WO2001057222A1 - A protease, a gene therefor and the use thereof - Google Patents
A protease, a gene therefor and the use thereof Download PDFInfo
- Publication number
- WO2001057222A1 WO2001057222A1 PCT/IB2000/002058 IB0002058W WO0157222A1 WO 2001057222 A1 WO2001057222 A1 WO 2001057222A1 IB 0002058 W IB0002058 W IB 0002058W WO 0157222 A1 WO0157222 A1 WO 0157222A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protease
- enzyme
- agents
- gene
- mutant
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
Definitions
- an object of the present invention is to provide a novel gene coding for the protease comprising the sequence described in SEQ ID No. 2 and a mutant or a variant thereof which can produce said protease or its functional equivalents o
- Fig. 4 represents a DNA sequence coding for the protease of the invention and the corresponding amino acid sequence of the protein. Best Mode For Carrying Out The Invention
- mutant or a variant thereof is meant by a mutated gene in which a single base or two or more bases have been changed by a mutation such as, for example, substitution, deletion, addition or insertion, but still can produce the same protease or its functional equivalents. Therefore, those skilled in the art would easily appreciate that the mutant or variant can be encompassed within the scope of the present invention. Generally, a mutant or variant having about 80% or more of homology in the case of the protease and the gene of the invention, and preferably 90% or more of homology can be encompassed in the contexts of the invention, a mutant or a variant thereof.
- an enzyme variant or a mutant enzyme is meant by the functionally same enzyme as the protease in which a single amino acid or a plurality of the amino acids have been modified or changed due to the changes in DNA nucleotide sequence(s) of the parental gene or the derivatives thereof.
- preferable protease according to the invention can be derived from Aranicola proteolyticus, for example, Aranicola proteolyticus HY3-1
- the functionally equivalent protease can be obtained from a form of a variant or a mutant thereof using a vector system and a suitable host microorganism which may essentially not be the same organism from which the parental gene has been derived.
- protease is a zinc- binding metalloprotease requiring zinc ion(Zn 2+ ) for its activity.
- its relative activity was measured at different temperatures ranging from 4 °C to 80 °C, the maximum activity was observed at 37°C and at least 75% of relative activity was observed at temperatures between 20 °C to 40 °C (See Fig. 2).
- the protease according to the present invention has an apparent molecular weight of 51.5 lcDa and the optimal temperature for activity at 20°C to 37°C (at pH 7.6) and an optimal pH at 7.0 to 9.5 (at 37°C).
- the protease retains high activity under high salt concentrations and inhibited by metalloprotease inhibitors such as EDTA and phenanthroline.
- Aranicola proteolyticus HY-3 is a mobile aerobic Gram-negative bacterium. It is globular in shape with 0.5 to 0.8 mm in diameter. Its growth response was positive in the presence of catalase and negative in the presence of oxidase.
- compositions according to the invention comprise a pharmaceutically acceptable carrier in combination with the protease of the invention.
- Pharmaceutical formulations are well known and pharmaceutical compositions comprising the protease may be routinely formulated by one having ordinary skill in the art.
- the mode of administration of the protease may determine the sites in the organism to which the protease will be delivered.
- parenteral administration i.e., intravenous, subcutaneous, intramuscular.
- Intravenous administration is the preferred route. They are best used in the form of sterile aqueous solution which may contain other solutes, for example, sufficient salts, glucose or dextrose to make the solution isotonic.
- the concentration of the active ingredient, protease is about 10D ⁇ 100 D/D body weight per day.
- the dosage varies depending upon known factors such as the pharmacodynamic characteristics of the particular agent, and its mode and route of administration; age, health, and weight of the recipient; nature and extent of symptoms, kind of concurrent treatment, frequency of treatment, and the effect desired. See Gennaro, Alfonso, ed., Remington's Pharmaceutical Sciences, 18th Edition, 1990, Mack Publishing Co., Easton Pa.
