WO2000060120A3 - Methods of detecting microorganism using immobilized probes - Google Patents

Methods of detecting microorganism using immobilized probes Download PDF

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Publication number
WO2000060120A3
WO2000060120A3 PCT/US2000/008773 US0008773W WO0060120A3 WO 2000060120 A3 WO2000060120 A3 WO 2000060120A3 US 0008773 W US0008773 W US 0008773W WO 0060120 A3 WO0060120 A3 WO 0060120A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
detecting
immobilized probes
detecting microorganism
microorganism
Prior art date
Application number
PCT/US2000/008773
Other languages
French (fr)
Other versions
WO2000060120A2 (en
WO2000060120A9 (en
Inventor
T Christian Boles
Original Assignee
Mosaic Technologies
T Christian Boles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies, T Christian Boles filed Critical Mosaic Technologies
Priority to EP00921605A priority Critical patent/EP1173613A2/en
Priority to CA002371732A priority patent/CA2371732A1/en
Priority to AU41898/00A priority patent/AU4189800A/en
Priority to JP2000609609A priority patent/JP2003503011A/en
Publication of WO2000060120A2 publication Critical patent/WO2000060120A2/en
Publication of WO2000060120A3 publication Critical patent/WO2000060120A3/en
Publication of WO2000060120A9 publication Critical patent/WO2000060120A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Methods of detecting the presence or absence of a microorganism comprising detecting the presence or absence of microbiological target molecules in a test sample employing electrophoresis comprising immobilized capture probes are described. Methods for detecting mutation sites within a putative mutant target molecule are also disclosed.
PCT/US2000/008773 1999-04-02 2000-03-31 Methods of detecting microorganism using immobilized probes WO2000060120A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00921605A EP1173613A2 (en) 1999-04-02 2000-03-31 Methods of detecting microorganism using immobilized probes
CA002371732A CA2371732A1 (en) 1999-04-02 2000-03-31 Methods of detecting microorganism using immobilized probes
AU41898/00A AU4189800A (en) 1999-04-02 2000-03-31 Methods of detecting microorganism using immobilized probes
JP2000609609A JP2003503011A (en) 1999-04-02 2000-03-31 Methods for detecting microorganisms using immobilized probes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28609199A 1999-04-02 1999-04-02
US09/286,091 1999-04-02

Publications (3)

Publication Number Publication Date
WO2000060120A2 WO2000060120A2 (en) 2000-10-12
WO2000060120A3 true WO2000060120A3 (en) 2001-09-13
WO2000060120A9 WO2000060120A9 (en) 2002-08-29

Family

ID=23097034

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/008773 WO2000060120A2 (en) 1999-04-02 2000-03-31 Methods of detecting microorganism using immobilized probes

Country Status (5)

Country Link
EP (1) EP1173613A2 (en)
JP (1) JP2003503011A (en)
AU (1) AU4189800A (en)
CA (1) CA2371732A1 (en)
WO (1) WO2000060120A2 (en)

