WO2001021635A3 - Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events - Google Patents

Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events Download PDF

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Publication number
WO2001021635A3
WO2001021635A3 PCT/DE2000/003016 DE0003016W WO0121635A3 WO 2001021635 A3 WO2001021635 A3 WO 2001021635A3 DE 0003016 W DE0003016 W DE 0003016W WO 0121635 A3 WO0121635 A3 WO 0121635A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
specific nucleic
hybridisation
oligomer
detecting sequence
Prior art date
Application number
PCT/DE2000/003016
Other languages
German (de)
French (fr)
Other versions
WO2001021635A2 (en
Inventor
Gerhard Hartwich
Original Assignee
Friz Biochem Gmbh
Gerhard Hartwich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Friz Biochem Gmbh, Gerhard Hartwich filed Critical Friz Biochem Gmbh
Priority to EP00972570A priority Critical patent/EP1228081A1/en
Priority to AU11285/01A priority patent/AU1128501A/en
Publication of WO2001021635A2 publication Critical patent/WO2001021635A2/en
Publication of WO2001021635A3 publication Critical patent/WO2001021635A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The present invention relates to a method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events. DNA-/RNA-/PNA oligomer single strands which are bound to a conductive surface at one end and are linked to a catalytic redoxactive unit at the remaining, free end serve as a hybridisation matrix (probe). A proportion of the single strand oligonucleotides are hybridised by means of a treatment with the oligonucleotide solution (target) that is to be examined. The electric communication between the conductive surface and the catalytic redoxactive unit, which is initially non- or barely existent, is increased. A hybridisation event can thus be detected using electrochemical methods such as voltametry, amperometry, potentiometry or conductivity measurement.
PCT/DE2000/003016 1999-09-22 2000-09-01 Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events WO2001021635A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00972570A EP1228081A1 (en) 1999-09-22 2000-09-01 Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events
AU11285/01A AU1128501A (en) 1999-09-22 2000-09-01 Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19945398A DE19945398A1 (en) 1999-09-22 1999-09-22 Electrochemical detection of sequence specific nucleic acid oligomer hybridization events
DE19945398.5 1999-09-22

Publications (2)

Publication Number Publication Date
WO2001021635A2 WO2001021635A2 (en) 2001-03-29
WO2001021635A3 true WO2001021635A3 (en) 2001-05-17

Family

ID=7922897

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/003016 WO2001021635A2 (en) 1999-09-22 2000-09-01 Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events

Country Status (4)

Country Link
EP (1) EP1228081A1 (en)
AU (1) AU1128501A (en)
DE (1) DE19945398A1 (en)
WO (1) WO2001021635A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6649350B2 (en) * 1997-04-09 2003-11-18 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
DE10013254A1 (en) 2000-03-17 2001-10-04 Friz Biochem Gmbh New apparatus, useful for detecting biomolecules and polymers, comprises array of samples containing specific molecules with separate light sources and system for detecting which samples interact with molecule to be detected
DE10049527A1 (en) * 2000-10-06 2001-09-06 Friz Biochem Gmbh A new electrochemical nucleic acid probe comprises an oligonucleotide bound to a thermostable photosynthetic reaction center is useful to detect hybridized nucleic acids
DE102005007148B4 (en) * 2005-02-11 2008-05-15 Universität Rostock Method and array for replicating and analyzing nucleic acids
US20090227691A1 (en) * 2007-11-07 2009-09-10 Riken Method for releasing molecule of interest based on target nucleic acid sequence
DE102013000682B3 (en) * 2013-01-08 2013-10-31 Rainer Hintsche Detecting molecules, involves immobilizing mixtures of two or more different biochemical capture molecules on two or more analytical array positions, and binding two or more different analytes on each array position

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US5770369A (en) * 1993-12-10 1998-06-23 California Institute Of Technology Nucleic acid mediated electron transfer
DE19901761A1 (en) * 1999-01-18 1999-07-01 Gerhard Dr Hartwich Oligonucleotides tagged with photoinducible redox-active unit
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO2000031101A1 (en) * 1998-11-23 2000-06-02 Friz Biochem Gmbh Method for the electrochemical detection of nucleic acid oligomer hybrids

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770369A (en) * 1993-12-10 1998-06-23 California Institute Of Technology Nucleic acid mediated electron transfer
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO2000031101A1 (en) * 1998-11-23 2000-06-02 Friz Biochem Gmbh Method for the electrochemical detection of nucleic acid oligomer hybrids
DE19901761A1 (en) * 1999-01-18 1999-07-01 Gerhard Dr Hartwich Oligonucleotides tagged with photoinducible redox-active unit

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WILLNER I ET AL: "ASSEMBLY OF FUNCTIONALIZED MONOLAYERS OF REDOX PROTEINS ON ELECTRODE SURFACES: NOVEL BIOELECTRONIC AND OPTOBIOELECTRONIC SYSTEMS", BIOSENSORS & BIOELECTRONICS,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 12, no. 4, 1997, pages 337 - 356, XP000874626, ISSN: 0956-5663 *

Also Published As

Publication number Publication date
EP1228081A1 (en) 2002-08-07
WO2001021635A2 (en) 2001-03-29
DE19945398A1 (en) 2001-04-05
AU1128501A (en) 2001-04-24

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