WO2000017624A3 - Miniaturized cell array methods and apparatus for cell-based screening - Google Patents

Miniaturized cell array methods and apparatus for cell-based screening Download PDF

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Publication number
WO2000017624A3
WO2000017624A3 PCT/US1999/021729 US9921729W WO0017624A3 WO 2000017624 A3 WO2000017624 A3 WO 2000017624A3 US 9921729 W US9921729 W US 9921729W WO 0017624 A3 WO0017624 A3 WO 0017624A3
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WO
WIPO (PCT)
Prior art keywords
cell
miniaturized
based screening
wells
present
Prior art date
Application number
PCT/US1999/021729
Other languages
French (fr)
Other versions
WO2000017624A2 (en
Inventor
David R Jung
Albert H Gough
D Lansing Taylor
Original Assignee
Cellomics Inc
David R Jung
Albert H Gough
D Lansing Taylor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc, David R Jung, Albert H Gough, D Lansing Taylor filed Critical Cellomics Inc
Priority to JP2000571235A priority Critical patent/JP2002525600A/en
Priority to CA002345151A priority patent/CA2345151A1/en
Priority to EP99969493A priority patent/EP1123499A2/en
Priority to AU60521/99A priority patent/AU752677B2/en
Publication of WO2000017624A2 publication Critical patent/WO2000017624A2/en
Publication of WO2000017624A3 publication Critical patent/WO2000017624A3/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00279—Features relating to reactor vessels
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    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
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    • B01J2219/00686—Automatic
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    • B01J2219/00695—Synthesis control routines, e.g. using computer programs
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00704—Processes involving means for analysing and characterising the products integrated with the reactor apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle

Abstract

The present invention devices and methods for maximizing the number of wells that can be imaged at one time while still obtaining adequate pixel resolution in the image. This result has been achieved through the use of fluidic architectures that maximizes well density. The present invention thus provides a miniaturized microplate system with closed fluidic volumes that are internally supplied with fluid exchange, and with wells that are closely spaced to more rapidly detect spatially-resolved features of individual cells.
PCT/US1999/021729 1998-09-22 1999-09-22 Miniaturized cell array methods and apparatus for cell-based screening WO2000017624A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000571235A JP2002525600A (en) 1998-09-22 1999-09-22 Miniaturized cell array method and device for cell-based screening
CA002345151A CA2345151A1 (en) 1998-09-22 1999-09-22 Miniaturized cell array methods and apparatus for cell-based screening
EP99969493A EP1123499A2 (en) 1998-09-22 1999-09-22 Miniaturized cell array methods and apparatus for cell-based screening
AU60521/99A AU752677B2 (en) 1998-09-22 1999-09-22 Miniaturized cell array methods and apparatus for cell-based screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10139998P 1998-09-22 1998-09-22
US60/101,399 1998-09-22

Publications (2)

Publication Number Publication Date
WO2000017624A2 WO2000017624A2 (en) 2000-03-30
WO2000017624A3 true WO2000017624A3 (en) 2001-01-04

Family

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Application Number Title Priority Date Filing Date
PCT/US1999/021729 WO2000017624A2 (en) 1998-09-22 1999-09-22 Miniaturized cell array methods and apparatus for cell-based screening

Country Status (5)

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EP (1) EP1123499A2 (en)
JP (1) JP2002525600A (en)
AU (1) AU752677B2 (en)
CA (1) CA2345151A1 (en)
WO (1) WO2000017624A2 (en)

Cited By (1)

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