WO2001073129A3 - Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology') - Google Patents

Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology') Download PDF

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Publication number
WO2001073129A3
WO2001073129A3 PCT/US2001/009587 US0109587W WO0173129A3 WO 2001073129 A3 WO2001073129 A3 WO 2001073129A3 US 0109587 W US0109587 W US 0109587W WO 0173129 A3 WO0173129 A3 WO 0173129A3
Authority
WO
WIPO (PCT)
Prior art keywords
loci
pcr
technology
immunoas
luminiscent
Prior art date
Application number
PCT/US2001/009587
Other languages
French (fr)
Other versions
WO2001073129A2 (en
Inventor
Reinhold B Pollner
Original Assignee
Dade Behring Marburg Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg Gmbh filed Critical Dade Behring Marburg Gmbh
Priority to EP01920753A priority Critical patent/EP1297178A2/en
Publication of WO2001073129A2 publication Critical patent/WO2001073129A2/en
Publication of WO2001073129A3 publication Critical patent/WO2001073129A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Abstract

The current invention relates to the use of luminescent oxygen channeling immunoassay ('LOCI') technology to monitor amplification reactions, especially polymerase chain reactions ('PCR'). More specifically, the current invention involves the use of LOCI to measure the kinetics of a PCR reaction in an all-in-one assay format in order to quantitatively and qualitatively detect a target polynucleotide.
PCT/US2001/009587 2000-03-27 2001-03-26 Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology') WO2001073129A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01920753A EP1297178A2 (en) 2000-03-27 2001-03-26 Real time monitoring of pcr using loci ("luminescent oxygen channeling immunoassay technology")

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/536,936 US6346384B1 (en) 2000-03-27 2000-03-27 Real-time monitoring of PCR using LOCI
US09/536,936 2000-03-27

Publications (2)

Publication Number Publication Date
WO2001073129A2 WO2001073129A2 (en) 2001-10-04
WO2001073129A3 true WO2001073129A3 (en) 2003-01-23

Family

ID=24140527

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009587 WO2001073129A2 (en) 2000-03-27 2001-03-26 Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology')

Country Status (3)

Country Link
US (1) US6346384B1 (en)
EP (1) EP1297178A2 (en)
WO (1) WO2001073129A2 (en)

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US7381370B2 (en) * 2003-07-18 2008-06-03 Dade Behring Inc. Automated multi-detector analyzer
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US7122799B2 (en) * 2003-12-18 2006-10-17 Palo Alto Research Center Incorporated LED or laser enabled real-time PCR system and spectrophotometer
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
US7939267B2 (en) * 2004-11-04 2011-05-10 Laboratory Corporation Of America Holdings Detection of activation of endothelial cells as surrogate marker for angiogenesis
US8008066B2 (en) 2005-03-10 2011-08-30 Gen-Probe Incorporated System for performing multi-formatted assays
US20090155894A1 (en) * 2005-10-17 2009-06-18 Soper Steven A Electrokinetic Thermal Cycler and Reactor
EP3167900B1 (en) 2006-03-29 2018-11-21 Merial Limited Vaccine against streptococci
WO2008127921A2 (en) * 2007-04-13 2008-10-23 Abbott Laboratories Primer and probe sequences for detecting chlamydia trachomatis
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US9347947B2 (en) * 2009-03-12 2016-05-24 Siemens Healthcare Diagnostics Inc. Immunoassays employing non-particulate chemiluminescent reagent
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
JP6468838B2 (en) 2011-05-19 2019-02-13 ラボラトリー コーポレイション オブ アメリカ ホールディングス Methods for determining the survival potential of cancer patients and for predicting the likelihood of metastasis in cancer patients
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Also Published As

Publication number Publication date
EP1297178A2 (en) 2003-04-02
US6346384B1 (en) 2002-02-12
WO2001073129A2 (en) 2001-10-04

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