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Publication number Priority date Publication date Assignee Title
DE69835516D1 (en) 1997-05-16 2006-09-21 Exact Sciences Corp ELECTROPHORETIC ANALYSIS OF MOLECULES WITH IMMOBILIZED PROBES
JP2002518026A (en) * 1998-06-19 2002-06-25 エムティー テクノロジー， インコーポレイテッド Detection of non-viral organisms using SRPRNA
JP2003284597A (en) * 2002-03-29 2003-10-07 Fujirebio Inc Method for measurement of target nucleic acid and kit for the same
CN1860241A (en) * 2003-07-30 2006-11-08 财团法人理工学振兴会 Partially double stranded nucleic acid molecule
EP1508622A1 (en) * 2003-08-22 2005-02-23 Siegfried Prof. Dr. Scherer Detection of the peptide synthase responsible for emetic toxin production in bacillus cereus
WO2005047881A2 (en) 2003-11-05 2005-05-26 Exact Sciences Corporation Repetitive reversed-field affinity electrophoresis and uses therefor
US10914698B2 (en) 2006-11-16 2021-02-09 General Electric Company Sensing method and system
US9538657B2 (en) 2012-06-29 2017-01-03 General Electric Company Resonant sensor and an associated sensing method
US9536122B2 (en) 2014-11-04 2017-01-03 General Electric Company Disposable multivariable sensing devices having radio frequency based sensors
US9589686B2 (en) 2006-11-16 2017-03-07 General Electric Company Apparatus for detecting contaminants in a liquid and a system for use thereof
US9658178B2 (en) 2012-09-28 2017-05-23 General Electric Company Sensor systems for measuring an interface level in a multi-phase fluid composition
US20100134286A1 (en) * 2008-12-01 2010-06-03 General Electric Company Radio frequency based sensors employing analyte recognition element
US9081006B2 (en) 2007-02-07 2015-07-14 Lyzer Diagnostics, Inc. Rapid homogeneous immunoassay using electrophoresis
EP2184997A4 (en) * 2007-07-31 2010-11-03 Univ New York Diagnostic and treatment methods for characterizing bacterial microbiota in skin conditions
WO2011137911A2 (en) * 2010-05-07 2011-11-10 Quantibact A/S Method for generating a double stranded nucleic acid with a single stranded overhang
US8542023B2 (en) 2010-11-09 2013-09-24 General Electric Company Highly selective chemical and biological sensors
EP4043025A1 (en) 2011-06-08 2022-08-17 Translate Bio, Inc. Lipid nanoparticle compositions and methods for mrna delivery
US10598650B2 (en) 2012-08-22 2020-03-24 General Electric Company System and method for measuring an operative condition of a machine
WO2014031749A1 (en) 2012-08-22 2014-02-27 General Electric Company Wireless system and method for measuring an operative condition of a machine
US10684268B2 (en) 2012-09-28 2020-06-16 Bl Technologies, Inc. Sensor systems for measuring an interface level in a multi-phase fluid composition
WO2014123430A1 (en) * 2013-02-05 2014-08-14 Victoria Link Limited Novel bio-sensor for the detection of small molecules
CN107794232B (en) * 2016-08-31 2022-03-08 广州金羿噬菌体生物技术有限公司 Streptococcus antagonizing growth of propionibacterium acnes and gram-positive bacteria and metabolites thereof
KR101862198B1 (en) * 2016-09-30 2018-05-30 (주)스파크바이오파마 A method for target protein identification using thermal stability shift-based fluorescence difference in two-dimensional gel electrophoresis
CN108374052A (en) * 2018-03-20 2018-08-07 徐州工程学院 Probe set sequences for biochip test staphylococcus aureus
US20220396824A1 (en) * 2019-09-24 2022-12-15 Genepath Diagnostics Inc. Composition and method for improving detection of biomolecules in biofluid samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (en) * 1989-07-13 1991-02-28 Hitachi Ltd Hybridization method, gene mutation detection using same and apparatus therefor
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
WO1998051823A1 (en) * 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
WO1999030145A1 (en) * 1997-12-05 1999-06-17 Mosaic Technologies Slotted electrophoresis gel composition and methods of use thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4053900A (en) * 1999-04-02 2000-10-23 Exact Sciences Corporation Electrophoretic analysis of target molecules using adapter molecules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (en) * 1989-07-13 1991-02-28 Hitachi Ltd Hybridization method, gene mutation detection using same and apparatus therefor
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
WO1998051823A1 (en) * 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
WO1999030145A1 (en) * 1997-12-05 1999-06-17 Mosaic Technologies Slotted electrophoresis gel composition and methods of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199115, Derwent World Patents Index; Class B04, AN 1991-105680, XP002075496 *
REHMAN F ET AL: "Immobilization of acrylamide-modified oligonucleotides by co-polymerization", NUCLEIC ACIDS RESEARCH, vol. 27, no. 2, 15 February 1999 (1999-02-15), pages 649 - 55, XP002155808 *
See also references of EP1173613A2 *

Also Published As

Publication number Publication date
WO2000060120A2 (en) 2000-10-12
AU4189800A (en) 2000-10-23
WO2000060120A9 (en) 2002-08-29
JP2003503011A (en) 2003-01-28
CA2371732A1 (en) 2000-10-12
EP1173613A2 (en) 2002-01-23

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