP2005518180A/en
Priority to US10/296,953 priority patent/US20040072995A1/en
Priority to AT01957861T priority patent/ATE439373T1/en
Priority to CA2410413A priority patent/CA2410413C/en
Priority to DK01957861T priority patent/DK1299414T3/en
Publication of WO2001098338A2 publication Critical patent/WO2001098338A2/en
Publication of WO2001098338A3 publication Critical patent/WO2001098338A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.
PCT/EP2001/006848 2000-06-19 2001-06-18 Fluorescent proteins WO2001098338A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
IL15311301A IL153113A0 (en) 2000-06-19 2001-06-18 Fluorescent proteins
DE60139556T DE60139556D1 (en) 2000-06-19 2001-06-18 FLUORESCENT PROTEINS
AU2001279669A AU2001279669B2 (en) 2000-06-19 2001-06-18 Fluorescent proteins
AU7966901A AU7966901A (en) 2000-06-19 2001-06-18 Novel fluorescent proteins
EP01957861A EP1299414B1 (en) 2000-06-19 2001-06-18 Fluorescent proteins
JP2002504293A JP2005518180A (en) 2000-06-19 2001-06-18 Novel fluorescent protein
US10/296,953 US20040072995A1 (en) 2000-06-19 2001-06-18 Novel fluorescent proteins
AT01957861T ATE439373T1 (en) 2000-06-19 2001-06-18 FLUORESCENT PROTEINS
CA2410413A CA2410413C (en) 2000-06-19 2001-06-18 Fluorescent proteins
DK01957861T DK1299414T3 (en) 2000-06-19 2001-06-18 Fluorescent proteins

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DKPA200000953 2000-06-19
DKPA200000953 2000-06-19
US21268100P 2000-06-20 2000-06-20
US60/212,681 2000-06-20
US29017001P 2001-05-10 2001-05-10
US60/290,170 2001-05-10
DKPA200100739 2001-05-10
DKPA200100739 2001-05-10

Publications (2)

Publication Number Publication Date
WO2001098338A2 WO2001098338A2 (en) 2001-12-27
WO2001098338A3 true WO2001098338A3 (en) 2002-05-10

Family

ID=27439814

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006848 WO2001098338A2 (en) 2000-06-19 2001-06-18 Fluorescent proteins

Country Status (11)

Country Link
US (2) US7001986B2 (en)
EP (1) EP1299414B1 (en)
JP (2) JP2005518180A (en)
AT (1) ATE439373T1 (en)
AU (2) AU2001279669B2 (en)
CA (1) CA2410413C (en)
DE (1) DE60139556D1 (en)
DK (1) DK1299414T3 (en)
ES (1) ES2331109T3 (en)
IL (1) IL153113A0 (en)
WO (1) WO2001098338A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021355A1 (en) 1996-11-15 1998-05-22 Life Technologies, Inc. Mutants of green fluorescent protein
GB0109858D0 (en) 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
JP3829252B2 (en) 2001-06-08 2006-10-04 独立行政法人理化学研究所 Fluorescent protein
US7271241B2 (en) 2002-04-24 2007-09-18 Los Alamos National Security, Llc Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby
ATE509946T1 (en) * 2002-11-12 2011-06-15 Zakrytoe Aktsionernoe Obschestvo S Evrogens FLUORESCENT PROTEINS AND CHROMOPROTEINS FROM NON-AEQUOREA HYDROZOA SPECIES AND METHOD FOR THE USE THEREOF
CN1886420B (en) 2003-10-24 2012-02-29 加利福尼亚大学 Self-assembling split-fluorescent protein systems
CA2606876C (en) * 2005-05-03 2018-07-10 Georgia State University Research Foundation, Inc. Fluorescent proteins and uses thereof
EP1785434A1 (en) 2005-11-11 2007-05-16 Ludwig-Maximilians-Universität München Targeting and tracing of antigens in living cells
EP2054514A4 (en) 2006-08-04 2009-11-04 Univ Georgia State Res Found Enzyme sensors, methods for preparing and using such sensors, and methods of detecting protease activity
JPWO2009020197A1 (en) * 2007-08-03 2010-11-04 国立大学法人北海道大学 Ultramarine fluorescent protein
ES2606706T3 (en) 2008-01-09 2017-03-27 Ludwig-Maximilians-Universitat München A double fluorescent hybrid (F2H) assay for direct visualization of protein interactions in living cells
EP2275442A1 (en) 2009-07-06 2011-01-19 Ludwig-Maximilians-Universität München Detection and vizualization of the cell cycle in living cells
CN101974554A (en) * 2010-09-26 2011-02-16 武汉大学 Construction method and application of mutant vector pEGFP-N1m
EP2685260A1 (en) 2012-07-09 2014-01-15 Ludwig-Maximilians-Universität München Direct and quantitative detection of targets in living cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0851874A1 (en) * 1995-09-22 1998-07-08 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491084A (en) 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995007463A1 (en) 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
US5610031A (en) 1993-10-27 1997-03-11 The General Hospital Corporation B1k chain of laminin and methods of use
WO1995021191A1 (en) 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US6090919A (en) 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
GB0109858D0 (en) 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0851874A1 (en) * 1995-09-22 1998-07-08 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BREJC KATJUSA ET AL: "Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 94, no. 6, 1997, 1997, pages 2306 - 2311, XP002186298, ISSN: 0027-8424 *
HEIM R ET AL: "WAVELENGTH MUTATIONS AND POSTTRANSLATIONAL AUTOXIDATION OF GREEN FLUORESCENT PROTEIN", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, 1 December 1994 (1994-12-01), pages 12501 - 12504, XP000574454, ISSN: 0027-8424 *
T-T YANG ET AL: "Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 24, no. 22, 1996, pages 4592 - 4593, XP002120084, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
CA2410413C (en) 2010-05-18
JP2007075122A (en) 2007-03-29
CA2410413A1 (en) 2001-12-27
US7476518B2 (en) 2009-01-13
EP1299414A2 (en) 2003-04-09
US20020177189A1 (en) 2002-11-28
IL153113A0 (en) 2003-06-24
JP4459944B2 (en) 2010-04-28
WO2001098338A2 (en) 2001-12-27
US7001986B2 (en) 2006-02-21
DE60139556D1 (en) 2009-09-24
US20060051843A1 (en) 2006-03-09
EP1299414B1 (en) 2009-08-12
AU7966901A (en) 2002-01-02
ES2331109T3 (en) 2009-12-22
JP2005518180A (en) 2005-06-23
DK1299414T3 (en) 2009-11-30
ATE439373T1 (en) 2009-08-15
AU2001279669B2 (en) 2006-02-02

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