WO2000040758A3 - Enhanced sequencing by hybridization using pools of probes - Google Patents

Enhanced sequencing by hybridization using pools of probes Download PDF

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Publication number
WO2000040758A3
WO2000040758A3 PCT/US2000/000458 US0000458W WO0040758A3 WO 2000040758 A3 WO2000040758 A3 WO 2000040758A3 US 0000458 W US0000458 W US 0000458W WO 0040758 A3 WO0040758 A3 WO 0040758A3
Authority
WO
WIPO (PCT)
Prior art keywords
pools
probes
hybridization
sequencing
sbh
Prior art date
Application number
PCT/US2000/000458
Other languages
French (fr)
Other versions
WO2000040758A2 (en
Inventor
Radoje Drmanac
Snezana Drmanac
David Kita
Cory Cooke
Chongjun Xu
Original Assignee
Hyseq Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyseq Inc filed Critical Hyseq Inc
Priority to DE60042775T priority Critical patent/DE60042775D1/en
Priority to AT00903186T priority patent/ATE440148T1/en
Priority to AU24969/00A priority patent/AU2496900A/en
Priority to EP00903186A priority patent/EP1144684B1/en
Publication of WO2000040758A2 publication Critical patent/WO2000040758A2/en
Publication of WO2000040758A3 publication Critical patent/WO2000040758A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The invention provides methods for sequencing by hybridization (SBH) using pools of probes that allow greater efficiency in conducting SBH by reducing the number of separate measurements of hybridization signals required to identify each particular nucleotide in a target nucleic acid sequence. The invention also provides pools and sets of pools of probes, as well as methods of generating pools of probes.
PCT/US2000/000458 1999-01-06 2000-01-06 Enhanced sequencing by hybridization using pools of probes WO2000040758A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE60042775T DE60042775D1 (en) 1999-01-06 2000-01-06 IMPROVED SEQUENCING BY HYBRIDIZATION THROUGH THE USE OF PROBABLE MIXTURES
AT00903186T ATE440148T1 (en) 1999-01-06 2000-01-06 IMPROVED SEQUENCING VIA HYBRIDIZATION BY USING PROBE MIXTURES
AU24969/00A AU2496900A (en) 1999-01-06 2000-01-06 Enhanced sequencing by hybridization using pools of probes
EP00903186A EP1144684B1 (en) 1999-01-06 2000-01-06 Enhanced sequencing by hybridization using pools of probes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11528499P 1999-01-06 1999-01-06
US60/115,284 1999-01-06

Publications (2)

Publication Number Publication Date
WO2000040758A2 WO2000040758A2 (en) 2000-07-13
WO2000040758A3 true WO2000040758A3 (en) 2000-11-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/000458 WO2000040758A2 (en) 1999-01-06 2000-01-06 Enhanced sequencing by hybridization using pools of probes

Country Status (6)

Country Link
US (4) US6864052B1 (en)
EP (2) EP1144684B1 (en)
AT (1) ATE440148T1 (en)
AU (1) AU2496900A (en)
DE (1) DE60042775D1 (en)
WO (1) WO2000040758A2 (en)

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US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides

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