WO2000025136A1 - Method for gold deposition - Google Patents
Method for gold deposition Download PDFInfo
- Publication number
- WO2000025136A1 WO2000025136A1 PCT/IL1999/000570 IL9900570W WO0025136A1 WO 2000025136 A1 WO2000025136 A1 WO 2000025136A1 IL 9900570 W IL9900570 W IL 9900570W WO 0025136 A1 WO0025136 A1 WO 0025136A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gold
- substrate
- nucleation
- sites
- metal
- Prior art date
Links
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 144
- 239000010931 gold Substances 0.000 title claims abstract description 135
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 128
- 238000000034 method Methods 0.000 title claims abstract description 75
- 230000008021 deposition Effects 0.000 title description 29
- 230000006911 nucleation Effects 0.000 claims abstract description 129
- 239000000758 substrate Substances 0.000 claims abstract description 112
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 101
- 239000012491 analyte Substances 0.000 claims abstract description 56
- 238000010899 nucleation Methods 0.000 claims abstract description 56
- 229910052751 metal Inorganic materials 0.000 claims abstract description 55
- 239000002184 metal Substances 0.000 claims abstract description 55
- 239000000203 mixture Substances 0.000 claims abstract description 48
- 238000000151 deposition Methods 0.000 claims abstract description 38
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims description 62
- 230000027455 binding Effects 0.000 claims description 36
- 239000000126 substance Substances 0.000 claims description 31
- 108091034117 Oligonucleotide Proteins 0.000 claims description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims description 22
- 238000000926 separation method Methods 0.000 claims description 19
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N 1,4-Benzenediol Natural products OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims description 16
- 230000009870 specific binding Effects 0.000 claims description 16
- 230000015572 biosynthetic process Effects 0.000 claims description 15
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 14
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 14
- 239000002773 nucleotide Substances 0.000 claims description 13
- 125000003729 nucleotide group Chemical group 0.000 claims description 13
- 239000000427 antigen Substances 0.000 claims description 12
- 102000036639 antigens Human genes 0.000 claims description 12
- 108091007433 antigens Proteins 0.000 claims description 12
- 239000002923 metal particle Substances 0.000 claims description 11
- 239000001913 cellulose Substances 0.000 claims description 10
- 229920002678 cellulose Polymers 0.000 claims description 10
- 229960002685 biotin Drugs 0.000 claims description 9
- 239000011616 biotin Substances 0.000 claims description 9
- 238000003384 imaging method Methods 0.000 claims description 9
- DTMHTVJOHYTUHE-UHFFFAOYSA-N thiocyanogen Chemical compound N#CSSC#N DTMHTVJOHYTUHE-UHFFFAOYSA-N 0.000 claims description 9
- 108010090804 Streptavidin Proteins 0.000 claims description 7
- 235000020958 biotin Nutrition 0.000 claims description 7
- 230000000295 complement effect Effects 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 108090001008 Avidin Proteins 0.000 claims description 6
- 102000014914 Carrier Proteins Human genes 0.000 claims description 5
- 229920002101 Chitin Polymers 0.000 claims description 5
- 108020004635 Complementary DNA Proteins 0.000 claims description 5
- 102000004856 Lectins Human genes 0.000 claims description 5
- 108090001090 Lectins Proteins 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000011230 binding agent Substances 0.000 claims description 5
- 108091008324 binding proteins Proteins 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 5
- 238000010168 coupling process Methods 0.000 claims description 5
- 238000005859 coupling reaction Methods 0.000 claims description 5
- 239000002523 lectin Substances 0.000 claims description 5
- 239000003446 ligand Substances 0.000 claims description 5
- AZQWKYJCGOJGHM-UHFFFAOYSA-N para-benzoquinone Natural products O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 claims description 4
- 125000004151 quinonyl group Chemical group 0.000 claims description 2
- 125000000687 hydroquinonyl group Chemical group C1(O)=C(C=C(O)C=C1)* 0.000 claims 2
- 230000002708 enhancing effect Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 17
- 238000003556 assay Methods 0.000 description 14
- 239000000047 product Substances 0.000 description 11
- 238000009396 hybridization Methods 0.000 description 7
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 6
- 239000000084 colloidal system Substances 0.000 description 6
- 238000001465 metallisation Methods 0.000 description 6
- 229910052709 silver Inorganic materials 0.000 description 6
- 239000004332 silver Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 238000012800 visualization Methods 0.000 description 6
- 238000000018 DNA microarray Methods 0.000 description 5
- 238000005137 deposition process Methods 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 210000003463 organelle Anatomy 0.000 description 5
- 230000003197 catalytic effect Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- -1 silver ions Chemical class 0.000 description 4
- 230000002269 spontaneous effect Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003638 chemical reducing agent Substances 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 description 3
- 238000007794 visualization technique Methods 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 238000005234 chemical deposition Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000000399 optical microscopy Methods 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- RIOQSEWOXXDEQQ-UHFFFAOYSA-N triphenylphosphine Chemical compound C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1 RIOQSEWOXXDEQQ-UHFFFAOYSA-N 0.000 description 2
- VOLGAXAGEUPBDM-UHFFFAOYSA-N $l^{1}-oxidanylethane Chemical compound CC[O] VOLGAXAGEUPBDM-UHFFFAOYSA-N 0.000 description 1
- 108091028026 C-DNA Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000004630 atomic force microscopy Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000006352 cycloaddition reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 150000002343 gold Chemical class 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000010534 nucleophilic substitution reaction Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000005289 physical deposition Methods 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 238000007348 radical reaction Methods 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000003746 surface roughness Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 150000003573 thiols Chemical group 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004627 transmission electron microscopy Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
Definitions
- the metal to be deposited is provided in a form of a soluble complex or molecule, e.g. in an aqueous solution.
