WO2000068671A3 - Multiplexed fluorescent detection in microfluidic devices - Google Patents

Multiplexed fluorescent detection in microfluidic devices Download PDF

Info

Publication number
WO2000068671A3
WO2000068671A3 PCT/US2000/013217 US0013217W WO0068671A3 WO 2000068671 A3 WO2000068671 A3 WO 2000068671A3 US 0013217 W US0013217 W US 0013217W WO 0068671 A3 WO0068671 A3 WO 0068671A3
Authority
WO
WIPO (PCT)
Prior art keywords
excitation light
channel
microfluidic devices
fluorescent detection
orientation device
Prior art date
Application number
PCT/US2000/013217
Other languages
French (fr)
Other versions
WO2000068671A2 (en
Inventor
Kevin Maher
Timothy Smith
Torleif Bjornson
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to CA002373537A priority Critical patent/CA2373537A1/en
Priority to JP2000616405A priority patent/JP3815969B2/en
Priority to EP00930713A priority patent/EP1192447A2/en
Publication of WO2000068671A2 publication Critical patent/WO2000068671A2/en
Publication of WO2000068671A3 publication Critical patent/WO2000068671A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6478—Special lenses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/101—Scanning measuring head

Abstract

An optical detection and orientation device is provided comprising housing having an excitation light source. An optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focusing lens for focusing the emitted light onto the entry of an optical fiber, which serves as a confocal aperture, and means for accurately moving said housing over a small area in relation to a channel in a microfluidic device. The optical detection and orientation device finds use in identifying the center of the channel and detecting fluorophores in the channel during operations involving fluorescent signals.
PCT/US2000/013217 1999-05-12 2000-05-12 Multiplexed fluorescent detection in microfluidic devices WO2000068671A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002373537A CA2373537A1 (en) 1999-05-12 2000-05-12 Multiplexed fluorescent detection in microfluidic devices
JP2000616405A JP3815969B2 (en) 1999-05-12 2000-05-12 Multiplex fluorescence detection in microfluidic devices
EP00930713A EP1192447A2 (en) 1999-05-12 2000-05-12 Multiplexed fluorescent detection in microfluidic devices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13372799P 1999-05-12 1999-05-12
US60/133,727 1999-05-12

Publications (2)

Publication Number Publication Date
WO2000068671A2 WO2000068671A2 (en) 2000-11-16
WO2000068671A3 true WO2000068671A3 (en) 2001-02-15

Family

ID=22460037

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/013217 WO2000068671A2 (en) 1999-05-12 2000-05-12 Multiplexed fluorescent detection in microfluidic devices

Country Status (5)

Country Link
US (2) US6399952B1 (en)
EP (1) EP1192447A2 (en)
JP (1) JP3815969B2 (en)
CA (1) CA2373537A1 (en)
WO (1) WO2000068671A2 (en)

