WO2001069230A3 - Methods and apparatus for the location and concentration of polar analytes using an alternating electric field - Google Patents

Methods and apparatus for the location and concentration of polar analytes using an alternating electric field Download PDF

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Publication number
WO2001069230A3
WO2001069230A3 PCT/US2001/007576 US0107576W WO0169230A3 WO 2001069230 A3 WO2001069230 A3 WO 2001069230A3 US 0107576 W US0107576 W US 0107576W WO 0169230 A3 WO0169230 A3 WO 0169230A3
Authority
WO
WIPO (PCT)
Prior art keywords
electric field
alternating electric
concentration
location
methods
Prior art date
Application number
PCT/US2001/007576
Other languages
French (fr)
Other versions
WO2001069230A2 (en
Inventor
Zbigniew T Bryning
Theresa B Taylor
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Priority to JP2001568062A priority Critical patent/JP2003527601A/en
Priority to DE60108848T priority patent/DE60108848T2/en
Priority to CA002402189A priority patent/CA2402189A1/en
Priority to AT01918488T priority patent/ATE289065T1/en
Priority to EP01918488A priority patent/EP1261863B1/en
Priority to AU2001245558A priority patent/AU2001245558A1/en
Publication of WO2001069230A2 publication Critical patent/WO2001069230A2/en
Publication of WO2001069230A3 publication Critical patent/WO2001069230A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Abstract

A method is disclosed for effecting the concentration of a polar analyte in an alternating electric field. In the method, a relative translation of the polar analyte and an alternating electric field along a translation path is effected. A portion of the polar analyte is then trapped and concentrated in a concentration zone formed by the intersection of the translation path and the alternating electric field. Also disclosed are various devices for carrying out the foregoing method.
PCT/US2001/007576 2000-03-10 2001-03-08 Methods and apparatus for the location and concentration of polar analytes using an alternating electric field WO2001069230A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2001568062A JP2003527601A (en) 2000-03-10 2001-03-08 Method and apparatus for locating and concentrating polar analytes using an alternating electric field
DE60108848T DE60108848T2 (en) 2000-03-10 2001-03-08 METHOD AND DEVICE FOR THE LOCATION AND CONCENTRATION OF POLAR ANALYTES BY MEANS OF AN ELECTRIC CHANGE AREA
CA002402189A CA2402189A1 (en) 2000-03-10 2001-03-08 Methods and apparatus for the location and concentration of polar analytes using an alternating electric field
AT01918488T ATE289065T1 (en) 2000-03-10 2001-03-08 METHOD AND DEVICE FOR LOCATION AND CONCENTRATION OF POLAR ANALYTES USING AN ALTERNATING ELECTRICAL FIELD
EP01918488A EP1261863B1 (en) 2000-03-10 2001-03-08 Method and apparatus for the location and concentration of polar analytes using an alternating electric field
AU2001245558A AU2001245558A1 (en) 2000-03-10 2001-03-08 Methods and apparatus for the location and concentration of polar analytes usingan alternating electric field

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/522,638 2000-03-10
US09/522,638 US6537433B1 (en) 2000-03-10 2000-03-10 Methods and apparatus for the location and concentration of polar analytes using an alternating electric field

Publications (2)

Publication Number Publication Date
WO2001069230A2 WO2001069230A2 (en) 2001-09-20
WO2001069230A3 true WO2001069230A3 (en) 2002-06-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007576 WO2001069230A2 (en) 2000-03-10 2001-03-08 Methods and apparatus for the location and concentration of polar analytes using an alternating electric field

Country Status (8)

