WO2000053812A3 - Replica amplification of nucleic acid arrays - Google Patents

Replica amplification of nucleic acid arrays Download PDF

Info

Publication number
WO2000053812A3
WO2000053812A3 PCT/US2000/006390 US0006390W WO0053812A3 WO 2000053812 A3 WO2000053812 A3 WO 2000053812A3 US 0006390 W US0006390 W US 0006390W WO 0053812 A3 WO0053812 A3 WO 0053812A3
Authority
WO
WIPO (PCT)
Prior art keywords
arrays
methods
replicas
immobilized
nucleic acids
Prior art date
Application number
PCT/US2000/006390
Other languages
French (fr)
Other versions
WO2000053812A2 (en
WO2000053812A9 (en
Inventor
George M Church
Robi D Mitra
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Priority to CA002370535A priority Critical patent/CA2370535C/en
Priority to AU38761/00A priority patent/AU3876100A/en
Priority to JP2000603433A priority patent/JP2003526331A/en
Priority to EP00917853A priority patent/EP1235929A2/en
Publication of WO2000053812A2 publication Critical patent/WO2000053812A2/en
Publication of WO2000053812A9 publication Critical patent/WO2000053812A9/en
Publication of WO2000053812A3 publication Critical patent/WO2000053812A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00657—One-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.
PCT/US2000/006390 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays WO2000053812A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002370535A CA2370535C (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays
AU38761/00A AU3876100A (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays
JP2000603433A JP2003526331A (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid array
EP00917853A EP1235929A2 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/267,496 US6485944B1 (en) 1997-10-10 1999-03-12 Replica amplification of nucleic acid arrays
US09/267,496 1999-03-12

Publications (3)

Publication Number Publication Date
WO2000053812A2 WO2000053812A2 (en) 2000-09-14
WO2000053812A9 WO2000053812A9 (en) 2001-10-25
WO2000053812A3 true WO2000053812A3 (en) 2002-07-11

Family

ID=23019033

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/006390 WO2000053812A2 (en) 1999-03-12 2000-03-10 Replica amplification of nucleic acid arrays

Country Status (6)

Country Link
US (3) US6485944B1 (en)
EP (2) EP1235929A2 (en)
JP (1) JP2003526331A (en)
AU (1) AU3876100A (en)
CA (3) CA2411514A1 (en)
WO (1) WO2000053812A2 (en)

