WO2000068671A3 - Multiplexed fluorescent detection in microfluidic devices - Google Patents
Multiplexed fluorescent detection in microfluidic devices Download PDFInfo
- Publication number
- WO2000068671A3 WO2000068671A3 PCT/US2000/013217 US0013217W WO0068671A3 WO 2000068671 A3 WO2000068671 A3 WO 2000068671A3 US 0013217 W US0013217 W US 0013217W WO 0068671 A3 WO0068671 A3 WO 0068671A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- excitation light
- channel
- microfluidic devices
- fluorescent detection
- orientation device
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6478—Special lenses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/10—Scanning
- G01N2201/101—Scanning measuring head
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00930713A EP1192447A2 (en) | 1999-05-12 | 2000-05-12 | Multiplexed fluorescent detection in microfluidic devices |
JP2000616405A JP3815969B2 (en) | 1999-05-12 | 2000-05-12 | Multiplex fluorescence detection in microfluidic devices |
CA002373537A CA2373537A1 (en) | 1999-05-12 | 2000-05-12 | Multiplexed fluorescent detection in microfluidic devices |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13372799P | 1999-05-12 | 1999-05-12 | |
US60/133,727 | 1999-05-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000068671A2 WO2000068671A2 (en) | 2000-11-16 |
WO2000068671A3 true WO2000068671A3 (en) | 2001-02-15 |
Family
ID=22460037
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/013217 WO2000068671A2 (en) | 1999-05-12 | 2000-05-12 | Multiplexed fluorescent detection in microfluidic devices |
Country Status (5)
Country | Link |
---|---|
US (2) | US6399952B1 (en) |
EP (1) | EP1192447A2 (en) |
JP (1) | JP3815969B2 (en) |
CA (1) | CA2373537A1 (en) |
WO (1) | WO2000068671A2 (en) |
Families Citing this family (215)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6048734A (en) | 1995-09-15 | 2000-04-11 | The Regents Of The University Of Michigan | Thermal microvalves in a fluid flow method |
US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
US8337753B2 (en) | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
EP1614474B1 (en) | 1998-05-01 | 2007-08-15 | Gen-Probe Incorporated | Incubator for automatic analyser |
EP1165235B1 (en) * | 1999-03-19 | 2011-09-28 | Life Technologies Corporation | Method of screening mutated cells |
JP2000304698A (en) * | 1999-04-16 | 2000-11-02 | Canon Inc | Method and device for fluorescence measurement, and substrate suited therefor |
US6838680B2 (en) * | 1999-05-12 | 2005-01-04 | Aclara Biosciences, Inc. | Multiplexed fluorescent detection in microfluidic devices |
US20020151040A1 (en) | 2000-02-18 | 2002-10-17 | Matthew O' Keefe | Apparatus and methods for parallel processing of microvolume liquid reactions |
ES2389057T3 (en) | 2000-03-03 | 2012-10-22 | Qualcomm Incorporated | Procedure and apparatus for participating in group communication services in an existing communication system |
US20030160957A1 (en) * | 2000-07-14 | 2003-08-28 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
US6563581B1 (en) | 2000-07-14 | 2003-05-13 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
US6531041B1 (en) * | 2000-07-20 | 2003-03-11 | Symyx Technologies, Inc. | Multiplexed capillary electrophoresis system with rotatable photodetector |
US7027683B2 (en) * | 2000-08-15 | 2006-04-11 | Nanostream, Inc. | Optical devices with fluidic systems |
US6692700B2 (en) | 2001-02-14 | 2004-02-17 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
US7010391B2 (en) | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
US7323140B2 (en) | 2001-03-28 | 2008-01-29 | Handylab, Inc. | Moving microdroplets in a microfluidic device |
US6852287B2 (en) | 2001-09-12 | 2005-02-08 | Handylab, Inc. | Microfluidic devices having a reduced number of input and output connections |