Families Citing this family (215)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
DE10011529T1 (en) 1998-05-01 2011-09-01 Gen-Probe Incorporated Automatic diagnostic analyzer and method
NZ513390A (en) 1999-03-19 2003-01-31 Genencor Int Multi-through hole testing plate for high throughput screening
JP2000304698A (en) * 1999-04-16 2000-11-02 Canon Inc Method and device for fluorescence measurement, and substrate suited therefor
US6838680B2 (en) * 1999-05-12 2005-01-04 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
WO2001067674A2 (en) 2000-03-03 2001-09-13 Qualcomm Incorporated Method and apparatus for participating in group communication services in an existing communication system
US6563581B1 (en) 2000-07-14 2003-05-13 Applera Corporation Scanning system and method for scanning a plurality of samples
US20030160957A1 (en) * 2000-07-14 2003-08-28 Applera Corporation Scanning system and method for scanning a plurality of samples
US6531041B1 (en) * 2000-07-20 2003-03-11 Symyx Technologies, Inc. Multiplexed capillary electrophoresis system with rotatable photodetector
US7027683B2 (en) * 2000-08-15 2006-04-11 Nanostream, Inc. Optical devices with fluidic systems
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
EP1409712A4 (en) * 2001-04-10 2008-05-14 Bioprocessors Corp Microfermentor device and cell based screening method
AU2002316302A1 (en) * 2001-07-12 2003-01-29 Aclara Biosciences, Inc. Submersible light-directing member for material excitation in microfluidic devices
WO2003008902A1 (en) * 2001-07-17 2003-01-30 Caliper Technologies Corp. Methods and systems for alignment of detection optics
US7023007B2 (en) * 2001-07-17 2006-04-04 Caliper Life Sciences, Inc. Methods and systems for alignment of detection optics
US7376304B2 (en) * 2001-09-27 2008-05-20 Bio-Rad Laboratories, Inc. Biochemical assay detection using a fiber optic exciter
US7218810B2 (en) * 2001-09-27 2007-05-15 Bio-Rad Laboratories, Inc. Biochemical assay detection in a liquid receptacle using a fiber optic exciter
US9157860B2 (en) * 2002-05-16 2015-10-13 Applied Biosystems, Llc Achromatic lens array
US6982166B2 (en) * 2002-05-16 2006-01-03 Applera Corporation Lens assembly for biological testing
JP4106977B2 (en) * 2002-06-21 2008-06-25 株式会社日立製作所 Analysis chip and analyzer
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
WO2004040295A1 (en) * 2002-10-31 2004-05-13 Nanostream, Inc. Parallel detection chromatography systems
WO2004046709A1 (en) * 2002-11-20 2004-06-03 Richard Fritz Sauter Method for analyzing molecules for molecule sequencing and spectrometer therefor
US6987263B2 (en) * 2002-12-13 2006-01-17 Nanostream, Inc. High throughput systems and methods for parallel sample analysis
US20060076482A1 (en) * 2002-12-13 2006-04-13 Hobbs Steven E High throughput systems and methods for parallel sample analysis
US7087444B2 (en) 2002-12-16 2006-08-08 Palo Alto Research Center Incorporated Method for integration of microelectronic components with microfluidic devices
EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
US6970240B2 (en) 2003-03-10 2005-11-29 Applera Corporation Combination reader
US20060078893A1 (en) * 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
JP2005006553A (en) * 2003-06-19 2005-01-13 Olympus Corp Apparatus for cell culture detection
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US7570443B2 (en) * 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US7998435B2 (en) 2003-09-19 2011-08-16 Life Technologies Corporation High density plate filler
US9492820B2 (en) 2003-09-19 2016-11-15 Applied Biosystems, Llc High density plate filler
US8277760B2 (en) 2003-09-19 2012-10-02 Applied Biosystems, Llc High density plate filler
US20050226780A1 (en) * 2003-09-19 2005-10-13 Donald Sandell Manual seal applicator
US7695688B2 (en) * 2003-09-19 2010-04-13 Applied Biosystems, Llc High density plate filler
US20060013984A1 (en) * 2003-09-19 2006-01-19 Donald Sandell Film preparation for seal applicator
WO2005028629A2 (en) * 2003-09-19 2005-03-31 Applera Corporation Whole genome expression analysis system
US20050232818A1 (en) * 2003-09-19 2005-10-20 Donald Sandell Single sheet seal applicator and cartridge
US20060011305A1 (en) * 2003-09-19 2006-01-19 Donald Sandell Automated seal applicator
US7002688B2 (en) * 2003-10-16 2006-02-21 Pria Diagnostics, Inc. Multilens optical assembly for a diagnostic device
AU2005222618A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
EP1726940A1 (en) * 2004-03-17 2006-11-29 Olympus Corporation Light measurement apparatus and light measurement method
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
ES2553097T3 (en) 2004-05-03 2015-12-04 Handylab, Inc. Processing of samples containing polynucleotides
AU2005299590B2 (en) 2004-10-27 2010-09-16 Cepheid Closed-system multi-stage nucleic acid amplification reactions
DE102004056787A1 (en) * 2004-11-24 2006-06-01 Heinrich-Heine-Universität Düsseldorf Apparatus and method for measuring fluorescence in multiple reaction spaces
US8008066B2 (en) 2005-03-10 2011-08-30 Gen-Probe Incorporated System for performing multi-formatted assays
WO2007044856A1 (en) * 2005-10-11 2007-04-19 The Johns Hopkins University Device and method for high-throughput stimulation, immunostaining, and visualization of single cells
US8449837B2 (en) * 2005-10-11 2013-05-28 The Johns Hopkins University Microfluidic device for high-throughput cellular gradient and dose response studies
JP4738134B2 (en) * 2005-10-26 2011-08-03 株式会社東芝 Analysis equipment
CA2571904A1 (en) * 2006-02-15 2007-08-15 Fio Corporation System and method of detecting pathogens
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7778503B2 (en) * 2006-03-31 2010-08-17 Nokia Corporation Electronic device having optical data connection of movable housing parts
US8900828B2 (en) * 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
WO2008002562A2 (en) * 2006-06-26 2008-01-03 Applera Corporation Compressible transparent sealing for open microplates
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CN101669026B (en) 2006-12-14 2014-05-07 生命技术公司 Methods and apparatus for measuring analytes using large scale FET arrays
CA2580589C (en) * 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
KR101422467B1 (en) * 2007-02-08 2014-07-24 삼성전자주식회사 A system and a method for detecting fluorescence in microfluidic chip
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CA2682826C (en) 2007-04-02 2013-08-13 Fio Corporation System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
US8702976B2 (en) * 2007-04-18 2014-04-22 Ondavia, Inc. Hand-held microfluidic testing device
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
JP2010530912A (en) 2007-06-22 2010-09-16 フィオ コーポレイション Manufacturing system and method for polymer microbeads doped with quantum dots
JP5507454B2 (en) * 2007-07-09 2014-05-28 フィオ コーポレイション System and method for improved fluorescence detection of target molecules in a test sample
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
FR2919143B1 (en) * 2007-07-16 2009-10-23 Commissariat Energie Atomique METHOD AND DEVICE FOR SIGMA-DELTA ALGORITHM MOTION DETECTION WITH ADAPTIVE THRESHOLD
KR101832658B1 (en) * 2007-07-23 2018-02-26 피오 코포레이션 A method and system for collating, storing, analyzing and enabling access to collected and analyzed data associated with biological and environmental test subjects
JP5628037B2 (en) * 2007-10-12 2014-11-19 フィオ コーポレイション Flow focusing method and system for forming concentrated microbeads, and microbeads formed in the system
US9176049B2 (en) * 2007-10-30 2015-11-03 Arkray Inc Method of optical analysis using reference cell and base plate correction and apparatus thereof
CN101946010B (en) 2007-12-21 2014-08-20 哈佛大学 Systems and methods for nucleic acid sequencing
