WO2001073129A3 - Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology') - Google Patents
Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology') Download PDFInfo
- Publication number
- WO2001073129A3 WO2001073129A3 PCT/US2001/009587 US0109587W WO0173129A3 WO 2001073129 A3 WO2001073129 A3 WO 2001073129A3 US 0109587 W US0109587 W US 0109587W WO 0173129 A3 WO0173129 A3 WO 0173129A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- loci
- pcr
- technology
- immunoas
- luminiscent
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Abstract
The current invention relates to the use of luminescent oxygen channeling immunoassay ('LOCI') technology to monitor amplification reactions, especially polymerase chain reactions ('PCR'). More specifically, the current invention involves the use of LOCI to measure the kinetics of a PCR reaction in an all-in-one assay format in order to quantitatively and qualitatively detect a target polynucleotide.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01920753A EP1297178A2 (en) | 2000-03-27 | 2001-03-26 | Real time monitoring of pcr using loci ("luminescent oxygen channeling immunoassay technology") |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/536,936 | 2000-03-27 | ||
US09/536,936 US6346384B1 (en) | 2000-03-27 | 2000-03-27 | Real-time monitoring of PCR using LOCI |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2001073129A2 WO2001073129A2 (en) | 2001-10-04 |
WO2001073129A3 true WO2001073129A3 (en) | 2003-01-23 |
Family
ID=24140527
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/009587 WO2001073129A2 (en) | 2000-03-27 | 2001-03-26 | Real time monitoring of pcr using loci ('luminiscent oxygen channeling immunoas say technology') |
Country Status (3)
Country | Link |
---|---|
US (1) | US6346384B1 (en) |
EP (1) | EP1297178A2 (en) |
WO (1) | WO2001073129A2 (en) |
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US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
US7771929B2 (en) * | 2000-04-28 | 2010-08-10 | Monogram Biosciences, Inc. | Tag library compounds, compositions, kits and methods of use |
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US20030207300A1 (en) * | 2000-04-28 | 2003-11-06 | Matray Tracy J. | Multiplex analytical platform using molecular tags |
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AU2003222253A1 (en) * | 2002-03-05 | 2003-09-22 | Monogram Biosciences, Inc. | Multiplex analysis using membrane-bound sensitizers |
US7402397B2 (en) * | 2002-05-21 | 2008-07-22 | Monogram Biosciences, Inc. | Detecting and profiling molecular complexes |
US20040229299A1 (en) * | 2002-05-21 | 2004-11-18 | Badal M. Youssouf | Intracellular complexes as biomarkers |
US20040229293A1 (en) * | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | Surface receptor complexes as biomarkers |
US20040229380A1 (en) * | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | ErbB heterodimers as biomarkers |
US20040229294A1 (en) | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | ErbB surface receptor complexes as biomarkers |
US20040175765A1 (en) * | 2002-07-05 | 2004-09-09 | Sharat Singh | Cell-screening assay and composition |
US20040014043A1 (en) * | 2002-07-16 | 2004-01-22 | Emp Biotech Gmbh | Sensitizer-labeled analyte detection |
CA2490654C (en) | 2002-07-25 | 2012-02-07 | Aclara Biosciences, Inc. | Detecting receptor oligomerization |
JP2004261017A (en) * | 2003-02-27 | 2004-09-24 | Arkray Inc | Method for detecting chlamydia trachomatis and kit for the same |
BRPI0408928A (en) | 2003-04-01 | 2006-09-12 | Monogram Biosciences Inc | methods to determine a disease situation in a patient, an apoptotic situation of cells in a sample, and a cancer situation in a patient |
US7402398B2 (en) * | 2003-07-17 | 2008-07-22 | Monogram Biosciences, Inc. | Measuring receptor homodimerization |
US7381370B2 (en) * | 2003-07-18 | 2008-06-03 | Dade Behring Inc. | Automated multi-detector analyzer |
JP2007502417A (en) * | 2003-08-11 | 2007-02-08 | モノグラム バイオサイエンシズ,インコーポレーテッド | Detection and characterization of molecular complexes |
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US7122799B2 (en) * | 2003-12-18 | 2006-10-17 | Palo Alto Research Center Incorporated | LED or laser enabled real-time PCR system and spectrophotometer |
