WO2000023615A3 - Method for extracting quantitative information relating to an influence on a cellular response - Google Patents

Method for extracting quantitative information relating to an influence on a cellular response Download PDF

Info

Publication number
WO2000023615A3
WO2000023615A3 PCT/DK1999/000562 DK9900562W WO0023615A3 WO 2000023615 A3 WO2000023615 A3 WO 2000023615A3 DK 9900562 W DK9900562 W DK 9900562W WO 0023615 A3 WO0023615 A3 WO 0023615A3
Authority
WO
WIPO (PCT)
Prior art keywords
influence
cellular response
information relating
quantitative information
extracting quantitative
Prior art date
Application number
PCT/DK1999/000562
Other languages
French (fr)
Other versions
WO2000023615A2 (en
Inventor
Per O G Arkhammar
Bernard Robert Terry
Kurt Marshall Scudder
Sara Petersen Bjoern
Ole Thastrup
Grith Hagel
Original Assignee
Bioimage As
Per O G Arkhammar
Bernard Robert Terry
Kurt Marshall Scudder
Sara Petersen Bjoern
Ole Thastrup
Grith Hagel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioimage As, Per O G Arkhammar, Bernard Robert Terry, Kurt Marshall Scudder, Sara Petersen Bjoern, Ole Thastrup, Grith Hagel filed Critical Bioimage As
Priority to AU61895/99A priority Critical patent/AU6189599A/en
Priority to JP2000577322A priority patent/JP2002527761A/en
Priority to EP99948731A priority patent/EP1121593A2/en
Publication of WO2000023615A2 publication Critical patent/WO2000023615A2/en
Publication of WO2000023615A3 publication Critical patent/WO2000023615A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases

Abstract

An improved method and tools for quantifying the effect of an influence on cellular response is described. In particular, an improved method is described for detecting intracellular translocation or redistribution of biologically active polypeptides. The invention also describes several ways of contacting the cells with a substance influencing a cellular response and extracting quantitative information relating to the response in a highly parallel fashion. The method may be used as a very efficient procedure for testing or discovering the influence of a substance on a physiological process using commercially available parallel, high volume assay techniques, for example in connection with screening for new drugs, testing of substances for toxicity, and identifying drug targets for known or novel drugs.
PCT/DK1999/000562 1998-10-15 1999-10-15 Method for extracting quantitative information relating to an influence on a cellular response WO2000023615A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU61895/99A AU6189599A (en) 1998-10-15 1999-10-15 An improved method for extracting quantitative information relating to an influence on a cellular response
JP2000577322A JP2002527761A (en) 1998-10-15 1999-10-15 An improved method for obtaining quantitative information on effects on cellular responses
EP99948731A EP1121593A2 (en) 1998-10-15 1999-10-15 Method for extracting quantitative information relating to an influence on a cellular response

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199801320 1998-10-15
DKPA199801320 1998-10-15

Publications (2)

Publication Number Publication Date
WO2000023615A2 WO2000023615A2 (en) 2000-04-27
WO2000023615A3 true WO2000023615A3 (en) 2001-03-29

Family

ID=8103540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1999/000562 WO2000023615A2 (en) 1998-10-15 1999-10-15 Method for extracting quantitative information relating to an influence on a cellular response

Country Status (4)

Country Link
EP (1) EP1121593A2 (en)
JP (1) JP2002527761A (en)
AU (1) AU6189599A (en)
WO (1) WO2000023615A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7236888B2 (en) 1998-03-06 2007-06-26 The Regents Of The University Of California Method to measure the activation state of signaling pathways in cells
US7282347B2 (en) 2000-07-04 2007-10-16 Fisher Bioimage Aps Method for extracting quantitative information relating to interactions between cellular components
EP1333099B1 (en) * 2000-11-06 2008-10-29 Ajinomoto Co., Inc. Method of searching for gene encoding nuclear transport protein
WO2002042732A2 (en) * 2000-11-22 2002-05-30 The Regents Of The University Of California A method to measure the activation state of signaling pathways in cells
US7033790B2 (en) 2001-04-03 2006-04-25 Curagen Corporation Proteins and nucleic acids encoding same
US20030039383A1 (en) * 2001-06-26 2003-02-27 Bio-Rad Laboratories, Inc. Flat field correction of two-dimensional biochemical assay images
WO2003012068A2 (en) 2001-08-01 2003-02-13 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
GB0203924D0 (en) * 2002-02-19 2002-04-03 Univ Bristol Screening method
FR3010521B1 (en) * 2013-09-12 2015-10-09 Luxor SIMPLIFIED FLUORIMETRIC PROCESS FOR EVALUATING THE INFLUENCE OF A CONDITION ON A BIOLOGICAL SAMPLE AND ITS APPLICATIONS
JP2015075477A (en) * 2013-10-11 2015-04-20 独立行政法人産業技術総合研究所 Stress luminescence evaluation system and stress luminescence evaluation method
CN105551009B (en) * 2015-12-17 2018-03-16 哈尔滨工业大学 A kind of image interfusion method based on continuous Terahertz cofocus scanning polarization imaging
CN106770102A (en) * 2016-12-05 2017-05-31 百奥森（江苏）食品安全科技有限公司 A kind of streptomysin detection method
CN113740531B (en) * 2021-09-01 2023-02-03 陕西脉元生物科技有限公司 Cell for reducing fluorescence nonspecific color development, detection product based on cell and application of cell in detecting autoantibody

