WO2001057269A3 - Nucleic acid detection methods using universal priming - Google Patents

Nucleic acid detection methods using universal priming Download PDF

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Publication number
WO2001057269A3
WO2001057269A3 PCT/US2001/004056 US0104056W WO0157269A3 WO 2001057269 A3 WO2001057269 A3 WO 2001057269A3 US 0104056 W US0104056 W US 0104056W WO 0157269 A3 WO0157269 A3 WO 0157269A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
detection methods
acid detection
universal priming
priming
Prior art date
Application number
PCT/US2001/004056
Other languages
French (fr)
Other versions
WO2001057269A2 (en
Inventor
Jian-Bing Fan
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Illumina Inc
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26876658&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2001057269(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to JP2001555892A priority Critical patent/JP2003521252A/en
Priority to AU2001238068A priority patent/AU2001238068A1/en
Priority to DE60136166T priority patent/DE60136166D1/en
Priority to CA2399733A priority patent/CA2399733C/en
Priority to DK01910466T priority patent/DK1259643T3/en
Application filed by Illumina Inc filed Critical Illumina Inc
Priority to EP01910466A priority patent/EP1259643B1/en
Publication of WO2001057269A2 publication Critical patent/WO2001057269A2/en
Publication of WO2001057269A3 publication Critical patent/WO2001057269A3/en
Priority to HK02109139.1A priority patent/HK1047603B/en
Priority to US10/620,852 priority patent/US20040121364A1/en
Priority to US11/036,886 priority patent/US20050214825A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Abstract

The present invention is directed to providing sensitive and accurate assays for genotyping with a minimum or absence of target-specific amplification.
PCT/US2001/004056 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming WO2001057269A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
EP01910466A EP1259643B1 (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming
AU2001238068A AU2001238068A1 (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming
DE60136166T DE60136166D1 (en) 2000-02-07 2001-02-07 NUCLEIC ACID PROOF METHOD WITH UNIVERSAL PRIMING
CA2399733A CA2399733C (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming
DK01910466T DK1259643T3 (en) 2000-02-07 2001-02-07 Method for Detecting Nucleic Acid Using Universal Priming
JP2001555892A JP2003521252A (en) 2000-02-07 2001-02-07 Nucleic acid detection method using universal priming
HK02109139.1A HK1047603B (en) 2000-02-07 2002-12-17 Nucleic acid detection methods using universal priming
US10/620,852 US20040121364A1 (en) 2000-02-07 2003-07-15 Multiplex nucleic acid reactions
US11/036,886 US20050214825A1 (en) 2000-02-07 2005-01-14 Multiplex sample analysis on universal arrays

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18081000P 2000-02-07 2000-02-07
US60/180,810 2000-02-07
US23473200P 2000-09-22 2000-09-22
US60/234,732 2000-09-22

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/931,285 Continuation-In-Part US6913884B2 (en) 2000-02-07 2001-08-16 Compositions and methods for repetitive use of genomic DNA

Publications (2)

Publication Number Publication Date
WO2001057269A2 WO2001057269A2 (en) 2001-08-09
WO2001057269A3 true WO2001057269A3 (en) 2002-07-18

Family

ID=26876658

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004056 WO2001057269A2 (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming

Country Status (10)

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US (5) US20020006617A1 (en)
EP (2) EP1259643B1 (en)
JP (1) JP2003521252A (en)
AT (2) ATE492652T1 (en)
AU (1) AU2001238068A1 (en)
CA (1) CA2399733C (en)
DE (2) DE60143723D1 (en)
DK (1) DK1259643T3 (en)
HK (1) HK1047603B (en)
WO (1) WO2001057269A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions
US9399795B2 (en) 1998-06-24 2016-07-26 Illumina, Inc. Multiplex decoding of array sensors with microspheres