- nucleation centers may, for example, be a variety of metal complexes, clusters or small metal particles, deposited on or attached to the surface.
- a method for depositing gold at one or more sites on a substrate comprising:
- the substrate serves as a nucleation center for further gold deposition.
- the amount of the deposited gold at a deposition site can be controlled.
- the initial gold deposition may be at discrete sites on the substrate, by allowing the gold deposits to grow, following further deposition, discrete sites may combine to form one larger gold deposit encompassing the so combined sites.
- the nucleation centers are formed by chemical or physical deposition of nucleation center forming agent onto the one or more sites.
- such nucleation centers may be formed chemically at said one or more sites by a redox reaction in which metal ions, in particular silver ions, are oxidized into metallic silver.
- a redox reaction in which metal ions, in particular silver ions, are oxidized into metallic silver.
- the recognition group typically a recognition couple, consists of two or more substances with a binding affinity one to the other.
- the recognition group may be, but is not limited to, any one of the following couples: an antigen and an antibody or an antibody derivative with a complementary antigen-binding domain; sugar and a lectin; a receptor and a ligand; a nucleotide sequence and a complementary nucleotide sequence; a nucleotide sequence and its binding protein or synthetic binding agent; biotin and avidin or streptavidin; cellulose or chitin and cellulose binding domain.
- a treatment composition comprising Au (SCN) 2 " and HQ in an acidic medium is stable, meaning that gold remains as soluble species in solution and neither precipitates as solid metal nor is deposited on solid substrates other than nucleation centers.
- the pH of such a treatment composition is below about 6, the treatment composition is particularly stable.
- gold metal is deposited on sites of the substrate comprising nucleation centers.
- the rate of deposition of the gold metal may be controlled, for example, by controlling the pH of the treatment composition: increase in pH will increase the rate of gold deposition and a decrease in pH will decrease the deposition rate.
- Oligonucleotide chips have different capturing oligonucleotides deposited in different sites on the chip. Applying the above method may allow to simultaneously assay for a large number of different oligonucleotides in a sample.
- Fig. 6 is a schematic illustration of a method in accordance with an embodiment of the invention for imaging specific parts of a microscope specimen.
- Fig. 7 shows the application of the system in the detection of analytes, oligonucleotides with a specific sequence in this specific example, in a sample.
- the KAu (SCN) 2 solution is then mixed with a reducing agent, e.g. with about 0.055 M hydroquinone (HQ).
- a reducing agent e.g. with about 0.055 M hydroquinone (HQ).
- the reduction of the gold-containing complex by the hydroquinone is then thermodynamically favorable, but owing to the large activation energy and low pre-exponential factor, the kinetics is very slow.
- HQ hydroquinone
- the gold deposition process of the invention can be used for a variety of diverse applications, some examples of which will be illustrated below.
- FIG. 3 Another embodiment of an assay in accordance with the invention can be seen in Fig. 3.
- Other components of this assay are very similar to those of Fig. 2 and thus the same reference numeral shifted by one hundred (namely with a "1" prefix) to those used in Fig. 2 were employed in Fig. 3 to indicate like components.
- the target oligonucleotides attach to the surface in locations bearing the complementary sequence.
- Detection of hybridization products is usually done by following the fluorescence of labeled duplexes.
- the location of hybridization product indicates the sequence of the probe and fluorescence intensity indicates their surface concentration.
- a DNA-chip substrate 400 carries a plurality of DNA probe sites, three 402, 404 and 406 being exemplified, each one having a different nucleotide sequence.
- a sample S that may contain the target oligonucleotides 410 is treated in a way that all DNA species in solution become labeled by label 412, thus obtaining modified sample S' that may contain modified analyte (if analyte was present in S).
- Contacting modified sample S' with DNA-chip substrate 400 under appropriate conditions allows selective hybridization of DNA probes on chip to target oligonucleotides if present in modified sample S'.