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991001305A1 (en) * 1989-07-22 1991-02-07 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
WO1994023039A1 (en) * 1993-04-07 1994-10-13 The Institute Of Cancer Research: Royal Cancer Hospital Methods for screening of substances for therapeutic activity and yeast for use therein
WO1995007463A1 (en) * 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
WO1996003649A1 (en) * 1994-07-22 1996-02-08 The University Of North Carolina At Chapel Hill Src SH3 BINDING PEPTIDES AND METHODS OF ISOLATING AND USING SAME
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
WO1997011094A1 (en) * 1995-09-22 1997-03-27 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
WO1997020931A1 (en) * 1995-12-08 1997-06-12 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Method and compositions for monitoring dna binding molecules in living cells
WO1997030074A1 (en) * 1996-02-16 1997-08-21 Cytogen Corporation Isolation and use of sh3 binding peptides
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998030715A1 (en) * 1997-01-07 1998-07-16 California Institute Of Technology Optical sensors of cell signaling
WO1998045704A2 (en) * 1997-04-07 1998-10-15 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991001305A1 (en) * 1989-07-22 1991-02-07 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
WO1994023039A1 (en) * 1993-04-07 1994-10-13 The Institute Of Cancer Research: Royal Cancer Hospital Methods for screening of substances for therapeutic activity and yeast for use therein
WO1995007463A1 (en) * 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
WO1996003649A1 (en) * 1994-07-22 1996-02-08 The University Of North Carolina At Chapel Hill Src SH3 BINDING PEPTIDES AND METHODS OF ISOLATING AND USING SAME
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
WO1997011094A1 (en) * 1995-09-22 1997-03-27 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
WO1997020931A1 (en) * 1995-12-08 1997-06-12 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Method and compositions for monitoring dna binding molecules in living cells
WO1997030074A1 (en) * 1996-02-16 1997-08-21 Cytogen Corporation Isolation and use of sh3 binding peptides
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998030715A1 (en) * 1997-01-07 1998-07-16 California Institute Of Technology Optical sensors of cell signaling
WO1998045704A2 (en) * 1997-04-07 1998-10-15 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CAREY K L ET AL: "EVIDENCE USING A GREEN FLUORESCENT PROTEIN-GLUCOCORTICOID RECEPTOR CHIMERA THAT THE RAN/TC4 GTPASE MEDIATES AN ESSENTIAL FUNCTION INDEPENDENT OF NUCLEAR PROTEIN IMPORT", THE JOURNAL OF CELL BIOLOGY,US,ROCKEFELLER UNIVERSITY PRESS, vol. 133, no. 5, pages 985-996, XP000670316, ISSN: 0021-9525 *
GERISCH ET AL: "Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostelium cells monitored using a green fluorescent protein-coronin fusion protein", CURRENT BIOLOGY,GB,CURRENT SCIENCE,, vol. 5, no. 11, pages 1280-1285, XP002089510, ISSN: 0960-9822 *
HAN HTUN ET AL: "VISUALIZATION OF GLUCOCORTICOID RECEPTOR TRANSLOCATION AND INTRANUCLEAR ORGANIZATION IN LIVING CELLS WITH A GREEN FLUORESCENT PROTEIN CHIMERA", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 93, no. 10, pages 4845-4850, XP002029560, ISSN: 0027-8424 *
OGAWA H ET AL: "LOCALIZATION, TRAFFICKING, AND TEMPERATURE-DEPENDENCE OF THE AEQUOREA GREEN FLUORESCENT PROTEIN IN CULTURES VERTEBRATE CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 92, no. 25, pages 11899-11903, XP002029556, ISSN: 0027-8424 *
SAKAI ET AL: "Translocation of protein kinase C-gamma and epsilon - Direct visualization in living cells using fusion protein with green fluorescent protein", THE JOURNAL OF CELL BIOLOGY,US,ROCKEFELLER UNIVERSITY PRESS, XP002078902, ISSN: 0021-9525 *
SCHMIDT ET AL: "Dynamic analysis of alpha-PKC-GFP chimera translocation events in smooth muscle with ultra-high speed 3D fluorescence microscopy", FASEB JOURNAL,US,FED. OF AMERICAN SOC. FOR EXPERIMENTAL BIOLOGY, BETHESDA, MD, vol. 3, no. 11, pages A505, XP002077257, ISSN: 0892-6638 *
SIDOROVA ET AL: "Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization", MOLECULAR BIOLOGY OF THE CELL,US,BETHESDA, MD, vol. 6, no. 12, pages 1641-1658, XP002089512, ISSN: 1059-1524 *
TODA ET AL: "The fission yeast sts5+ gene is required for maintenance of growth polarity and functionally interacts with protein kinase C and an osmosensing MAP kinase pathway", JOURNAL OF CELL SCIENCE,GB,ESSEX, vol. 109, no. 9, pages 2331-2342, XP002090292 *
WEBB ET AL: "Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis", JOURNAL OF BACTERIOLOGY,US,WASHINGTON, DC, vol. 177, no. 20, pages 5906-5911, XP002089513, ISSN: 0021-9193 *
WESTPHAL ET AL: "Microfilament dynamics during cell movement and chemotaxis monitored using a GFP - actin fusion protein", CURRENT BIOLOGY,GB,CURRENT SCIENCE,, vol. 7, no. 3, pages 176-183, XP002090291, ISSN: 0960-9822 *

