WO2001057248A3 - Polynucleotide arrays and their use in sequencing - Google Patents

Polynucleotide arrays and their use in sequencing Download PDF

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Publication number
WO2001057248A3
WO2001057248A3 PCT/GB2001/000407 GB0100407W WO0157248A3 WO 2001057248 A3 WO2001057248 A3 WO 2001057248A3 GB 0100407 W GB0100407 W GB 0100407W WO 0157248 A3 WO0157248 A3 WO 0157248A3
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WO
WIPO (PCT)
Prior art keywords
array
polynucleotide
molecules
sequencing
target
Prior art date
Application number
PCT/GB2001/000407
Other languages
French (fr)
Other versions
WO2001057248A2 (en
Inventor
Shankar Balasubramanian
David Bentley
Original Assignee
Solexa Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Solexa Ltd filed Critical Solexa Ltd
Priority to AU30384/01A priority Critical patent/AU3038401A/en
Priority to EP01902529A priority patent/EP1252332A2/en
Priority to JP2001555871A priority patent/JP2003521693A/en
Priority to CA002398168A priority patent/CA2398168A1/en
Priority to IL15083601A priority patent/IL150836A0/en
Publication of WO2001057248A2 publication Critical patent/WO2001057248A2/en
Publication of WO2001057248A3 publication Critical patent/WO2001057248A3/en
Priority to IS6489A priority patent/IS6489A/en
Priority to HK02109277.3A priority patent/HK1047771A1/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00675—In-situ synthesis on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00729—Peptide nucleic acids [PNA]

Abstract

A device for use in polynucleotide sequencing procedures comprises an array of polynucleotide molecules capable of interrogation and immobilised on a solid surface, wherein the array has a density which allows the molecules to be individually resolved. The molecules on the array comprise a target polynucleotide and a primer sequence, the primer being maintained in spatial relationship to the target, both being linked via a covalent bond. The device may be used in genotyping experiments, in which an array of genomic DNA is prepared to allow multiple sequencing reactions to be performed.
PCT/GB2001/000407 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing WO2001057248A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU30384/01A AU3038401A (en) 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing
EP01902529A EP1252332A2 (en) 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing
JP2001555871A JP2003521693A (en) 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing
CA002398168A CA2398168A1 (en) 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing
IL15083601A IL150836A0 (en) 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing
IS6489A IS6489A (en) 2000-02-01 2002-07-31 Multicore arrays and their use in sequencing
HK02109277.3A HK1047771A1 (en) 2000-02-01 2002-12-23 Polynucleotide arrays and their use in sequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0002310.1 2000-02-01
GBGB0002310.1A GB0002310D0 (en) 2000-02-01 2000-02-01 Polynucleotide sequencing

Publications (2)

Publication Number Publication Date
WO2001057248A2 WO2001057248A2 (en) 2001-08-09
WO2001057248A3 true WO2001057248A3 (en) 2002-02-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/000407 WO2001057248A2 (en) 2000-02-01 2001-01-31 Polynucleotide arrays and their use in sequencing

Country Status (9)

Country Link
EP (1) EP1252332A2 (en)
JP (1) JP2003521693A (en)
AU (1) AU3038401A (en)
CA (1) CA2398168A1 (en)
GB (1) GB0002310D0 (en)
HK (1) HK1047771A1 (en)
IL (1) IL150836A0 (en)
IS (1) IS6489A (en)
WO (1) WO2001057248A2 (en)

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