- Aranicola proteolyticus HY-3 was cultured in a culture medium (Bactotryptone 0.5%, Yeast extract 0.5%o, NaCl 0.1%,
- Partial amino acid sequences of the protease obtained in the Example 1 were sequenced by an amino acid sequencing analyzer (Precise Protein Sequencing System, Applied Biosystems). Based on the amino acid sequence thus obtained as described by SEQ ID NO: 3 (Ala Glu Gin Gin Gin Gin Ala) and SEQ ID NO: 4(Ile Gly His Ala Leu Gly), PCR primers as described by SEQ ID NO: 5 (forward primer: gcggaacagc agcagcaggc) and SEQ ID NO: 6(reverse primer: gcccaacgca tggccaat) were designed. Using synthetic primers, PCR was performed in the presence of genomic DNA purified from Aranicola proteolyticus HY-3 as a template.
Abstract
Description
Claims
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR0011186-4A BR0011186A (en) | 2000-02-03 | 2000-12-29 | Protease, its gene and its uses |
AT00991301T ATE431402T1 (en) | 2000-02-03 | 2000-12-29 | PROTEASE, A GENE AND APPLICATION THEREOF |
AU32151/01A AU3215101A (en) | 2000-02-03 | 2000-12-29 | A protease, a gene therefor and the use thereof |
EP00991301A EP1165807B1 (en) | 2000-02-03 | 2000-12-29 | A protease, a gene therefor and the use thereof |
US09/958,321 US7297332B2 (en) | 2000-02-03 | 2000-12-29 | Protease, a gene therefor and the use thereof |
JP2001558036A JP4425515B2 (en) | 2000-02-03 | 2000-12-29 | Protease, gene for it and use thereof |
DE60042200T DE60042200D1 (en) | 2000-02-03 | 2000-12-29 | PROTEASE, A GENE FOR IT AND APPLICATION THEREOF |
US11/853,899 US7563872B2 (en) | 2000-02-03 | 2007-09-12 | Protease, a gene therefor and the use thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020000005479A KR20010077591A (en) | 2000-02-03 | 2000-02-03 | A novel metalloprotease and a gene thereof derived from Aranicola proteolyticus |
KR2000/5479 | 2000-02-03 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09958321 A-371-Of-International | 2000-12-29 | ||
US11/853,899 Continuation US7563872B2 (en) | 2000-02-03 | 2007-09-12 | Protease, a gene therefor and the use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2001057222A1 true WO2001057222A1 (en) | 2001-08-09 |
Family
ID=19644249
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2000/002058 WO2001057222A1 (en) | 2000-02-03 | 2000-12-29 | A protease, a gene therefor and the use thereof |
Country Status (10)
Country | Link |
---|---|
US (2) | US7297332B2 (en) |
EP (1) | EP1165807B1 (en) |
JP (1) | JP4425515B2 (en) |
KR (3) | KR20010077591A (en) |
CN (1) | CN100376679C (en) |
AT (1) | ATE431402T1 (en) |
AU (1) | AU3215101A (en) |
BR (1) | BR0011186A (en) |
DE (1) | DE60042200D1 (en) |
WO (1) | WO2001057222A1 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100909998B1 (en) | 2007-03-29 | 2009-07-29 | 주식회사 인섹트 바이오텍 | Pharmaceutical composition for breast cancer prevention and treatment with arazyme as an active ingredient |
EP2101809A1 (en) * | 2006-12-28 | 2009-09-23 | Insect Biotech Co., Ltd. | Pharmaceutical composition containing arazyme for the prevention of liver dysfunction |
EP2114431A1 (en) * | 2006-12-28 | 2009-11-11 | Insect Biotech Co., Ltd. | A composition containing arazyme for the prevention and treatment of cancer |
US20100278806A1 (en) * | 2008-01-11 | 2010-11-04 | Insect Biotech Co., Ltd. | Composition Containing Arazyme for the Prevention and Treatment of Arthritis |
US9492435B2 (en) | 2006-12-28 | 2016-11-15 | Infinity Pharmaceuticals, Inc. | Cyclopamine analogs |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20010077591A (en) * | 2000-02-03 | 2001-08-20 | 복성해 | A novel metalloprotease and a gene thereof derived from Aranicola proteolyticus |
KR100441377B1 (en) * | 2001-07-14 | 2004-07-23 | 주식회사 인섹트 바이오텍 | Method for preparation of leather using protease and method for treatment of wastes derived from leather production process using the same |
