WO2001068889A3 - Method for gene transfection and vaccination through skin using electropermeabilization - Google Patents

Method for gene transfection and vaccination through skin using electropermeabilization Download PDF

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Publication number
WO2001068889A3
WO2001068889A3 PCT/US2001/008502 US0108502W WO0168889A3 WO 2001068889 A3 WO2001068889 A3 WO 2001068889A3 US 0108502 W US0108502 W US 0108502W WO 0168889 A3 WO0168889 A3 WO 0168889A3
Authority
WO
WIPO (PCT)
Prior art keywords
skin
gene
polynucleotide
approach
individual
Prior art date
Application number
PCT/US2001/008502
Other languages
French (fr)
Other versions
WO2001068889A2 (en
Inventor
Robert W Malone
Jill G Malone
Joseph J Drabick
Original Assignee
Robert W Malone
Jill G Malone
Joseph J Drabick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert W Malone, Jill G Malone, Joseph J Drabick filed Critical Robert W Malone
Priority to AU2001250859A priority Critical patent/AU2001250859A1/en
Publication of WO2001068889A2 publication Critical patent/WO2001068889A2/en
Publication of WO2001068889A3 publication Critical patent/WO2001068889A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/29—Hepatitis virus
    • A61K39/292—Serum hepatitis virus, hepatitis B virus, e.g. Australia antigen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0075—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the delivery route, e.g. oral, subcutaneous
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/20—Applying electric currents by contact electrodes continuous direct currents
    • A61N1/30—Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0408—Use-related aspects
    • A61N1/0412—Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
    • A61N1/0416—Anode and cathode
    • A61N1/0424—Shape of the electrode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Abstract

Cells in the skin or mucosal tissue of an individual can be transfected with a polynucleotide or gene by injecting the polynucleotide into the skin or mucosal tissue and applying a pulsating electrical field in the vicinity of the injection. This approach is suitable for vaccinating an individual to the expressed polypeptide, thereby generating antibodies or to engendering cell-mediated immunity. For example, antibodies generated against the expression product of a gene cloned from genomic or cDNA libraries can be used to characterize the product and, hence, illuminate the function of the gene. The approach also can be employed, in an animal or human, for treating a disease condition or for modifying a skin condition that affects the appearance of the skin. Kits are included for carrying out the methods of the invention.
PCT/US2001/008502 2000-03-16 2001-03-16 Method for gene transfection and vaccination through skin using electropermeabilization WO2001068889A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001250859A AU2001250859A1 (en) 2000-03-16 2001-03-16 Method for gene transfection and vaccination through skin using electropermeabilization

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18984700A 2000-03-16 2000-03-16
US60/189,847 2000-03-16

Publications (2)

Publication Number Publication Date
WO2001068889A2 WO2001068889A2 (en) 2001-09-20
WO2001068889A3 true WO2001068889A3 (en) 2002-04-25

Family

ID=37488067

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/008502 WO2001068889A2 (en) 2000-03-16 2001-03-16 Method for gene transfection and vaccination through skin using electropermeabilization

Country Status (2)

Country Link
AU (1) AU2001250859A1 (en)
WO (1) WO2001068889A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006119983A2 (en) 2005-05-12 2006-11-16 Novartis Ag Genes and proteins of brachyspira hyodysenteriae and use of same for diagnosis and therapy
US20080033398A1 (en) * 2005-12-29 2008-02-07 Transcutaneous Technologies Inc. Device and method for enhancing immune response by electrical stimulation
EP3018139A3 (en) 2007-08-03 2016-08-10 Boehringer Ingelheim Vetmedica GmbH Genes and proteins of brachyspira hyodysenteriae and uses thereof
GB0720250D0 (en) 2007-10-17 2007-11-28 Univ Edinburgh Immunogenic compositions containing escherichia coli h7 flagella and methods of use thereof
EP2363407A1 (en) 2008-02-28 2011-09-07 Murdoch University Novel sequences of Brachyspira, immunogenic compositions, methods for preparation and use thereof
WO2009117773A1 (en) 2008-03-27 2009-10-01 Murdoch University Novel sequences of brachyspira, immunogenic compositions, methods for preparation and use thereof
ITCO20080016A1 (en) * 2008-04-22 2009-10-23 Giuseppe Caccia HANDPIECE FOR THE ELECTROPORATION OF PHARMACOLOGICAL AND / OR NATURAL AND / OR AESTHETIC PRODUCTS ON LAYERS CUTANEOUS WITH THICKNESS CONTENT

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5019034A (en) * 1988-01-21 1991-05-28 Massachusetts Institute Of Technology Control of transport of molecules across tissue using electroporation
WO1991018103A1 (en) * 1990-05-16 1991-11-28 Scientific Equipment Design & Development S.C. Method and device for making living cells permeable
WO1996039531A1 (en) * 1995-06-06 1996-12-12 Massachusetts Institute Of Technology Delivery of nucleotides into organisms by electroporation
WO1997007826A1 (en) * 1995-08-29 1997-03-06 Cbr Laboratories, Inc. In vivo electroporation of cells
WO1999064615A1 (en) * 1998-06-08 1999-12-16 Valentis, Inc. Formulations for electroporation

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5019034A (en) * 1988-01-21 1991-05-28 Massachusetts Institute Of Technology Control of transport of molecules across tissue using electroporation
US5019034B1 (en) * 1988-01-21 1995-08-15 Massachusetts Inst Technology Control of transport of molecules across tissue using electroporation
WO1991018103A1 (en) * 1990-05-16 1991-11-28 Scientific Equipment Design & Development S.C. Method and device for making living cells permeable
WO1996039531A1 (en) * 1995-06-06 1996-12-12 Massachusetts Institute Of Technology Delivery of nucleotides into organisms by electroporation
WO1997007826A1 (en) * 1995-08-29 1997-03-06 Cbr Laboratories, Inc. In vivo electroporation of cells
WO1999064615A1 (en) * 1998-06-08 1999-12-16 Valentis, Inc. Formulations for electroporation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BANGA A K ET AL: "Assessing the potential of skin electroporation for the delivery of protein- and gene-based drugs", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 16, no. 10, 1 October 1998 (1998-10-01), pages 408 - 412, XP004145647, ISSN: 0167-7799 *
BARON S ET AL: "Electroporation of antibodies, DNA, and other macromolecules into cells: a highly efficient method", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 242, no. 1-2, 28 August 2000 (2000-08-28), pages 115 - 126, XP004210715, ISSN: 0022-1759 *
JOSEPH J. DRABICK ET AL: "Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid accination Are Significantly Enhanced by in Vivo Electropermeabilization", MOLECULAR THERAPY, vol. 3, no. 2, February 2001 (2001-02-01), pages 249 - 255, XP002190195 *
SELBY M ET AL: "Enhancement of DNA vaccine potency by electroporation in vivo", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 83, no. 1-2, 29 September 2000 (2000-09-29), pages 147 - 152, XP004212162, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
WO2001068889A2 (en) 2001-09-20
AU2001250859A1 (en) 2001-09-24

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