WO2000050891A1 - Bandelette d'analyse dotee d'une cellule pour fluide et procede d'analyse d'un echantillon - Google Patents

Bandelette d'analyse dotee d'une cellule pour fluide et procede d'analyse d'un echantillon Download PDF

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Publication number
WO2000050891A1
WO2000050891A1 PCT/US2000/005038 US0005038W WO0050891A1 WO 2000050891 A1 WO2000050891 A1 WO 2000050891A1 US 0005038 W US0005038 W US 0005038W WO 0050891 A1 WO0050891 A1 WO 0050891A1
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WO
WIPO (PCT)
Prior art keywords
cell
sample
fluid
antibody
driving fluid
Prior art date
Application number
PCT/US2000/005038
Other languages
English (en)
Inventor
John C. Mcnierney
William S. Gibbons, Jr.
Original Assignee
Fertility Acoustics Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fertility Acoustics Inc. filed Critical Fertility Acoustics Inc.
Priority to AU35047/00A priority Critical patent/AU3504700A/en
Priority to EP00913639A priority patent/EP1163513A1/fr
Priority to KR1020017010957A priority patent/KR20010108273A/ko
Publication of WO2000050891A1 publication Critical patent/WO2000050891A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4905—Determining clotting time of blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Definitions

  • the present invention relates generally to devices and methods for detecting and determining the amount of an analyte in a biological sample. More specifically, the invention relates to a device and a method for determining blood levels of hormones including, but not limited to, luteinizing hormone (LH) , estradiol, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH) , and/or progesterone. Additionally, the invention relates to detection and determination of endocrine dysfunctions in humans and other mammals.
  • hormones including, but not limited to, luteinizing hormone (LH) , estradiol, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH) , and/or progesterone.
  • LH luteinizing hormone
  • FSH follicle-stimulating hormone
  • TSH thyroid-stimulating hormone
  • progesterone progesterone
  • Physiological changes in humans and other mammals are often accompanied by changes in concentration of various blood components.
  • ovulation in human and other mammalian females is preceded by a surge in the plasma concentration of LH in the blood.
  • Commonly available tests used to detect this surge are urine based. Urine tests have several drawbacks. They are awkward and often messy, and, more importantly, they are not as accurate as blood tests.
  • the hormone In order for a detectable concentration of LH to be accumulated in the urine, the hormone must be released by the pituitary, circulate in the blood, be sequestered in the kidneys, and finally excreted. The completion of these processes can take as long as 12 hours after the actual plasma surge for sufficient amount of LH to accumulate in the urine.
  • LH surge detection is a blood test. Theoretically, the LH level can be known nearly instantaneously, maximizing a female's reproductive window. Currently, however, the results of a clinical blood test are not available for 12-24 hours, and the high cost (typically - $90) prevents wide spread use.
  • LH is not the only analyte of interest. It is also desirable to detect basal plasm estradiol and FSH levels. Basal plasma estradiol and FSH levels are used by fertility clinics to determine the potential for in vi tro fertilization (IVF) success. Basal estradiol levels are taken on the third day of the menstrual cycle, when the concentration should be at its lowest. Studies show that where day-three estradiol was greater than 75 pg/mL, there were no successful IVF pregnancies.
  • estradiol was greater than 45 pg/Ml and FSH was greater than 17 IU/L, there were also no successful IVF pregnancies.
  • basal estradiol and FSH are low (less than 46 pg/Ml and 18 IU/L, respectively)
  • IVF can be successful 33.8% of the time.
  • basal FSH and estradiol levels obtained simultaneously on day-three of the menstrual cycle are essential tests for determining ovarian reserve in infertile patients.
  • the term "ovarian reserve" reflects the future capacity of the ovaries to produce viable eggs.
  • the primary reason that FSH levels would be elevated is that the follicles are not maturing in response to hormonal stimulation by the pituitary. As a result, the pituitary secretes more FSH. Failure to respond reflects an absence of viable ova in the ovaries, and carries with it a poor prognosis for future pregnancies.
  • the cause for a particular patient's infertility can be diagnosed by monitoring various hormones. For example, elevated basal FSH indicates exhaustion of the ovaries, and offers a poor prognosis. In other cases, however, the cause for infertility is unrelated to the functioning of the reproductive system itself. For example, a disruption in TSH levels can cause an otherwise healthy reproductive system to become dysfunctional. In cases where the problem can be pinpointed to a secondary source, such as thyroid dysfunction, treatment can be highly successful. A less expensive method to screen patients for thyroid function will allow physicians to screen more patients more frequently.