Country Link
US (4) US6537433B1 (en)
EP (1) EP1261863B1 (en)
JP (1) JP2003527601A (en)
AT (1) ATE289065T1 (en)
AU (1) AU2001245558A1 (en)
CA (1) CA2402189A1 (en)
DE (1) DE60108848T2 (en)
WO (1) WO2001069230A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537433B1 (en) * 2000-03-10 2003-03-25 Applera Corporation Methods and apparatus for the location and concentration of polar analytes using an alternating electric field
AU2001288249A1 (en) * 2000-08-14 2002-02-25 The Regents Of The University Of California Biosensors and methods for their use
US7147441B2 (en) * 2000-12-20 2006-12-12 Board Of Trustees Of The University Of Arkansas, N.A. Microfluidics and small volume mixing based on redox magnetohydrodynamics methods
US20020187503A1 (en) * 2001-05-02 2002-12-12 Michael Harrold Concentration and purification of analytes using electric fields
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
US6814859B2 (en) * 2002-02-13 2004-11-09 Nanostream, Inc. Frit material and bonding method for microfluidic separation devices
GB0215779D0 (en) * 2002-07-08 2002-08-14 Deltadot Ltd Material separation device
JP3956389B2 (en) 2002-12-11 2007-08-08 横河電機株式会社 Hybridization method and apparatus for hybridization
JP4128084B2 (en) * 2003-01-07 2008-07-30 クラスターテクノロジー株式会社 Sample characterization method and apparatus
WO2004083823A1 (en) * 2003-03-19 2004-09-30 Nec Corporation Microchip, sampling method, sample separating method, sample analyzing method, and sample recovering method
US7147764B2 (en) * 2003-03-28 2006-12-12 Applera Corporation Dual electrode injection of analyte into a capillary electrophoretic device
ATE412902T1 (en) * 2003-04-14 2008-11-15 Caliper Life Sciences Inc REDUCING MIGRATION SHIFT ASSAY INTERFERENCE
US20120077206A1 (en) 2003-07-12 2012-03-29 Accelr8 Technology Corporation Rapid Microbial Detection and Antimicrobial Susceptibility Testing
CA2956645A1 (en) 2003-07-12 2005-03-31 David A. Goldberg Sensitive and rapid biodetection
JP4453334B2 (en) * 2003-10-21 2010-04-21 ソニー株式会社 Hybridization apparatus and method
US7384791B2 (en) * 2004-01-21 2008-06-10 Hewlett-Packard Development Company, L.P. Method of analyzing blood
US7160425B2 (en) * 2004-03-25 2007-01-09 Hewlett-Packard Development Company, L.P. Cell transporter for a biodevice
US7390388B2 (en) * 2004-03-25 2008-06-24 Hewlett-Packard Development Company, L.P. Method of sorting cells on a biodevice
US7390387B2 (en) * 2004-03-25 2008-06-24 Hewlett-Packard Development Company, L.P. Method of sorting cells in series
US8057655B1 (en) * 2004-08-20 2011-11-15 The Board Of Trustees Of The Leland Stanford Junior University Sub-micron object control arrangement and approach therefor
JP4619728B2 (en) * 2004-09-02 2011-01-26 株式会社エンプラス Sample analyzer
CA2589976A1 (en) * 2004-12-16 2006-06-22 Accelr8 Technology Corporation Rapid microbial detection and antimicrobial susceptibility testing
JP4462051B2 (en) * 2005-01-28 2010-05-12 富士ゼロックス株式会社 Concentration method for fine particle dispersion and concentration device for fine particle dispersion
ES2558797T3 (en) * 2005-10-04 2016-02-08 Headway Technologies, Inc. Microfluidic analyte detection
EP1984723B1 (en) * 2006-02-01 2019-05-15 Ecole Polytechnique Federale de Lausanne (EPFL) Impedance measurement device for characterizing particles in a micro channel
US8029657B1 (en) * 2006-03-14 2011-10-04 University Of Tennessee Research Foundation Parallel plate electrodes for particle concentration or removal
US7731826B2 (en) * 2006-08-17 2010-06-08 Electronic Bio Sciences, Llc Controlled translocation of a polymer in an electrolytic sensing system
US8293086B2 (en) * 2007-02-06 2012-10-23 Board Of Trustees Of Michigan State University Classification scheme for nanoobjects
WO2008124706A2 (en) 2007-04-06 2008-10-16 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Devices and methods for target molecule characterization