US20030077817A1 (en) * | 2001-04-10 | 2003-04-24 | Zarur Andrey J. | Microfermentor device and cell based screening method |
AU2002316302A1 (en) * | 2001-07-12 | 2003-01-29 | Aclara Biosciences, Inc. | Submersible light-directing member for material excitation in microfluidic devices |
US7023007B2 (en) * | 2001-07-17 | 2006-04-04 | Caliper Life Sciences, Inc. | Methods and systems for alignment of detection optics |
WO2003008902A1 (en) * | 2001-07-17 | 2003-01-30 | Caliper Technologies Corp. | Methods and systems for alignment of detection optics |
US7376304B2 (en) * | 2001-09-27 | 2008-05-20 | Bio-Rad Laboratories, Inc. | Biochemical assay detection using a fiber optic exciter |
US7218810B2 (en) * | 2001-09-27 | 2007-05-15 | Bio-Rad Laboratories, Inc. | Biochemical assay detection in a liquid receptacle using a fiber optic exciter |
US9157860B2 (en) * | 2002-05-16 | 2015-10-13 | Applied Biosystems, Llc | Achromatic lens array |
US6982166B2 (en) | 2002-05-16 | 2006-01-03 | Applera Corporation | Lens assembly for biological testing |
JP4106977B2 (en) * | 2002-06-21 | 2008-06-25 | 株式会社日立製作所 | Analysis chip and analyzer |
US7452712B2 (en) * | 2002-07-30 | 2008-11-18 | Applied Biosystems Inc. | Sample block apparatus and method of maintaining a microcard on a sample block |
US8277753B2 (en) | 2002-08-23 | 2012-10-02 | Life Technologies Corporation | Microfluidic transfer pin |
WO2004040295A1 (en) * | 2002-10-31 | 2004-05-13 | Nanostream, Inc. | Parallel detection chromatography systems |
AU2002363822A1 (en) * | 2002-11-20 | 2004-06-15 | Richard Fritz Sauter | Method for analyzing molecules for molecule sequencing and spectrometer therefor |
US6987263B2 (en) * | 2002-12-13 | 2006-01-17 | Nanostream, Inc. | High throughput systems and methods for parallel sample analysis |
US20060076482A1 (en) * | 2002-12-13 | 2006-04-13 | Hobbs Steven E | High throughput systems and methods for parallel sample analysis |
US7087444B2 (en) | 2002-12-16 | 2006-08-08 | Palo Alto Research Center Incorporated | Method for integration of microelectronic components with microfluidic devices |
EP1608952B1 (en) | 2002-12-20 | 2016-08-10 | Life Technologies Corporation | Assay apparatus and method using microfluidic arrays |
US6970240B2 (en) | 2003-03-10 | 2005-11-29 | Applera Corporation | Combination reader |
US20060078893A1 (en) * | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
US7820030B2 (en) * | 2003-04-16 | 2010-10-26 | Handylab, Inc. | System and method for electrochemical detection of biological compounds |
US7148043B2 (en) * | 2003-05-08 | 2006-12-12 | Bio-Rad Laboratories, Inc. | Systems and methods for fluorescence detection with a movable detection module |
JP2005006553A (en) * | 2003-06-19 | 2005-01-13 | Olympus Corp | Apparatus for cell culture detection |
JP4996248B2 (en) | 2003-07-31 | 2012-08-08 | ハンディーラブ インコーポレイテッド | Processing of particle-containing samples |
US20060029948A1 (en) * | 2003-09-19 | 2006-02-09 | Gary Lim | Sealing cover and dye compatibility selection |
US20050232818A1 (en) * | 2003-09-19 | 2005-10-20 | Donald Sandell | Single sheet seal applicator and cartridge |
US20050226780A1 (en) * | 2003-09-19 | 2005-10-13 | Donald Sandell | Manual seal applicator |
US20060011305A1 (en) * | 2003-09-19 | 2006-01-19 | Donald Sandell | Automated seal applicator |
US7998435B2 (en) | 2003-09-19 | 2011-08-16 | Life Technologies Corporation | High density plate filler |
WO2005029041A2 (en) * | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
US8277760B2 (en) | 2003-09-19 | 2012-10-02 | Applied Biosystems, Llc | High density plate filler |
US9492820B2 (en) | 2003-09-19 | 2016-11-15 | Applied Biosystems, Llc | High density plate filler |
US7695688B2 (en) * | 2003-09-19 | 2010-04-13 | Applied Biosystems, Llc | High density plate filler |