Families Citing this family (335)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
US6720149B1 (en) * 1995-06-07 2004-04-13 Affymetrix, Inc. Methods for concurrently processing multiple biological chip assays
US6251691B1 (en) 1996-04-25 2001-06-26 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US7510841B2 (en) 1998-12-28 2009-03-31 Illumina, Inc. Methods of making and using composite arrays for the detection of a plurality of target analytes
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) * 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
EP1257664A4 (en) * 2000-01-28 2006-04-05 Althea Technologies Inc Methods for analysis of gene expression
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US6770441B2 (en) * 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
CA2410950A1 (en) * 2000-05-30 2001-12-06 Hans-Michael Wenz Methods for detecting target nucleic acids using coupled ligation and amplification
EP1311839B1 (en) 2000-06-21 2006-03-01 Bioarray Solutions Ltd Multianalyte molecular analysis using application-specific random particle arrays
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
AU2001272629A1 (en) * 2000-07-06 2002-01-14 Bio Merieux Method for controlling the microbiological quality of an aqueous medium and kit therefor
JP2004526448A (en) * 2001-03-30 2004-09-02 アプレラ コーポレイション Nucleic acid analysis using non-template nucleotide addition
US7572642B2 (en) 2001-04-18 2009-08-11 Ambrigen, Llc Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns
WO2002090599A1 (en) 2001-05-09 2002-11-14 Genetic Id, Inc. Universal microarray system
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20040002073A1 (en) 2001-10-15 2004-01-01 Li Alice Xiang Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
EP1456416B1 (en) * 2001-11-19 2009-03-11 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
WO2003045310A2 (en) * 2001-11-21 2003-06-05 Applera Corporation Digital assay
EP1860200A1 (en) * 2001-12-03 2007-11-28 Illumina, Inc. Multiplexed methylation detection methods
ATE431407T1 (en) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo METHOD FOR PRODUCING DNA
US7771922B2 (en) * 2002-05-03 2010-08-10 Kimberly-Clark Worldwide, Inc. Biomolecule diagnostic device
AU2003269908A1 (en) * 2002-07-15 2004-02-02 Illumina, Inc. Multiplex nucleic acid reactions
CN1182256C (en) * 2002-08-09 2004-12-29 周国华 Gene expression amount comparing analysis method
ATE520027T1 (en) * 2002-09-03 2011-08-15 Bayer Technology Services Gmbh ANALYTICAL PLATFORM AND DETECTION METHOD WITH ANALYTES TO BE DETECTED IN A SAMPLE AS IMMOBILIZED SPECIFIC BONDING PARTNERS, IF APPLICABLE AFTER FRACTIONATION
EP1552001A4 (en) * 2002-09-19 2006-01-04 Applera Corp Methods and compositions for detecting targets
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
CA2930400A1 (en) * 2003-02-26 2004-09-10 Callida Genomics, Inc. Apparatus with pixel alignment system for analyzing nucleic acid
DE602004021569D1 (en) * 2003-03-07 2009-07-30 Univ New Jersey Med OPTICALLY DECODIBLE MICRO BEARINGS, ARRAYS AND METHOD
US8003374B2 (en) 2003-03-25 2011-08-23 The Regents Of The University Of California Reagentless, reusable, bioelectronic detectors
US20040191801A1 (en) * 2003-03-25 2004-09-30 Heeger Alan J. Reagentless, reusable bioelectronic detectors and their use as authentication devices
US20050064452A1 (en) * 2003-04-25 2005-03-24 Schmid Matthew J. System and method for the detection of analytes
JP2007525963A (en) * 2003-06-20 2007-09-13 イルミナ インコーポレイテッド Methods and compositions for whole genome amplification and genotyping
WO2005012899A2 (en) * 2003-07-28 2005-02-10 Applera Corporation Method for error detection and increased confidence of sample decoding
ATE419394T1 (en) * 2003-09-04 2009-01-15 Human Genetic Signatures Pty NUCLEIC ACID DETECTION TEST
WO2005026686A2 (en) * 2003-09-09 2005-03-24 Compass Genetics, Llc Multiplexed analytical platform
US7824856B2 (en) * 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
US20050266417A1 (en) * 2003-09-12 2005-12-01 Francis Barany Methods for identifying target nucleic acid molecules
US7927796B2 (en) 2003-09-18 2011-04-19 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
US20050100939A1 (en) * 2003-09-18 2005-05-12 Eugeni Namsaraev System and methods for enhancing signal-to-noise ratios of microarray-based measurements
WO2005031305A2 (en) 2003-09-22 2005-04-07 Bioarray Solutions, Ltd. Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US7563569B2 (en) 2003-10-28 2009-07-21 Michael Seul Optimization of gene expression analysis using immobilized capture probes
JP2007509629A (en) 2003-10-29 2007-04-19 バイオアレイ ソリューションズ リミテッド Complex nucleic acid analysis by cleavage of double-stranded DNA
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