JP2010533840A (en) * 2007-07-13 2010-10-28 ザ ボード オブ トラスティーズ オブ ザ リランド スタンフォード ジュニア ユニヴァーシティ Methods and apparatus using electric fields for improved biological assays
EP2183202A2 (en) * 2007-07-20 2010-05-12 Siemens Medical Solutions USA, Inc. Microfluidic radiosynthesis of a radiolabeled compound using electrochemical trapping and release
US7655124B2 (en) * 2007-10-05 2010-02-02 Mady Attila Apparatus to assist platelet manipulation to prevent and treat endovascular disease and its sequelae
JP5120968B2 (en) * 2007-10-05 2013-01-16 国立大学法人九州工業大学 Dielectrophoresis apparatus and method
GB0804491D0 (en) * 2008-03-11 2008-04-16 Iti Scotland Ltd Detecting analytes
WO2010042514A1 (en) 2008-10-06 2010-04-15 Arizona Board Of Regents Nanopore and carbon nanotube based dna sequencer and a serial recognition elements
JP5047928B2 (en) * 2008-11-11 2012-10-10 シャープ株式会社 Electrophoresis device and components thereof
US8398839B1 (en) * 2009-06-05 2013-03-19 Sandia Corporation System for particle concentration and detection
EP2475787A2 (en) * 2009-09-07 2012-07-18 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
US8486703B2 (en) 2010-09-30 2013-07-16 Ut-Battelle, Llc Surface sampling concentration and reaction probe
WO2012141605A1 (en) * 2011-02-16 2012-10-18 Centrul International De Biodinamica Systems and method for detection and quantitation of analytes using impedance analysis
US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
WO2013039778A2 (en) 2011-09-12 2013-03-21 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
US8691067B2 (en) 2011-09-16 2014-04-08 International Business Machines Corporation Charged entities as locomotive to control motion of polymers through a nanochannel
US11198126B2 (en) * 2011-10-31 2021-12-14 Fluid-Screen, Inc. Apparatus for pathogen detection
EP3591408A1 (en) 2012-02-10 2020-01-08 The University of North Carolina at Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
GB2499428B (en) * 2012-02-16 2014-09-24 Microvisk Ltd Surface patterned micro-sensor based fluid test strip
CN104379261A (en) * 2012-04-16 2015-02-25 昆南诺股份有限公司 A nanocapillary device for biomolecule detection, a fluidic network structure and a method of manufacturing thereof
EP2682478A1 (en) * 2012-07-02 2014-01-08 Institut Curie Methods and devices for detecting macroions in a liquid medium
EP2906720A4 (en) 2012-10-10 2016-06-01 Univ Arizona Systems and devices for molecule sensing and method of manufacturing thereof
CA2902903A1 (en) * 2013-02-28 2014-09-04 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
US9970898B2 (en) 2013-03-13 2018-05-15 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US20160025702A1 (en) * 2013-03-13 2016-01-28 Arizona Board Of Regents On Behalf Of Arizona State University Systems, devices and methods for translocation control
US9677109B2 (en) 2013-03-15 2017-06-13 Accelerate Diagnostics, Inc. Rapid determination of microbial growth and antimicrobial susceptibility
CA2925112A1 (en) 2013-10-04 2015-04-09 Michigan Technological University Methods and systems for identifying a particle using dielectrophoresis
KR101664552B1 (en) * 2014-08-11 2016-10-10 엘지전자 주식회사 Apparatus for detecting particles
US10941394B2 (en) * 2015-01-15 2021-03-09 Shimadzu Corporation Device for manipulating magnetic particles and method for manipulating magnetic particles
US10253355B2 (en) 2015-03-30 2019-04-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
EP3278115A2 (en) 2015-03-30 2018-02-07 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
JPWO2017213123A1 (en) * 2016-06-07 2019-04-04 国立大学法人 東京大学 Fluid device
US10596567B2 (en) * 2017-03-27 2020-03-24 International Business Machines Corporation Microfluidic ratchets for displacing particles
DE102017121326B4 (en) 2017-09-14 2021-01-14 Leica Microsystems Cms Gmbh Collection device and method for collecting dissected or ablated specimens and microscope with such a device
US11686705B2 (en) * 2020-05-08 2023-06-27 Seagate Technology Llc Gel electrophoresis for DNA purification