  • luteal phase defects Another area where a cheaper and faster means of detecting and determining the levels of various hormones is in the detection of luteal phase defects, which affect 1-3% of infertile couples, and 1/3 of women with spontaneous abortion.
  • the luteal phase is the time in a normal menstrual cycle after the ovum has ruptured, but preceding menses. Insufficient production of estradiol, progesterone, and/or LH during this time will prevent the endometrium and/or ovum from developing adequately, making implantation impossible. If a physician determines the ovaries respond well enough (i.e. there are viable eggs left) , then other endocrine problems, such as luteal phase defects, can be controlled via appropriate medications. A more cost-effective means of screening patients for endocrine problems will, therefore, allow more pregnancies to be saved.
  • hormones in blood including, but not limited to, LH, estradiol, FSH, TSH, and/or progesterone
  • physiological changes include, but are not limited to, determination of ovarian state and proper function of the reproductive system, as well as detection of endocrine causality of infertility in human and other mammalian females.
  • the device of the present invention serves to determine the blood level of various hormones, such as, for example, estradiol, FSH, TSH, and/or progesterone, of a patient, and for providing the results to a user.
  • the device of the present invention may be operated with very little skill, and requires a minimum number of actions by the user to obtain a dependable analytical result.
  • a device according to the present invention has a fluid cell with a driving fluid therein, and a breakable barrier for selectively containing the driving fluid in the fluid cell.
  • the device also includes a sample cell having a collection chamber capable of receiving a sample to be analyzed, capable of receiving the driving fluid from the first cell, and capable of allowing the sample to mix with the driving fluid to provide a mixed sample.
  • the device has a filter capable of capturing particulate matter contained in the sample, and a fluid collection reservoir.
  • the device contains one or more types of antibodies capable of attaching to an analyte of interest.
  • a method begins by providing a device, such as that described above, and providing a sample to the collection cell. Next, the barrier is broken to provide the driving fluid to the collection cell, where the sample and the driving fluid are mixed. Then a first type of antibody is introduced to the sample.
  • the first type of antibody has an affinity for the analyte of interest to form a tagged analyte.
  • the method also includes filtering to remove particulate matter in the sample, and detecting the tagged analyte in the detection cell. The method may also collect the driving fluid in the collection reservoir .
  • Figure 1A is a perspective view of a device according to the present invention.
  • Figure IB is a cross-sectional view of the device shown in Figure 1;
  • Figures 2 and 3 are perspective views of devices according to the present invention.
  • Figures 4A and 4B show steps of a method according to the present invention.
  • the device 10 includes a fluid cell 13 having driving fluid 16 therein and a breakable barrier 19 for selectively containing the driving fluid 16 in the fluid cell 13.
  • the driving fluid 16 may include water, a buffer and blocking agents.
  • Suitable buffers include phosphate, tris, glycine and the like, generally in the molarity of 0.1 to 3.0.
  • Suitable blocking agents include bovine serum albumin, diluted serum, non-fat dry milk, and casein.
  • Other additives in the driving fluid may include heparin or an anti- microbial substance.
  • the breakable barrier 19 may be made from Mylar with a metalized coating including aluminum. Alternatively, the breakable barrier 19 may be made from a polymer with a fluorine treated gas barrier. The breakable barrier 19 is preferably located at a first edge 22 of the fluid cell 13 and may be broken by increasing the pressure in the fluid cell 13, for example by squeezing the fluid cell 13 between a thumb and index finger. When the breakable barrier 19 is broken, the driving fluid 16 flows into another cell, such as the sample cell 25.
  • the sample cell 25 has a collection chamber 28 capable of receiving a sample to be analyzed.
  • the sample cell 25 may have an orifice 31 through which the sample, such as a drop of blood, may be deposited.
  • the sample cell 25 is capable of receiving the driving fluid 16 upon breaking the breakable barrier 19, and is capable of allowing the sample to mix with the driving fluid 16 to provide a mixed sample.
  • the device shown in Figures 1A and IB also has a filter 33 capable of capturing particulate matter from the mixed sample.
  • the filter 33 may include glass fibers and may be contained in a filter cell 36. As the mixed sample moves through the filter 33, particulate matter in the mixed sample is captured in the filter 33 to provide a filtered mixed sample to another cell, such as the first antibody cell 39.
  • the first antibody cell 39 has a first type of antibody capable of attaching to an analyte of interest in the filtered mixed sample. If the analyte of interest is in the filtered mixed sample, the first type of antibody combines with the analyte to form a tagged analyte in the filtered mixed sample.
