WO2001025779A3 - Electrophoresis device for analysing labelled molecules, in particular, biological molecules - Google Patents

Electrophoresis device for analysing labelled molecules, in particular, biological molecules Download PDF

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Publication number
WO2001025779A3
WO2001025779A3 PCT/EP2000/009777 EP0009777W WO0125779A3 WO 2001025779 A3 WO2001025779 A3 WO 2001025779A3 EP 0009777 W EP0009777 W EP 0009777W WO 0125779 A3 WO0125779 A3 WO 0125779A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
another
analysing
fluorescent markers
balanced
Prior art date
Application number
PCT/EP2000/009777
Other languages
German (de)
French (fr)
Other versions
WO2001025779A2 (en
Inventor
Wilhelm Ansorge
Josef Stegemann
Robert Ventzki
Christian Schwager
Original Assignee
Europ Lab Molekularbiolog
Wilhelm Ansorge
Josef Stegemann
Robert Ventzki
Christian Schwager
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europ Lab Molekularbiolog, Wilhelm Ansorge, Josef Stegemann, Robert Ventzki, Christian Schwager filed Critical Europ Lab Molekularbiolog
Priority to AU10219/01A priority Critical patent/AU1021901A/en
Publication of WO2001025779A2 publication Critical patent/WO2001025779A2/en
Publication of WO2001025779A3 publication Critical patent/WO2001025779A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules

Abstract

The invention relates to an electrophoresis device (10) for analysing molecules labelled with fluorescent markers, in particular, biological molecules. According to one aspect of the invention, said device is suitable for resolving at least four different fluorescent markers independently of one another using a detector field assigned to each marker, according to the respective gel electrophoresis lane and the respective fluorescent marker. To this end, spectral filters are balanced with one another with regard to their spectral selectivity, the fluorescent markers are balanced with one another with regard to their spectral absorption and emission selectivity and the wavelengths of the laser beams (60, 62, 64, 66) which are used to excite the fluorescent markers are balanced with one another in a corresponding manner.
PCT/EP2000/009777 1999-10-07 2000-10-05 Electrophoresis device for analysing labelled molecules, in particular, biological molecules WO2001025779A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU10219/01A AU1021901A (en) 1999-10-07 2000-10-05 Electrophoresis device for analysing labelled molecules, in particular, biological molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19948391.4 1999-10-07
DE1999148391 DE19948391A1 (en) 1999-10-07 1999-10-07 Electrophoresis device for analyzing labeled molecules, especially biological molecules

Publications (2)

Publication Number Publication Date
WO2001025779A2 WO2001025779A2 (en) 2001-04-12
WO2001025779A3 true WO2001025779A3 (en) 2002-02-28

Family

ID=7924860

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/009777 WO2001025779A2 (en) 1999-10-07 2000-10-05 Electrophoresis device for analysing labelled molecules, in particular, biological molecules

Country Status (3)

Country Link
AU (1) AU1021901A (en)
DE (1) DE19948391A1 (en)
WO (1) WO2001025779A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004015488B4 (en) 2004-03-26 2008-01-31 Lavision Biotec Gmbh Method for reading out by strip-scanning of flat objects with substances that emit fluorescence radiation
DE102005022880B4 (en) * 2005-05-18 2010-12-30 Olympus Soft Imaging Solutions Gmbh Separation of spectrally or color superimposed image contributions in a multi-color image, especially in transmission microscopic multi-color images
DE102008034008B4 (en) 2008-07-21 2010-07-01 Carl Zeiss Surgical Gmbh Filter kit for the observation of fluorescence radiation in biological tissue
DE102008052223A1 (en) * 2008-10-17 2010-04-22 Albert-Ludwigs-Universität Freiburg Luminescence scanner for spatially resolved determination of physical characteristics of semiconductor component, has analyzing unit determining physical properties of semiconductor component from detected spatially resolved luminescence
DE102016118898B4 (en) 2016-10-05 2022-03-17 Ferdinand-Braun-Institut gGmbH, Leibniz- Institut für Höchstfrequenztechnik Optical system and method for spectroscopy
CN109270004B (en) * 2018-10-11 2023-09-12 南京信息工程大学 Atmospheric analysis detection system and detection method based on DOAS and LIBS technologies

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3046729A1 (en) * 1980-12-11 1982-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg THERMOSTATIC PLATE FOR AN ELECTROPHORESE DEVICE
EP0251306A1 (en) * 1986-07-04 1988-01-07 DIAGEN Institut für molekularbiologische Diagnostik GmbH Process and apparatus for the preparation of a controllable and reproduceable temperature gradient, and its use
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
DE19500638A1 (en) * 1994-01-14 1995-07-20 Hitachi Ltd Electrophoresis appts.
WO1996012948A1 (en) * 1994-10-20 1996-05-02 Pharmacia Biotech Ab Method and arrangement in a gel electrophoresis apparatus
EP0723149A2 (en) * 1995-01-19 1996-07-24 Hitachi, Ltd. Capillary array electrophoresis system
WO1996023213A1 (en) * 1995-01-23 1996-08-01 Murray Anthony J Analysis of biological molecules
EP0911630A1 (en) * 1997-08-07 1999-04-28 Hitachi Electronics Engineering Co., Ltd. Fluorescence detector

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3046729A1 (en) * 1980-12-11 1982-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg THERMOSTATIC PLATE FOR AN ELECTROPHORESE DEVICE
EP0251306A1 (en) * 1986-07-04 1988-01-07 DIAGEN Institut für molekularbiologische Diagnostik GmbH Process and apparatus for the preparation of a controllable and reproduceable temperature gradient, and its use
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
DE19500638A1 (en) * 1994-01-14 1995-07-20 Hitachi Ltd Electrophoresis appts.
WO1996012948A1 (en) * 1994-10-20 1996-05-02 Pharmacia Biotech Ab Method and arrangement in a gel electrophoresis apparatus
EP0723149A2 (en) * 1995-01-19 1996-07-24 Hitachi, Ltd. Capillary array electrophoresis system
WO1996023213A1 (en) * 1995-01-23 1996-08-01 Murray Anthony J Analysis of biological molecules
EP0911630A1 (en) * 1997-08-07 1999-04-28 Hitachi Electronics Engineering Co., Ltd. Fluorescence detector

Also Published As

Publication number Publication date
DE19948391A1 (en) 2001-04-12
WO2001025779A2 (en) 2001-04-12
AU1021901A (en) 2001-05-10

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