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059294A (en) * 1985-12-26 1991-10-22 The University Of Puerto Rico Method for separating nucleic acids and nucleic acid probes
WO1993005390A1 (en) * 1991-08-28 1993-03-18 Guzman Norberto A Automated capillary electrophoresis apparatus
GB2266153A (en) * 1992-04-16 1993-10-20 British Tech Group Dielectrophoretic separating of a mixture of particles
US5344535A (en) * 1989-11-27 1994-09-06 British Technology Group Limited Dielectrophoretic characterization of micro-organisms and other particles
DE19500683A1 (en) * 1994-12-10 1996-06-13 Guenter Prof Dr Fuhr Trapping or movement of micro-particles in micro-structures generating electromagnetic cage field
WO1996042013A1 (en) * 1995-06-08 1996-12-27 Visible Genetics Inc. Microelectrophoresis chip for moving and separating nucleic acids and other charged molecules
WO1999063332A1 (en) * 1998-06-05 1999-12-09 Sarnoff Corporation Apparatus for separating molecules
US6007690A (en) * 1996-07-30 1999-12-28 Aclara Biosciences, Inc. Integrated microfluidic devices

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2417850A (en) 1942-04-14 1947-03-25 Willis M Winslow Method and means for translating electrical impulses into mechanical force
US4680201A (en) 1985-10-30 1987-07-14 Stellan Hjerten Coating for electrophoresis tube
US4690749A (en) 1985-12-16 1987-09-01 Universities Space Research Association Polymer-coated surfaces to control surface zeta potential
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US4874507A (en) 1986-06-06 1989-10-17 Whitlock David R Separating constituents of a mixture of particles
US4911806A (en) 1987-02-27 1990-03-27 Biotronics Method and apparatus for separating particles in liquid suspension utilizing oscillating electric and magnetic fields
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5132012A (en) 1988-06-24 1992-07-21 Hitachi, Ltd. Liquid chromatograph
US4956065A (en) 1988-11-03 1990-09-11 Kaler Karen V I S Method and apparatus for three dimensional dynamic dielectric levitation
DD279984A1 (en) 1989-02-02 1990-06-20 Univ Berlin Humboldt DIELECTRIC MICROMECHANICAL ELEMENT
US5110514A (en) 1989-05-01 1992-05-05 Soane Technologies, Inc. Controlled casting of a shrinkable material
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5275710A (en) * 1990-05-14 1994-01-04 Labintelligence, Inc. Gel electrophoresis system including optical stage, sample applicator and sample retriever
US5569591A (en) 1990-08-03 1996-10-29 University College Of Wales Aberystwyth Analytical or monitoring apparatus and method
US6149789A (en) 1990-10-31 2000-11-21 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Process for manipulating microscopic, dielectric particles and a device therefor
EP0497077B1 (en) 1991-01-28 1996-07-17 Ciba-Geigy Ag Device for preparing samples for analyses
JP3104995B2 (en) 1991-01-31 2000-10-30 ワーナー−ランバート・コンパニー Substituted heteroaryl analogs of 4,6-di-tert-butyl-5-hydroxy-1,3-pyrimidine useful as anti-inflammatory agents
DE4127405C2 (en) 1991-08-19 1996-02-29 Fraunhofer Ges Forschung Process for the separation of mixtures of microscopic dielectric particles suspended in a liquid or a gel and device for carrying out the process
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
DE59108591D1 (en) 1991-12-06 1997-04-10 Ciba Geigy Ag Electrophoretic separation device and electrophoretic separation process
US5489506A (en) 1992-10-26 1996-02-06 Biolife Systems, Inc. Dielectrophoretic cell stream sorter
AU1254295A (en) 1993-12-17 1995-07-03 Perkin-Elmer Corporation, The Uncharged polymers for separation of biomolecules by capillary electrophoresis
US5948328A (en) 1994-02-24 1999-09-07 Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Shaping of microparticles in electric-field cages
WO1995023245A1 (en) * 1994-02-25 1995-08-31 Labintelligence, Inc. Real time in-gel assay