  • the first type of antibody may be provided in the first antibody cell 39 via a porous nonreactive carrier matrix. Such matrices are commonly used for nucleic acid and protein binding.
  • the matrix may include nitrocellulose or nylon.
  • the carrier matrix is nitrocellulose, the first type of antibody can be directly immobilized on the carrier matrix without the need of a chemical treatment. However, for other matrices, immobilization can be accomplished by well known techniques, such as treatment with cyanogen bromide or carbonyldiimidazole .
  • the first antibody may include a monoclonal antibody, a polyclonal antibody, or fragments thereof.
  • the particular choice for the first type of antibody will depend upon the analyte to be detected. For example for the detection of LH, specific antibodies to LH can be immobilized on the carrier matrix.
  • the first type of antibody may include a chromophore, fluorophore in order to make detection easier.
  • the detecting cell 42 may have a second type of antibody capable of attaching to the tagged analyte, if present in the filtered mixed sample, to provide a secondary tag on the tagged analyte (the "secondarily tagged analyte").
  • the second type of antibody may be capable of attaching to a second analyte of interest. In that situation, the second type of antibody attaches to the second analyte to provide a labeled analyte.
  • the second type of antibody is preferably immobilized within the detecting cell to capture and retain the analyte of interest.
  • the second type of antibody may be a monoclonal antibody, a polyclonal antibody, and/or fragments thereof.
  • the second type of antibody preferably includes a detectable molecule or complex, such as a chromophore and fluorescent molecules and complexes.
  • the detecting cell 42 provides the tagged analyte, the secondarily tagged analyte, and/or the labeled analyte, as the case may be, for detection and analysis.
  • the detecting cell 42 preferably allows the filtered mixed sample to flow out of the detecting cell 42.
  • the device may include a collection cell 45 having a fluid collection reservoir 48 for receiving fluid from the detecting cell 42.
  • the collection reservoir 48 may be an empty chamber, or alternatively, may have an absorbent material 49 therein for capturing fluid.
  • the detecting cell 42 preferably includes one or more translucent sides 50 for permitting the tagged analyte, the secondarily tagged analyte and/or the labeled analyte to be detected, as further described below.
  • Figure 2 depicts an alternative embodiment of the present invention, in which the filter cell 36 is placed between the fluid cell 13 and the sample cell 25.
  • Figure 3 depicts another embodiment of the present invention in which the first antibody cell 39 has been eliminated. Instead of providing a separate cell for housing the first type of antibody, the first type of antibody is included in the driving fluid 16.
  • the first type of antibody may be included in the filter 33.
  • Fluorescent labeled antibodies having an affinity for specific analytes are available commercially or can be prepared by using techniques known in the art. For example, kits for fluorescent tagging and labeling of antibodies are available commercially as molecular probes or from Pierce.
  • FIGS. 4A and 4B show steps of a method according to the present invention.
  • the method begins by providing a device (step 100) similar to that described above.
  • a sample such as human blood, is provided (step 103) to the collection cell.
  • the barrier is broken to release the driving fluid (step 106) in the fluid cell.
  • the barrier may be broken by increasing the pressure inside the fluid cell, for example by squeezing the fluid cell between the index finger and thumb of a person.
  • the sample is mixed (step 109) with the driving fluid to provide a mixed sample, and a first type of antibody is provided (step 112) to the sample. If the analyte of interest is present in the sample, the first type of antibody will tag the analyte.
  • the filter is used (step 115) to remove particulate matter introduced by the sample, and the device is analyzed (step 118) for the presence of tagged analytes.
  • a second type of antibody may be provided (step 121) to the mixed sample.
  • the second type of antibody may or may not have an affinity for the same analyte of interest as the first type of antibody.
  • the method may also include collecting (step 124) driving fluid in the collection reservoir. It will be recognized that the intensity of the color, fluorescence, or luminescence of the tagged analyte, the secondarily tagged analyte and/or the labeled analyte, as the case may be, is indicative of the amount of the chromophore, fluorophore, luminescent or other label. Therefore measuring such intensity is indicative of the amount of the analyte (s) of interest in the sample.
  • the color intensity may be measured by comparison to a color chart.
  • the color intensity may be measured by focusing a light 51 on the detecting cell 42, and receiving transmitted light with a detector 54, or by using the apparatuses described in U.S. Patent application serial number 09/208,648, which is hereby incorporated by reference.