KR100426948B1 (en) * | 2002-01-23 | 2004-04-14 | 주식회사 인섹트 바이오텍 | Silk yarn scouring method using protease |
GB2433260A (en) * | 2005-12-16 | 2007-06-20 | Mologic Ltd | A selectable decarboxylase marker |
KR100811744B1 (en) * | 2006-12-28 | 2008-03-11 | 주식회사 인섹트 바이오텍 | Pharmaceutical composition containing arazyme for the prevention and treatment of cancer |
KR100836711B1 (en) * | 2006-12-28 | 2008-06-10 | 주식회사 인섹트 바이오텍 | Pharmaceutical composition containing arazyme for the prevention of liver dysfunction |
WO2012110562A2 (en) * | 2011-02-16 | 2012-08-23 | Novozymes A/S | Detergent compositions comprising metalloproteases |
KR101476399B1 (en) * | 2012-07-31 | 2014-12-24 | 주식회사 인섹트 바이오텍 | A composition containing Arazyme for the prevention and treatment of Atopic Dermatitis |
KR20160141581A (en) | 2015-06-01 | 2016-12-09 | 주식회사 네이처웍스 | Fermented additive for food, a korean sauce prepared by using the same and method for production of the korean sauce |
KR102083009B1 (en) * | 2015-06-05 | 2020-02-28 | 한국화학연구원 | Genetically modified Arazyme-producing microorganisms using ABC transporter-mediated secretion system and the method for preparing Arazyme using the same |
CN109295041A (en) * | 2018-10-10 | 2019-02-01 | 宁波希诺亚海洋生物科技有限公司 | With active polypeptide of serrapeptase and preparation method thereof |
KR102149232B1 (en) | 2018-12-20 | 2020-08-31 | 주식회사 아워홈 | Method for softening vegetable food material |
KR20200077691A (en) | 2018-12-20 | 2020-07-01 | 주식회사 아워홈 | Softening agent composition for vegetable food material |
CN114854645B (en) * | 2022-06-10 | 2023-07-07 | 陕西师范大学 | Application culture medium and culture method suitable for Serratia L-rhamnose induced expression system |
CN117187103A (en) * | 2023-06-25 | 2023-12-08 | 大庆亿莱检验检测技术服务有限公司 | Petroleum degrading bacterium and application thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR19980069206A (en) * | 1997-02-27 | 1998-10-26 | 박원훈 | Novel Microorganisms Isolated from the Intestines of Korean Sugarless Spiders and Proteolytic Enzymes Produced therefrom |
Family Cites Families (74)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3348992A (en) * | 1963-08-13 | 1967-10-24 | Madison Res & Dev Corp | Tufted products |
US3535178A (en) * | 1963-10-31 | 1970-10-20 | Bigelow Sanford Inc | Method of producing tufted pile fabric and nonwoven backing fabric for the same |
US3704191A (en) * | 1969-12-01 | 1972-11-28 | Francis M Buresh | Non-woven process |
US3819462A (en) * | 1970-10-12 | 1974-06-25 | Cotton Inc | Primary backing for tufted carpets |
US3950587A (en) * | 1971-01-12 | 1976-04-13 | Breveteam, S.A. | Non-woven textile fiber products having a relief-like structure |
US4001472A (en) * | 1971-09-03 | 1977-01-04 | Kimberly-Clark Corporation | Nonwoven reinforced cellulosic material and method of preparation |
US4131704A (en) * | 1976-01-02 | 1978-12-26 | Phillips Petroleum Company | Nonwoven fabric comprising needled and selectively fused fine and coarse filaments having differing softening temperatures which is useful as a backing in the production of tufted materials |
US4035533A (en) * | 1976-06-01 | 1977-07-12 | Champion International Corporation | Tufted carpet with meltable-film primary-backing component |
US4258094A (en) * | 1979-04-26 | 1981-03-24 | Brunswick Corporation | Melt bonded fabrics and a method for their production |
US4320167A (en) * | 1979-11-19 | 1982-03-16 | Phillips Petroleum Company | Nonwoven fabric and method of production thereof |
US4389443A (en) * | 1980-06-16 | 1983-06-21 | Ozite Corporation | Cut pile fabric with fused carrier and method of making same |
