WO2000011024A3 - Novel optical architectures for microvolume laser-scanning cytometers - Google Patents

Novel optical architectures for microvolume laser-scanning cytometers Download PDF

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Publication number
WO2000011024A3
WO2000011024A3 PCT/US1999/019374 US9919374W WO0011024A3 WO 2000011024 A3 WO2000011024 A3 WO 2000011024A3 US 9919374 W US9919374 W US 9919374W WO 0011024 A3 WO0011024 A3 WO 0011024A3
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WO
WIPO (PCT)
Prior art keywords
scanning
laser
sample
bin
confocal
Prior art date
Application number
PCT/US1999/019374
Other languages
French (fr)
Other versions
WO2000011024A2 (en
Inventor
Louis J Dietz
Ian Walton
Scott Norton
Original Assignee
Surromed
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Surromed filed Critical Surromed
Priority to NZ510096A priority Critical patent/NZ510096A/en
Priority to EP99946632A priority patent/EP1121582A4/en
Priority to CA002341359A priority patent/CA2341359A1/en
Priority to AU59001/99A priority patent/AU749690B2/en
Priority to JP2000566296A priority patent/JP2002523731A/en
Priority to KR1020017002397A priority patent/KR20010090718A/en
Publication of WO2000011024A2 publication Critical patent/WO2000011024A2/en
Publication of WO2000011024A3 publication Critical patent/WO2000011024A3/en

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Classifications

    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices

Abstract

Instrumentation and methods for performing charged coupled device (CCD) based confocal spectroscopy with a laser spot array are provided. A sample is scanned by a plurality of excitation laser scanning spots. The scanning spots are generated, for example, by converting a laser excitation beam into an array of beams using a multiple beam generating optic, such as a Damann grating. Each scanning spot scans and excites a separate region of the sample. Fluorescent light emitted by the sample is collected and imaged onto the surface of a CCD detector that has been electronically divided into bins of pixels, with each bin corresponding to one of the scanning spots. Each bin functions as a confocal aperture in which the confocal depth of focus can be controlled by changing the dimensions of the bin. Plural CCD detectors can be used for multichannel imaging of individual wavelengths. The instrumentation and methods are useful in any spectroscopic application including microscopy and microvolume laser scanning cytometry (MLSC).
PCT/US1999/019374 1998-08-21 1999-08-20 Novel optical architectures for microvolume laser-scanning cytometers WO2000011024A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
NZ510096A NZ510096A (en) 1998-08-21 1999-08-20 Confocal scanning system having spot excitation scanner and light detectors
EP99946632A EP1121582A4 (en) 1998-08-21 1999-08-20 Novel optical architectures for microvolume laser-scanning cytometers
CA002341359A CA2341359A1 (en) 1998-08-21 1999-08-20 Novel optical architectures for microvolume laser-scanning cytometers
AU59001/99A AU749690B2 (en) 1998-08-21 1999-08-20 Novel optical architectures for microvolume laser-scanning cytometers
JP2000566296A JP2002523731A (en) 1998-08-21 1999-08-20 Novel optical architecture for microvolume laser scanning cytometry
KR1020017002397A KR20010090718A (en) 1998-08-21 1999-08-20 Novel optical architectures for microvolume laser-scanning cytometers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9750698P 1998-08-21 1998-08-21
US60/097,506 1998-08-21

Publications (2)

Publication Number Publication Date
WO2000011024A2 WO2000011024A2 (en) 2000-03-02
WO2000011024A3 true WO2000011024A3 (en) 2000-06-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/019374 WO2000011024A2 (en) 1998-08-21 1999-08-20 Novel optical architectures for microvolume laser-scanning cytometers

Country Status (9)

Country Link
US (3) US6603537B1 (en)
EP (1) EP1121582A4 (en)
JP (1) JP2002523731A (en)
KR (1) KR20010090718A (en)
AU (1) AU749690B2 (en)
CA (1) CA2341359A1 (en)
NZ (1) NZ510096A (en)
WO (1) WO2000011024A2 (en)
ZA (1) ZA200101459B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9297766B2 (en) 2001-01-26 2016-03-29 Becton, Dickinson And Company Method of tagging materials with surface-enhanced spectroscopy-active sandwich particles

