WO2001027326A3 - Template-dependent ligation with pna-dna chimeric probes - Google Patents

Template-dependent ligation with pna-dna chimeric probes Download PDF

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Publication number
WO2001027326A3
WO2001027326A3 PCT/US2000/027730 US0027730W WO0127326A3 WO 2001027326 A3 WO2001027326 A3 WO 2001027326A3 US 0027730 W US0027730 W US 0027730W WO 0127326 A3 WO0127326 A3 WO 0127326A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligation
pna
template
dna chimeric
chimeric probes
Prior art date
Application number
PCT/US2000/027730
Other languages
French (fr)
Other versions
WO2001027326A2 (en
Inventor
Michael Egholm
Caifu Chen
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Priority to EP00968853A priority Critical patent/EP1220953B1/en
Priority to AT00968853T priority patent/ATE285479T1/en
Priority to DK00968853T priority patent/DK1220953T3/en
Priority to DE60016947T priority patent/DE60016947T2/en
Priority to AU78712/00A priority patent/AU768240B2/en
Priority to JP2001529456A priority patent/JP3789817B2/en
Priority to CA002353801A priority patent/CA2353801A1/en
Publication of WO2001027326A2 publication Critical patent/WO2001027326A2/en
Publication of WO2001027326A3 publication Critical patent/WO2001027326A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Abstract

The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3' hydroxyl or 5' hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.
PCT/US2000/027730 1999-10-08 2000-10-06 Template-dependent ligation with pna-dna chimeric probes WO2001027326A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP00968853A EP1220953B1 (en) 1999-10-08 2000-10-06 Template-dependent ligation with pna-dna chimeric probes
AT00968853T ATE285479T1 (en) 1999-10-08 2000-10-06 TEMPLATE DEPENDENT LIGATION WITH PNA-DNA CHIMERIC PROBE
DK00968853T DK1220953T3 (en) 1999-10-08 2000-10-06 Template-dependent ligation with PNA-DNA chimeric probes
DE60016947T DE60016947T2 (en) 1999-10-08 2000-10-06 Template Dependent ligation with PNA-DNA chimeric probes
AU78712/00A AU768240B2 (en) 1999-10-08 2000-10-06 Template-dependent ligation with PNA-DNA chimeric probes
JP2001529456A JP3789817B2 (en) 1999-10-08 2000-10-06 Template-dependent ligation using PNA-DNA chimeric probes
CA002353801A CA2353801A1 (en) 1999-10-08 2000-10-06 Template-dependent ligation with pna-dna chimeric probes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/416,003 1999-10-08
US09/416,003 US6297016B1 (en) 1999-10-08 1999-10-08 Template-dependent ligation with PNA-DNA chimeric probes

Publications (2)

Publication Number Publication Date
WO2001027326A2 WO2001027326A2 (en) 2001-04-19
WO2001027326A3 true WO2001027326A3 (en) 2002-05-10

Family

ID=23648121

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/027730 WO2001027326A2 (en) 1999-10-08 2000-10-06 Template-dependent ligation with pna-dna chimeric probes

Country Status (8)

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US (2) US6297016B1 (en)
EP (1) EP1220953B1 (en)
JP (2) JP3789817B2 (en)
AT (1) ATE285479T1 (en)
AU (1) AU768240B2 (en)
CA (1) CA2353801A1 (en)
DE (1) DE60016947T2 (en)
WO (1) WO2001027326A2 (en)

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US8652780B2 (en) 2007-03-26 2014-02-18 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses

Also Published As

Publication number Publication date
JP3789817B2 (en) 2006-06-28
DE60016947D1 (en) 2005-01-27
EP1220953A2 (en) 2002-07-10
AU7871200A (en) 2001-04-23
ATE285479T1 (en) 2005-01-15
US6297016B1 (en) 2001-10-02
EP1220953B1 (en) 2004-12-22
JP2003511059A (en) 2003-03-25
US20020177133A1 (en) 2002-11-28
WO2001027326A2 (en) 2001-04-19
AU768240B2 (en) 2003-12-04
JP2006055177A (en) 2006-03-02
CA2353801A1 (en) 2001-04-19
US6469151B1 (en) 2002-10-22
DE60016947T2 (en) 2005-12-15

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