US7570443B2 (en) | 2003-09-19 | 2009-08-04 | Applied Biosystems, Llc | Optical camera alignment |
US20060013984A1 (en) * | 2003-09-19 | 2006-01-19 | Donald Sandell | Film preparation for seal applicator |
US7002688B2 (en) * | 2003-10-16 | 2006-02-21 | Pria Diagnostics, Inc. | Multilens optical assembly for a diagnostic device |
CA2559171A1 (en) | 2004-03-12 | 2005-09-29 | Biotrove, Inc. | Nanoliter array loading |
EP1726940A1 (en) * | 2004-03-17 | 2006-11-29 | Olympus Corporation | Light measurement apparatus and light measurement method |
US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
AU2005241080B2 (en) | 2004-05-03 | 2011-08-11 | Handylab, Inc. | Processing polynucleotide-containing samples |
EP1805318B1 (en) | 2004-10-27 | 2014-09-03 | Cepheid | Closed-system multi-stage nucleic acid amplification reactions |
DE102004056787A1 (en) * | 2004-11-24 | 2006-06-01 | Heinrich-Heine-Universität Düsseldorf | Apparatus and method for measuring fluorescence in multiple reaction spaces |
US7794659B2 (en) | 2005-03-10 | 2010-09-14 | Gen-Probe Incorporated | Signal measuring system having a movable signal measuring device |
WO2007044888A2 (en) * | 2005-10-11 | 2007-04-19 | The Johns Hopkins Univerisity | Microfluidic device and method for high-throughput cellular gradient and dose response studies |
WO2007044856A1 (en) * | 2005-10-11 | 2007-04-19 | The Johns Hopkins University | Device and method for high-throughput stimulation, immunostaining, and visualization of single cells |
JP4738134B2 (en) * | 2005-10-26 | 2011-08-03 | 株式会社東芝 | Analysis equipment |
CA2571904A1 (en) * | 2006-02-15 | 2007-08-15 | Fio Corporation | System and method of detecting pathogens |
US8883490B2 (en) | 2006-03-24 | 2014-11-11 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
US7998708B2 (en) * | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
DK2001990T3 (en) | 2006-03-24 | 2016-10-03 | Handylab Inc | Integrated microfluidic sample processing system and method for its use |
WO2007113604A1 (en) * | 2006-03-31 | 2007-10-11 | Nokia Corporation | Electronic device having optical data connection of movable housing parts |
US8900828B2 (en) * | 2006-05-01 | 2014-12-02 | Cepheid | Methods and apparatus for sequential amplification reactions |
EP2047910B1 (en) | 2006-05-11 | 2012-01-11 | Raindance Technologies, Inc. | Microfluidic device and method |
US8119352B2 (en) * | 2006-06-20 | 2012-02-21 | Cepheld | Multi-stage amplification reactions by control of sequence replication times |
US20080006202A1 (en) * | 2006-06-26 | 2008-01-10 | Applera Corporation | Compressible transparent sealing for open microplates |
US8709787B2 (en) | 2006-11-14 | 2014-04-29 | Handylab, Inc. | Microfluidic cartridge and method of using same |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US11339430B2 (en) | 2007-07-10 | 2022-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
EP2677309B9 (en) | 2006-12-14 | 2014-11-19 | Life Technologies Corporation | Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range |
CA2580589C (en) * | 2006-12-19 | 2016-08-09 | Fio Corporation | Microfluidic detection system |
US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
KR101422467B1 (en) * | 2007-02-08 | 2014-07-24 | 삼성전자주식회사 | A system and a method for detecting fluorescence in microfluidic chip |
US9029085B2 (en) * | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
US8360321B2 (en) | 2007-04-02 | 2013-01-29 | Fio Corporation | System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology |
US8702976B2 (en) * | 2007-04-18 | 2014-04-22 | Ondavia, Inc. | Hand-held microfluidic testing device |
WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
JP2010530912A (en) | 2007-06-22 | 2010-09-16 | フィオ コーポレイション | Manufacturing system and method for polymer microbeads doped with quantum dots |