Families Citing this family (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19829981C2 (en) * 1998-07-04 2002-10-17 Zeiss Carl Jena Gmbh Method and arrangement for confocal microscopy
EP1121582A4 (en) * 1998-08-21 2002-10-23 Surromed Inc Novel optical architectures for microvolume laser-scanning cytometers
US7387891B2 (en) * 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
US20050279949A1 (en) * 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7410793B2 (en) * 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US8264680B2 (en) * 1999-05-28 2012-09-11 Yokogawa Electric Corporation Biochip reader and electrophoresis system
EP1983331B1 (en) * 1999-05-28 2011-07-13 Yokogawa Electric Corporation Optical system for reading a biochip
US20110141299A1 (en) * 1999-06-30 2011-06-16 Masten Opto-Diagnostics Company Low cost combination spectral-spatial vision system
US6687395B1 (en) * 1999-07-21 2004-02-03 Surromed, Inc. System for microvolume laser scanning cytometry
US6403332B1 (en) * 1999-07-30 2002-06-11 California Institute Of Technology System and method for monitoring cellular activity
US7217573B1 (en) * 1999-10-05 2007-05-15 Hitachi, Ltd. Method of inspecting a DNA chip
US7192778B2 (en) 1999-10-06 2007-03-20 Natan Michael J Surface enhanced spectroscopy-active composite nanoparticles
US8497131B2 (en) 1999-10-06 2013-07-30 Becton, Dickinson And Company Surface enhanced spectroscopy-active composite nanoparticles comprising Raman-active reporter molecules
JP2001166214A (en) * 1999-12-03 2001-06-22 Olympus Optical Co Ltd Optical device
US6690467B1 (en) * 2000-05-05 2004-02-10 Pe Corporation Optical system and method for optically analyzing light from a sample
EP1953580B1 (en) * 2000-09-18 2014-09-17 Vincent Lauer Confocal optical scanning device
JP4426080B2 (en) * 2000-10-11 2010-03-03 株式会社メニコン Method and apparatus for detecting dirt on ophthalmic lens
US6787761B2 (en) * 2000-11-27 2004-09-07 Surromed, Inc. Median filter for liquid chromatography-mass spectrometry data
US6615063B1 (en) * 2000-11-27 2003-09-02 The General Hospital Corporation Fluorescence-mediated molecular tomography
US7383076B2 (en) 2000-11-27 2008-06-03 The General Hospital Corporation Fluorescence-mediated molecular tomography
US20020095260A1 (en) * 2000-11-28 2002-07-18 Surromed, Inc. Methods for efficiently mining broad data sets for biological markers
JP2002236257A (en) * 2001-02-13 2002-08-23 Dainippon Printing Co Ltd Multicolor confocal microscope
EP1377853B1 (en) 2001-04-10 2008-09-17 President And Fellows of Harvard College Microlens for projection lithography and method of preparation thereof
DE10136863A1 (en) * 2001-07-28 2003-02-20 Berthold Tech Gmbh & Co Kg Device for the optional measurement of in particular luminescence and / or fluorescence radiation
US6873915B2 (en) * 2001-08-24 2005-03-29 Surromed, Inc. Peak selection in multidimensional data
US20030078739A1 (en) * 2001-10-05 2003-04-24 Surromed, Inc. Feature list extraction from data sets such as spectra
DE10214517B4 (en) * 2001-12-03 2013-06-13 Sensovation Ag Apparatus and method for photoelectric measurement
US7158294B2 (en) * 2001-12-18 2007-01-02 Olympus Optical Co., Ltd. Laser scanning confocal microscope apparatus, image recording method, and recording medium
JP2005515642A (en) * 2002-01-19 2005-05-26 スフェロン ヴィアール アクチエンゲゼルシャフト Apparatus and method for distance measurement
WO2003095978A2 (en) * 2002-05-09 2003-11-20 Surromed, Inc. Methods for time-alignment of liquid chromatography-mass spectrometry data
JP2005526253A (en) 2002-05-17 2005-09-02 アプレラ コーポレイション Apparatus and method for differentiating multiple fluorescent signals by excitation wavelength
US6768122B2 (en) * 2002-06-04 2004-07-27 National Taiwan University Multiphoton excitation microscope for biochip fluorescence assay
GB0216197D0 (en) * 2002-07-12 2002-08-21 Univ Strathclyde Serrs active particles