WO2005080604A2 (en) 2004-02-12 2005-09-01 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
WO2005111242A2 (en) * 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US7785843B2 (en) * 2004-06-23 2010-08-31 Sequenom, Inc. Target-specific compomers and methods of use
US20060029954A1 (en) * 2004-06-30 2006-02-09 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
US20060019304A1 (en) * 2004-07-26 2006-01-26 Paul Hardenbol Simultaneous analysis of multiple genomes
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US7585664B2 (en) * 2004-10-14 2009-09-08 The Hong Kong University Of Science And Technology Integrated circuit optical detector for biological detection
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US20060211030A1 (en) * 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
US20060228735A1 (en) * 2005-03-31 2006-10-12 Perkinelmer Las, Inc. Multiplex assay systems
US20060246576A1 (en) 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
AU2006243757B2 (en) * 2005-05-03 2012-02-09 Altheadx, Inc. Compositions and methods for the analysis of degraded nucleic acids
US8486629B2 (en) * 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
US7368242B2 (en) * 2005-06-14 2008-05-06 Affymetrix, Inc. Method and kits for multiplex hybridization assays
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
EP3492602A1 (en) 2005-06-15 2019-06-05 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US9297036B2 (en) * 2005-07-01 2016-03-29 Agilent Technologies, Inc Nucleic acid probes for analysis of small RNAs and other polynucleotides
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US20070054301A1 (en) * 2005-09-06 2007-03-08 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US20070077582A1 (en) * 2005-09-16 2007-04-05 Primera Biosystems, Inc. Method for quantitative detection of short RNA molecules
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070099193A1 (en) * 2005-11-01 2007-05-03 Hui Wang Probe/target stabilization with add-in oligo
CN101268201A (en) * 2005-11-08 2008-09-17 奥林巴斯株式会社 Method of amplifying multiple nucleic acid sequences for differentiation
WO2007120299A2 (en) * 2005-11-29 2007-10-25 The Regents Of The University Of California Signal-on architecture for electronic, oligonucleotide-based detectors
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
WO2007087312A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070172841A1 (en) * 2006-01-25 2007-07-26 Hui Wang Probe/target stabilization with add-in oligo
US7754475B2 (en) * 2006-01-25 2010-07-13 Agilent Technologies, Inc. Nucleic acid probes and microarrays for analysis of polynucleotides
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
EP2495337A1 (en) * 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
US8673567B2 (en) * 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
US7914988B1 (en) * 2006-03-31 2011-03-29 Illumina, Inc. Gene expression profiles to predict relapse of prostate cancer
WO2007120843A2 (en) * 2006-04-12 2007-10-25 Siemens Healthcare Diagnostics Inc. Single nucleotide polymorphism detection from unamplified genomic dna
US20080124726A1 (en) * 2006-05-26 2008-05-29 Althea Technologies, Inc. Biochemical analysis of partitioned cells
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2366801B1 (en) 2006-06-14 2016-10-19 Verinata Health, Inc Methods for the diagnosis of fetal abnormalities
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
EP3424598B1 (en) 2006-06-14 2022-06-08 Verinata Health, Inc. Rare cell analysis using sample splitting and dna tags
CA2664649A1 (en) * 2006-09-28 2008-05-08 Ensemble Discovery Corporation Compositions and methods for biodetection by nucleic acid-templated chemistry
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
US20090111705A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
US7902345B2 (en) * 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US8133701B2 (en) * 2006-12-05 2012-03-13 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CA2673017C (en) 2006-12-21 2015-08-04 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US20080241831A1 (en) * 2007-03-28 2008-10-02 Jian-Bing Fan Methods for detecting small RNA species
US8003314B2 (en) 2007-04-16 2011-08-23 Diagnostic Hybrids, Inc. Methods for direct fluorescent antibody virus detection in liquids
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US7769548B2 (en) * 2007-05-10 2010-08-03 Illumina, Inc. Microarray analytical data stitching system and method
EP2162548A1 (en) * 2007-05-23 2010-03-17 Oregon Health and Science University Microarray systems and methods for identifying dna-binding proteins
WO2009011888A2 (en) * 2007-07-16 2009-01-22 Massachusetts Institute Of Technology Ruler arrays
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US8951731B2 (en) * 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8617811B2 (en) * 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