Also Published As

Publication number Publication date
AU6189599A (en) 2000-05-08
EP1121593A2 (en) 2001-08-08
JP2002527761A (en) 2002-08-27
WO2000023615A2 (en) 2000-04-27

Similar Documents

Publication Publication Date Title
WO2000023615A3 (en) Method for extracting quantitative information relating to an influence on a cellular response
TWI256975B (en) Compositions containing a urea derivative dye for detecting an analyte and methods for using the same
AU3260299A (en) Immunoassays involving surface enhanced raman scattering
WO2003066876A3 (en) An apparatus and methods for using biological material to discriminate an agent
CA2266978A1 (en) Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and use for cellular assays in drug discovery
AU2610101A (en) Methods and systems for monitoring intracellular binding reactions
WO2003036256A3 (en) Method and apparatus for increasing the dynamic range and accuracy of binding assays
WO2004025258A3 (en) Gene segregation and biological sample classification methods
CA2330629A1 (en) Method and device for predicting physiological values
EP1318399A3 (en) Passive sample detection to initiate timing of an assay
WO2000023614A8 (en) Method for monitoring proteasome inhibitor drug action
WO2004060044A3 (en) Method and apparatus for molecular analysis in small sample volumes
EP1234053A4 (en) Apparatus and method for assaying coagulation in fluid samples
DK1513878T3 (en) Compositions and Methods for Analysis of Apo B48 and Apo B100
WO2002001230A3 (en) Rapid and sensitive detection of protein aggregation
WO2002093129A3 (en) Methods for analyzing interactions between proteins in live and intact cells
EP0753744A3 (en) Immunophilin-bound immunosuppressant assay
DE60228196D1 (en) SYSTEM AND METHOD FOR THE IN VITRO ANALYSIS OF THERAPEUTIC ACTIVE SUBSTANCES
WO2002003072A8 (en) A method for extracting quantitative information relating to interactions between cellular components
WO2000007017A3 (en) Method of high-throughput screening
WO2003087771A3 (en) Method for making a molecularly smooth surface
WO2001042503A3 (en) Apparatus and methods for drug screening based on nucleic acid analysis
WO2000053625A3 (en) Microarrays of peptide affinity probes for analyzing gene products and methods for analyzing gene products
WO2002079763A3 (en) Tissue-based standoff biosensors for detecting chemical or biological warfare agents
WO2002066982A3 (en) Analysis of modification and demodification of proteins with ubiquitin-related proteins by means of fret (fluorescence resonance energy transfer)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ CZ DE DE DK DK DM EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999948731

Country of ref document: EP

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ CZ DE DE DK DK DM EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 09807345

Country of ref document: US

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 577322

Kind code of ref document: A

Format of ref document f/p: F

WWP Wipo information: published in national office

Ref document number: 1999948731

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWR Wipo information: refused in national office

Ref document number: 1999948731

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999948731

Country of ref document: EP