Families Citing this family (325)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6306578B1 (en) 1999-03-19 2001-10-23 Genencor International, Inc. Multi-through hole testing plate for high throughput screening
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US8137906B2 (en) * 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
GB2360282A (en) * 2000-03-17 2001-09-19 Bioinvent Int Ab Making and using micro-arrays of biological materials
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
AU2001217380A1 (en) * 2000-10-23 2002-05-21 Ingeny Holding Bv Method and apparatus for detecting a mutaiton in a nucleic acid fragment in a sample
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
WO2002068684A2 (en) * 2001-02-23 2002-09-06 Pyrosequencing Ab Allele-specific primer extension assay
ZA200210369B (en) 2001-03-09 2004-07-08 Nugen Technologies Inc Methods and compositions for amplification or RNA sequences.
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
WO2003006625A2 (en) 2001-07-13 2003-01-23 Ambergen, Inc. Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
ATE414767T1 (en) * 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh NUCLEIC ACID LINKERS AND THEIR USE IN GENE SYNTHESIS
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
DE60301622T2 (en) * 2002-04-04 2006-06-14 Biotage Ab Uppsala PRIMER EXTENSION BASED PROCESS USING NUCLEOTIDES MARKED OVER COLD LINKERS
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
ES2864086T3 (en) 2002-08-23 2021-10-13 Illumina Cambridge Ltd Labeled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
WO2004029294A1 (en) * 2002-09-27 2004-04-08 Biotage Ab New sequencing method for sequencing rna molecules
JP4329322B2 (en) * 2002-10-04 2009-09-09 ソニー株式会社 INTERACTIVE ACTION DETECTION METHOD, BIO ASSAY DEVICE, AND BIO ASSY
WO2004050839A2 (en) 2002-11-27 2004-06-17 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
AU2003298706A1 (en) 2002-12-04 2004-06-23 Applera Corporation Multiplex amplification of polynucleotides
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
DE602004018631D1 (en) 2003-04-24 2009-02-05 Afshin Ahmadian EN
US20050039420A1 (en) * 2003-07-31 2005-02-24 Albritton Charles Wade Fluid sensing in a drip tray
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005063787A2 (en) 2003-12-23 2005-07-14 Applera Corporation Propargyl substituted nucleoside compounds and methods
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
EP1557464B1 (en) * 2004-01-23 2010-09-29 Sloning BioTechnology GmbH De novo enzymatic production of nucleic acid molecules
EP2248911A1 (en) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
WO2005085475A1 (en) 2004-03-01 2005-09-15 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
CA2559171A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
GB2413796B (en) * 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
AU2005230936B2 (en) 2004-03-26 2010-08-05 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US7833709B2 (en) 2004-05-28 2010-11-16 Wafergen, Inc. Thermo-controllable chips for multiplex analyses
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
US20060073501A1 (en) * 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
JP2008513782A (en) * 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド Apparatus and method for molecular analysis
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
KR100766750B1 (en) * 2004-12-13 2007-10-17 주식회사 엘지생명과학 Method for fabricating a biochip
WO2006099579A2 (en) * 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
SG162795A1 (en) 2005-06-15 2010-07-29 Callida Genomics Inc Single molecule arrays for genetic and chemical analysis
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
WO2007053719A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
GB2446084B (en) 2005-10-31 2011-03-02 Univ Columbia Synthesis of four color 3-o-allyl modified photocleavable fluorescent nucleotides and related methods
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
WO2007064300A1 (en) * 2005-11-30 2007-06-07 Agency For Science, Technology And Research Electrochemical method of detecting an analyte
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
RU2394915C2 (en) * 2006-03-24 2010-07-20 Александр Борисович Четверин Non-contact methods of detecting molecular colonies, sets of reagents and device for realising said methods
EP2071927A2 (en) 2006-09-28 2009-06-24 Illumina, Inc. Compositions and methods for nucleotide sequencing
EP2548647B1 (en) * 2006-10-20 2018-08-15 CLONDIAG GmbH Assay devices and methods for the detection of analytes
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CA2672315A1 (en) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) * 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
WO2009011808A1 (en) * 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
EP2940029B1 (en) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP2725107B1 (en) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified ddNTPs and nucleic acid comprising inosine with reversible terminators
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
GB2470672B (en) * 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US9115352B2 (en) * 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