US4389442A (en) * | 1980-06-16 | 1983-06-21 | Ozite Corporation | Wall covering fabric with texturized loops |
US4379189A (en) * | 1980-12-19 | 1983-04-05 | Phillips Petroleum Company | Nonwoven textile fabric with fused face and raised loop pile |
GB2162213B (en) * | 1984-06-27 | 1987-06-17 | Spontex Sa | Improvements in and relating to cleaning |
US4931343A (en) * | 1985-07-31 | 1990-06-05 | Minnesota Mining And Manufacturing Company | Sheet material used to form portions of fasteners |
US4770917A (en) * | 1985-07-31 | 1988-09-13 | Minnesota Mining And Manufacturing Company | Sheet material used to form portions of fasteners |
JP2611206B2 (en) * | 1985-11-15 | 1997-05-21 | 武田薬品工業株式会社 | Genes and methods of using the same |
US4931544A (en) * | 1986-09-04 | 1990-06-05 | Cetus Corporation | Succinylated interleukin-2 for pharmaceutical compositions |
US5643397A (en) * | 1988-05-13 | 1997-07-01 | Minnesota Mining And Manufacturing Company | Equipment for forming a sheet of loop material |
US5080951A (en) * | 1989-08-03 | 1992-01-14 | Guthrie David W | Nonwoven fabric |
DE3926120A1 (en) * | 1989-08-08 | 1991-02-14 | Basf Ag | METHOD FOR PRODUCING FINE-PART POLYMER POWDER |
US5326612A (en) * | 1991-05-20 | 1994-07-05 | The Procter & Gamble Company | Nonwoven female component for refastenable fastening device and method of making the same |
ATE161400T1 (en) * | 1991-05-20 | 1998-01-15 | Procter & Gamble | MULTI-LAYER COMPONENT FOR A SURFACE ZIPPER |
ZA933072B (en) * | 1992-05-01 | 1994-10-30 | Hoechst Celanese Corp | A tufted fabric. |
US5783126A (en) * | 1992-08-11 | 1998-07-21 | E. Khashoggi Industries | Method for manufacturing articles having inorganically filled, starch-bound cellular matrix |
DE69305096T2 (en) * | 1993-01-07 | 1997-04-30 | Minnesota Mining & Mfg | FLEXIBLE NON-WOVEN |
US6093665A (en) * | 1993-09-30 | 2000-07-25 | Kimberly-Clark Worldwide, Inc. | Pattern bonded nonwoven fabrics |
US5669900A (en) * | 1993-11-03 | 1997-09-23 | Kimberly-Clark Worldwide, Inc. | Spunbond loop material for hook and loop fastening systems |
CA2120645C (en) * | 1993-12-21 | 2004-02-10 | Andrew Scott Burnes | Compressively resilient loop structure for hook and loop fastener systems |
US5814390A (en) * | 1995-06-30 | 1998-09-29 | Kimberly-Clark Worldwide, Inc. | Creased nonwoven web with stretch and recovery |
EP0765616B1 (en) * | 1995-09-28 | 2001-06-27 | Japan Vilene Company, Ltd. | Female member for face fastener and method of producing the same |
US5692949A (en) * | 1995-11-17 | 1997-12-02 | Minnesota Mining And Manufacturing Company | Back-up pad for use with abrasive articles |
US5614281A (en) * | 1995-11-29 | 1997-03-25 | Kimberly-Clark Corporation | Creped nonwoven laminate loop fastening material for mechanical fastening systems |
US5763041A (en) * | 1995-12-21 | 1998-06-09 | Kimberly-Clark Worldwide, Inc. | Laminate material |
US5858515A (en) * | 1995-12-29 | 1999-01-12 | Kimberly-Clark Worldwide, Inc. | Pattern-unbonded nonwoven web and process for making the same |
US5904793A (en) * | 1996-08-14 | 1999-05-18 | Minnesota Mining And Manufacturing Company | Method and equipment for rapid manufacture of loop material |
US6716511B2 (en) * | 1996-09-16 | 2004-04-06 | Bp Corporation North America Inc. | Propylene polymer fibers and yarns |
US5945215A (en) * | 1996-09-16 | 1999-08-31 | Bp Amoco Corporation | Propylene polymer fibers and yarns |
US5962112A (en) * | 1996-12-19 | 1999-10-05 | Kimberly-Clark Worldwide, Inc. | Wipers comprising point unbonded webs |
US5891547A (en) * | 1997-02-04 | 1999-04-06 | Precision Fabrics Group, Inc. | Needle punch nonwoven component for refastenable fastening device |
US5773120A (en) * | 1997-02-28 | 1998-06-30 | Kimberly-Clark Worldwide, Inc. | Loop material for hook-and-loop fastening system |