US9017946B2 (en) 2008-06-23 2015-04-28 Canon U.S. Life Sciences, Inc. Systems and methods for monitoring the amplification of DNA
BRPI0915514A2 (en) 2008-06-25 2016-01-26 Fio Corp biohazard warning infrastructure system and method, biohazard warning device and a method for alerting a user
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
EP2313339A1 (en) 2008-07-21 2011-04-27 Valorbec S.E.C. A microfluidic device and method for fabricating the microfluidic device
US20100032582A1 (en) * 2008-08-07 2010-02-11 General Electric Company Fluorescence detection system and method
RU2515209C2 (en) 2008-08-29 2014-05-10 Эф-Ай-Оу Корпорейшн Disposable portable diagnostic instrument and appropriate system and method to investigate biological and natural samples
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US11951474B2 (en) 2008-10-22 2024-04-09 Life Technologies Corporation Fluidics systems for sequential delivery of reagents
US8546128B2 (en) * 2008-10-22 2013-10-01 Life Technologies Corporation Fluidics system for sequential delivery of reagents
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010080134A1 (en) 2008-12-19 2010-07-15 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
CN104374932A (en) 2009-01-13 2015-02-25 Fio公司 A handheld diagnostic test device used with an electronic device and a test cartridge in a rapid diagnostic test
EP2391883B1 (en) 2009-01-30 2018-03-07 Micronics, Inc. Portable high gain fluorescence detection system
US8637301B2 (en) 2009-03-02 2014-01-28 The Johns Hopkins University Microfluidic solution for high-throughput, droplet-based single molecule analysis with low reagent consumption
AU2010315580B2 (en) 2009-10-27 2014-11-06 President And Fellows Of Harvard College Droplet creation techniques
US8398418B2 (en) 2010-01-07 2013-03-19 Life Technologies Corporation Electronic connector having a clamping member urging a flow cell toward an electrical circuitry with an electrically conductive membrane disposed in between
JP5791634B2 (en) 2010-01-29 2015-10-07 マイクロニクス， インコーポレイテッド Sample-response microfluidic cartridge
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
DE102010003036B4 (en) * 2010-03-18 2022-06-23 Carl Zeiss Microscopy Gmbh Illumination device and microscope with an illumination device
CN105424665B (en) * 2010-04-15 2019-03-26 洛伊克德克斯有限公司 For quickly determining the equipment, system and method for medical conditions
US8858782B2 (en) 2010-06-30 2014-10-14 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
CN106198656B (en) 2010-08-18 2018-12-11 生命科技股份有限公司 The immersion coating of micropore for electrochemical detection device
WO2012054641A2 (en) * 2010-10-19 2012-04-26 The Johns Hopkins University Hydrodynamic particle separation and detection systems and methods
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US10146906B2 (en) 2010-12-30 2018-12-04 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
CN103534575B (en) * 2011-02-04 2016-08-10 环球生物研究株式会社 Automatically reaction/light measurement device and method thereof
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
US8718948B2 (en) 2011-02-24 2014-05-06 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
BR112013026451B1 (en) 2011-04-15 2021-02-09 Becton, Dickinson And Company system and method to perform molecular diagnostic tests on several samples in parallel and simultaneously amplification in real time in plurality of amplification reaction chambers
EP2522981A1 (en) * 2011-05-09 2012-11-14 Universiteit Twente Compact 2D light detection system for on-chip analysis
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2761305B1 (en) 2011-09-30 2017-08-16 Becton, Dickinson and Company Unitized reagent strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
US9194840B2 (en) 2012-01-19 2015-11-24 Life Technologies Corporation Sensor arrays and methods for making same
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014097287A1 (en) 2012-12-17 2014-06-26 Leukodx, Ltd. Systems and methods for detecting a biological condition
US10610861B2 (en) 2012-12-17 2020-04-07 Accellix Ltd. Systems, compositions and methods for detecting a biological condition
US20140170678A1 (en) 2012-12-17 2014-06-19 Leukodx Ltd. Kits, compositions and methods for detecting a biological condition
EP2934751B1 (en) 2012-12-21 2019-05-29 Micronics, Inc. Low elasticity films for microfluidic use
CN104919035B (en) 2012-12-21 2017-08-11 精密公司 Portable fluorescence detecting system and micro- determination box
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
EP2972404B1 (en) 2013-03-15 2021-11-24 Abbott Laboratories Automated diagnostic analyzers having rear accessible track systems and related methods
JP6351703B2 (en) 2013-03-15 2018-07-04 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ Automatic diagnostic analyzer with vertically arranged carousel and related methods
US20140264472A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
EP2972402B1 (en) 2013-03-15 2023-12-20 Abbott Laboratories Diagnostic analyzers with pretreatment carousels and related methods
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
WO2014182847A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Device for preparation and analysis of nucleic acids
CA2911303C (en) 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN105683980B (en) 2013-10-04 2018-08-24 生命科技股份有限公司 The method and system of effect model stage by stage is established in using the sequencing for terminating chemical substance
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
EP4053292A1 (en) 2014-06-26 2022-09-07 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
ES2789000T3 (en) 2014-10-10 2020-10-23 Quantapore Inc Nanopore-based polynucleotide analysis with mutually inactivating fluorescent labels
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
WO2016069939A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CA2972969A1 (en) 2015-01-12 2016-07-21 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3245605B1 (en) 2015-01-13 2022-04-20 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
EP3349899A4 (en) * 2015-09-14 2019-03-27 Singulex, Inc. Single molecule detection on a chip
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
CN115369161A (en) 2015-12-04 2022-11-22 10X 基因组学有限公司 Methods and compositions for nucleic acid analysis
JP6735348B2 (en) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド Systems, methods and media for de novo assembly of whole genome sequence data
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10520436B2 (en) * 2016-11-29 2019-12-31 Caduceus Biotechnology Inc. Dynamic focusing confocal optical scanning system
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. Systems and methods for analyzing datasets
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
EP3803397A2 (en) * 2018-05-25 2021-04-14 Five Prime Therapeutics, Inc. Enhanced cytometry for tissue characterization and screening
JP7430701B2 (en) 2018-07-23 2024-02-13 ディーエヌエー スクリプト Large-scale parallel enzymatic synthesis of nucleic acid strands
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
EP3906317A1 (en) 2019-01-03 2021-11-10 DNA Script One pot synthesis of sets of oligonucleotides
KR20230142832A (en) 2021-01-08 2023-10-11 셀라노메, 인크. Devices and methods for analyzing biological samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0564178A1 (en) * 1992-04-01 1993-10-06 The Regents Of The University Of California Scanning confocal microscope
WO1996034278A1 (en) * 1995-04-26 1996-10-31 Beckman Instruments, Inc. A multicapillary fluorescent detection system
US5730850A (en) * 1993-04-23 1998-03-24 Hitachi, Ltd. Capillary array electrophoresis system
WO1998049543A1 (en) * 1997-05-01 1998-11-05 Nanogen, Inc. Scanning optical detection system