US8470164B2 (en) 2008-06-25 2013-06-25 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
US8772473B2 (en) 2009-03-30 2014-07-08 The Regents Of The University Of California Mostly natural DNA sequencing by synthesis
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US10385391B2 (en) 2009-09-22 2019-08-20 President And Fellows Of Harvard College Entangled mate sequencing
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
US20120245053A1 (en) * 2009-12-04 2012-09-27 Hitachi, Ltd. GENE EXPRESSION ANALYSIS METHOD USING TWO DIMENSIONAL cDNA LIBRARY
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
JP5952813B2 (en) 2010-06-30 2016-07-13 ライフ テクノロジーズ コーポレーション Method and apparatus for testing ISFET arrays
AU2011226767B1 (en) 2010-06-30 2011-11-10 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US8731847B2 (en) 2010-06-30 2014-05-20 Life Technologies Corporation Array configuration and readout scheme
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
EP2589065B1 (en) 2010-07-03 2015-08-19 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
WO2012036679A1 (en) 2010-09-15 2012-03-22 Life Technologies Corporation Methods and apparatus for measuring analytes
EP2619564B1 (en) 2010-09-24 2016-03-16 Life Technologies Corporation Matched pair transistor circuits
US10392726B2 (en) 2010-10-08 2019-08-27 President And Fellows Of Harvard College High-throughput immune sequencing
CA2814049C (en) 2010-10-08 2021-07-13 President And Fellows Of Harvard College High-throughput single cell barcoding
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
WO2013063382A2 (en) 2011-10-28 2013-05-02 Illumina, Inc. Microarray fabrication system and method
EP2776165A2 (en) 2011-11-07 2014-09-17 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US20130189679A1 (en) 2011-12-20 2013-07-25 The Regents Of The University Of Michigan Pseudogenes and uses thereof
US10150993B2 (en) 2011-12-22 2018-12-11 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
CA2863121A1 (en) 2011-12-30 2013-07-04 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
JP2015503923A (en) 2012-01-09 2015-02-05 オスロ ウニヴェルスィテーツスィーケフース ハーエフＯｓｌｏ Ｕｎｉｖｅｒｓｉｔｅｔｓｓｙｋｅｈｕｓ Ｈｆ Methods and biomarkers for the analysis of colorectal cancer
WO2013106737A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9506117B2 (en) 2012-02-21 2016-11-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
CA2866254A1 (en) 2012-03-06 2013-09-12 Oslo Universitetssykehus Hf Gene signatures associated with efficacy of postmastectomy radiotherapy in breast cancer
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
EP2834370B1 (en) 2012-04-03 2019-01-02 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
EP2844774B1 (en) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna sequencing
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
EP3438285B1 (en) 2012-05-02 2020-10-14 Ibis Biosciences, Inc. Dna sequencing
ES2683707T3 (en) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. DNA sequencing
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2895626B1 (en) 2012-09-12 2019-12-18 The Regents of The University of California Accurate genome sequencing of single cells by single-stranded amplification and sequencing
KR101595159B1 (en) * 2012-12-07 2016-02-18 서울대학교산학협력단 Isolation method of biochemical molecules on microarray
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
EP2954065B1 (en) 2013-02-08 2021-07-28 10X Genomics, Inc. Partitioning and processing of analytes and other species
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
CN105263918B (en) 2013-03-08 2018-05-18 伊鲁米纳剑桥有限公司 Rhodamine compound and its purposes as fluorescent marker
CN105164106B (en) 2013-03-08 2018-04-03 伊鲁米纳剑桥有限公司 Polymethine compound and its purposes as fluorescent marker
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
CN105378108A (en) 2013-03-13 2016-03-02 雅培分子公司 Systems and methods for isolating nucleic acids
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
EP2971154A4 (en) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nucleic acid control panels
US9701999B2 (en) 2013-03-14 2017-07-11 Abbott Molecular, Inc. Multiplex methylation-specific amplification systems and methods
EP3533884A1 (en) 2013-03-15 2019-09-04 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
CN105051525B (en) 2013-03-15 2019-07-26 生命科技公司 Chemical device with thin conducting element
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
CN105264366B (en) 2013-03-15 2019-04-16 生命科技公司 Chemical sensor with consistent sensor surface area
CN105358709B (en) 2013-03-15 2018-12-07 雅培分子公司 System and method for detecting genome copy numbers variation
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
DK2970356T3 (en) 2013-03-15 2018-08-27 Illumina Cambridge Ltd Modified nucleosides or nucleotides
CN105283758B (en) 2013-03-15 2018-06-05 生命科技公司 Chemical sensor with consistent sensor surface area
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
PL3017065T3 (en) 2013-07-01 2019-03-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
JP6300260B2 (en) * 2013-08-28 2018-03-28 国立大学法人埼玉大学 Method for producing replica microarray and original microarray containing target substance produced by the method
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
DK3572527T3 (en) 2013-10-17 2021-08-23 Illumina Inc METHODS AND PREPARATIONS FOR THE MANUFACTURE OF NUCLEIC ACID LIBRARIES