JP3877842B2 (en) * | 1997-03-05 | 2007-02-07 | ユニチカ株式会社 | Method for producing female material for hook-and-loop fastener |
US6265053B1 (en) * | 1998-03-13 | 2001-07-24 | Francis Joseph Kronzer | Printable material |
US5888607A (en) * | 1997-07-03 | 1999-03-30 | Minnesota Mining And Manufacturing Co. | Soft loop laminate and method of making |
US6869659B2 (en) * | 1997-09-03 | 2005-03-22 | Velcro Industries B.V. | Fastener loop material, its manufacture, and products incorporating the material |
US6329016B1 (en) * | 1997-09-03 | 2001-12-11 | Velcro Industries B.V. | Loop material for touch fastening |
US6342285B1 (en) * | 1997-09-03 | 2002-01-29 | Velcro Industries B.V. | Fastener loop material, its manufacture, and products incorporating the material |
US5997981A (en) * | 1997-09-15 | 1999-12-07 | Kimberly-Clark Worldwide, Inc. | Breathable barrier composite useful as an ideal loop fastener component |
US5964742A (en) * | 1997-09-15 | 1999-10-12 | Kimberly-Clark Worldwide, Inc. | Nonwoven bonding patterns producing fabrics with improved strength and abrasion resistance |
US6410138B2 (en) * | 1997-09-30 | 2002-06-25 | Kimberly-Clark Worldwide, Inc. | Crimped multicomponent filaments and spunbond webs made therefrom |
JP4008136B2 (en) * | 1998-02-23 | 2007-11-14 | 日本バイリーン株式会社 | Hook and loop fastener female material and manufacturing method thereof |
US6086984A (en) * | 1998-05-22 | 2000-07-11 | Delaware Valley Corporation | Elastic nonwoven fabric |
US6162522A (en) * | 1998-06-19 | 2000-12-19 | Kimberly-Clark Worldwide, Inc. | Loop substrate for releasably attachable abrasive sheet material |
US6454989B1 (en) * | 1998-11-12 | 2002-09-24 | Kimberly-Clark Worldwide, Inc. | Process of making a crimped multicomponent fiber web |
JP3408984B2 (en) * | 1999-01-28 | 2003-05-19 | パナソニック コミュニケーションズ株式会社 | Network facsimile machine |
KR20010077591A (en) * | 2000-02-03 | 2001-08-20 | 복성해 | A novel metalloprotease and a gene thereof derived from Aranicola proteolyticus |
US6756327B2 (en) * | 2000-10-31 | 2004-06-29 | Kimberly-Clark Worldwide, Inc. | Loop fastening component made from thermally retracted materials |
US6489004B1 (en) * | 2000-11-03 | 2002-12-03 | Kimberly-Clark Worldwide, Inc. | Hook and loop fastener having an increased coefficient of friction |
US6638611B2 (en) * | 2001-02-09 | 2003-10-28 | 3M Innovative Properties Company | Multipurpose cosmetic wipes |
EP1233421B1 (en) * | 2001-02-19 | 2007-07-11 | STMicroelectronics S.r.l. | Method for refreshing stored data in an electrically erasable and programmable non-volatile memory |
US6740385B2 (en) * | 2001-03-28 | 2004-05-25 | Bp Corporation North America Inc. | Tuftable and tufted fabrics |
CA2450380A1 (en) * | 2001-06-12 | 2002-12-19 | Velcro Industries B.V. | Loop materials for touch fastening |
US6921570B2 (en) * | 2001-12-21 | 2005-07-26 | Kimberly-Clark Worldwide, Inc. | Pattern unbonded nonwoven web and process for making same |
US20030232170A1 (en) * | 2002-06-12 | 2003-12-18 | Gillette Samuel Mark | Spunlaced loop material for a refastenable fastening device and methods of making same |
US20050196583A1 (en) * | 2002-12-03 | 2005-09-08 | Provost George A. | Embossing loop materials |
US20050217092A1 (en) * | 2002-12-03 | 2005-10-06 | Barker James R | Anchoring loops of fibers needled into a carrier sheet |
US7547469B2 (en) * | 2002-12-03 | 2009-06-16 | Velcro Industries B.V. | Forming loop materials |
AU2003298847A1 (en) * | 2002-12-03 | 2004-06-23 | Velcro Industries B.V. | Needling through carrier sheets to form loops |
US7622408B2 (en) * | 2003-07-01 | 2009-11-24 | Dzs, Llc | Fabric-faced composites and methods for making same |
US7497978B2 (en) * | 2003-07-01 | 2009-03-03 | Dzs, Llc. | Process for abrasion-resistant needle-punched composite |
