WO2000050871A8 - Microstructures for use in biological assays and reactions - Google Patents

Microstructures for use in biological assays and reactions

Info

Publication number
WO2000050871A8
WO2000050871A8 PCT/US2000/005320 US0005320W WO0050871A8 WO 2000050871 A8 WO2000050871 A8 WO 2000050871A8 US 0005320 W US0005320 W US 0005320W WO 0050871 A8 WO0050871 A8 WO 0050871A8
Authority
WO
WIPO (PCT)
Prior art keywords
microstructures
reactions
assays
biological assays
analysis
Prior art date
Application number
PCT/US2000/005320
Other languages
French (fr)
Other versions
WO2000050871A1 (en
WO2000050871A9 (en
Inventor
Johannes Dapprich
Original Assignee
Orchid Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orchid Biosciences Inc filed Critical Orchid Biosciences Inc
Priority to AU35086/00A priority Critical patent/AU3508600A/en
Publication of WO2000050871A1 publication Critical patent/WO2000050871A1/en
Publication of WO2000050871A9 publication Critical patent/WO2000050871A9/en
Publication of WO2000050871A8 publication Critical patent/WO2000050871A8/en

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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

The present invention relates to microstructures (11) and microstructure assemblies (10) which are fabricated from a cast or molded polymer material, such as polydimethylsiloxane (PDMS). The invention describes and claims methods and compositions from which a variety of microstructures (11) may be prepared. The microstructures (11) are particularly useful for use in assays and reactions relating to biological assays, including applications in medical diagnostics. Such assays and reactions include, but are no means limited to, polynucleotide sequencing and analysis, including analysis of single nucleotide polymorphisms, and for polynucleotide amplification such as PCR.
PCT/US2000/005320 1999-02-26 2000-02-25 Microstructures for use in biological assays and reactions WO2000050871A1 (en)

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US60/122,020 1999-02-26

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WO2000050871A9 WO2000050871A9 (en) 2001-08-30
WO2000050871A8 true WO2000050871A8 (en) 2001-10-11

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