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
WO2015085274A1 (en) 2013-12-05 2015-06-11 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
EP3628747B1 (en) 2013-12-05 2022-10-05 Centrillion Technology Holdings Corporation Fabrication of patterned arrays
CN105940024B (en) 2013-12-05 2019-03-15 生捷科技控股公司 The surface of modification
AU2014364180B2 (en) 2013-12-09 2021-03-04 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
US9909181B2 (en) 2013-12-13 2018-03-06 Northwestern University Biomarkers for post-traumatic stress states
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
CN114089597A (en) 2013-12-19 2022-02-25 Illumina公司 Substrate comprising a nanopatterned surface and method for the production thereof
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US20150203907A1 (en) * 2014-01-17 2015-07-23 Florida State University Research Foundation Genome capture and sequencing to determine genome-wide copy number variation
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10760109B2 (en) 2014-06-06 2020-09-01 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
CN114214314A (en) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 Digital PCR barcoding
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
CN106575322B (en) 2014-06-26 2019-06-18 10X基因组学有限公司 The method and system of nucleic acid sequence assembly
BR112016030008A2 (en) 2014-06-27 2017-10-24 Abbott Lab method for detecting human pegivirus 2 infection in an individual, for detecting human pegivirus 2 nucleic acid and for detecting human pegivirus 2 in a sample, and, composition
MX2017001134A (en) 2014-07-24 2017-10-11 Abbott Molecular Inc Compositions and methods for the detection and analysis of mycobacterium tuberculosis.
EP3194643B1 (en) 2014-09-17 2020-02-19 Ibis Biosciences, Inc. Sequencing by synthesis using pulse read optics
KR20170073667A (en) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US11198855B2 (en) 2014-11-13 2021-12-14 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional “super” helicases
KR102593647B1 (en) 2014-12-18 2023-10-26 라이프 테크놀로지스 코포레이션 High data rate integrated circuit with transmitter configuration
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
EP3234575B1 (en) 2014-12-18 2023-01-25 Life Technologies Corporation Apparatus for measuring analytes using large scale fet arrays
CN112126675B (en) 2015-01-12 2022-09-09 10X基因组学有限公司 Method and system for preparing nucleic acid sequencing library and library prepared by using same
CN107209814B (en) 2015-01-13 2021-10-15 10X基因组学有限公司 System and method for visualizing structural variation and phase information
CA2975529A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
EP3822361A1 (en) 2015-02-20 2021-05-19 Takara Bio USA, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
EP3527672B1 (en) * 2015-06-09 2022-10-05 Centrillion Technology Holdings Corporation Oligonucleotide arrays for sequencing nucleic acids
US10526664B2 (en) 2015-07-14 2020-01-07 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
US10392613B2 (en) 2015-07-14 2019-08-27 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
US20180207920A1 (en) 2015-07-17 2018-07-26 Illumina, Inc. Polymer sheets for sequencing applications
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
CN108779138B (en) 2015-09-28 2022-06-17 哥伦比亚大学董事会 Design and synthesis of nucleotides based on novel disulfide linkers for use as reversible terminators for DNA sequencing by synthesis
CN108289797B (en) 2015-10-13 2022-01-28 哈佛学院院长及董事 Systems and methods for making and using gel microspheres
US11207393B2 (en) 2015-10-16 2021-12-28 President And Fellows Of Harvard College Regulatory T cell PD-1 modulation for regulating T cell effector immune responses
US10774370B2 (en) 2015-12-04 2020-09-15 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
CN108431223A (en) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 Multiple pearls under per drop resolution
WO2017139260A1 (en) 2016-02-08 2017-08-17 RGENE, Inc. Multiple ligase compositions, systems, and methods
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
EP3430154B1 (en) 2016-03-14 2020-11-11 Rgene, Inc. Hyper-thermostable lysine-mutant ssdna/rna ligases
US20210309965A1 (en) 2016-03-21 2021-10-07 Dana-Farber Cancer Institute, Inc. T-cell exhaustion state-specific gene expression regulators and uses thereof
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
KR102171865B1 (en) 2016-05-18 2020-10-29 일루미나, 인코포레이티드 Self-assembled patterning using patterned hydrophobic surfaces
US11118233B2 (en) 2016-05-18 2021-09-14 The University Of Chicago BTK mutation and ibrutinib resistance
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
CA3020629A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
FI3522713T3 (en) 2016-10-03 2023-01-13 Fluorescent detection of amines and hydrazines and assaying methods thereof
EP3555290B1 (en) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN109476674B (en) 2016-12-22 2021-12-10 伊鲁米纳剑桥有限公司 Coumarin compound and application thereof as fluorescent marker
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018165207A1 (en) 2017-03-06 2018-09-13 Singular Genomic Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