CN101809433A (en) * | 2007-07-09 | 2010-08-18 | Fio公司 | Systems and methods for enhancing fluorescent detection of target molecules in a test sample |
US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
USD621060S1 (en) | 2008-07-14 | 2010-08-03 | Handylab, Inc. | Microfluidic cartridge |
US20090136385A1 (en) | 2007-07-13 | 2009-05-28 | Handylab, Inc. | Reagent Tube |
US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US8324372B2 (en) | 2007-07-13 | 2012-12-04 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
US8133671B2 (en) | 2007-07-13 | 2012-03-13 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US9618139B2 (en) | 2007-07-13 | 2017-04-11 | Handylab, Inc. | Integrated heater and magnetic separator |
FR2919143B1 (en) * | 2007-07-16 | 2009-10-23 | Commissariat Energie Atomique | METHOD AND DEVICE FOR SIGMA-DELTA ALGORITHM MOTION DETECTION WITH ADAPTIVE THRESHOLD |
CA2761176C (en) * | 2007-07-23 | 2012-07-31 | Fio Corporation | A method and system for collating, storing, analyzing and enabling access to collected and analyzed data associated with biological and environmental test subjects |
EP2209549A4 (en) * | 2007-10-12 | 2014-03-05 | Fio Corp | Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto |
JP5442447B2 (en) * | 2007-10-30 | 2014-03-12 | アークレイ株式会社 | Sample analysis method and apparatus |
EP2235210B1 (en) | 2007-12-21 | 2015-03-25 | President and Fellows of Harvard College | Methods for nucleic acid sequencing |
US9017946B2 (en) * | 2008-06-23 | 2015-04-28 | Canon U.S. Life Sciences, Inc. | Systems and methods for monitoring the amplification of DNA |
BRPI0915514A2 (en) | 2008-06-25 | 2016-01-26 | Fio Corp | biohazard warning infrastructure system and method, biohazard warning device and a method for alerting a user |
USD618820S1 (en) | 2008-07-11 | 2010-06-29 | Handylab, Inc. | Reagent holder |
USD787087S1 (en) | 2008-07-14 | 2017-05-16 | Handylab, Inc. | Housing |
WO2010009365A1 (en) | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
WO2010009543A1 (en) * | 2008-07-21 | 2010-01-28 | Valorbec S.E.C. | A microfluidic device and method for fabricating the microfluidic device |
US20100032582A1 (en) * | 2008-08-07 | 2010-02-11 | General Electric Company | Fluorescence detection system and method |
BRPI0917839A2 (en) | 2008-08-29 | 2015-11-24 | Fio Corp | single-use portable diagnostic test device, and associated system and method for testing environmental and biological test samples |
US20110218123A1 (en) * | 2008-09-19 | 2011-09-08 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
US11951474B2 (en) | 2008-10-22 | 2024-04-09 | Life Technologies Corporation | Fluidics systems for sequential delivery of reagents |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US8546128B2 (en) | 2008-10-22 | 2013-10-01 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
EP3290531B1 (en) | 2008-12-19 | 2019-07-24 | President and Fellows of Harvard College | Particle-assisted nucleic acid sequencing |
US9805165B2 (en) | 2009-01-13 | 2017-10-31 | Fio Corporation | Handheld diagnostic test device and method for use with an electronic device and a test cartridge in a rapid diagnostic test |
WO2010088514A1 (en) | 2009-01-30 | 2010-08-05 | Micronics, Inc. | Portable high gain fluorescence detection system |
US8637301B2 (en) | 2009-03-02 | 2014-01-28 | The Johns Hopkins University | Microfluidic solution for high-throughput, droplet-based single molecule analysis with low reagent consumption |
EP3461558B1 (en) | 2009-10-27 | 2021-03-17 | President and Fellows of Harvard College | Droplet creation techniques |
US8398418B2 (en) | 2010-01-07 | 2013-03-19 | Life Technologies Corporation | Electronic connector having a clamping member urging a flow cell toward an electrical circuitry with an electrically conductive membrane disposed in between |