WO2005077125A2 (en) * | 2004-02-11 | 2005-08-25 | Applera Corporation | Methods and compositions for detecting nucleic acids |
US7939267B2 (en) * | 2004-11-04 | 2011-05-10 | Laboratory Corporation Of America Holdings | Detection of activation of endothelial cells as surrogate marker for angiogenesis |
US7932081B2 (en) * | 2005-03-10 | 2011-04-26 | Gen-Probe Incorporated | Signal measuring system for conducting real-time amplification assays |
US20090155894A1 (en) * | 2005-10-17 | 2009-06-18 | Soper Steven A | Electrokinetic Thermal Cycler and Reactor |
AU2007233252C1 (en) | 2006-03-29 | 2013-05-23 | Boehringer Ingelheim Animal Health USA Inc. | Vaccine against Streptococci |
EP2514838B1 (en) * | 2007-04-13 | 2017-12-06 | Abbott Molecular Inc. | Primer and probe sequences for detecting chlamydia trachomatis |
US10416162B2 (en) * | 2007-12-20 | 2019-09-17 | Monogram Biosciences, Inc. | Her2 diagnostic methods |
US9347947B2 (en) * | 2009-03-12 | 2016-05-24 | Siemens Healthcare Diagnostics Inc. | Immunoassays employing non-particulate chemiluminescent reagent |
US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
CN103403533B (en) | 2011-02-24 | 2017-02-15 | 简.探针公司 | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
SG194609A1 (en) | 2011-05-19 | 2013-12-30 | Lab Corp America Holdings | Methods for determining the likelihood of survival and for predicting likelihood of metastasis in cancer patients |
EP2981828A1 (en) | 2013-04-05 | 2016-02-10 | Laboratory Corporation of America Holdings | Systems and methods for facilitating diagnosis, prognosis and treatment of cancer based on detection of her3 activation |
GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
CA3001020A1 (en) * | 2015-11-23 | 2017-06-01 | Perkinelmer Health Sciences, Inc. | Hollow polymer fiber optic system for single analyte and multiplexed analyte detection |
GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
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WO1995013399A1 (en) * | 1993-11-12 | 1995-05-18 | The Public Health Research Institute Of The City Of New York, Inc. | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
WO1997023647A1 (en) * | 1995-12-22 | 1997-07-03 | Behringwerke Aktiengesellschaft | Homogeneous amplification and detection of nucleic acids |
WO1998004738A1 (en) * | 1996-07-26 | 1998-02-05 | Winger Edward E | Real time homogeneous nucleotide assay |
WO1999042615A1 (en) * | 1998-02-18 | 1999-08-26 | Dade Behring Inc. | Methods for determining amounts of nucleic acids |
WO1999042616A1 (en) * | 1998-02-20 | 1999-08-26 | Dade Behring Inc. | Methods for determining concentrations of nucleic acids |
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ATE350665T1 (en) * | 1991-05-22 | 2007-01-15 | Dade Behring Marburg Gmbh | HOMOGENEOUS TEST SYSTEM FOR DETERMINING AN ANALYTE |
US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
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AU679008B2 (en) | 1993-05-06 | 1997-06-19 | Chiron Diagnostics Corporation | Mixed luminescent conjugate test assays |
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-
2000
- 2000-03-27 US US09/536,936 patent/US6346384B1/en not_active Expired - Lifetime
-
2001
- 2001-03-26 WO PCT/US2001/009587 patent/WO2001073129A2/en active Application Filing
- 2001-03-26 EP EP01920753A patent/EP1297178A2/en not_active Withdrawn
Patent Citations (5)
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---|---|---|---|---|
WO1995013399A1 (en) * | 1993-11-12 | 1995-05-18 | The Public Health Research Institute Of The City Of New York, Inc. | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
WO1997023647A1 (en) * | 1995-12-22 | 1997-07-03 | Behringwerke Aktiengesellschaft | Homogeneous amplification and detection of nucleic acids |
WO1998004738A1 (en) * | 1996-07-26 | 1998-02-05 | Winger Edward E | Real time homogeneous nucleotide assay |
WO1999042615A1 (en) * | 1998-02-18 | 1999-08-26 | Dade Behring Inc. | Methods for determining amounts of nucleic acids |
WO1999042616A1 (en) * | 1998-02-20 | 1999-08-26 | Dade Behring Inc. | Methods for determining concentrations of nucleic acids |
Also Published As
Publication number | Publication date |
---|---|
US6346384B1 (en) | 2002-02-12 |
EP1297178A2 (en) | 2003-04-02 |
WO2001073129A2 (en) | 2001-10-04 |
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