CN106987645A (en) * 2017-05-05 2017-07-28 武汉爱基百客生物科技有限公司 A kind of apparent omics technology
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. Systems and methods for analyzing datasets
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018227091A1 (en) 2017-06-08 2018-12-13 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
CN110770356A (en) 2017-06-20 2020-02-07 生物辐射实验室股份有限公司 MDA using bead oligonucleotides
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
WO2019035955A1 (en) * 2017-08-15 2019-02-21 President And Fellows Of Harvard College High-throughput system using a cell-free expression system and in situ sequencing
JP7260553B2 (en) 2017-10-06 2023-04-18 ザ・ユニバーシティ・オブ・シカゴ Screening of T lymphocytes against cancer-specific antigens
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
EP4180534A1 (en) 2017-11-02 2023-05-17 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
AU2018372906A1 (en) 2017-11-22 2020-06-11 The Regents Of The University Of Michigan Compositions and methods for treating cancer
CN111566226A (en) * 2017-12-28 2020-08-21 Ador诊断有限公司 Method for rapid detection of the presence of nucleic acid target molecules
US11701656B2 (en) 2018-01-02 2023-07-18 The Regents Of The University Of Michigan Multi-droplet capture
CN112041459A (en) 2018-01-29 2020-12-04 圣祖德儿童研究医院 Nucleic acid amplification method
EP4324962A2 (en) 2018-01-31 2024-02-21 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
EP3775269A1 (en) 2018-03-26 2021-02-17 Qiagen Sciences LLC Integrative dna and rna library preparations and uses thereof
CN112262218A (en) 2018-04-06 2021-01-22 10X基因组学有限公司 System and method for quality control in single cell processing
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC Methods and systems for sample processing or analysis
EP3841202B1 (en) 2018-08-20 2023-10-04 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2020086834A1 (en) 2018-10-25 2020-04-30 Singular Genomics Systems, Inc. Nucleotide analogues
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US20220081714A1 (en) 2019-01-04 2022-03-17 Northwestern University Storing temporal data into dna
US20220332752A2 (en) 2019-01-08 2022-10-20 Singular Genomics Systems, Inc. Nucleotide cleavable linkers and uses thereof
CA3224572A1 (en) 2019-02-19 2020-08-27 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
SG11202012555UA (en) 2019-03-01 2021-01-28 Illumina Cambridge Ltd Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
US20200277670A1 (en) 2019-03-01 2020-09-03 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and uses as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
JP2023510454A (en) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド Pigments and compositions containing cyclooctatetraene
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
EP3859012A1 (en) 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
CN115349128A (en) 2020-02-13 2022-11-15 齐默尔根公司 Metagenomic libraries and natural product discovery platform
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
WO2021237292A1 (en) 2020-05-27 2021-12-02 Genetic Technologies Limited Methods of assessing risk of developing a severe response to coronavirus infection
CA3177293A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
US20220033900A1 (en) 2020-07-28 2022-02-03 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
KR20240004502A (en) 2021-05-05 2024-01-11 일루미나 케임브리지 리미티드 Fluorescent dyes containing bis-boron fused heterocycles and their use in sequencing
EP4341434A1 (en) 2021-05-20 2024-03-27 Illumina, Inc. Compositions and methods for sequencing by synthesis
AU2022413575A1 (en) 2021-12-16 2024-01-18 Illumina, Inc. Methods for metal directed cleavage of surface-bound polynucleotides
CN117813399A (en) 2021-12-20 2024-04-02 因美纳有限公司 Periodate compositions and methods for chemically cleaving surface-bound polynucleotides
CA3223274A1 (en) 2021-12-20 2023-06-29 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
FR3130844A1 (en) 2021-12-22 2023-06-23 Bforcure COMPOSITION AND METHOD FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES
WO2023135485A1 (en) 2022-01-13 2023-07-20 Oslo Universitetssykehus Hf Prostate cancer markers and uses thereof
WO2023152568A2 (en) 2022-02-10 2023-08-17 Oslo Universitetssykehus Hf Compositions and methods for characterizing lung cancer
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
CA3223125A1 (en) 2022-03-29 2023-10-05 Illumina, Inc. Chromenoquinoline dyes and uses in sequencing
CA3223037A1 (en) 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
CA3222797A1 (en) 2022-03-31 2023-10-05 Ramesh NEELAKANDAN Nucleosides and nucleotides with 3' vinyl blocking group
WO2023232829A1 (en) 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing
US20230416279A1 (en) 2022-06-28 2023-12-28 Illumina Cambridge Limited Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024068889A2 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998029736A1 (en) * 1996-12-31 1998-07-09 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
EP0895082A2 (en) * 1997-08-01 1999-02-03 Canon Kabushiki Kaisha Method of spotting probe on solid support, probe array and method of manufacturing thereof, and method of detecting target substance and method of identifying structure of target substance using probe array