- nucleation centers-forming agents 430 are introduced to the system under condition allowing their binding to labels 412 on labeled oligonucleotides if present at the different sites on chip. After washing, nucleation centers are present only at locations where hybridization between probes on chip and target from solution occurred. Visualization of hybridization is achieved by applying the gold deposition method of the invention, thus growing gold grains from the nucleation centers on-chip and detecting the gold 440 grains using different techniques such as optical techniques and SPM techniques (AFM, STM etc.).
Abstract
Description
Claims
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2348415A CA2348415C (en) | 1998-10-27 | 1999-10-27 | Method for gold deposition |
AU64859/99A AU759205B2 (en) | 1998-10-27 | 1999-10-27 | Method for gold deposition |
JP2000578659A JP4453856B2 (en) | 1998-10-27 | 1999-10-27 | Gold precipitation method |
US09/830,457 US7056748B1 (en) | 1998-10-27 | 1999-10-27 | Method for gold deposition |
EP99952775A EP1125128B1 (en) | 1998-10-27 | 1999-10-27 | Method for gold deposition |
DE69930285T DE69930285T2 (en) | 1998-10-27 | 1999-10-27 | METHOD OF GOLD DECISION |
US10/638,503 US7851149B2 (en) | 1998-10-27 | 2003-08-11 | Method for gold deposition |
US11/314,154 US7364920B2 (en) | 1999-10-27 | 2005-12-21 | Method for gold deposition |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL12677698A IL126776A (en) | 1998-10-27 | 1998-10-27 | Method for gold depositions |
IL126776 | 1998-10-27 |
Related Child Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09830457 A-371-Of-International | 1999-10-27 | ||
US09/830,457 A-371-Of-International US7056748B1 (en) | 1998-10-27 | 1999-10-27 | Method for gold deposition |
US10/638,503 Continuation US7851149B2 (en) | 1998-10-27 | 2003-08-11 | Method for gold deposition |
US11/314,154 Continuation US7364920B2 (en) | 1999-10-27 | 2005-12-21 | Method for gold deposition |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000025136A1 true WO2000025136A1 (en) | 2000-05-04 |
Family
ID=11072075
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IL1999/000570 WO2000025136A1 (en) | 1998-10-27 | 1999-10-27 | Method for gold deposition |
Country Status (12)
Country | Link |
---|---|
US (2) | US7056748B1 (en) |
EP (1) | EP1125128B1 (en) |
JP (1) | JP4453856B2 (en) |
CN (1) | CN1145028C (en) |
AT (1) | ATE320002T1 (en) |
AU (1) | AU759205B2 (en) |
CA (1) | CA2348415C (en) |
DE (1) | DE69930285T2 (en) |
ES (1) | ES2263289T3 (en) |
IL (1) | IL126776A (en) |
RU (1) | RU2242005C2 (en) |
WO (1) | WO2000025136A1 (en) |
Cited By (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6399303B1 (en) | 1999-04-07 | 2002-06-04 | Integrated Nano-Technologies, Llc | High resolution DNA detection methods and devices |
US6673548B2 (en) | 1996-07-29 | 2004-01-06 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6709825B2 (en) | 1996-07-29 | 2004-03-23 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6726847B2 (en) | 2000-12-06 | 2004-04-27 | Northwestern University | Silver stain removal by chemical etching and sonication |
US6750016B2 (en) | 1996-07-29 | 2004-06-15 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
EP1439392A2 (en) * | 2003-01-15 | 2004-07-21 | Agilent Technologies, Inc. | Biosensor systems and methods for determining the presence of biomolecules |
US6767702B2 (en) | 1996-07-29 | 2004-07-27 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6773884B2 (en) | 1996-07-29 | 2004-08-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6812334B1 (en) | 1996-07-29 | 2004-11-02 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
EP1479781A1 (en) * | 2003-05-21 | 2004-11-24 | Bayer Technology Services GmbH | Method for detecting nucleic acids |
WO2005001479A1 (en) * | 2003-06-23 | 2005-01-06 | Siemens Aktiengesellschaft | Capacitative biosensor element and method for detecting hybridization events |
WO2005003772A1 (en) * | 2003-07-03 | 2005-01-13 | Institut für Physikalische Hochtechnologie e.V. | Device and method for identifying an analyte |
US6946675B2 (en) | 1997-07-14 | 2005-09-20 | Technion Research & Development Foundation Ltd. | Microelectronic components and electronic networks comprising DNA |
US6984491B2 (en) | 1996-07-29 | 2006-01-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US7056748B1 (en) | 1998-10-27 | 2006-06-06 | Technion Research And Development Foundation Ltd. | Method for gold deposition |