US7562426B2 (en) * | 2005-04-08 | 2009-07-21 | Velcro Industries B.V. | Needling loops into carrier sheets |
US20070178273A1 (en) * | 2006-02-01 | 2007-08-02 | Provost George A | Embossing loop materials |
US20080113152A1 (en) * | 2006-11-14 | 2008-05-15 | Velcro Industries B.V. | Loop Materials |
KR100811744B1 (en) | 2006-12-28 | 2008-03-11 | 주식회사 인섹트 바이오텍 | Pharmaceutical composition containing arazyme for the prevention and treatment of cancer |
-
2000
- 2000-02-03 KR KR1020000005479A patent/KR20010077591A/en active Search and Examination
- 2000-12-29 DE DE60042200T patent/DE60042200D1/en not_active Expired - Lifetime
- 2000-12-29 JP JP2001558036A patent/JP4425515B2/en not_active Expired - Fee Related
- 2000-12-29 KR KR1020017012239A patent/KR100836184B1/en active IP Right Grant
- 2000-12-29 WO PCT/IB2000/002058 patent/WO2001057222A1/en active Application Filing
- 2000-12-29 AT AT00991301T patent/ATE431402T1/en not_active IP Right Cessation
- 2000-12-29 AU AU32151/01A patent/AU3215101A/en not_active Abandoned
- 2000-12-29 BR BR0011186-4A patent/BR0011186A/en not_active Application Discontinuation
- 2000-12-29 US US09/958,321 patent/US7297332B2/en not_active Expired - Lifetime
- 2000-12-29 KR KR1020087005844A patent/KR100879962B1/en active IP Right Grant
- 2000-12-29 EP EP00991301A patent/EP1165807B1/en not_active Expired - Lifetime
- 2000-12-29 CN CNB00805665XA patent/CN100376679C/en not_active Expired - Lifetime
-
2007
- 2007-09-12 US US11/853,899 patent/US7563872B2/en not_active Expired - Lifetime
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR19980069206A (en) * | 1997-02-27 | 1998-10-26 | 박원훈 | Novel Microorganisms Isolated from the Intestines of Korean Sugarless Spiders and Proteolytic Enzymes Produced therefrom |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2101809A1 (en) * | 2006-12-28 | 2009-09-23 | Insect Biotech Co., Ltd. | Pharmaceutical composition containing arazyme for the prevention of liver dysfunction |
EP2114431A1 (en) * | 2006-12-28 | 2009-11-11 | Insect Biotech Co., Ltd. | A composition containing arazyme for the prevention and treatment of cancer |
EP2114431A4 (en) * | 2006-12-28 | 2011-03-16 | Insect Biotech Co Ltd | A composition containing arazyme for the prevention and treatment of cancer |
EP2101809A4 (en) * | 2006-12-28 | 2011-03-16 | Insect Biotech Co Ltd | Pharmaceutical composition containing arazyme for the prevention of liver dysfunction |
US8367060B2 (en) | 2006-12-28 | 2013-02-05 | Insect Biotech Co., Ltd. | Pharmaceutical composition containing arazyme for the prevention of liver dysfunction |
US8399223B2 (en) | 2006-12-28 | 2013-03-19 | Inssect Biotech Co., Ltd. | Composition containing arazyme for the prevention and treatment of cancer |
US9492435B2 (en) | 2006-12-28 | 2016-11-15 | Infinity Pharmaceuticals, Inc. | Cyclopamine analogs |
KR100909998B1 (en) | 2007-03-29 | 2009-07-29 | 주식회사 인섹트 바이오텍 | Pharmaceutical composition for breast cancer prevention and treatment with arazyme as an active ingredient |
US20100278806A1 (en) * | 2008-01-11 | 2010-11-04 | Insect Biotech Co., Ltd. | Composition Containing Arazyme for the Prevention and Treatment of Arthritis |
Also Published As
Publication number | Publication date |
---|---|
CN100376679C (en) | 2008-03-26 |
AU3215101A (en) | 2001-08-14 |
KR20010077591A (en) | 2001-08-20 |
BR0011186A (en) | 2002-03-05 |
EP1165807A4 (en) | 2004-04-14 |
JP4425515B2 (en) | 2010-03-03 |
KR20010112928A (en) | 2001-12-22 |
EP1165807A1 (en) | 2002-01-02 |
DE60042200D1 (en) | 2009-06-25 |
US20080069846A1 (en) | 2008-03-20 |
KR20080027972A (en) | 2008-03-28 |
US7297332B2 (en) | 2007-11-20 |
KR100879962B1 (en) | 2009-01-23 |
US7563872B2 (en) | 2009-07-21 |
KR100836184B1 (en) | 2008-06-09 |