WO2009073629A2 (en) * 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
WO2009091934A1 (en) 2008-01-17 2009-07-23 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP2009268665A (en) * 2008-05-07 2009-11-19 Canon Inc Inhalation device
CN102203282B (en) 2008-06-25 2014-04-30 生命技术公司 Methods and apparatus for measuring analytes using large scale FET arrays
CN102428191A (en) * 2009-03-18 2012-04-25 塞昆纳姆股份有限公司 Use Of Thermostable Endonucleases For Generating Reporter Molecules
US20110171619A1 (en) * 2009-05-28 2011-07-14 Daniel Leo Sweeney Representation of molecules as sets of masses of complementary subgroups and contiguous complementary subgroups
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
EP2449104B1 (en) * 2009-06-29 2014-06-04 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
EP2449132B1 (en) 2009-07-01 2015-05-13 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
EP2280079A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Ligation-based method of normalized quantification of nucleic acids
EP2280080A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
US9487839B2 (en) * 2009-09-29 2016-11-08 Case Western Reserve University Method for detecting single nucleotide polymorphisms
US8975019B2 (en) * 2009-10-19 2015-03-10 University Of Massachusetts Deducing exon connectivity by RNA-templated DNA ligation/sequencing
CA2779750C (en) 2009-11-06 2019-03-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
WO2011071923A2 (en) * 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US9499814B2 (en) 2010-01-22 2016-11-22 The Regents Of The University Of California Methods for identifying drug effects on a cell by determining changes in the cell's spliced message profile
US20130059736A1 (en) * 2010-02-05 2013-03-07 Institute For Systems Biology Methods and compositions for profiling rna molecules
US9371598B2 (en) 2010-04-05 2016-06-21 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
EP2572003A4 (en) 2010-05-18 2016-01-13 Natera Inc Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
CA2802111A1 (en) * 2010-07-23 2012-01-26 Esoterix Genetic Laboratories, Llc Identification of differentially represented fetal or maternal genomic regions and uses thereof
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20130261003A1 (en) * 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10167508B2 (en) * 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
EP2623613B8 (en) 2010-09-21 2016-09-07 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
WO2012054933A2 (en) * 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US20120190020A1 (en) * 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
EP2683833B1 (en) 2011-03-10 2018-09-26 Gen-Probe Incorporated Methods for the selection and optimization of oligonucleotide tag sequences
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012145574A2 (en) 2011-04-20 2012-10-26 Life Technologies Corporation Methods, compositions and systems for sample deposition
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
JP6404714B2 (en) * 2011-06-24 2018-10-17 ナノストリング テクノロジーズ,インコーポレイティド Multivariate diagnostic assay and method for using the same
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
EP2758538B1 (en) 2011-09-22 2018-10-31 Lineage Biosciences, Inc. Methods for analyzing heterogeneous samples
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
US8778849B2 (en) 2011-10-28 2014-07-15 Illumina, Inc. Microarray fabrication system and method
US9200274B2 (en) 2011-12-09 2015-12-01 Illumina, Inc. Expanded radix for polymeric tags
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
WO2013128281A1 (en) 2012-02-28 2013-09-06 Population Genetics Technologies Ltd Method for attaching a counter sequence to a nucleic acid sample
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CN104812947B (en) * 2012-07-17 2018-04-27 考希尔股份有限公司 The system and method for detecting hereditary variation
EP2875156A4 (en) 2012-07-19 2016-02-24 Ariosa Diagnostics Inc Multiplexed sequential ligation-based detection of genetic variants
RU2650790C2 (en) * 2012-07-24 2018-04-17 Натера, Инк. Highly multiplex pcr methods and compositions
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
MX367963B (en) 2012-09-04 2019-09-11 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation.
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9222122B2 (en) 2012-11-20 2015-12-29 Src, Inc. System and method for rapid detection and identification of nucleic acid labeled tags
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
AU2013382089B2 (en) 2013-03-13 2018-05-10 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
EP2970959B1 (en) 2013-03-15 2018-05-30 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