US7186814B2 (en) | 2001-11-09 | 2007-03-06 | Nanosphere, Inc. | Bioconjugate-nanoparticle probes |
US7323309B2 (en) | 2000-03-28 | 2008-01-29 | Northwestern University | Bio-barcodes based on oligonucleotide-modified particles |
US7364920B2 (en) | 1999-10-27 | 2008-04-29 | Technion Research And Development Foundation Ltd. | Method for gold deposition |
WO2009068862A1 (en) * | 2007-11-26 | 2009-06-04 | The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government | Electrochemical detection using silver nanoparticle labelled antibodies |
ITTO20080632A1 (en) * | 2008-08-12 | 2010-02-13 | Consiglio Naz Delle Ricerche Infm Istituto | PROCEDURE FOR MICROMETRIC AND NANOMETRIC SCALE CONFIGURATION OF METALLIC LAYERS ON A SUBSTRATE |
US8927299B2 (en) | 2008-07-10 | 2015-01-06 | The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government | Sample carrier for effecting chemical assays |
US10232365B2 (en) | 2012-09-28 | 2019-03-19 | Agplus Diagnostics Ltd | Test device and sample carrier |
CN115951070A (en) * | 2023-01-19 | 2023-04-11 | 北京青莲百奥生物科技有限公司 | Method for detecting protein in sample |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL124322A (en) * | 1998-05-04 | 2002-05-23 | Technion Res & Dev Foundation | Detection of an entity in a sample |
RU2578977C1 (en) * | 2015-05-12 | 2016-03-27 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт глазных болезней" | Method of preparing biological sample for analysis by scanning electron microscopy |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3032436A (en) * | 1960-11-18 | 1962-05-01 | Metal Proc Co Inc | Method and composition for plating by chemical reduction |
EP0426300A1 (en) * | 1989-09-29 | 1991-05-08 | Ortho Diagnostic Systems Inc. | Method of producing a reagent containing a narrow distribution of colloidal particles of a selected size and the use thereof |
US5202151A (en) * | 1985-10-14 | 1993-04-13 | Hitachi, Ltd. | Electroless gold plating solution, method of plating with gold by using the same, and electronic device plated with gold by using the same |
JPH05105444A (en) * | 1991-09-25 | 1993-04-27 | Mitsubishi Materials Corp | Manufacture of spheroidal gold fine grain |
US5284748A (en) * | 1986-03-25 | 1994-02-08 | Immunotronics, Inc. | Method for electrical detection of a binding reaction |
Family Cites Families (79)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1343642A (en) * | 1971-04-28 | 1974-01-16 | May & Baker Ltd | Zinc sulphided pigments |
US3833894A (en) | 1973-06-20 | 1974-09-03 | Ibm | Organic memory device |
US4314821A (en) | 1979-04-09 | 1982-02-09 | Minnesota Mining And Manufacturing Company | Sandwich immunoassay using piezoelectric oscillator |
US4323641A (en) * | 1980-08-15 | 1982-04-06 | The United States Of America As Represented By The Secretary Of The Navy | Photographic image enhancement by a gold-toning neutron-activation process |
US5241060A (en) | 1982-06-23 | 1993-08-31 | Enzo Diagnostics, Inc. | Base moiety-labeled detectable nucleatide |
GB8331514D0 (en) | 1983-11-25 | 1984-01-04 | Janssen Pharmaceutica Nv | Visualization method |
US4822566A (en) | 1985-11-19 | 1989-04-18 | The Johns Hopkins University | Optimized capacitive sensor for chemical analysis and measurement |
US4794089A (en) | 1986-03-25 | 1988-12-27 | Midwest Research Microscopy, Inc. | Method for electronic detection of a binding reaction |
US5491098A (en) * | 1987-03-09 | 1996-02-13 | Janssen Pharmaceutica N.V. | Method for depositing metal particles on a marker |
US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
GB8800702D0 (en) | 1988-01-13 | 1988-02-10 | Nycomed As | Test method & reagent kit therefor |
US5063417A (en) | 1988-07-18 | 1991-11-05 | California Institute Of Technology | Molecular shift register based on electron transfer |
US4995402A (en) | 1988-10-12 | 1991-02-26 | Thorne, Smith, Astill Technologies, Inc. | Medical droplet whole blood and like monitoring |
CA2002660A1 (en) | 1988-11-10 | 1990-05-10 | Susan J. Mroczkowski | Method for electrical detection of a binding reaction |
US5089545A (en) | 1989-02-12 | 1992-02-18 | Biotech International, Inc. | Switching and memory elements from polyamino acids and the method of their assembly |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
DE3924454A1 (en) | 1989-07-24 | 1991-02-07 | Cornelis P Prof Dr Hollenberg | THE APPLICATION OF DNA AND DNA TECHNOLOGY FOR THE CONSTRUCTION OF NETWORKS FOR USE IN CHIP CONSTRUCTION AND CHIP PRODUCTION (DNA CHIPS) |
CA2034266A1 (en) | 1990-02-27 | 1991-08-28 | Paul A. D'orazio | Thick film reagent spreading and reagent immobilization layer |