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591567A (en) 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
FI71768C (en) 1984-02-17 1987-02-09 Orion Yhtymae Oy Enhanced nucleic acid reagents and process for their preparation.
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4981783A (en) 1986-04-16 1991-01-01 Montefiore Medical Center Method for detecting pathological conditions
SE8801070D0 (en) 1988-03-23 1988-03-23 Pharmacia Ab METHOD FOR IMMOBILIZING A DNA SEQUENCE ON A SOLID SUPPORT
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5382511A (en) 1988-08-02 1995-01-17 Gene Tec Corporation Method for studying nucleic acids within immobilized specimens
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
CA2031659A1 (en) 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
US5506350A (en) 1990-09-20 1996-04-09 Bittner; Michael L. Production of chromosome region specific DNA sequences and transamination
RU1794088C (en) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Method of dna nucleotide sequence determination and a device for its realization
US5426180A (en) 1991-03-27 1995-06-20 Research Corporation Technologies, Inc. Methods of making single-stranded circular oligonucleotides
ATE165397T1 (en) 1991-06-27 1998-05-15 Genelabs Tech Inc SCREENING TEST FOR THE DETECTION OF DNA-BINDING MOLECULES
US5578444A (en) 1991-06-27 1996-11-26 Genelabs Technologies, Inc. Sequence-directed DNA-binding molecules compositions and methods
US5437976A (en) 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
CA2122450C (en) * 1991-11-01 2004-07-13 Charles Phillip Morris Solid phase amplification process
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
DE69334048D1 (en) * 1992-01-28 2006-08-31 Harvard College PROCESS FOR NUCLEIN ACID HIBRIDIZATION USING UNIVERSAL DNA BINDING PROTEINS
EP1382386A3 (en) 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
RU2048522C1 (en) 1992-10-14 1995-11-20 Институт белка РАН Method of nucleic acid copying, method of their expression and a medium for their realization
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5484702A (en) 1994-01-27 1996-01-16 Research Foundation Of The State University Of New York At Buffalo Method for preselecting recombinant clones containing a specific nucleic acid sequence and subsequent transformation with preselected clones
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
FR2726286B1 (en) * 1994-10-28 1997-01-17 Genset Sa SOLID PHASE NUCLEIC ACID AMPLIFICATION PROCESS AND REAGENT KIT USEFUL FOR CARRYING OUT SAID PROCESS
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5908926A (en) 1995-03-16 1999-06-01 Duke University 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group
US5753439A (en) 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
US5545531A (en) 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
GB9517914D0 (en) 1995-09-02 1995-11-01 Tepnel Medical Ltd Identification of bases in nucleic acid sequences
ATE269908T1 (en) 1997-04-01 2004-07-15 Manteia S A METHOD FOR SEQUENCING NUCLEIC ACIDS
EP3034626A1 (en) 1997-04-01 2016-06-22 Illumina Cambridge Limited Method of nucleic acid sequencing
AU3199699A (en) * 1998-03-23 1999-10-18 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
WO1998029736A1 (en) * 1996-12-31 1998-07-09 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
EP0895082A2 (en) * 1997-08-01 1999-02-03 Canon Kabushiki Kaisha Method of spotting probe on solid support, probe array and method of manufacturing thereof, and method of detecting target substance and method of identifying structure of target substance using probe array

