WO2000077251A3 - Forensic dna profiling method comprising immunological separation step - Google Patents

Forensic dna profiling method comprising immunological separation step Download PDF

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Publication number
WO2000077251A3
WO2000077251A3 PCT/GB2000/002203 GB0002203W WO0077251A3 WO 2000077251 A3 WO2000077251 A3 WO 2000077251A3 GB 0002203 W GB0002203 W GB 0002203W WO 0077251 A3 WO0077251 A3 WO 0077251A3
Authority
WO
WIPO (PCT)
Prior art keywords
profiling method
dna profiling
separation step
spermatozoa
forensic dna
Prior art date
Application number
PCT/GB2000/002203
Other languages
French (fr)
Other versions
WO2000077251A2 (en
Inventor
Matthew James Greenhalgh
Original Assignee
Astrazeneca Uk Ltd
Matthew James Greenhalgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astrazeneca Uk Ltd, Matthew James Greenhalgh filed Critical Astrazeneca Uk Ltd
Priority to AU52356/00A priority Critical patent/AU5235600A/en
Publication of WO2000077251A2 publication Critical patent/WO2000077251A2/en
Publication of WO2000077251A3 publication Critical patent/WO2000077251A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/02—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates involving antibodies to sugar part of glycoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction

Abstract

A DNA profiling method which comprises (i) contacting a sample believed to comprise spermatozoa and/or epithelial cells with antibodies specific for antigens presented on the spermatozoa and/or epithelial cells which antigens are not readily degraded, so as to form antibody-spermatozoa and/or antibody-epithelial cell complexes, (ii) separating the complex(es) from other material in the sample, (iii) isolating DNA from the complex(es) and (iv) determining the specific characteristics of the isolated DNA.
PCT/GB2000/002203 1999-06-11 2000-06-07 Forensic dna profiling method comprising immunological separation step WO2000077251A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU52356/00A AU5235600A (en) 1999-06-11 2000-06-07 Assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9913556.8 1999-06-11
GBGB9913556.8A GB9913556D0 (en) 1999-06-11 1999-06-11 Assays

Publications (2)

Publication Number Publication Date
WO2000077251A2 WO2000077251A2 (en) 2000-12-21
WO2000077251A3 true WO2000077251A3 (en) 2001-05-25

Family

ID=10855131

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002203 WO2000077251A2 (en) 1999-06-11 2000-06-07 Forensic dna profiling method comprising immunological separation step

Country Status (3)

Country Link
AU (1) AU5235600A (en)
GB (1) GB9913556D0 (en)
WO (1) WO2000077251A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7320891B2 (en) 2004-09-10 2008-01-22 Promega Corporation Methods and kits for isolating sperm cells
US8993292B2 (en) 2007-01-16 2015-03-31 Applied Biosystems Llc Methods and systems for differential extraction
EP2955521A1 (en) 2014-06-11 2015-12-16 Centre Hospitalier Universitaire Vaudois (CHUV) Methods for separating cells

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0186271A1 (en) * 1984-11-12 1986-07-02 THE LISTER INSTITUTE OF PREVENTIVE MEDICINE Royal National Orthopaedic Hospital Method of characterising a test sample of genomic DNA
EP0238329A2 (en) * 1986-03-19 1987-09-23 Zeneca Limited Improvements in genetic probes
US4741998A (en) * 1985-06-05 1988-05-03 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5: a new probe for sexual assault analyses
US5047508A (en) * 1985-06-05 1991-09-10 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5; a new probe for sexual assault analysis
EP0530009A2 (en) * 1991-08-27 1993-03-03 Zeneca Limited Method of characterising genomic DNA
US5436157A (en) * 1989-03-03 1995-07-25 The University Of Virginia Alumni Patents Foundation Human intra-acrosomal sperm antigen
US5605803A (en) * 1989-03-03 1997-02-25 University Of Virginia Alumni Patents Foundation Human sperm diagnostic
WO1998028622A1 (en) * 1996-12-20 1998-07-02 Fodstad Oeystein Method for characterization of abnormal cells
US5830472A (en) * 1996-06-28 1998-11-03 The University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and applications therefor
WO1999042129A1 (en) * 1998-02-23 1999-08-26 University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and application therefor

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0186271A1 (en) * 1984-11-12 1986-07-02 THE LISTER INSTITUTE OF PREVENTIVE MEDICINE Royal National Orthopaedic Hospital Method of characterising a test sample of genomic DNA
US4741998A (en) * 1985-06-05 1988-05-03 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5: a new probe for sexual assault analyses
US5047508A (en) * 1985-06-05 1991-09-10 The University Of Virginia Alumni Patents Foundation Monoclonal antibody to MHS-5; a new probe for sexual assault analysis
EP0238329A2 (en) * 1986-03-19 1987-09-23 Zeneca Limited Improvements in genetic probes
US5436157A (en) * 1989-03-03 1995-07-25 The University Of Virginia Alumni Patents Foundation Human intra-acrosomal sperm antigen
US5605803A (en) * 1989-03-03 1997-02-25 University Of Virginia Alumni Patents Foundation Human sperm diagnostic
EP0530009A2 (en) * 1991-08-27 1993-03-03 Zeneca Limited Method of characterising genomic DNA
US5830472A (en) * 1996-06-28 1998-11-03 The University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and applications therefor
WO1998028622A1 (en) * 1996-12-20 1998-07-02 Fodstad Oeystein Method for characterization of abnormal cells
WO1999042129A1 (en) * 1998-02-23 1999-08-26 University Of Virginia Patent Foundation Purified sperm surface antigen, monoclonal antibody therefor and application therefor

Also Published As

Publication number Publication date
WO2000077251A2 (en) 2000-12-21
AU5235600A (en) 2001-01-02
GB9913556D0 (en) 1999-08-11

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