US11243494B2 (en) 2002-07-31 2022-02-08 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US20040120577A1 (en) * 2002-10-06 2004-06-24 Igor Touzov Digital video based array detector for parallel process control and radiation driver for micro-scale devices
DK1411345T3 (en) * 2002-10-17 2007-01-02 Direvo Biotech Ag Multiparameter fluorimetric analysis in parallel with multifocal arrangement
US7170598B2 (en) * 2002-10-17 2007-01-30 Direvo Biotech Ag Multi-parameter fluorimetric analysis in a massively parallel multi-focal arrangement and the use thereof
EP1558934B1 (en) * 2002-10-31 2013-07-17 ChemoMetec A/S A method for assessment of particles
US7053783B2 (en) 2002-12-18 2006-05-30 Biovigilant Systems, Inc. Pathogen detector system and method
US20060038979A1 (en) * 2004-08-05 2006-02-23 Michael Natan Nanoparticles as covert taggants in currency, bank notes, and related documents
EP1627076A4 (en) * 2003-03-14 2008-07-02 Ppd Biomarker Discovery Scienc Biological markers for diagnosing rheumatoid arthritis
US7633616B2 (en) 2003-06-02 2009-12-15 Sensovation Ag Apparatus and method for photo-electric measurement
WO2005019811A2 (en) * 2003-08-26 2005-03-03 Blueshift Biotechnologies, Inc. Time dependent fluorescence measurements
US7298474B2 (en) * 2003-10-24 2007-11-20 Purdue Research Foundation Plasmonic and/or microcavity enhanced optical protein sensing
KR100619365B1 (en) 2003-11-03 2006-09-12 삼성전기주식회사 Scanning apparatus using diffraction multi-beam
US6964530B2 (en) * 2004-01-23 2005-11-15 Hewlett-Packard Development Company, L.P. Self-correcting printing system
US7248360B2 (en) 2004-04-02 2007-07-24 Ppd Biomarker Discovery Sciences, Llc Polychronic laser scanning system and method of use
JP2006023221A (en) * 2004-07-09 2006-01-26 Tokyo Seimitsu Co Ltd Visual inspection device and projection method
US7697808B2 (en) * 2004-07-27 2010-04-13 Ut-Battelle, Llc Multi-tipped optical component
WO2006073492A2 (en) 2004-07-30 2006-07-13 Biovigilant Systems, Inc. Pathogen and particle detector system and method
US20080151238A1 (en) * 2005-03-01 2008-06-26 Cornell Research Foundation, Inc. Simultaneous Spatial and Temporal Focusing of Femtosecond Pulses
WO2006127967A2 (en) 2005-05-25 2006-11-30 Massachusetts Institute Of Technology Multifocal scanning microscopy systems and methods
TWI295380B (en) * 2005-05-26 2008-04-01 Cymer Inc Systems and methods for implementing an interaction between a laser shaped as a line beam and a film deposited on a substrate
US9410889B2 (en) 2005-06-10 2016-08-09 Applied Biosystem, Llc Method and system for multiplex genetic analysis
CN101223560B (en) * 2005-07-15 2012-12-12 百维吉伦特系统有限公司 Pathogen and particle detector system and method
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US7996188B2 (en) 2005-08-22 2011-08-09 Accuri Cytometers, Inc. User interface for a flow cytometer system
US7397042B2 (en) 2005-08-24 2008-07-08 Dr. Chip Biotechnology Incorporation Optical detection apparatus and method thereof
US8303894B2 (en) 2005-10-13 2012-11-06 Accuri Cytometers, Inc. Detection and fluidic system of a flow cytometer
US8017402B2 (en) 2006-03-08 2011-09-13 Accuri Cytometers, Inc. Fluidic system for a flow cytometer
US7329860B2 (en) * 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
US8409863B2 (en) 2005-12-14 2013-04-02 Becton, Dickinson And Company Nanoparticulate chemical sensors using SERS
US7723100B2 (en) 2006-01-13 2010-05-25 Becton, Dickinson And Company Polymer coated SERS nanotag
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) * 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US8031340B2 (en) * 2006-02-22 2011-10-04 Accuri Cytometers, Inc. Optical system for a flow cytometer
US8149402B2 (en) * 2006-02-22 2012-04-03 Accuri Cytometers, Inc. Optical system for a flow cytometer
EP1987389B1 (en) * 2006-02-22 2017-06-14 Accuri Instruments Inc. Optical system for a flow cytometer
US8283177B2 (en) 2006-03-08 2012-10-09 Accuri Cytometers, Inc. Fluidic system with washing capabilities for a flow cytometer