ATE431402T1 (en) | 2009-05-15 |
US20040162417A1 (en) | 2004-08-19 |
CN1345376A (en) | 2002-04-17 |
EP1165807B1 (en) | 2009-05-13 |
JP2003521926A (en) | 2003-07-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7563872B2 (en) | Protease, a gene therefor and the use thereof | |
EP1456366B1 (en) | Novel alkali protease formed by bacillus gibsonii (dsm 14393) and washing and cleaning agents containing said novel alkali protease | |
EP1456368B1 (en) | Novel alkaline protease from bacillus sp. (dsm 14392) and washing and cleaning products comprising said novel alkaline protease | |
Izotova et al. | Purification and properties of serine protease from Halobacterium halobium | |
JP2690339B2 (en) | New protease | |
Teufel et al. | Characterization of an extracellular metalloprotease with elastase activity from Staphylococcus epidermidis | |
Mitsuiki et al. | Molecular characterization of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1 | |
US4002572A (en) | Alkaline protease produced by a bacillus | |
DE10153792A1 (en) | New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants | |
EP0482879B1 (en) | Protease from Bacillus licheniformis | |
Oleksy et al. | Growth phase-dependent production of a cell wall-associated elastinolytic cysteine proteinase by Staphylococcus epidermidis | |
DE10328887B4 (en) | Alkaline protease | |
JP4137526B2 (en) | Keratinase and production method thereof | |
Öztürk et al. | Alkaline serine protease from halotolerant Bacillus licheniformis BA17 | |
WO2006054595A1 (en) | Novel high alkaline protease and use thereof | |
Jankiewicz et al. | Biochemical characterization of an alkaline metallopeptidase secreted by a Pseudomonas fluorescens isolated from soil | |
JP3664804B2 (en) | Low-temperature alkaline protease X, microorganism producing the same, method for producing the same, detergent composition containing the enzyme, and enzyme preparation for food processing | |
JPH02255087A (en) | Heat-resistant alkali protease and bacillus sp. no. ah-101 strain | |
JP3664802B2 (en) | Low-temperature alkaline protease, microorganism producing the same, method for producing the same, detergent composition containing the enzyme, and enzyme preparation for food processing | |
JP3664801B2 (en) | Low temperature alkaline protease S, microorganism producing the same, method for producing the same, detergent composition containing the enzyme, and enzyme preparation for food processing | |
Kim et al. | Gene cloning and characterization of a Bacillus vietnamensis metalloprotease | |
JP3592795B2 (en) | Low-temperature optimal alkaline protease, microorganism producing the same, and method for producing the alkaline protease | |
KR0145485B1 (en) | Thermoactinomyces sp.e79 and new proteolytic enzyme produced therefrom | |
JP3664800B2 (en) | Low-temperature alkaline protease T15, microorganism producing the same, method for producing the same, detergent composition containing the enzyme, and enzyme preparation for food processing | |
Lee et al. | Production and Characterization of ans Alkaline Protease from an Isolate, Xanthomonas sp. YL-37 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 00805665.X Country of ref document: CN |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1020017012239 Country of ref document: KR |
|
WWE | Wipo information: entry into national phase |
Ref document number: IN/PCT/2001/1006/KOL Country of ref document: IN |
|
ENP | Entry into the national phase |
Ref document number: 2001 558036 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09958321 Country of ref document: US |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2000991301 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 2000991301 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |