WO2001029252A3 - A method for the generation of repeat-depleted dna - Google Patents

A method for the generation of repeat-depleted dna Download PDF

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Publication number
WO2001029252A3
WO2001029252A3 PCT/EP2000/010268 EP0010268W WO0129252A3 WO 2001029252 A3 WO2001029252 A3 WO 2001029252A3 EP 0010268 W EP0010268 W EP 0010268W WO 0129252 A3 WO0129252 A3 WO 0129252A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
repeat
generation
pcr
depleted
Prior art date
Application number
PCT/EP2000/010268
Other languages
French (fr)
Other versions
WO2001029252A2 (en
Inventor
Michael Speicher
Roland Eils
Original Assignee
Michael Speicher
Roland Eils
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michael Speicher, Roland Eils filed Critical Michael Speicher
Priority to AU77890/00A priority Critical patent/AU7789000A/en
Publication of WO2001029252A2 publication Critical patent/WO2001029252A2/en
Publication of WO2001029252A3 publication Critical patent/WO2001029252A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

The invention relates to a method for the generation of repeat-depleted DNA comprising amplifying repetitive template DNA by a first polymerase chain reaction (PCR), wherein the hybridization step is a low stringency hybidization step and a second PCR followi ng the first PCR, wherein the hybridization step of said second PCR is a high stringency hybridization step. The repeat-depleted DNA obtained can be used as probe or cloned into vectors, plasmid, etc. Further, the invention relates to the application of the method in the generation of and hybridization with DNA libraries, DNA arrays or DNA blots.
PCT/EP2000/010268 1999-10-18 2000-10-18 A method for the generation of repeat-depleted dna WO2001029252A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU77890/00A AU7789000A (en) 1999-10-18 2000-10-18 A method for the generation of repeat-depleted dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99120618.6 1999-10-18
EP99120618 1999-10-18

Publications (2)

Publication Number Publication Date
WO2001029252A2 WO2001029252A2 (en) 2001-04-26
WO2001029252A3 true WO2001029252A3 (en) 2002-01-31

Family

ID=8239219

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010268 WO2001029252A2 (en) 1999-10-18 2000-10-18 A method for the generation of repeat-depleted dna

Country Status (2)

Country Link
AU (1) AU7789000A (en)
WO (1) WO2001029252A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003901671A0 (en) * 2003-04-02 2003-05-01 The University Of Adelaide Comparative genomic hybridization
CN106222250A (en) * 2016-07-22 2016-12-14 厦门艾德生物医药科技股份有限公司 A kind of preparation method of FISH probe

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5824479A (en) * 1995-05-22 1998-10-20 Forschungszentrum Fur, Umwelt Und Gesundheit Gmbh Inter-line-PCR
US5861245A (en) * 1990-10-15 1999-01-19 Stratagene & California Institute Of Biological Research Arbitrarily primed polymerase chain reaction method for fingerprinting genomes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861245A (en) * 1990-10-15 1999-01-19 Stratagene & California Institute Of Biological Research Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5824479A (en) * 1995-05-22 1998-10-20 Forschungszentrum Fur, Umwelt Und Gesundheit Gmbh Inter-line-PCR

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOLZER A ET AL: "COMPLETE SET OF REPEAT-DEPLETED, PCR-AMPLIFIABLE, HUMAN CHROMOSOME-SPECIFIC PAINTING PROBES", CYTOGENETICS AND CELL GENETICS,CH,BASEL, vol. 84, no. 3/04, 1999, pages 233 - 240, XP000907217, ISSN: 0301-0171 *
CHEN-LIU L W ET AL: "SELECTION OF HYBRIDS BY AFFINITY CAPTURE (SHAC): A METHOD FOR THE GENERATION OF CDNAS ENRICHED IN SEQUENCES FORM A SPECIFIC CHROMOSOME REGION", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 30, no. 2, 20 November 1995 (1995-11-20), pages 388 - 392, XP002051194, ISSN: 0888-7543 *
CRAIG J M ET AL: "REMOVAL OF REPETITIVE SEQUENCES FROM FISH PROBES USING PCR-ASSISTEDAFFINITY CHROMATOGRAPHY", HUMAN GENETICS,DE,BERLIN, vol. 100, no. 3/04, 1 September 1997 (1997-09-01), pages 472 - 476, XP002051198 *
MELTZER P S ET AL: "RAPID GENERATION OF REGION SPECIFIC PROBES BY CHROMOSOME MICRODISSECTION AND THEIR APPLICATION", NATURE GENETICS,US,NEW YORK, NY, vol. 1, 1 April 1992 (1992-04-01), pages 24 - 28, XP000400731, ISSN: 1061-4036 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 13, 1992, pages 718 - 725, XP000199116, ISSN: 0888-7543 *

Also Published As

Publication number Publication date
AU7789000A (en) 2001-04-30
WO2001029252A2 (en) 2001-04-26

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