US7780916B2 (en) 2006-03-08 2010-08-24 Accuri Cytometers, Inc. Flow cytometer system with unclogging feature
WO2008028160A2 (en) * 2006-09-01 2008-03-06 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
US8715573B2 (en) 2006-10-13 2014-05-06 Accuri Cytometers, Inc. Fluidic system for a flow cytometer with temporal processing
US8445286B2 (en) * 2006-11-07 2013-05-21 Accuri Cytometers, Inc. Flow cell for a flow cytometer system
JP2008139280A (en) * 2006-11-09 2008-06-19 Toyohashi Univ Of Technology Method and apparatus using evanescent light for measuring concentration distribution of chemical substance
US8345241B1 (en) * 2006-12-19 2013-01-01 J. A. Woollam Co., Inc. Application of digital light processor in imaging ellipsometer and the like systems
US7739060B2 (en) * 2006-12-22 2010-06-15 Accuri Cytometers, Inc. Detection system and user interface for a flow cytometer system
US7804594B2 (en) * 2006-12-29 2010-09-28 Abbott Laboratories, Inc. Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
ITMI20071632A1 (en) * 2007-08-06 2009-02-07 Consiglio Nazionale Ricerche APPARATUS FOR AFTERFLOWING INVESTIGATIONS AND METHODS OF OPTIMIZATION OF THE CONDITIONING CHAIN
ITMI20071631A1 (en) * 2007-08-06 2009-02-07 Consiglio Nazionale Ricerche APPARATUS FOR CITOFLUORIMETRIC INVESTIGATIONS
EP3067729B1 (en) * 2007-09-03 2020-09-23 Nikon Corporation Automatic focusing device and microscope
CN104765041B (en) * 2007-10-09 2019-07-26 文达光电股份有限公司 Coherent lidar system based on semiconductor lasers and amplifiers
JP4525725B2 (en) * 2007-10-18 2010-08-18 ソニー株式会社 OPTICAL MEASUREMENT UNIT, OPTICAL MEASUREMENT MEMBER, FINE PARTICLE MEASUREMENT DEVICE PROVIDED WITH THE SAME, AND MICROPARTICLE OPTICAL MEASUREMENT METHOD
JP4509166B2 (en) 2007-11-02 2010-07-21 ソニー株式会社 Method and apparatus for measuring fine particles
US8159670B2 (en) 2007-11-05 2012-04-17 Abbott Laboratories Method and apparatus for rapidly counting and identifying biological particles in a flow stream
US8628976B2 (en) * 2007-12-03 2014-01-14 Azbil BioVigilant, Inc. Method for the detection of biologic particle contamination
US7843561B2 (en) 2007-12-17 2010-11-30 Accuri Cytometers, Inc. Optical system for a flow cytometer with an interrogation zone
US8432541B2 (en) 2007-12-17 2013-04-30 Accuri Cytometers, Inc. Optical system for a flow cytometer with an interrogation zone
DE102008012635A1 (en) 2008-03-05 2009-09-10 Carl Zeiss Microlmaging Gmbh Method and arrangement for time-resolved spectroscopy
EP2331934B1 (en) 2008-09-16 2020-01-01 Pacific Biosciences of California, Inc. Analytic device including a zero mode waveguide substrate
US8928881B2 (en) 2009-01-23 2015-01-06 University Of Washington Cytometer with automatic continuous alignment correction
US8284402B2 (en) * 2009-02-27 2012-10-09 Beckman Coulter, Inc. Fluorescence detection instrument with orthogonal laser entry
US8507279B2 (en) 2009-06-02 2013-08-13 Accuri Cytometers, Inc. System and method of verification of a prepared sample for a flow cytometer
KR20120018184A (en) * 2009-06-02 2012-02-29 씨8 메디센서스, 인크. All reflective apparatus for injecting excitation light and collecting in-elastically scattered light from a sample
EP2537053B1 (en) 2010-02-19 2016-11-02 Pacific Biosciences of California, Inc. An analytical device comprising an optode array chip
US8994946B2 (en) 2010-02-19 2015-03-31 Pacific Biosciences Of California, Inc. Integrated analytical system and method
WO2011106402A1 (en) 2010-02-23 2011-09-01 Accuri Cytometers, Inc. Method and system for detecting fluorochromes in a flow cytometer
US9551600B2 (en) 2010-06-14 2017-01-24 Accuri Cytometers, Inc. System and method for creating a flow cytometer network
EP2633284B1 (en) 2010-10-25 2021-08-25 Accuri Cytometers, Inc. Systems and user interface for collecting a data set in a flow cytometer