HU218095B (en) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Process for reducing transitional contaminations in amplification processes |
US5714327A (en) | 1990-07-19 | 1998-02-03 | Kreatech Diagnostics | Platinum-containing compounds, methods for their preparation and applications thereof |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
JP3064001B2 (en) | 1990-10-09 | 2000-07-12 | 株式会社アドバンス | Molecular fixation device |
US5294369A (en) | 1990-12-05 | 1994-03-15 | Akzo N.V. | Ligand gold bonding |
DE69109653T2 (en) * | 1991-01-15 | 1996-01-11 | Agfa Gevaert Nv | Process for the photographic production of silver images. |
JP2778304B2 (en) | 1991-09-17 | 1998-07-23 | 三菱電機株式会社 | Organic electronic device materials |
US6051380A (en) | 1993-11-01 | 2000-04-18 | Nanogen, Inc. | Methods and procedures for molecular biological analysis and diagnostics |
US5787032A (en) | 1991-11-07 | 1998-07-28 | Nanogen | Deoxyribonucleic acid(DNA) optical storage using non-radiative energy transfer between a donor group, an acceptor group and a quencher group |
IL103674A0 (en) | 1991-11-19 | 1993-04-04 | Houston Advanced Res Center | Method and apparatus for molecule detection |
US5846708A (en) | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
GB9210176D0 (en) | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Chemical method |
ATE251361T1 (en) | 1992-06-01 | 2003-10-15 | Univ Yale | ELECTRONIC CIRCUIT IN THE NANO SCALE AND METHOD FOR THE PRODUCTION THEREOF |
US5331183A (en) | 1992-08-17 | 1994-07-19 | The Regents Of The University Of California | Conjugated polymer - acceptor heterojunctions; diodes, photodiodes, and photovoltaic cells |
US5312527A (en) | 1992-10-06 | 1994-05-17 | Concordia University | Voltammetric sequence-selective sensor for target polynucleotide sequences |
CN1122138A (en) | 1993-01-21 | 1996-05-08 | 海布里顿公司 | Foldback triplex-forming oligonucleotides |
US5384265A (en) * | 1993-03-26 | 1995-01-24 | Geo-Centers, Inc. | Biomolecules bound to catalytic inorganic particles, immunoassays using the same |
US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
US5837546A (en) | 1993-08-24 | 1998-11-17 | Metrika, Inc. | Electronic assay device and method |
US5824473A (en) | 1993-12-10 | 1998-10-20 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6071699A (en) | 1996-06-07 | 2000-06-06 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5563424A (en) | 1994-03-24 | 1996-10-08 | Uniax Corporation | Polymer grid triodes |
US5871918A (en) | 1996-06-20 | 1999-02-16 | The University Of North Carolina At Chapel Hill | Electrochemical detection of nucleic acid hybridization |
CN1661115A (en) * | 1995-03-10 | 2005-08-31 | 梅索磅秤技术有限公司 | Multi-array, multi-specific electrochemiluminescence testing |
US5679519A (en) * | 1995-05-09 | 1997-10-21 | Oprandy; John J. | Multi-label complex for enhanced sensitivity in electrochemiluminescence assay |
US5614832A (en) | 1995-06-02 | 1997-03-25 | International Business Machines Corporation | High voltage protected ohmmeter |
US5595878A (en) * | 1995-06-02 | 1997-01-21 | Boron Biologicals, Inc. | Detection of biopolymers and biooligomers with boron hydride labels |
JPH0973780A (en) | 1995-09-01 | 1997-03-18 | Toshiba Corp | Semiconductor integrated circuit |
US5888370A (en) | 1996-02-23 | 1999-03-30 | Board Of Regents, The University Of Texas System | Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation |
AU734086B2 (en) | 1996-05-22 | 2001-05-31 | Australian Membrane And Biotechnology Research Institute | Nucleic acid sensor |
DE19622628A1 (en) | 1996-06-05 | 1997-12-11 | Boehringer Mannheim Gmbh | Stabilization of metal conjugates |
US6582921B2 (en) | 1996-07-29 | 2003-06-24 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses thereof |
US6750016B2 (en) | 1996-07-29 | 2004-06-15 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6506564B1 (en) | 1996-07-29 | 2003-01-14 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6984491B2 (en) | 1996-07-29 | 2006-01-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
JPH1068730A (en) | 1996-08-27 | 1998-03-10 | Wako Pure Chem Ind Ltd | Test tool for immunochromatography |
US5787332A (en) | 1996-09-26 | 1998-07-28 | Fansteel Inc. | Process for recovering tantalum and/or niobium compounds from composites containing a variety of metal compounds |
US5874046A (en) | 1996-10-30 | 1999-02-23 | Raytheon Company | Biological warfare agent sensor system employing ruthenium-terminated oligonucleotides complementary to target live agent DNA sequences |