KR101851117B1 (en) | 2010-01-29 | 2018-04-23 | 마이크로닉스 인코포레이티드. | Sample-to-answer microfluidic cartridge |
US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
EP2534267B1 (en) | 2010-02-12 | 2018-04-11 | Raindance Technologies, Inc. | Digital analyte analysis |
DE102010003036B4 (en) * | 2010-03-18 | 2022-06-23 | Carl Zeiss Microscopy Gmbh | Illumination device and microscope with an illumination device |
EP3239717B1 (en) * | 2010-04-15 | 2020-05-27 | Accellix Ltd | System and method for determination of a medical condition |
CN103154718B (en) | 2010-06-30 | 2015-09-23 | 生命科技公司 | The electric charge accumulation circuit of sensing ion and method |
US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
CN103221810B (en) | 2010-08-18 | 2016-08-03 | 生命科技股份有限公司 | Immersion coating for the micropore of electrochemical detection device |
US9476819B2 (en) | 2010-10-19 | 2016-10-25 | The Johns Hopkins University | Hydrodynamic particle separation and detection systems and methods |
EP2659408B1 (en) | 2010-12-29 | 2019-03-27 | Life Technologies Corporation | Time-warped background signal for sequencing-by-synthesis operations |
US20130060482A1 (en) | 2010-12-30 | 2013-03-07 | Life Technologies Corporation | Methods, systems, and computer readable media for making base calls in nucleic acid sequencing |
EP2658999B1 (en) | 2010-12-30 | 2019-03-13 | Life Technologies Corporation | Models for analyzing data from sequencing-by-synthesis operations |
WO2012092515A2 (en) | 2010-12-30 | 2012-07-05 | Life Technologies Corporation | Methods, systems, and computer readable media for nucleic acid sequencing |
WO2012105712A1 (en) | 2011-02-04 | 2012-08-09 | ユニバーサル・バイオ・リサーチ株式会社 | Automatic response/light measurement device and method therefor |
EP2675819B1 (en) | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
EP2678664B1 (en) | 2011-02-24 | 2019-08-07 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
BR112013026451B1 (en) | 2011-04-15 | 2021-02-09 | Becton, Dickinson And Company | system and method to perform molecular diagnostic tests on several samples in parallel and simultaneously amplification in real time in plurality of amplification reaction chambers |
EP2522981A1 (en) * | 2011-05-09 | 2012-11-14 | Universiteit Twente | Compact 2D light detection system for on-chip analysis |
DE202012013668U1 (en) | 2011-06-02 | 2019-04-18 | Raindance Technologies, Inc. | enzyme quantification |
US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
USD692162S1 (en) | 2011-09-30 | 2013-10-22 | Becton, Dickinson And Company | Single piece reagent holder |
WO2013049706A1 (en) | 2011-09-30 | 2013-04-04 | Becton, Dickinson And Company | Unitized reagent strip |
WO2013067202A1 (en) | 2011-11-04 | 2013-05-10 | Handylab, Inc. | Polynucleotide sample preparation device |
US9194840B2 (en) | 2012-01-19 | 2015-11-24 | Life Technologies Corporation | Sensor arrays and methods for making same |
CN104204812B (en) | 2012-02-03 | 2018-01-05 | 贝克顿·迪金森公司 | The external file that compatibility determines between distributing and test for molecule diagnostic test |
US9646132B2 (en) | 2012-05-11 | 2017-05-09 | Life Technologies Corporation | Models for analyzing data from sequencing-by-synthesis operations |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
MX364957B (en) | 2012-08-14 | 2019-05-15 | 10X Genomics Inc | Microcapsule compositions and methods. |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US20150376609A1 (en) | 2014-06-26 | 2015-12-31 | 10X Genomics, Inc. | Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US20140170678A1 (en) | 2012-12-17 | 2014-06-19 | Leukodx Ltd. | Kits, compositions and methods for detecting a biological condition |
US10610861B2 (en) | 2012-12-17 | 2020-04-07 | Accellix Ltd. | Systems, compositions and methods for detecting a biological condition |
EP2932266A4 (en) | 2012-12-17 | 2016-11-30 | Leukodx Ltd | Systems and methods for determining a chemical state |
JP6498125B2 (en) | 2012-12-21 | 2019-04-10 | マイクロニクス, インコーポレイテッド | Fluid circuit and associated manufacturing method |
JP2016509206A (en) | 2012-12-21 | 2016-03-24 | マイクロニクス, インコーポレイテッド | Portable fluorescence detection system and microassay cartridge |
US10518262B2 (en) | 2012-12-21 | 2019-12-31 | Perkinelmer Health Sciences, Inc. | Low elasticity films for microfluidic use |
CA2900481A1 (en) | 2013-02-08 | 2014-08-14 | 10X Genomics, Inc. | Polynucleotide barcode generation |
US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
CN116794337A (en) | 2013-03-15 | 2023-09-22 | 雅培制药有限公司 | Automated diagnostic analyzer with vertically disposed carousel and related methods |
CN105264366B (en) | 2013-03-15 | 2019-04-16 | 生命科技公司 | Chemical sensor with consistent sensor surface area |
CN108196079B (en) | 2013-03-15 | 2021-10-08 | 雅培制药有限公司 | Diagnostic analyzer with pre-processing carousel and related methods |
CN105745546B (en) | 2013-03-15 | 2017-10-13 | 雅培制药有限公司 | With below can injection system automated diagnostic analyzer and correlation technique |
WO2014182844A1 (en) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
EP2994543B1 (en) | 2013-05-07 | 2018-08-15 | Micronics, Inc. | Device for preparation and analysis of nucleic acids |
WO2014182831A1 (en) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
EP3053072B1 (en) | 2013-10-04 | 2024-02-21 | Life Technologies Corporation | Methods and systems for modeling phasing effects in sequencing using termination chemistry |
EP3065712A4 (en) | 2013-11-08 | 2017-06-21 | President and Fellows of Harvard College | Microparticles, methods for their preparation and use |
US9476853B2 (en) | 2013-12-10 | 2016-10-25 | Life Technologies Corporation | System and method for forming microwells |
US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
US11390921B2 (en) | 2014-04-01 | 2022-07-19 | Adaptive Biotechnologies Corporation | Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs) |
US10066265B2 (en) | 2014-04-01 | 2018-09-04 | Adaptive Biotechnologies Corp. | Determining antigen-specific t-cells |
AU2015243445B2 (en) | 2014-04-10 | 2020-05-28 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
JP2017526046A (en) | 2014-06-26 | 2017-09-07 | 10エックス ゲノミクス,インコーポレイテッド | Nucleic acid sequence assembly process and system |
AU2015330841B2 (en) | 2014-10-10 | 2019-08-15 | Quantapore, Inc. | Nanopore-based polymer analysis with mutually-quenching fluorescent labels |
US10676787B2 (en) | 2014-10-13 | 2020-06-09 | Life Technologies Corporation | Methods, systems, and computer-readable media for accelerated base calling |
US20160122817A1 (en) | 2014-10-29 | 2016-05-05 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequencing |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
KR20170106979A (en) | 2015-01-13 | 2017-09-22 | 10엑스 제노믹스, 인크. | System and method for visualizing structure variation and phase adjustment information |
US10854315B2 (en) | 2015-02-09 | 2020-12-01 | 10X Genomics, Inc. | Systems and methods for determining structural variation and phasing using variant call data |
EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
EP3262188B1 (en) | 2015-02-24 | 2021-05-05 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
US20180353957A1 (en) * | 2015-09-14 | 2018-12-13 | Singulex, Inc. | Single molecule detection on a chip |
CN108289797B (en) | 2015-10-13 | 2022-01-28 | 哈佛学院院长及董事 | Systems and methods for making and using gel microspheres |
SG11201804086VA (en) | 2015-12-04 | 2018-06-28 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