PL3017065T3 (en) 2013-07-01 2019-03-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
MX371428B (en) 2013-08-19 2020-01-30 Singular Bio Inc Assays for single molecule detection and use thereof.
KR102536833B1 (en) 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
JP2016539343A (en) 2013-08-30 2016-12-15 イルミナ インコーポレイテッド Manipulating droplets on hydrophilic or mottled hydrophilic surfaces
CN105745528A (en) 2013-10-07 2016-07-06 赛卢拉研究公司 Methods and systems for digitally counting features on arrays
WO2015070103A1 (en) 2013-11-11 2015-05-14 Life Technologies Corporation Rotor assembly and method for using same
EP3556869B1 (en) 2013-11-26 2023-08-02 Illumina Inc. Compositions and methods for polynucleotide sequencing
EP3778890B1 (en) 2013-12-23 2023-12-06 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
EP3087204B1 (en) 2013-12-28 2018-02-14 Guardant Health, Inc. Methods and systems for detecting genetic variants
JP6666852B2 (en) 2014-01-16 2020-03-18 イルミナ インコーポレイテッド Gene expression panel for prognosis of prostate cancer recurrence
EP3094742A1 (en) 2014-01-16 2016-11-23 Illumina, Inc. Amplicon preparation and sequencing on solid supports
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
EP3561075A1 (en) 2014-04-21 2019-10-30 Natera, Inc. Detecting mutations in tumour biopsies and cell-free samples
SG11201700348VA (en) 2014-07-15 2017-02-27 Illumina Inc Biochemically activated electronic device
EP3183577B1 (en) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible surface functionalization
PT3212684T (en) 2014-10-31 2020-02-03 Illumina Cambridge Ltd Novel polymers and dna copolymer coatings
JP6616574B2 (en) * 2015-01-30 2019-12-04 倉敷紡績株式会社 Visually identifiable genetic test
CN113637729B (en) 2015-02-18 2024-02-23 因威塔公司 Assay for single molecule detection and uses thereof
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
CN107208158B (en) 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
FI3901281T3 (en) 2015-04-10 2023-01-31 Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016168386A1 (en) 2015-04-14 2016-10-20 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3294906A1 (en) 2015-05-11 2018-03-21 Natera, Inc. Methods and compositions for determining ploidy
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
JP6698708B2 (en) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション Methods, systems, compositions, kits, devices, and computer-readable media for molecular tagging
WO2017007753A1 (en) 2015-07-07 2017-01-12 Illumina, Inc. Selective surface patterning via nanoimrinting
EP3325648B1 (en) 2015-07-17 2023-03-29 Illumina, Inc. Polymer sheets for sequencing applications
CN107921432A (en) 2015-09-02 2018-04-17 伊卢米纳剑桥有限公司 Improve the system and method for the droplet manipulation in flow control system
CN108026524A (en) 2015-09-11 2018-05-11 赛卢拉研究公司 Method and composition for nucleic acid library standardization
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
EP3390657B1 (en) 2015-12-17 2020-09-16 Illumina, Inc. Distinguishing methylation levels in complex biological samples
CA3008651A1 (en) 2015-12-17 2017-06-22 Guardant Health, Inc. Methods to determine tumor gene copy number by analysis of cell-free dna
AU2017261189B2 (en) 2016-05-02 2023-02-09 Becton, Dickinson And Company Accurate molecular barcoding
AU2017267653B2 (en) 2016-05-18 2021-05-13 Illumina, Inc. Self assembled patterning using patterned Hydrophobic surfaces
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US11460468B2 (en) 2016-09-26 2022-10-04 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
EP3538672A1 (en) 2016-11-08 2019-09-18 Cellular Research, Inc. Methods for cell label classification
KR20190077062A (en) 2016-11-08 2019-07-02 셀룰러 리서치, 인크. How to classify expression profiles
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN110573253B (en) 2017-01-13 2021-11-02 赛卢拉研究公司 Hydrophilic coating for fluid channels
AU2017394644B2 (en) 2017-01-20 2020-02-06 Pacific Biosciences Of California, Inc. Allele-specific capture of nucleic acids
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10852274B2 (en) 2017-03-09 2020-12-01 Auburn University Differential circuit for background correction in electrochemical measurements
CN110612351B (en) * 2017-03-20 2023-08-11 Illumina公司 Methods and compositions for preparing nucleic acid libraries
CN106929593B (en) * 2017-04-27 2021-04-30 华侨大学 In-situ nucleic acid detection method
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
WO2019010341A1 (en) 2017-07-07 2019-01-10 Innamed, Inc. Aptamers for measuring lipoprotein levels
SG11201911869XA (en) 2017-08-01 2020-01-30 Illumina Inc Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
NZ759924A (en) 2017-08-01 2023-07-28 Illumina Inc Hydrogel beads for nucleotide sequencing
CN109486922B (en) * 2017-09-08 2022-07-29 深圳华大因源医药科技有限公司 Method for detecting microbial target sequence based on single primer probe capture
US11946095B2 (en) 2017-12-19 2024-04-02 Becton, Dickinson And Company Particles associated with oligonucleotides
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
CA3186025A1 (en) 2018-02-13 2019-08-22 Illumina, Inc. Dna sequencing using hydrogel beads
US10871485B2 (en) 2018-04-13 2020-12-22 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
SG11201911961RA (en) 2018-04-20 2020-01-30 Illumina Inc Methods of encapsulating single cells, the encapsulated cells and uses thereof
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
EP3807420A1 (en) 2018-06-12 2021-04-21 Keygene N.V. Nucleic acid amplification method
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112770776A (en) 2018-07-30 2021-05-07 瑞德库尔有限责任公司 Method and system for sample processing or analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
JP2022511398A (en) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー Determining the 5'transcription sequence
BR112021005976A2 (en) 2018-10-26 2021-06-29 Illumina, Inc. modulation of polymer microspheres for DNA processing
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
BR112021006183A2 (en) 2018-11-30 2021-06-29 Illumina, Inc. analysis of multiple analytes using a single assay
CA3103736A1 (en) 2018-12-05 2020-06-11 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
EP3894590A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
AU2019411272A1 (en) 2018-12-18 2021-01-07 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
DK3899037T3 (en) 2018-12-19 2023-11-06 Illumina Inc METHODS OF IMPROVING POLYNUCLEOTIDE CLUSTER CLONALITY PRIORITY
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
CN113474466A (en) 2019-02-21 2021-10-01 主基因有限公司 Polyploid genotyping
US20220154173A1 (en) 2019-07-12 2022-05-19 Iiiumina Cambridge Limited Compositions and Methods for Preparing Nucleic Acid Sequencing Libraries Using CRISPR/CAS9 Immobilized on a Solid Support
CN114051534A (en) 2019-07-22 2022-02-15 贝克顿迪金森公司 Single cell chromatin immunoprecipitation sequencing assay
CN110592201A (en) * 2019-10-30 2019-12-20 上海千履基因科技有限公司 PCR amplification method of one-tube type high-specificity nucleic acid product, rapid nucleic acid detection method and nucleic acid detection test strip
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
CN114885610A (en) 2019-12-23 2022-08-09 10X基因组学有限公司 Methods for spatial analysis using RNA templated ligation
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4242325A3 (en) 2020-04-22 2023-10-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
CN115605614A (en) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) Primers for immune repertoire profiling
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4162074A1 (en) 2020-06-08 2023-04-12 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
EP4172362A1 (en) 2020-06-25 2023-05-03 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
AU2021320307A1 (en) 2020-08-06 2023-02-16 Illumina Cambridge Limited Preparation of RNA and DNA sequencing libraries using bead-linked transposomes
IL299783A (en) 2020-08-18 2023-03-01 Illumina Inc Sequence-specific targeted transposition and selection and sorting of nucleic acids
WO2022053610A1 (en) 2020-09-11 2022-03-17 Illumina Cambridge Limited Methods of enriching a target sequence from a sequencing library using hairpin adaptors
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4288562A1 (en) 2021-02-04 2023-12-13 Illumina, Inc. Long indexed-linked read generation on transposome bound beads
AU2022245985A1 (en) 2021-03-22 2023-09-21 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
BR112023019894A2 (en) 2021-03-29 2023-11-14 Illumina Inc COMPOSITIONS AND METHODS FOR ASSESSING DNA DAMAGE IN A LIBRARY AND NORMALIZING AMPLICON SIZE DISTORTION
IL307195A (en) 2021-03-29 2023-11-01 Illumina Inc Improved methods of library preparation
AU2022249289A1 (en) 2021-03-31 2023-08-17 Illumina Cambridge Limited Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction
CN117337334A (en) * 2021-05-21 2024-01-02 上海绾塍生物科技有限公司 Compositions and methods for analyzing target molecules from a sample
WO2022265994A1 (en) 2021-06-15 2022-12-22 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US20230116852A1 (en) 2021-07-23 2023-04-13 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
CN113463203A (en) * 2021-07-27 2021-10-01 华侨大学 Construction method of in-situ sequencing library for realizing multiple RNA in-situ detection
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023220056A1 (en) * 2022-05-10 2023-11-16 Northwestern University Organic electrochemical transistor as an on-site signal amplifier for electrochemical aptamer-based sensors

