WO2000079241A3 - A system for cell-based screening - Google Patents

A system for cell-based screening Download PDF

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Publication number
WO2000079241A3
WO2000079241A3 PCT/US2000/040260 US0040260W WO0079241A3 WO 2000079241 A3 WO2000079241 A3 WO 2000079241A3 US 0040260 W US0040260 W US 0040260W WO 0079241 A3 WO0079241 A3 WO 0079241A3
Authority
WO
WIPO (PCT)
Prior art keywords
macromolecule
trafficking
digital data
test compound
cell
Prior art date
Application number
PCT/US2000/040260
Other languages
French (fr)
Other versions
WO2000079241A2 (en
Inventor
Richard A Rubin
Albert H Gough
Richik N Ghosh
Kenneth A Giuliano
R Terry Dunlay
Bruce Conway
Original Assignee
Cellomics Inc
Richard A Rubin
Albert H Gough
Richik N Ghosh
Kenneth A Giuliano
R Terry Dunlay
Bruce Conway
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/352,171 external-priority patent/US6759206B1/en
Application filed by Cellomics Inc, Richard A Rubin, Albert H Gough, Richik N Ghosh, Kenneth A Giuliano, R Terry Dunlay, Bruce Conway filed Critical Cellomics Inc
Priority to AU71319/00A priority Critical patent/AU7131900A/en
Publication of WO2000079241A2 publication Critical patent/WO2000079241A2/en
Publication of WO2000079241A3 publication Critical patent/WO2000079241A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G01N33/5079—Mitochondria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Abstract

The present invention provides methods, computer readable storage medium, and kits for identifying compounds that induce or inhibit macromolecule trafficking through the endosomal system, comprising treating cells that possess a luminescently-tagged macromolecule with a test compound, obtaining luminescent signals from the cells, converting the luminescent signals into digital data, and utilizing the digital data to determine whether the test compound has induced or inhibited macromolecule trafficking through the endosomal system.
PCT/US2000/040260 1999-06-21 2000-06-21 A system for cell-based screening WO2000079241A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU71319/00A AU7131900A (en) 1999-06-21 2000-06-21 A system for cell-based screening

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US14014399P 1999-06-21 1999-06-21
US60/140,143 1999-06-21
US09/352,171 US6759206B1 (en) 1997-02-27 1999-07-12 System for cell-based screening
US09/352,171 1999-07-12
US14836099P 1999-08-11 1999-08-11
US60/148,360 1999-08-11
US17031399P 1999-12-13 1999-12-13
US60/170,313 1999-12-13

Publications (2)

Publication Number Publication Date
WO2000079241A2 WO2000079241A2 (en) 2000-12-28
WO2000079241A3 true WO2000079241A3 (en) 2002-05-10

Family

ID=27495414

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/040260 WO2000079241A2 (en) 1999-06-21 2000-06-21 A system for cell-based screening

Country Status (2)

Country Link
AU (1) AU7131900A (en)
WO (1) WO2000079241A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
AU2073801A (en) 1999-12-09 2001-06-18 Cellomics, Inc. A system for cell-based screening
US7193706B2 (en) 2000-08-02 2007-03-20 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Computer interfaced scanning fluorescence lifetime microscope applied to directed evolution
AU2001296885A1 (en) * 2000-09-27 2002-04-08 Hyperion, Inc. Quantitative digital fluorography and related products and methods
WO2002041064A1 (en) 2000-11-17 2002-05-23 Universal Imaging Corporation Rapidly changing dichroic beamsplitter
WO2003014381A1 (en) 2001-08-10 2003-02-20 Ahram Biosystems Inc. System for detecting protease
US7295316B2 (en) * 2004-01-14 2007-11-13 Applera Corporation Fluorescent detector with automatic changing filters
EP1802975B1 (en) * 2004-10-13 2012-05-16 Evotec Technologies GmbH Method for identifying compounds that affect a transport of a protein through a membrane trafficking pathway
WO2007136724A2 (en) 2006-05-17 2007-11-29 Cellumen, Inc. Method for automated tissue analysis
CA3212002A1 (en) 2011-03-17 2012-09-20 Cernostics, Inc. Systems and compositions for diagnosing barrett's esophagus and methods of using the same
EP3163287B1 (en) * 2015-10-30 2022-03-23 Sysmex Corporation Cell information obtaining method and cell information obtaining apparatus
CN108918564B (en) * 2018-08-09 2021-02-02 陕西延长石油(集团)有限责任公司研究院 Analysis method suitable for shale mineral component quantification

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO1998045704A2 (en) * 1997-04-07 1998-10-15 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response
WO2000003246A2 (en) * 1998-07-13 2000-01-20 Cellomics, Inc. A system for cell-based screening
WO2000017643A2 (en) * 1998-09-18 2000-03-30 Cellomics, Inc. A system for cell-based screening
WO2000026408A2 (en) * 1998-10-30 2000-05-11 Cellomics, Inc. A system for cell-based screening
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening
WO2000070342A2 (en) * 1999-05-14 2000-11-23 Cellomics, Inc. Optical system analysis of cells

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO1998045704A2 (en) * 1997-04-07 1998-10-15 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response
WO2000003246A2 (en) * 1998-07-13 2000-01-20 Cellomics, Inc. A system for cell-based screening
WO2000017643A2 (en) * 1998-09-18 2000-03-30 Cellomics, Inc. A system for cell-based screening
WO2000026408A2 (en) * 1998-10-30 2000-05-11 Cellomics, Inc. A system for cell-based screening
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening
WO2000070342A2 (en) * 1999-05-14 2000-11-23 Cellomics, Inc. Optical system analysis of cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GIULIANO K A ET AL: "Fluorescent-protein biosensors: new tools for drug discovery", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 16, no. 3, 1 March 1998 (1998-03-01), pages 135 - 140, XP004108592, ISSN: 0167-7799 *

Also Published As

Publication number Publication date
WO2000079241A2 (en) 2000-12-28
AU7131900A (en) 2001-01-09

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