Abstract

L'invention se rapporte à un dispositif comportant une cellule pour fluide (13) contenant un fluide d'entraînement, et une barrière susceptible d'être rompue (22) qui permet de retenir sélectivement le fluide d'entraînement dans ladite cellule. Ledit dispositif comporte également une cellule pour échantillon (25) comportant une chambre de collecte (28) conçue pour recevoir un échantillon à analyser, susceptible de recevoir le fluide d'entraînement provenant de la première cellule et conçue pour permettre le mélange de l'échantillon et du fluide d'entraînement de manière à délivrer un échantillon mélangé. Ce dispositif comporte en outre un filtre (36) conçu pour retenir les matières particulaires contenues dans l'échantillon, ainsi qu'un réservoir de collecte de fluide (45). Ledit dispositif contient un ou plusieurs types d'anticorps susceptibles de se fixer à un analyte d'intérêt. L'invention se rapporte également à un procédé d'utilisation dudit dispositif pour détecter un analyte.
PCT/US2000/005038 1999-02-26 2000-02-25 Bandelette d'analyse dotee d'une cellule pour fluide et procede d'analyse d'un echantillon WO2000050891A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU35047/00A AU3504700A (en) 1999-02-26 2000-02-25 Analyzing strip having a fluid cell and a method of analyzing a sample
EP00913639A EP1163513A1 (fr) 1999-02-26 2000-02-25 Bandelette d'analyse dotee d'une cellule pour fluide et procede d'analyse d'un echantillon
KR1020017010957A KR20010108273A (ko) 1999-02-26 2000-02-25 유체셀을 갖는 분석 스트립 및 샘플 분석방법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12214099P 1999-02-26 1999-02-26
US60/122,140 1999-02-26

Publications (1)

Publication Number Publication Date
WO2000050891A1 true WO2000050891A1 (fr) 2000-08-31

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Application Number Title Priority Date Filing Date
PCT/US2000/005038 WO2000050891A1 (fr) 1999-02-26 2000-02-25 Bandelette d'analyse dotee d'une cellule pour fluide et procede d'analyse d'un echantillon

Country Status (9)

Country Link
EP (1) EP1163513A1 (fr)
KR (1) KR20010108273A (fr)
AR (1) AR022779A1 (fr)
AU (1) AU3504700A (fr)
CO (1) CO5280182A1 (fr)
GT (1) GT200000033A (fr)
RU (1) RU2001126123A (fr)
SV (1) SV2002000032A (fr)
WO (1) WO2000050891A1 (fr)