US6306584B1 (en) | 1997-01-21 | 2001-10-23 | President And Fellows Of Harvard College | Electronic-property probing of biological molecules at surfaces |
AU748939B2 (en) | 1997-02-20 | 2002-06-13 | Regents Of The University Of California, The | Plasmon resonant particles, methods and apparatus |
US5946550A (en) | 1997-03-14 | 1999-08-31 | University Of Connecticut | Self-assembled semiconductor and method of making same |
US6391558B1 (en) | 1997-03-18 | 2002-05-21 | Andcare, Inc. | Electrochemical detection of nucleic acid sequences |
US6210880B1 (en) | 1997-09-19 | 2001-04-03 | Third Wave Technologies, Inc. | Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides |
AU7697998A (en) | 1997-05-27 | 1998-12-30 | State of Oregon Acting By and Through The State Board of Higher Education On Behalf of The Unive, The | Scaffold-organized metal, alloy, semiconductor and/or magnetic clusters and electronic devices made using such clusters |
IL121312A (en) | 1997-07-14 | 2001-09-13 | Technion Res & Dev Foundation | Microelectronic components, their fabrication and electronic networks comprising them |
US20020025552A1 (en) | 1998-01-06 | 2002-02-28 | Institut Pasteur | Screening interactor molecules with whole genome oligonucleotide or polynucleotide arrays |
WO1999036573A1 (en) | 1998-01-20 | 1999-07-22 | Schichman Steven A | Detection of genetic information |
US6060023A (en) | 1998-03-31 | 2000-05-09 | Motorola, Inc. | Molecular sensing apparatus |
IL124322A (en) | 1998-05-04 | 2002-05-23 | Technion Res & Dev Foundation | Detection of an entity in a sample |
EP1080229A4 (en) | 1998-05-20 | 2005-07-13 | Nano-Technologies L Integrated | Chemically assembled nano-scale devices |
US6093370A (en) | 1998-06-11 | 2000-07-25 | Hitachi, Ltd. | Polynucleotide separation method and apparatus therefor |
US6171870B1 (en) * | 1998-08-06 | 2001-01-09 | Spectral Diagnostics, Inc. | Analytical test device and method for use in medical diagnoses |
IL126776A (en) | 1998-10-27 | 2001-04-30 | Technion Res & Dev Foundation | Method for gold depositions |
US6333146B1 (en) * | 1999-03-10 | 2001-12-25 | Fuji Photo Film Co., Ltd. | Methine compound and silver halide photographic material containing the same |
WO2000060125A2 (en) | 1999-04-07 | 2000-10-12 | Dennis Michael Connolly | High resolution dna detection methods and devices |
US7364920B2 (en) * | 1999-10-27 | 2008-04-29 | Technion Research And Development Foundation Ltd. | Method for gold deposition |
WO2002004681A2 (en) * | 2000-07-11 | 2002-01-17 | Northwestern University | Method of detection by enhancement of silver staining |
US6670113B2 (en) * | 2001-03-30 | 2003-12-30 | Nanoprobes | Enzymatic deposition and alteration of metals |
US7266855B2 (en) * | 2002-06-04 | 2007-09-11 | Qingping Zhuan | Electric toothbrush |
US7488607B2 (en) * | 2003-09-30 | 2009-02-10 | Agilent Technologies, Inc. | Electronically readable microarray with electronic addressing function |
US20060014083A1 (en) * | 2004-03-01 | 2006-01-19 | University Of Washington | Methods and systems for fabricating electronic and/or microfluidic structures on elastomeric substrates |
-
1998
- 1998-10-27 IL IL12677698A patent/IL126776A/en not_active IP Right Cessation
-
1999
- 1999-10-27 DE DE69930285T patent/DE69930285T2/en not_active Expired - Lifetime
- 1999-10-27 AU AU64859/99A patent/AU759205B2/en not_active Ceased
- 1999-10-27 CA CA2348415A patent/CA2348415C/en not_active Expired - Fee Related
- 1999-10-27 EP EP99952775A patent/EP1125128B1/en not_active Expired - Lifetime
- 1999-10-27 WO PCT/IL1999/000570 patent/WO2000025136A1/en active IP Right Grant
- 1999-10-27 JP JP2000578659A patent/JP4453856B2/en not_active Expired - Fee Related
- 1999-10-27 RU RU2001114202/15A patent/RU2242005C2/en not_active IP Right Cessation
- 1999-10-27 CN CNB998148814A patent/CN1145028C/en not_active Expired - Fee Related
- 1999-10-27 ES ES99952775T patent/ES2263289T3/en not_active Expired - Lifetime
- 1999-10-27 AT AT99952775T patent/ATE320002T1/en not_active IP Right Cessation
- 1999-10-27 US US09/830,457 patent/US7056748B1/en not_active Expired - Lifetime
-
2003
- 2003-08-11 US US10/638,503 patent/US7851149B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3032436A (en) * | 1960-11-18 | 1962-05-01 | Metal Proc Co Inc | Method and composition for plating by chemical reduction |