SG11201806757XA (en) | 2016-02-11 | 2018-09-27 | 10X Genomics Inc | Systems, methods, and media for de novo assembly of whole genome sequence data |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US10520436B2 (en) * | 2016-11-29 | 2019-12-31 | Caduceus Biotechnology Inc. | Dynamic focusing confocal optical scanning system |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
EP3625715A4 (en) | 2017-05-19 | 2021-03-17 | 10X Genomics, Inc. | Systems and methods for analyzing datasets |
US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
CN116064732A (en) | 2017-05-26 | 2023-05-05 | 10X基因组学有限公司 | Single cell analysis of transposase accessibility chromatin |
SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
SG11202009889VA (en) | 2018-04-06 | 2020-11-27 | 10X Genomics Inc | Systems and methods for quality control in single cell processing |
EP3803397A2 (en) * | 2018-05-25 | 2021-04-14 | Five Prime Therapeutics, Inc. | Enhanced cytometry for tissue characterization and screening |
US20210332351A1 (en) | 2018-07-23 | 2021-10-28 | Dna Script | Massively Parallel Enzymatic Synthesis of Nucleic Acid Strands |
EP3894593A2 (en) | 2018-12-13 | 2021-10-20 | DNA Script | Direct oligonucleotide synthesis on cells and biomolecules |
US20220356510A1 (en) | 2019-01-03 | 2022-11-10 | Dna Script | One Pot Synthesis of Sets of Oligonucleotides |
KR20230142832A (en) | 2021-01-08 | 2023-10-11 | 셀라노메, 인크. | Devices and methods for analyzing biological samples |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0564178A1 (en) * | 1992-04-01 | 1993-10-06 | The Regents Of The University Of California | Scanning confocal microscope |
WO1996034278A1 (en) * | 1995-04-26 | 1996-10-31 | Beckman Instruments, Inc. | A multicapillary fluorescent detection system |
US5730850A (en) * | 1993-04-23 | 1998-03-24 | Hitachi, Ltd. | Capillary array electrophoresis system |
WO1998049543A1 (en) * | 1997-05-01 | 1998-11-05 | Nanogen, Inc. | Scanning optical detection system |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AT380572B (en) * | 1982-12-28 | 1986-06-10 | List Hans | OPTICAL SENSOR |
JPH03235037A (en) * | 1990-02-09 | 1991-10-21 | Canon Inc | Particle analyzing apparatus |
US5750015A (en) | 1990-02-28 | 1998-05-12 | Soane Biosciences | Method and device for moving molecules by the application of a plurality of electrical fields |
US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
US5436718A (en) * | 1993-07-30 | 1995-07-25 | Biolumin Corporation | Mutli-functional photometer with movable linkage for routing optical fibers |
US5397709A (en) * | 1993-08-27 | 1995-03-14 | Becton Dickinson And Company | System for detecting bacterial growth in a plurality of culture vials |
US5459325A (en) * | 1994-07-19 | 1995-10-17 | Molecular Dynamics, Inc. | High-speed fluorescence scanner |
US5571410A (en) | 1994-10-19 | 1996-11-05 | Hewlett Packard Company | Fully integrated miniaturized planar liquid sample handling and analysis device |
US5483075A (en) * | 1994-11-01 | 1996-01-09 | Perkin-Elmer Corporation | Rotary scanning apparatus |
US5614726A (en) * | 1995-03-23 | 1997-03-25 | Beckman Instruments, Inc. | Automated optical alignment system and method using Raman scattering of capillary tube contents |
US5630924A (en) | 1995-04-20 | 1997-05-20 | Perseptive Biosystems, Inc. | Compositions, methods and apparatus for ultrafast electroseparation analysis |
US5528050A (en) * | 1995-07-24 | 1996-06-18 | Molecular Dynamics, Inc. | Compact scan head with multiple scanning modalities |
-
2000
- 2000-05-12 CA CA002373537A patent/CA2373537A1/en not_active Abandoned
- 2000-05-12 JP JP2000616405A patent/JP3815969B2/en not_active Expired - Lifetime
- 2000-05-12 US US09/569,963 patent/US6399952B1/en not_active Expired - Lifetime