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT380572B (en) * 1982-12-28 1986-06-10 List Hans OPTICAL SENSOR
JPH03235037A (en) * 1990-02-09 1991-10-21 Canon Inc Particle analyzing apparatus
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5436718A (en) * 1993-07-30 1995-07-25 Biolumin Corporation Mutli-functional photometer with movable linkage for routing optical fibers
US5397709A (en) * 1993-08-27 1995-03-14 Becton Dickinson And Company System for detecting bacterial growth in a plurality of culture vials
US5459325A (en) * 1994-07-19 1995-10-17 Molecular Dynamics, Inc. High-speed fluorescence scanner
US5571410A (en) 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5483075A (en) * 1994-11-01 1996-01-09 Perkin-Elmer Corporation Rotary scanning apparatus
US5614726A (en) * 1995-03-23 1997-03-25 Beckman Instruments, Inc. Automated optical alignment system and method using Raman scattering of capillary tube contents
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
US5528050A (en) * 1995-07-24 1996-06-18 Molecular Dynamics, Inc. Compact scan head with multiple scanning modalities

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0564178A1 (en) * 1992-04-01 1993-10-06 The Regents Of The University Of California Scanning confocal microscope
US5730850A (en) * 1993-04-23 1998-03-24 Hitachi, Ltd. Capillary array electrophoresis system
WO1996034278A1 (en) * 1995-04-26 1996-10-31 Beckman Instruments, Inc. A multicapillary fluorescent detection system
WO1998049543A1 (en) * 1997-05-01 1998-11-05 Nanogen, Inc. Scanning optical detection system

