WO2000017643A3 - A system for cell-based screening - Google Patents

A system for cell-based screening Download PDF

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Publication number
WO2000017643A3
WO2000017643A3 PCT/US1999/021561 US9921561W WO0017643A3 WO 2000017643 A3 WO2000017643 A3 WO 2000017643A3 US 9921561 W US9921561 W US 9921561W WO 0017643 A3 WO0017643 A3 WO 0017643A3
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WO
WIPO (PCT)
Prior art keywords
cells
locations
array
reporter molecules
microplate
Prior art date
Application number
PCT/US1999/021561
Other languages
French (fr)
Other versions
WO2000017643A9 (en
WO2000017643A8 (en
WO2000017643A2 (en
Inventor
R Terry Dunlay
D Lansing Taylor
Albert H Gough
Kenneth A Guiliano
Richard A Rubin
Original Assignee
Cellomics Inc
R Terry Dunlay
D Lansing Taylor
Albert H Gough
Kenneth A Guiliano
Richard A Rubin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc, R Terry Dunlay, D Lansing Taylor, Albert H Gough, Kenneth A Guiliano, Richard A Rubin filed Critical Cellomics Inc
Priority to JP2000571253A priority Critical patent/JP2002525603A/en
Priority to CA002344567A priority patent/CA2344567A1/en
Priority to AU60485/99A priority patent/AU6048599A/en
Priority to EP99969495A priority patent/EP1114320A2/en
Publication of WO2000017643A2 publication Critical patent/WO2000017643A2/en
Publication of WO2000017643A8 publication Critical patent/WO2000017643A8/en
Publication of WO2000017643A9 publication Critical patent/WO2000017643A9/en
Publication of WO2000017643A3 publication Critical patent/WO2000017643A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
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    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
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    • B01J2219/00279—Features relating to reactor vessels
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
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    • B01J2219/00718—Type of compounds synthesised
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    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
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    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
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    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
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    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Abstract

The present invention provides systems, methods, screens, and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having cells in a micropatterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.
PCT/US1999/021561 1998-09-18 1999-09-17 A system for cell-based screening WO2000017643A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000571253A JP2002525603A (en) 1998-09-18 1999-09-17 System for cell-based screening
CA002344567A CA2344567A1 (en) 1998-09-18 1999-09-17 A system for cell-based screening
AU60485/99A AU6048599A (en) 1998-09-18 1999-09-17 A system for cell-based screening
EP99969495A EP1114320A2 (en) 1998-09-18 1999-09-17 A system for cell-based screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10097398P 1998-09-18 1998-09-18
US60/100,973 1998-09-18

Publications (4)

Publication Number Publication Date
WO2000017643A2 WO2000017643A2 (en) 2000-03-30
WO2000017643A8 WO2000017643A8 (en) 2000-06-22
WO2000017643A9 WO2000017643A9 (en) 2000-08-17
WO2000017643A3 true WO2000017643A3 (en) 2000-10-12

Family

ID=22282473

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/021561 WO2000017643A2 (en) 1998-09-18 1999-09-17 A system for cell-based screening

Country Status (5)

Country Link
EP (1) EP1114320A2 (en)
JP (1) JP2002525603A (en)
AU (1) AU6048599A (en)
CA (1) CA2344567A1 (en)
WO (1) WO2000017643A2 (en)

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