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
WO1997045559A1 (en) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
WO1998004746A1 (en) * 1996-07-31 1998-02-05 Mount Sinai School Of Medicine Of The City University Of New York Nucleic acid amplification method: ramification-extension amplification method (ram)

Family Cites Families (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4200110A (en) 1977-11-28 1980-04-29 United States Of America Fiber optic pH probe
FI63596C (en) * 1981-10-16 1983-07-11 Orion Yhtymae Oy MICROBIA DIAGNOSIS FOERFARANDE SOM GRUNDAR SIG PAO SKIKTSHYBRIDISERING AV NUCLEINSYROR OCH VID FOERFARANDET ANVAENDA KOMBINATIONER AV REAGENSER
US4499052A (en) 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4687732A (en) * 1983-06-10 1987-08-18 Yale University Visualization polymers and their application to diagnostic medicine
US4582789A (en) * 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US4682895A (en) 1985-08-06 1987-07-28 Texas A&M University Fiber optic probe for quantification of colorimetric reactions
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
US4824789B1 (en) 1986-10-10 1996-08-13 Minnesota Mining & Mfg Gas sensor
US4798738A (en) 1986-10-10 1989-01-17 Cardiovascular Devices, Inc. Micro sensor
US5114864A (en) 1986-06-25 1992-05-19 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using fluid erodible controlled release polymers for delivery of reagent formulations
US5143853A (en) 1986-06-25 1992-09-01 Trustees Of Tufts College Absorbance modulated fluorescence detection methods and sensors
US5254477A (en) 1986-06-25 1993-10-19 Trustees Of Tufts College Flourescence intramolecular energy transfer conjugate compositions and detection methods
US4822746A (en) 1986-06-25 1989-04-18 Trustees Of Tufts College Radiative and non-radiative energy transfer and absorbance modulated fluorescence detection methods and sensors
US5252494A (en) 1986-06-25 1993-10-12 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix
DE3681176D1 (en) 1986-12-01 1991-10-02 Molecular Biosystems Inc METHOD FOR INCREASING THE SENSITIVITY OF NUCLEIC ACID HYBRIDIZATION TESTS.
SE458968B (en) 1987-06-16 1989-05-22 Wallac Oy BIOSPECIFIC ANALYTICAL PROCEDURE FOR MULTIPLE ANALYTICS WHICH DO NOT INCLUDE PARTICULAR COATING AND LABELING WITH FLUORESCING LABEL SUBSTANCES
US5132242A (en) 1987-07-15 1992-07-21 Cheung Sau W Fluorescent microspheres and methods of using them
US5194300A (en) 1987-07-15 1993-03-16 Cheung Sau W Methods of making fluorescent microspheres
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US4785814A (en) 1987-08-11 1988-11-22 Cordis Corporation Optical probe for measuring pH and oxygen in blood and employing a composite membrane
US5403711A (en) * 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5104791A (en) * 1988-02-09 1992-04-14 E. I. Du Pont De Nemours And Company Particle counting nucleic acid hybridization assays
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
NO164622C (en) 1988-05-11 1990-10-24 Tore Lindmo BINAER IMMUNOMETRIC PARTICLE-BASED METHOD FOR MEASURING SPECIFIC SERUM ANTIGENS USING LIQUID FLOW MICROPHOTOMETRY AND A PREPARED TARGET SET UP THEREOF.
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5575849A (en) 1988-11-25 1996-11-19 Canon Kabushiki Kaisha Apparatus for producing a substrate having a surface with a plurality of spherical dimples for photoconductive members
JPH02299598A (en) 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res Determination by means of hybridization, together with oligonucleotide probe of all or part of extremely short sequence in sample of nucleic acid connecting with separate particle of microscopic size
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5871928A (en) * 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
EP0439182B1 (en) * 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
DE69125441T2 (en) 1990-09-28 1997-11-06 Toshiba Kawasaki Kk Gene detection method
EP0557456A4 (en) 1990-11-14 1995-11-15 Siska Diagnostics Inc Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5105305A (en) 1991-01-10 1992-04-14 At&T Bell Laboratories Near-field scanning optical microscope using a fluorescent probe
US5244636A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample
US5250264A (en) 1991-01-25 1993-10-05 Trustees Of Tufts College Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5244813A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
EP0519338B1 (en) * 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
ATE171543T1 (en) 1991-07-16 1998-10-15 Transmed Biotech Inc METHODS AND COMPOSITIONS FOR THE SIMULTANEOUS ANALYSIS OF A MULTIPLE ANALYTES
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
ES2112916T3 (en) 1991-10-15 1998-04-16 Multilyte Ltd UNION TEST USING A MARKED REAGENT.
DE69232753T2 (en) * 1991-11-01 2003-05-15 Diatech Pty Ltd SOLIDS PHASE EXPANSION PROCESS
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5888723A (en) 1992-02-18 1999-03-30 Johnson & Johnson Clinical Diagnostics, Inc. Method for nucleic acid amplification and detection using adhered probes
US5869252A (en) * 1992-03-31 1999-02-09 Abbott Laboratories Method of multiplex ligase chain reaction
EP0565999A2 (en) 1992-04-16 1993-10-20 Siemens Aktiengesellschaft Optical coupling device for two groups of waveguides
WO1993022461A1 (en) 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
CA2115342C (en) 1992-06-17 2003-08-26 Robert B. Wallace A method of detecting and discriminating between nucleic acid sequences
AU4779493A (en) 1992-07-21 1994-02-14 Bunsen Rush Laboratories Inc. Oligomer library formats and methods relating thereto
DE69320484T2 (en) 1992-09-14 1998-12-24 Stanford Res Inst Int "UP-CONVERTING" REPORTER MOLECULE FOR BIOLOGICAL AND OTHER TEST PROCEDURES USING LASER EXCITATION TECHNIQUES
US5565324A (en) 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5298741A (en) 1993-01-13 1994-03-29 Trustees Of Tufts College Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
CA2102884A1 (en) 1993-03-04 1994-09-05 James J. Wynne Dental procedures and apparatus using ultraviolet radiation
JP3302458B2 (en) 1993-08-31 2002-07-15 富士通株式会社 Integrated optical device and manufacturing method
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5494798A (en) 1993-12-09 1996-02-27 Gerdt; David W. Fiber optic evanscent wave sensor for immunoassay