JPH07238290A (en) * 1994-03-01 1995-09-12 Nippon Oil Co Ltd Electroviscous fluid
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6071394A (en) * 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US5459325A (en) 1994-07-19 1995-10-17 Molecular Dynamics, Inc. High-speed fluorescence scanner
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5630924A (en) * 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
US5626734A (en) * 1995-08-18 1997-05-06 University Technologies International, Inc. Filter for perfusion cultures of animal cells and the like
DE19544127C1 (en) 1995-11-27 1997-03-20 Gimsa Jan Dr Suspended particle micro-manipulation
WO1997027933A1 (en) 1996-01-31 1997-08-07 Board Of Regents, The University Of Texas System Fractionation using dielectrophoresis and field flow fractionation
AU4113297A (en) 1996-09-04 1998-03-26 Technical University Of Denmark A micro flow system for particle separation and analysis
GB9619093D0 (en) 1996-09-12 1996-10-23 Scient Generics Ltd Methods of analysis/separation
DE19653659C1 (en) 1996-12-20 1998-05-20 Guenter Prof Dr Fuhr Electrode arrangement for field cages
WO1998049344A1 (en) * 1997-04-28 1998-11-05 Lockheed Martin Energy Research Corporation Method and apparatus for analyzing nucleic acids
US5869004A (en) * 1997-06-09 1999-02-09 Caliper Technologies Corp. Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems
WO1999017883A1 (en) 1997-10-06 1999-04-15 California Institute Of Technology Electrostatic particle transportation
DE19815882A1 (en) 1998-04-08 1999-10-14 Fuhr Guenther Method and device for manipulating microparticles in fluid flows
JP2002516742A (en) 1998-05-29 2002-06-11 インダストリアル リサーチ リミテッド Method and apparatus for concentration and / or movement of particles or cells into position
US6093370A (en) * 1998-06-11 2000-07-25 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor
EP1092144A1 (en) 1998-06-29 2001-04-18 Evotec BioSystems AG Method and device for manipulating particles in microsystems
US6132685A (en) * 1998-08-10 2000-10-17 Caliper Technologies Corporation High throughput microfluidic systems and methods
US6572830B1 (en) * 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6203683B1 (en) * 1998-11-09 2001-03-20 Princeton University Electrodynamically focused thermal cycling device
DE19859461A1 (en) 1998-12-22 2000-06-29 Evotec Biosystems Ag Method and device for the convective movement of liquids in microsystems
US6352838B1 (en) * 1999-04-07 2002-03-05 The Regents Of The Universtiy Of California Microfluidic DNA sample preparation method and device
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
AU4773400A (en) 1999-05-28 2000-12-18 Leisamon (Israel), Ltd. Methods and apparatus for nonlinear mobility electrophoresis separation
US6824664B1 (en) 1999-11-04 2004-11-30 Princeton University Electrode-less dielectrophorises for polarizable particles
AU2427301A (en) 1999-12-01 2001-06-12 Regents Of The University Of California, The Electric-field-assisted fluidic assembly of inorganic and organic materials, molecules and like small things including living cells
US6537433B1 (en) * 2000-03-10 2003-03-25 Applera Corporation Methods and apparatus for the location and concentration of polar analytes using an alternating electric field
CN1319761A (en) 2000-03-15 2001-10-31 清华大学 Apparatus and method for high-flux electric rotation detection
JP2003526523A (en) 2000-03-16 2003-09-09 エスアールアイ インターナショナル Micro experimental device and method
EP1716926A3 (en) 2000-04-13 2007-08-29 Wako Pure Chemical Industries Ltd Electrode for dielectrophoretic apparatus, dielectrophoretic apparatus, method for manufacturing the same, and method for separating substances using the electrode or dielectrophoretic apparatus
EP1283900A4 (en) 2000-04-17 2006-02-08 Purdue Research Foundation Biosensor and related method