Cited By (23)

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EP1033575A2 (fr) * 1999-03-01 2000-09-06 Bayer Corporation Dispositif pour exécuter les essais en flux latéral comportant analytes multiples
WO2004001366A2 (fr) * 2001-12-21 2003-12-31 Kimberly-Clark Worldwide, Inc. Dispositif pour diagnostic
US7651841B2 (en) 2001-12-24 2010-01-26 Kimberly-Clark Worldwide, Inc. Polyelectrolytic internal calibration system of a flow-through assay
US7662643B2 (en) 2002-12-19 2010-02-16 Kimberly-Clark Worldwide, Inc. Reduction of the hook effect in membrane-based assay devices
US7670786B2 (en) 2002-08-27 2010-03-02 Kimberly-Clark Worldwide, Inc. Membrane-based assay devices
US7682817B2 (en) 2004-12-23 2010-03-23 Kimberly-Clark Worldwide, Inc. Microfluidic assay devices
US7695979B2 (en) 2002-05-03 2010-04-13 Kimberly-Clark Worldwide, Inc. Biomolecule diagnostic devices
US7713748B2 (en) 2003-11-21 2010-05-11 Kimberly-Clark Worldwide, Inc. Method of reducing the sensitivity of assay devices
US7771922B2 (en) 2002-05-03 2010-08-10 Kimberly-Clark Worldwide, Inc. Biomolecule diagnostic device
US7781172B2 (en) 2003-11-21 2010-08-24 Kimberly-Clark Worldwide, Inc. Method for extending the dynamic detection range of assay devices
US7786176B2 (en) 2005-07-29 2010-08-31 Kimberly-Clark Worldwide, Inc. Vaginal treatment composition containing xylitol
US7790471B2 (en) 2005-12-13 2010-09-07 Kimberly-Clark Worldwide, Inc. Metering technique for lateral flow assay devices
US7796266B2 (en) 2004-04-30 2010-09-14 Kimberly-Clark Worldwide, Inc. Optical detection system using electromagnetic radiation to detect presence or quantity of analyte
US7803319B2 (en) 2005-04-29 2010-09-28 Kimberly-Clark Worldwide, Inc. Metering technique for lateral flow assay devices
US7815854B2 (en) 2004-04-30 2010-10-19 Kimberly-Clark Worldwide, Inc. Electroluminescent illumination source for optical detection systems
US7829328B2 (en) 2003-04-03 2010-11-09 Kimberly-Clark Worldwide, Inc. Assay devices that utilize hollow particles
US7838258B2 (en) 2005-12-14 2010-11-23 Kimberly-Clark Worldwide, Inc. Meter strip and method for lateral flow assay devices
US7851209B2 (en) 2003-04-03 2010-12-14 Kimberly-Clark Worldwide, Inc. Reduction of the hook effect in assay devices
US7858384B2 (en) 2005-04-29 2010-12-28 Kimberly-Clark Worldwide, Inc. Flow control technique for assay devices
US7943395B2 (en) 2003-11-21 2011-05-17 Kimberly-Clark Worldwide, Inc. Extension of the dynamic detection range of assay devices
US7943089B2 (en) 2003-12-19 2011-05-17 Kimberly-Clark Worldwide, Inc. Laminated assay devices
US7964340B2 (en) 2004-06-30 2011-06-21 Kimberly-Clark Worldwide, Inc. One-step enzymatic and amine detection technique
US8535617B2 (en) 2007-11-30 2013-09-17 Kimberly-Clark Worldwide, Inc. Blood cell barrier for a lateral flow device

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GT200000033A (es) 2001-09-08
AR022779A1 (es) 2002-09-04
KR20010108273A (ko) 2001-12-07
EP1163513A1 (fr) 2001-12-19
RU2001126123A (ru) 2004-02-20
AU3504700A (en) 2000-09-14
SV2002000032A (es) 2002-08-26

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