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DUGGAN D J ET AL: "EXPRESSION PROFILING USING CDNA MICROARRAYS", NATURE GENETICS, NEW YORK, NY, US, vol. 21, no. SUPPL, January 1999 (1999-01-01), pages 10 - 14, XP000865980, ISSN: 1061-4036 *
SCHENA M ET AL: "QUANTITATIVE MONITORING OF GENE EXPRESSION PATTERNS WITH A COMPLEMENTARY DNA MICROARRAY", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 270, no. 5235, 20 October 1995 (1995-10-20), pages 467 - 470, XP000644675, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
AU3876100A (en) 2000-09-28
CA2370535A1 (en) 2000-09-14
US6485944B1 (en) 2002-11-26
JP2003526331A (en) 2003-09-09
EP1291354A2 (en) 2003-03-12
CA2370535C (en) 2008-05-13
EP1291354A3 (en) 2003-12-10
EP1235929A2 (en) 2002-09-04
CA2411514A1 (en) 2000-09-14
CA2582809A1 (en) 2000-09-14
US20020120127A1 (en) 2002-08-29
WO2000053812A2 (en) 2000-09-14
US20020120126A1 (en) 2002-08-29
WO2000053812A9 (en) 2001-10-25

Similar Documents

Publication Publication Date Title
WO2000053812A3 (en) Replica amplification of nucleic acid arrays
WO2004067759A3 (en) Replication of nucleic acid arrays
EP1343874A4 (en) 3-hydroxypropionic acid and other organic compounds
PT1309861E (en) FUNCTIONAL ARRAYS OF PROTEINS
DE69933369D1 (en) Adressierbare protein arrays
WO2004061119A3 (en) Rolling circle amplification of rna
WO2002053582A3 (en) Process for producing peptides by using in vitrotranscription/translation system
CA2205353A1 (en) Continuous amplification reaction
WO2002077264A3 (en) Arrayed transfection method and uses related thereto
WO2001059432A3 (en) Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
WO2003000941A3 (en) Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
EP2267139A3 (en) Methods and means for obtaining modified phenotypes
DK1836302T3 (en) Ligation-based RNA amplification
CA2020958A1 (en) Nucleic acid sequence amplification methods
WO2005074467A3 (en) Matrix array nanobiosensor
CY1107918T1 (en) DIAGNOSIS LAMINS
WO2002020838A3 (en) Method and system for detecting nucleic acids
JP2001521765A5 (en)
WO1992020796A3 (en) D-type cyclin and uses related thereto
WO2001031055A3 (en) Method and kit for the screening and/or the quantification of multiple homologous nucleic acid sequences on arrays
EP0938097A3 (en) Memory reading circuit and SRAM
EP1264894A4 (en) Atp regeneration reaction systems and method of examining adenine nucleotide, method of detecting rna and method of amplifying atp by using the
FR2563533B1 (en) METHOD FOR AMPLIFYING THE EXPRESSION OF A DETERMINED GENE IN SUBACTIVE BACILLUS AND STRAINS OBTAINED
WO2002016649A3 (en) Probes and decoder oligonucleotides
WO2003062387A3 (en) Thermotoga neapolitana xylose isomerase polypeptides and nucleic acids encoding same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2000 603433

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2000917853

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 38761/00

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2370535

Country of ref document: CA

Kind code of ref document: A

AK Designated states

Kind code of ref document: C2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

COP Corrected version of pamphlet

Free format text: PAGES 1/12-12/12, DRAWINGS, REPLACED BY NEW PAGES 1/10-10/10; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

WWP Wipo information: published in national office

Ref document number: 2000917853

Country of ref document: EP

DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)