US5202151A (en) * | 1985-10-14 | 1993-04-13 | Hitachi, Ltd. | Electroless gold plating solution, method of plating with gold by using the same, and electronic device plated with gold by using the same |
US5284748A (en) * | 1986-03-25 | 1994-02-08 | Immunotronics, Inc. | Method for electrical detection of a binding reaction |
EP0426300A1 (en) * | 1989-09-29 | 1991-05-08 | Ortho Diagnostic Systems Inc. | Method of producing a reagent containing a narrow distribution of colloidal particles of a selected size and the use thereof |
JPH05105444A (en) * | 1991-09-25 | 1993-04-27 | Mitsubishi Materials Corp | Manufacture of spheroidal gold fine grain |
Non-Patent Citations (1)
Title |
---|
DATABASE WPI Week 199321, Derwent World Patents Index; AN 1993-172471, XP002129601, OTAKI HIROAKI: "Spherical fine gold particles prodn. for ceramics or electronic industry" * |
Cited By (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6828432B2 (en) | 1996-07-29 | 2004-12-07 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6720147B2 (en) | 1996-07-29 | 2004-04-13 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6984491B2 (en) | 1996-07-29 | 2006-01-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6677122B2 (en) | 1996-07-29 | 2004-01-13 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6682895B2 (en) | 1996-07-29 | 2004-01-27 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6709825B2 (en) | 1996-07-29 | 2004-03-23 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6720411B2 (en) | 1996-07-29 | 2004-04-13 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6902895B2 (en) | 1996-07-29 | 2005-06-07 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6903207B2 (en) | 1996-07-29 | 2005-06-07 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6730269B2 (en) | 1996-07-29 | 2004-05-04 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6740491B2 (en) | 1996-07-29 | 2004-05-25 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6750016B2 (en) | 1996-07-29 | 2004-06-15 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6878814B2 (en) | 1996-07-29 | 2005-04-12 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6673548B2 (en) | 1996-07-29 | 2004-01-06 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6767702B2 (en) | 1996-07-29 | 2004-07-27 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6773884B2 (en) | 1996-07-29 | 2004-08-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6777186B2 (en) | 1996-07-29 | 2004-08-17 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6812334B1 (en) | 1996-07-29 | 2004-11-02 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6818753B2 (en) | 1996-07-29 | 2004-11-16 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6759199B2 (en) | 1996-07-29 | 2004-07-06 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
US6946675B2 (en) | 1997-07-14 | 2005-09-20 | Technion Research & Development Foundation Ltd. | Microelectronic components and electronic networks comprising DNA |
US7056748B1 (en) | 1998-10-27 | 2006-06-06 | Technion Research And Development Foundation Ltd. | Method for gold deposition |
US7851149B2 (en) | 1998-10-27 | 2010-12-14 | Erez Braun | Method for gold deposition |
US6593090B2 (en) | 1999-04-07 | 2003-07-15 | Integrated Nano-Technologies, Llc | High resolution DNA detection methods and devices |
US6399303B1 (en) | 1999-04-07 | 2002-06-04 | Integrated Nano-Technologies, Llc | High resolution DNA detection methods and devices |
US7364920B2 (en) | 1999-10-27 | 2008-04-29 | Technion Research And Development Foundation Ltd. | Method for gold deposition |
US7323309B2 (en) | 2000-03-28 | 2008-01-29 | Northwestern University | Bio-barcodes based on oligonucleotide-modified particles |
US6726847B2 (en) | 2000-12-06 | 2004-04-27 | Northwestern University | Silver stain removal by chemical etching and sonication |
US7186814B2 (en) | 2001-11-09 | 2007-03-06 | Nanosphere, Inc. | Bioconjugate-nanoparticle probes |
EP1439392A3 (en) * | 2003-01-15 | 2005-07-27 | Agilent Technologies, Inc. | Biosensor systems and methods for determining the presence of biomolecules |
EP1439392A2 (en) * | 2003-01-15 | 2004-07-21 | Agilent Technologies, Inc. | Biosensor systems and methods for determining the presence of biomolecules |
EP1479781A1 (en) * | 2003-05-21 | 2004-11-24 | Bayer Technology Services GmbH | Method for detecting nucleic acids |
WO2005001479A1 (en) * | 2003-06-23 | 2005-01-06 | Siemens Aktiengesellschaft | Capacitative biosensor element and method for detecting hybridization events |
WO2005003772A1 (en) * | 2003-07-03 | 2005-01-13 | Institut für Physikalische Hochtechnologie e.V. | Device and method for identifying an analyte |
