WO2001075154A3 - Novel method for the parallel sequencing of a nucleic acid mixture on a surface - Google Patents

Novel method for the parallel sequencing of a nucleic acid mixture on a surface Download PDF

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Publication number
WO2001075154A3
WO2001075154A3 PCT/EP2001/003777 EP0103777W WO0175154A3 WO 2001075154 A3 WO2001075154 A3 WO 2001075154A3 EP 0103777 W EP0103777 W EP 0103777W WO 0175154 A3 WO0175154 A3 WO 0175154A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
nucleic acid
tertiary nucleic
tertiary
acid mixture
Prior art date
Application number
PCT/EP2001/003777
Other languages
German (de)
French (fr)
Other versions
WO2001075154A2 (en
Inventor
Achim Fischer
Original Assignee
Axaron Bioscience Ag
Achim Fischer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10016348A external-priority patent/DE10016348A1/en
Priority claimed from DE2000151564 external-priority patent/DE10051564A1/en
Application filed by Axaron Bioscience Ag, Achim Fischer filed Critical Axaron Bioscience Ag
Priority to AU2001254771A priority Critical patent/AU2001254771A1/en
Publication of WO2001075154A2 publication Critical patent/WO2001075154A2/en
Publication of WO2001075154A3 publication Critical patent/WO2001075154A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The invention relates to a method for the parallel sequencing of at least two nucleic acids that are contained in a nucleic acid mixture. According to said method, a surface is provided which has islands of nucleic acids, each of the same type, i.e. tertiary nucleic acids; - the tertiary nucleic acids are treated in such a way that the products are only connected to the surface by the 5' end of one strand of a double-strand; - the tertiary nucleic acids are cleaved by restriction endonucleases of the IIS type, in such a way that 3' overhanging ends or 5' overhanging ends are created; - one or more bases of the tertiary nucleic acids are determined; - linker molecules are connected by ligation to the free ends of the tertiary nucleic acid molecules, whereby the linker molecules have one or more recognition sites for restriction endonucleases of the IIS type; - the tertiary nucleic acids are cleaved once again by one or more restriction endonucleases of the IIS type, which recognize the sequence parts that have been introduced into the tertiary nucleic acids by the linker molecules in step (e); and - steps (d), (e) and (f) are repeated until the desired sequence information is obtained.
PCT/EP2001/003777 2000-04-03 2001-04-03 Novel method for the parallel sequencing of a nucleic acid mixture on a surface WO2001075154A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001254771A AU2001254771A1 (en) 2000-04-03 2001-04-03 Novel method for the parallel sequencing of a nucleic acid mixture on a surface

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10016348A DE10016348A1 (en) 2000-04-03 2000-04-03 Parallel sequencing of several nucleic acids, useful e.g. in gene expression analysis, using irreversibly immobilized amplification primers
DE10016348.3 2000-04-03
DE10051564.9 2000-10-18
DE2000151564 DE10051564A1 (en) 2000-10-18 2000-10-18 Parallel sequencing of nucleic acids in a mixture, useful e.g. for detecting mutations, comprises a series of single-base extension reactions using protected, labeled nucleotides

Publications (2)

Publication Number Publication Date
WO2001075154A2 WO2001075154A2 (en) 2001-10-11
WO2001075154A3 true WO2001075154A3 (en) 2003-01-03

Family

ID=26005149

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/003777 WO2001075154A2 (en) 2000-04-03 2001-04-03 Novel method for the parallel sequencing of a nucleic acid mixture on a surface

Country Status (2)

Country Link
AU (1) AU2001254771A1 (en)
WO (1) WO2001075154A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1716254B1 (en) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
DE102004049532A1 (en) * 2004-10-09 2006-04-13 Fischer, Achim, Dr. Sequencing of Nucleic Acids via Beach Displacement
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP2426214A1 (en) * 2010-09-01 2012-03-07 Koninklijke Philips Electronics N.V. Method for amplifying nucleic acids
CN116888276A (en) * 2020-12-31 2023-10-13 北京美康基因科学股份有限公司 Multiple PCR library construction method for high-throughput targeted sequencing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides

Also Published As

Publication number Publication date
WO2001075154A2 (en) 2001-10-11
AU2001254771A1 (en) 2001-10-15

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