ATE160223T1 (en) 1993-12-15 1997-11-15 Combichem Inc COMBINATORY LIBRARIES AND METHODS OF USE THEREOF
US5496997A (en) 1994-01-03 1996-03-05 Pope; Edward J. A. Sensor incorporating an optical fiber and a solid porous inorganic microsphere
CA2182517C (en) 1994-02-07 2001-08-21 Theo Nikiforov Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis
USH1531H (en) * 1994-04-18 1996-05-07 Blumentals; Ilse I. Thermophilic DNA polymerase
US5856083A (en) 1994-05-06 1999-01-05 Pharmacopeia, Inc. Lawn assay for compounds that affect enzyme activity or bind to target molecules
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
AU2990595A (en) 1994-07-26 1996-02-22 Sydney Brenner Multidimensional conduit combinatorial library synthesis device
US5512490A (en) 1994-08-11 1996-04-30 Trustees Of Tufts College Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6280935B1 (en) * 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US5795716A (en) * 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
WO1996030392A1 (en) 1995-03-28 1996-10-03 Novartis Ag Process for the production of combinatorial compound libraries
US5804376A (en) * 1995-05-02 1998-09-08 Incyte Pharmaceuticals, Inc. Pancreas-derived serpin
CA2221454A1 (en) 1995-05-19 1996-11-21 Abbott Laboratories Wide dynamic range nucleic acid detection using an aggregate primer series
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5690894A (en) 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5656241A (en) 1995-09-07 1997-08-12 Optical Sensors Incorporated Method for manufacturing fiber optic sensors
AU7398996A (en) 1995-10-11 1997-04-30 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
GB9521159D0 (en) 1995-10-16 1995-12-20 Brint Norman T A weapon
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5633972A (en) 1995-11-29 1997-05-27 Trustees Of Tufts College Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
EP2574617B1 (en) 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
JP3764779B2 (en) * 1996-03-30 2006-04-12 株式会社東北テクノアーチ Analysis method using convex regions
US5840256A (en) 1996-04-09 1998-11-24 David Sarnoff Research Center Inc. Plate for reaction system
US6251691B1 (en) 1996-04-25 2001-06-26 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
AU733782B2 (en) 1996-06-06 2001-05-24 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors
US5854684A (en) 1996-09-26 1998-12-29 Sarnoff Corporation Massively parallel detection
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
WO1998015644A2 (en) * 1996-09-27 1998-04-16 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6060245A (en) * 1996-12-13 2000-05-09 Stratagene Methods and adaptors for generating specific nucleic acid populations
US5976797A (en) 1996-12-20 1999-11-02 Hitachi Chemical Co., Ltd. Method for quantifying total mRNA with poly(A)-complementary oligonucleotide-immobilized microtiter plate
JP4663824B2 (en) 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド Multiplexed molecular analyzer and method
US20020042048A1 (en) 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
EP0975807B1 (en) 1997-05-02 2006-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6406845B1 (en) 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
AU736321B2 (en) 1997-05-23 2001-07-26 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
AU756945B2 (en) 1997-05-23 2003-01-30 Bioarray Solutions Ltd Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
US6096496A (en) * 1997-06-19 2000-08-01 Frankel; Robert D. Supports incorporating vertical cavity emitting lasers and tracking apparatus for use in combinatorial synthesis
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6287766B1 (en) * 1997-10-28 2001-09-11 The Regents Of The University Of California DNA polymorphism identity determination using flow cytometry
WO2000004193A1 (en) * 1998-07-20 2000-01-27 Yale University Method for detecting nucleic acids using target-mediated ligation of bipartite primers
US5998175A (en) 1998-07-24 1999-12-07 Lumigen, Inc. Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
US6306643B1 (en) 1998-08-24 2001-10-23 Affymetrix, Inc. Methods of using an array of pooled probes in genetic analysis
JP3855492B2 (en) * 1998-10-08 2006-12-13 株式会社日立製作所 Mixed nucleic acid fragment analysis
CA2356697C (en) * 1999-01-06 2010-06-22 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
ATE413467T1 (en) 1999-04-20 2008-11-15 Illumina Inc DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS
EP1179185B1 (en) 1999-05-07 2009-08-12 Life Technologies Corporation A method of detecting an analyte using semiconductor nanocrystals
US6372813B1 (en) * 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6251639B1 (en) 1999-09-13 2001-06-26 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
US6221603B1 (en) * 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
US6858412B2 (en) * 2000-10-24 2005-02-22 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic DNA

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
WO1997045559A1 (en) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
WO1998004746A1 (en) * 1996-07-31 1998-02-05 Mount Sinai School Of Medicine Of The City University Of New York Nucleic acid amplification method: ramification-extension amplification method (ram)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BANER J ET AL: "Signal amplification of padlock probes by rolling circle replication", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 22, 15 November 1998 (1998-11-15), pages 5073 - 5078, XP002112357, ISSN: 0305-1048 *
IANNONE MARIE A ET AL: "Multiplexed single nucleotide polymorphism genotyping by oligonucleotide ligation and flow cytometry.", CYTOMETRY, vol. 39, no. 2, 1 February 2000 (2000-02-01), pages 131 - 140, XP001073442, ISSN: 0196-4763 *
LIZARDI P M ET AL: "MUTATION DETECTION AND SINGLE-MOLECULE COUNTING USING ISOTHERMAL ROLLING-CIRCLE AMPLIFICATION", NATURE GENETICS, NEW YORK, NY, US, vol. 19, no. 3, July 1998 (1998-07-01), pages 225 - 232, XP000856939, ISSN: 1061-4036 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9399795B2 (en) 1998-06-24 2016-07-26 Illumina, Inc. Multiplex decoding of array sensors with microspheres
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

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