GB2458420B (en) * | 2007-11-26 | 2009-10-28 | Sec Dep For Innovation Univers | Electrochemical detection of a metal-labelled analyte |
GB2458420A (en) * | 2007-11-26 | 2009-09-23 | Sec Dep For Innovation Univers | Electrochemical detection using silver nanoparticle labelled antibodies |
WO2009068862A1 (en) * | 2007-11-26 | 2009-06-04 | The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government | Electrochemical detection using silver nanoparticle labelled antibodies |
US8337692B2 (en) | 2007-11-26 | 2012-12-25 | The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government | Electrochemical detection of a metal—labelled analyte |
US8927299B2 (en) | 2008-07-10 | 2015-01-06 | The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government | Sample carrier for effecting chemical assays |
ITTO20080632A1 (en) * | 2008-08-12 | 2010-02-13 | Consiglio Naz Delle Ricerche Infm Istituto | PROCEDURE FOR MICROMETRIC AND NANOMETRIC SCALE CONFIGURATION OF METALLIC LAYERS ON A SUBSTRATE |
US10232365B2 (en) | 2012-09-28 | 2019-03-19 | Agplus Diagnostics Ltd | Test device and sample carrier |
CN115951070A (en) * | 2023-01-19 | 2023-04-11 | 北京青莲百奥生物科技有限公司 | Method for detecting protein in sample |
Also Published As
Publication number | Publication date |
---|---|
RU2242005C2 (en) | 2004-12-10 |
JP2002528098A (en) | 2002-09-03 |
EP1125128B1 (en) | 2006-03-08 |
AU6485999A (en) | 2000-05-15 |
ATE320002T1 (en) | 2006-03-15 |
DE69930285T2 (en) | 2006-12-07 |
DE69930285D1 (en) | 2006-05-04 |
CN1145028C (en) | 2004-04-07 |
IL126776A0 (en) | 1999-08-17 |
EP1125128A1 (en) | 2001-08-22 |
US20040033626A1 (en) | 2004-02-19 |
CA2348415A1 (en) | 2000-05-04 |
ES2263289T3 (en) | 2006-12-01 |
US7851149B2 (en) | 2010-12-14 |
CN1331800A (en) | 2002-01-16 |
IL126776A (en) | 2001-04-30 |
CA2348415C (en) | 2010-09-07 |
JP4453856B2 (en) | 2010-04-21 |
AU759205B2 (en) | 2003-04-10 |
US7056748B1 (en) | 2006-06-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1125128B1 (en) | Method for gold deposition | |
EP1075656B1 (en) | Detection of a target in a sample | |
US7985539B2 (en) | Nanoparticle probes with raman spectroscopic fingerprints for analyte detection | |
US6878539B1 (en) | Affinity sensor for detecting specific molecular binding events and use thereof | |
IE69371B1 (en) | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets | |
WO2008090229A1 (en) | Detecting analytes using both an optical and an electrical measurement method | |
US20030087277A1 (en) | Means and methods for detection of binding of members of specific binding pairs | |
EP1740951A1 (en) | Method and kit for detecting components in a sample | |
KR101075760B1 (en) | Apparatus and methods for detecting dna in biological samples | |
US7364920B2 (en) | Method for gold deposition | |
US20030203394A1 (en) | Detection of a target in a sample | |
US20050255515A1 (en) | Biomolecular sensors and detection methods utilizing photoinduced charge separation | |
JP2009517690A (en) | Electrochemical methods for detecting analytes | |
MXPA01004280A (en) | Method for gold deposition | |
US20050122386A1 (en) | Electrochemical signal amplification by repetitive activation of surface nucleation points |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 99814881.4 Country of ref document: CN |
|
ENP | Entry into the national phase |
Ref document number: 1999 64859 Country of ref document: AU Kind code of ref document: A |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
ENP | Entry into the national phase |
Ref document number: 2348415 Country of ref document: CA Ref document number: 2348415 Country of ref document: CA Kind code of ref document: A Ref document number: 2000 578659 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: PA/a/2001/004280 Country of ref document: MX |
|
WWE | Wipo information: entry into national phase |
Ref document number: 64859/99 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1999952775 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: IN/PCT/2001/542/KOL Country of ref document: IN |
|
WWP | Wipo information: published in national office |
Ref document number: 1999952775 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09830457 Country of ref document: US |
|
WWG | Wipo information: grant in national office |
Ref document number: 64859/99 Country of ref document: AU |
|
WWG | Wipo information: grant in national office |
Ref document number: 1999952775 Country of ref document: EP |