- 2000-05-12 WO PCT/US2000/013217 patent/WO2000068671A2/en active Application Filing
- 2000-05-12 EP EP00930713A patent/EP1192447A2/en not_active Withdrawn
-
2002
- 2002-05-15 US US10/147,940 patent/US6614030B2/en not_active Expired - Lifetime
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0564178A1 (en) * | 1992-04-01 | 1993-10-06 | The Regents Of The University Of California | Scanning confocal microscope |
US5730850A (en) * | 1993-04-23 | 1998-03-24 | Hitachi, Ltd. | Capillary array electrophoresis system |
WO1996034278A1 (en) * | 1995-04-26 | 1996-10-31 | Beckman Instruments, Inc. | A multicapillary fluorescent detection system |
WO1998049543A1 (en) * | 1997-05-01 | 1998-11-05 | Nanogen, Inc. | Scanning optical detection system |
Also Published As
Publication number | Publication date |
---|---|
WO2000068671A2 (en) | 2000-11-16 |
JP2002544476A (en) | 2002-12-24 |
EP1192447A2 (en) | 2002-04-03 |
JP3815969B2 (en) | 2006-08-30 |
US6614030B2 (en) | 2003-09-02 |
US6399952B1 (en) | 2002-06-04 |
CA2373537A1 (en) | 2000-11-16 |
US20020179849A1 (en) | 2002-12-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2000068671A3 (en) | Multiplexed fluorescent detection in microfluidic devices | |
RU2182328C2 (en) | Fluorescent microscope | |
WO2006115863A3 (en) | Compact optical detection system for microfluidic devices | |
WO2002006796A3 (en) | Scanning system and method for scanning a plurality of samples | |
DE50007163D1 (en) | Transmitted light illumination device for microscopes | |
EP1557708A3 (en) | Microscope with evanescent illumination | |
JP2001166214A5 (en) | ||
ATE294380T1 (en) | OPTICAL FLUORESCENCE SENSOR | |
DE59805578D1 (en) | Fiber detector for the detection of scattered light or fluorescent light from a liquid suspension | |
AU2003221505A1 (en) | Sample carrier comprising integrated optics | |
WO2006028688A3 (en) | Method and apparatus for fiberscope | |
RU2000104231A (en) | FLUORESCENT MICROSCOPE | |
PT973040E (en) | DETECTOR AND DISCRIMINATION DEVICE OF IOES CHANNELS | |
EP1308789A3 (en) | Alignment apparatus, exposure apparatus using same, and method of manufacturing devices | |
WO2005037085A3 (en) | Multilens optical assembly for a diagnostic device | |
WO2002074055A3 (en) | Epifluorecence microscope with improved image contrast | |
EP1406082A4 (en) | Fluorescence reader | |
DE59808993D1 (en) | METHOD AND DEVICE FOR FLUORESCENCE CORRELATION SPECTROSCOPY, ESPECIALLY FOR MULTICOLOR FLUORESCENCE CORRELATION SPECTROSCOPY | |
CN108780216B (en) | Imaging system and method using scattering to reduce autofluorescence and improve uniformity | |
WO2000072077A8 (en) | Scanning device, especially for detecting fluorescent light | |
AU2002343069A1 (en) | A dual wavelength optical fluorescence analyser | |
DE69017283D1 (en) | Electroforetic capillary with built-in optical device. | |
BR0301700A (en) | Single-mode fiber optic and optical communication system for multichannel signal transmission | |
WO2006009792A3 (en) | Projection microscope | |
JPH058976B2 (en) |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): CA JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
AK | Designated states |
Kind code of ref document: A3 Designated state(s): CA JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A3 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
ENP | Entry into the national phase |
Ref country code: JP Ref document number: 2000 616405 Kind code of ref document: A Format of ref document f/p: F |
|
ENP | Entry into the national phase |
Ref document number: 2373537 Country of ref document: CA Ref country code: CA Ref document number: 2373537 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2000930713 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 2000930713 Country of ref document: EP |