WO2000055626A1 - Optical assay device and method - Google Patents
Optical assay device and method Download PDFInfo
- Publication number
- WO2000055626A1 WO2000055626A1 PCT/US2000/005837 US0005837W WO0055626A1 WO 2000055626 A1 WO2000055626 A1 WO 2000055626A1 US 0005837 W US0005837 W US 0005837W WO 0055626 A1 WO0055626 A1 WO 0055626A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- optically active
- active test
- base
- assay device
- absorbent material
- Prior art date
Links
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- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
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- OCGWQDWYSQAFTO-UHFFFAOYSA-N tellanylidenelead Chemical compound [Pb]=[Te] OCGWQDWYSQAFTO-UHFFFAOYSA-N 0.000 description 1
- 229910052714 tellurium Inorganic materials 0.000 description 1
- PORWMNRCUJJQNO-UHFFFAOYSA-N tellurium atom Chemical compound [Te] PORWMNRCUJJQNO-UHFFFAOYSA-N 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- XOLBLPGZBRYERU-UHFFFAOYSA-N tin dioxide Chemical compound O=[Sn]=O XOLBLPGZBRYERU-UHFFFAOYSA-N 0.000 description 1
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- 239000010936 titanium Substances 0.000 description 1
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- 210000002700 urine Anatomy 0.000 description 1
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- DRDVZXDWVBGGMH-UHFFFAOYSA-N zinc;sulfide Chemical compound [S-2].[Zn+2] DRDVZXDWVBGGMH-UHFFFAOYSA-N 0.000 description 1
- 229910001928 zirconium oxide Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
Definitions
- optical assay devices require that the user apply a discrete volume of sample (approximately 25 - 30 ⁇ L, ) on the surface and that the incubation times be controlled by user intervention.
- Sample incubates on the surface in a static mode as the surface is solid and impermeable.
- the drying process also requires user interaction to bring the adsorbent material into contact with the solid optical test surface from above the test surface .
- the solid surface optical assays are extremely sensitive, an improvement in sensitivity can be gained by using all of the available sample (dependent on sample processing but generally greater than 200 ⁇ L) . In many testing sites, the requirement for user intervention in timing and drying the optical test device is inconvenient and not cost effective.
- a front portion 28 of the base 12 includes an incurved cut-out 66.
- the frame 20 also includes a recessed area 67 behind the incurved cut-out 66.
- the recessed area 67 includes a ramp 68 extending from a bottom surface 69.
- the recessed area 67 communicates with the outer well 34.
- the film materials that may be used for the base optical material include, but are not limited to, amorphous silicon, polycrystalline silicon, lead telluride, titanium, germanium, cobalt, gallium, tellurium, iron oxide, or chromium, or the like.
- amorphous silicon film having a thickness between 1000 and 5000 A is preferably used as the base optical material.
- the applied sample flows across and through the layers of the optical stack 18 when the optical stack 18 does not contact the absorbent material, unaffected by the absorbent material 16, but at a much lower rate compared to when the optical stack 18 contacts the absorbent material 16.
- Surface tension of the fluid in contact with the optical stack may also delay flow through the optical layer.
- the device of the present invention allows the sample incubation to occur over a period of time to improve capture efficiency and also minimizes the user intervention required to complete the assay.
- the device provides an increase in assay performance by allowing all available sample to flow across the optical member and through channels within the optical member. Because the contact time of sample with the test surface is controlled, the device is less sensitive to variable flow rates than other prior art devices. Also, in the device of the present invention, the signal generation is inherent in the composition and construction of the flow through support .
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002366307A CA2366307A1 (en) | 1999-03-18 | 2000-03-06 | Optical assay device and method |
JP2000605207A JP2002539452A (en) | 1999-03-18 | 2000-03-06 | Optical assay device and method |
EP00916114A EP1166115A4 (en) | 1999-03-18 | 2000-03-06 | Optical assay device and method |
AU37271/00A AU772685B2 (en) | 1999-03-18 | 2000-03-06 | Optical assay device and method |
HK02104875.0A HK1043831A1 (en) | 1999-03-18 | 2002-06-28 | Optical assay device and method |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/272,641 US6287783B1 (en) | 1999-03-18 | 1999-03-18 | Optical assay device and method |
US09/272,641 | 1999-03-18 |
Publications (1)
Publication Number | Publication Date |
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WO2000055626A1 true WO2000055626A1 (en) | 2000-09-21 |
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PCT/US2000/005837 WO2000055626A1 (en) | 1999-03-18 | 2000-03-06 | Optical assay device and method |
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US (2) | US6287783B1 (en) |
EP (1) | EP1166115A4 (en) |
JP (1) | JP2002539452A (en) |
KR (1) | KR100674525B1 (en) |
CN (1) | CN100403029C (en) |
AU (1) | AU772685B2 (en) |
CA (1) | CA2366307A1 (en) |
HK (1) | HK1043831A1 (en) |
TW (1) | TW468048B (en) |
WO (1) | WO2000055626A1 (en) |
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- 2000-03-06 EP EP00916114A patent/EP1166115A4/en not_active Withdrawn
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- 2000-03-06 CN CNB008050775A patent/CN100403029C/en not_active Expired - Fee Related
- 2000-03-06 KR KR1020017011848A patent/KR100674525B1/en not_active IP Right Cessation
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1419387A1 (en) * | 2001-08-20 | 2004-05-19 | Proteome Systems Intellectual Property Pty Ltd. | Diagnostic testing process and apparatus |
EP1419387A4 (en) * | 2001-08-20 | 2005-12-14 | Proteome Systems Intellectual | Diagnostic testing process and apparatus |
US7205159B2 (en) | 2001-08-20 | 2007-04-17 | Proteome Systems Intellectual Property Pty Ltd. | Diagnostic testing process and apparatus |
JP2005512090A (en) * | 2001-12-12 | 2005-04-28 | プロテオム システムズ インテレクチュアル プロパティ プロプライエタリー リミテッド | Diagnostic inspection method |
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GB2474306A (en) * | 2009-10-12 | 2011-04-13 | Bioproducts Ltd | Methods and device for detecting an analyte |
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Also Published As
Publication number | Publication date |
---|---|
US6287783B1 (en) | 2001-09-11 |
KR100674525B1 (en) | 2007-01-26 |
AU3727100A (en) | 2000-10-04 |
AU772685B2 (en) | 2004-05-06 |
CN100403029C (en) | 2008-07-16 |
KR20020021777A (en) | 2002-03-22 |
EP1166115A1 (en) | 2002-01-02 |
CA2366307A1 (en) | 2000-09-21 |
JP2002539452A (en) | 2002-11-19 |
EP1166115A4 (en) | 2006-09-20 |
US20020064888A1 (en) | 2002-05-30 |
CN1343311A (en) | 2002-04-03 |
HK1043831A1 (en) | 2002-09-27 |
TW468048B (en) | 2001-12-11 |
US6770447B2 (en) | 2004-08-03 |
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