WO2000056903A3 - IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) - Google Patents

IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) Download PDF

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Publication number
WO2000056903A3
WO2000056903A3 PCT/US2000/007618 US0007618W WO0056903A3 WO 2000056903 A3 WO2000056903 A3 WO 2000056903A3 US 0007618 W US0007618 W US 0007618W WO 0056903 A3 WO0056903 A3 WO 0056903A3
Authority
WO
WIPO (PCT)
Prior art keywords
pichia
transformed
improved methods
producing proteins
recombinant
Prior art date
Application number
PCT/US2000/007618
Other languages
French (fr)
Other versions
WO2000056903A2 (en
Inventor
Bruce L Zamost
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to AU39100/00A priority Critical patent/AU3910000A/en
Publication of WO2000056903A2 publication Critical patent/WO2000056903A2/en
Publication of WO2000056903A3 publication Critical patent/WO2000056903A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

Methylotrophic yeasts are useful hosts for the production of commercially valuable recombinant proteins. However, the development of large-scale cultures of recombinant methylotrophic yeasts has been hindered by the formation of precipitation in culture media. A new soluble minimal medium overcomes this problem. Moreover, new feeding schemes provide cultures of high biomass, which produce biologically active recombinant protein.
PCT/US2000/007618 1999-03-22 2000-03-21 IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) WO2000056903A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU39100/00A AU3910000A (en) 1999-03-22 2000-03-21 Improved methods for producing proteins in transformed (pichia)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27426399A 1999-03-22 1999-03-22
US09/274,263 1999-03-22

Publications (2)

Publication Number Publication Date
WO2000056903A2 WO2000056903A2 (en) 2000-09-28
WO2000056903A3 true WO2000056903A3 (en) 2000-12-28

Family

ID=23047491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/007618 WO2000056903A2 (en) 1999-03-22 2000-03-21 IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)

Country Status (2)

Country Link
AU (1) AU3910000A (en)
WO (1) WO2000056903A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7103800A (en) * 1999-09-08 2001-04-10 Zymogenetics Inc. Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase promoter and terminator
US6348331B1 (en) 1999-09-08 2002-02-19 Zymogenetics, Inc. Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 promoter
US7118901B2 (en) 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
EP1460425A1 (en) 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
AT501955B1 (en) 2005-02-23 2007-08-15 Univ Graz Tech MUTED AOX1 PROMOTERS
BRPI0616054B8 (en) 2005-09-14 2021-05-25 Sanofi Aventis Deutschland process for the preparation of insulin, an insulin analogue or an insulin derivative, porcine trypsin, method for its production, DNA and vector
EP2106447B1 (en) 2007-01-26 2013-04-17 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
EP2565272A3 (en) * 2008-02-06 2013-07-03 Biocon Limited Fermentation medias and processes thereof
WO2011053545A1 (en) * 2009-10-30 2011-05-05 Merck Sharp & Dohme Corp. Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
US11214610B2 (en) 2010-12-01 2022-01-04 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
BR112013029902A2 (en) * 2011-05-20 2016-12-20 Alderbio Holdings Llc high purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
AU2016273912B2 (en) * 2011-05-20 2018-08-02 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
US10150968B2 (en) 2011-08-19 2018-12-11 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
DK3184642T3 (en) * 2015-12-22 2019-08-12 Bisy E U yeast
CN114686487B (en) * 2021-11-08 2023-12-22 泰州博莱得利生物科技有限公司 Efficient expression method of cat and dog interleukin 11 (IL-11) in pichia pastoris and application thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0510693A2 (en) * 1991-04-25 1992-10-28 Research Corporation Technologies, Inc. Expression of human serum albumin in pichia pastoris
WO1995021928A1 (en) * 1994-02-15 1995-08-17 Smithkline Beecham Biologicals (S.A.) Process for the production of a heterologous protein from a methylotrophic yeast
GB2292382A (en) * 1994-08-17 1996-02-21 Univ Rockefeller Obesity(OB) polypeptides
WO1998002565A1 (en) * 1996-07-17 1998-01-22 Zymogenetics, Inc. TRANSFORMATION OF $i(PICHIA METHANOLICA)
US5854039A (en) * 1996-07-17 1998-12-29 Zymogenetics, Inc. Transformation of pichia methanolica

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0510693A2 (en) * 1991-04-25 1992-10-28 Research Corporation Technologies, Inc. Expression of human serum albumin in pichia pastoris
WO1995021928A1 (en) * 1994-02-15 1995-08-17 Smithkline Beecham Biologicals (S.A.) Process for the production of a heterologous protein from a methylotrophic yeast
GB2292382A (en) * 1994-08-17 1996-02-21 Univ Rockefeller Obesity(OB) polypeptides
WO1998002565A1 (en) * 1996-07-17 1998-01-22 Zymogenetics, Inc. TRANSFORMATION OF $i(PICHIA METHANOLICA)
US5854039A (en) * 1996-07-17 1998-12-29 Zymogenetics, Inc. Transformation of pichia methanolica

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
C.K. RAYMOND: "Recombinant protein expression in Pichia methanolica", GENE EXPRESSION SYSTEMS: USING NATURE FOR THE ART OF EXPRESSION, 16 November 1998 (1998-11-16), J.M. Fernandez and J.P. Hoeffler (Eds).1999 Academic press, INC., pages 193 - 209, XP000912263 *
J M CREGG ET AL: "Recent Advances in the expression of foreign genes in Pichia pastoris", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, vol. 11, no. 11, August 1993 (1993-08-01), pages 905 - 910, XP002100307, ISSN: 0733-222X *
RAYMOND C K ET AL: "Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase", YEAST,GB,CHICHESTER, SUSSEX, vol. 14, no. 1, 1 January 1998 (1998-01-01), pages 11 - 23, XP002090804, ISSN: 0749-503X *
YIYING ZHANG ET AL: "POSITIONAL CLONING OF THE MOUSE OBESE GENE AND ITS HUMAN HOMOLOGUE", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 372, no. 6505, 1 December 1994 (1994-12-01), pages 425 - 432, XP000602062, ISSN: 0028-0836 *
ZAMOST B L ET AL: "Production of recombinant leptin using a new yeast expression system: Pichia methanolica.", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 217, no. 1-2, 1999, 217th National Meeting of the American Chemical Society;Anaheim, California, USA; March 21-25, 1999, pages BIOT 075, XP000915144, ISSN: 0065-7727 *

Also Published As

Publication number Publication date
AU3910000A (en) 2000-10-09
WO2000056903A2 (en) 2000-09-28

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