Also Published As

Publication number Publication date
US20020179849A1 (en) 2002-12-05
JP2002544476A (en) 2002-12-24
WO2000068671A2 (en) 2000-11-16
CA2373537A1 (en) 2000-11-16
EP1192447A2 (en) 2002-04-03
JP3815969B2 (en) 2006-08-30
US6614030B2 (en) 2003-09-02
US6399952B1 (en) 2002-06-04

Similar Documents

Publication Publication Date Title
WO2000068671A3 (en) Multiplexed fluorescent detection in microfluidic devices
RU2182328C2 (en) Fluorescent microscope
WO2006115863A3 (en) Compact optical detection system for microfluidic devices
DE50007163D1 (en) Transmitted light illumination device for microscopes
CA2415376A1 (en) Scanning system and method for scanning a plurality of samples
EP1557708A3 (en) Microscope with evanescent illumination
JP2001166214A5 (en)
DE69634660D1 (en) OPTICAL FLUORESCENT SENSOR
DE59805578D1 (en) Fiber detector for the detection of scattered light or fluorescent light from a liquid suspension
AU2003221505A1 (en) Sample carrier comprising integrated optics
RU2000104231A (en) FLUORESCENT MICROSCOPE
PT973040E (en) DETECTOR AND DISCRIMINATION DEVICE OF IOES CHANNELS
WO2005037085A3 (en) Multilens optical assembly for a diagnostic device
WO2002074055A3 (en) Epifluorecence microscope with improved image contrast
EP1406082A4 (en) Fluorescence reader
DE59808993D1 (en) METHOD AND DEVICE FOR FLUORESCENCE CORRELATION SPECTROSCOPY, ESPECIALLY FOR MULTICOLOR FLUORESCENCE CORRELATION SPECTROSCOPY
CN108780216B (en) Imaging system and method using scattering to reduce autofluorescence and improve uniformity
WO2000072077A8 (en) Scanning device, especially for detecting fluorescent light
AU2002343069A1 (en) A dual wavelength optical fluorescence analyser
ATE119288T1 (en) ELECTROFORETIC CAPILLARY WITH BUILT-IN OPTICAL DEVICE.
BR0301700A (en) Single-mode fiber optic and optical communication system for multichannel signal transmission
WO2006009792A3 (en) Projection microscope
JPH058976B2 (en)
RU2000131574A (en) OPTICAL Biosensor of irreversible cholinesterase inhibitors in the air
JP2004012975A (en) Fluorescent microscope and vertical illuminator for fluorescent observation, and vertical illumination method

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 616405

Kind code of ref document: A

Format of ref document f/p: F

ENP Entry into the national phase

Ref document number: 2373537

Country of ref document: CA

Ref country code: CA

Ref document number: 2373537

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000930713

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000930713

Country of ref document: EP