US6780584B1 (en) 2000-09-27 2004-08-24 Nanogen, Inc. Electronic systems and component devices for macroscopic and microscopic molecular biological reactions, analyses and diagnostics
CN1325909C (en) 2000-09-27 2007-07-11 清华大学 Apparatus for particle operation and guide and use method thereof
CN100495030C (en) 2000-09-30 2009-06-03 清华大学 Multi-force operator and use thereof
US6833542B2 (en) 2000-11-13 2004-12-21 Genoptix, Inc. Method for sorting particles
US6936811B2 (en) 2000-11-13 2005-08-30 Genoptix, Inc. Method for separating micro-particles
US6784420B2 (en) 2000-11-13 2004-08-31 Genoptix, Inc. Method of separating particles using an optical gradient
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
US6744038B2 (en) 2000-11-13 2004-06-01 Genoptix, Inc. Methods of separating particles using an optical gradient
US6787018B1 (en) 2000-12-08 2004-09-07 The Regents Of The University Of California Dielectrophoretic concentration of particles under electrokinetic flow
US6764583B2 (en) 2000-12-13 2004-07-20 The Regents Of The University Of California Using impedance measurements for detecting pathogens trapped in an electric field
US6685812B2 (en) 2001-01-09 2004-02-03 The Regents Of The University Of California Movement of particles using sequentially activated dielectrophoretic particle trapping
WO2002068821A2 (en) 2001-02-28 2002-09-06 Lightwave Microsystems Corporation Microfluidic control using dieletric pumping
US6706163B2 (en) 2001-03-21 2004-03-16 Michael Seul On-chip analysis of particles and fractionation of particle mixtures using light-controlled electrokinetic assembly of particles near surfaces
US6730204B2 (en) 2001-03-27 2004-05-04 The Regents Of The University Of California Three dimensional separation trap based on dielectrophoresis and use thereof
US6761811B2 (en) 2001-03-27 2004-07-13 The Regents Of The University Of California Multi-stage separations based on dielectrophoresis
US6660493B2 (en) 2001-03-28 2003-12-09 The Regents Of The University Of California Hydrodynamic enhanced dielectrophoretic particle trapping
ATE261114T1 (en) 2002-02-01 2004-03-15 Leister Process Tech MICROFLUIDIC COMPONENT AND METHOD FOR SORTING PARTICLES IN A FLUID
US6887362B2 (en) 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059294A (en) * 1985-12-26 1991-10-22 The University Of Puerto Rico Method for separating nucleic acids and nucleic acid probes
US5344535A (en) * 1989-11-27 1994-09-06 British Technology Group Limited Dielectrophoretic characterization of micro-organisms and other particles
WO1993005390A1 (en) * 1991-08-28 1993-03-18 Guzman Norberto A Automated capillary electrophoresis apparatus
GB2266153A (en) * 1992-04-16 1993-10-20 British Tech Group Dielectrophoretic separating of a mixture of particles
DE19500683A1 (en) * 1994-12-10 1996-06-13 Guenter Prof Dr Fuhr Trapping or movement of micro-particles in micro-structures generating electromagnetic cage field
WO1996042013A1 (en) * 1995-06-08 1996-12-27 Visible Genetics Inc. Microelectrophoresis chip for moving and separating nucleic acids and other charged molecules
US6007690A (en) * 1996-07-30 1999-12-28 Aclara Biosciences, Inc. Integrated microfluidic devices
WO1999063332A1 (en) * 1998-06-05 1999-12-09 Sarnoff Corporation Apparatus for separating molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FUHR G ET AL: "POSITIONING AND MANIPULATION OF CELLS AND MICROPARTICLES USING MINIATURIZED ELECTRIC FIELD TRAPS AND TRAVELLING WAVES", SENSORS AND MATERIALS, SCIENTIFIC PUBLISHING DIVISION OF MYU, TOKYO, JP, vol. 7, no. 2, 1995, pages 131 - 146, XP000617698, ISSN: 0914-4935 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

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US20030057094A1 (en) 2003-03-27
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US7704363B2 (en) 2010-04-27
US6969454B2 (en) 2005-11-29
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AU2001245558A1 (en) 2001-09-24
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