WO2012059784A1 (en) 2010-11-03 2012-05-10 Reametrix Inc Method and device for fluorescent measurement of samples
US10908066B2 (en) 2010-11-16 2021-02-02 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
JP5838466B2 (en) * 2011-03-11 2016-01-06 ナノフォトン株式会社 Optical microscope and spectroscopic measurement method
US20120257037A1 (en) * 2011-04-07 2012-10-11 Valerica Raicu High speed microscope with two-stage scanning for detection of rarities in samples
US9137463B2 (en) * 2011-05-12 2015-09-15 Microsoft Technology Licensing, Llc Adaptive high dynamic range camera
US8837045B2 (en) * 2012-09-21 2014-09-16 The Board Of Trustees Of The University Of Illinois Diffraction phase microscopy with white light
US9372308B1 (en) 2012-06-17 2016-06-21 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices and methods for production
EP3734255B1 (en) 2012-12-18 2022-10-19 Pacific Biosciences Of California, Inc. An optical analytical device
US8873041B1 (en) * 2013-01-29 2014-10-28 Bayspec, Inc. Raman spectroscopy using multiple excitation wavelengths
EP2959283B1 (en) 2013-02-22 2022-08-17 Pacific Biosciences of California, Inc. Integrated illumination of optical analytical devices
US9372143B2 (en) 2013-05-15 2016-06-21 Captl Llc Scanning image flow cytometer
US9562860B1 (en) 2013-06-19 2017-02-07 Theranos, Inc. Methods and devices for sample analysis
US8961904B2 (en) 2013-07-16 2015-02-24 Premium Genetics (Uk) Ltd. Microfluidic chip
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
WO2015100421A1 (en) * 2013-12-24 2015-07-02 Tissuevision, Inc. Multi-foci multiphoton imaging systems and methods
JP6284372B2 (en) * 2014-01-21 2018-02-28 オリンパス株式会社 Scanning laser microscope and super-resolution image generation method
US9828284B2 (en) 2014-03-28 2017-11-28 Ut-Battelle, Llc Thermal history-based etching
TWI692633B (en) 2014-08-27 2020-05-01 美商加州太平洋生物科學公司 Arrays of integrated analytical devices
WO2016100954A1 (en) 2014-12-19 2016-06-23 Captl Llc Flow cytometry using hydrodynamically planar flow
WO2016099538A1 (en) 2014-12-19 2016-06-23 Captl Llc Flow cytometry using hydrodynamically planar flow
SG11201706777QA (en) 2015-02-19 2017-09-28 Premium Genetics (Uk) Ltd Scanning infrared measurement system
US10788403B2 (en) 2015-03-11 2020-09-29 Tissuevision, Inc. Systems and methods for serial staining and imaging
WO2016149397A1 (en) 2015-03-16 2016-09-22 Pacific Biosciences Of California, Inc. Integrated devices and systems for free-space optical coupling
AU2016276980B2 (en) 2015-06-12 2021-09-23 Pacific Biosciences Of California, Inc. Integrated target waveguide devices and systems for optical coupling
US10036698B2 (en) 2015-06-19 2018-07-31 Captl Llc Time-sequential cytometry
WO2017131759A1 (en) 2016-01-29 2017-08-03 Hewlett-Packard Development Company, L.P. Optical readers
DE102016119727A1 (en) * 2016-10-17 2018-04-19 Carl Zeiss Microscopy Gmbh Device for beam manipulation for a scanning microscope and microscope
US11473081B2 (en) 2016-12-12 2022-10-18 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
CN106547079B (en) * 2017-01-17 2018-11-20 中国科学院上海光学精密机械研究所 Real-time three-dimensional laser fluorescence microscopic imaging device
CN107402197A (en) * 2017-02-23 2017-11-28 武汉能斯特科技有限公司 The scanning detection method and equipment of a kind of delayed luminescence
CA3067870A1 (en) * 2017-06-22 2018-12-27 Arthur Edward Dixon Msia scanning instrument with increased dynamic range
WO2019012776A1 (en) * 2017-07-11 2019-01-17 浜松ホトニクス株式会社 Sample observation device and sample observation method
CN110118725B (en) * 2018-02-07 2021-08-31 清华大学 Photocurrent scanning system
US10989903B2 (en) * 2018-02-26 2021-04-27 Washington State University Modular scanning confocal optical profile microscopy with digital imaging processing
EP3796998A1 (en) 2018-05-23 2021-03-31 ABS Global, Inc. Systems and methods for particle focusing in microchannels
US10866092B2 (en) * 2018-07-24 2020-12-15 Kla-Tencor Corporation Chromatic confocal area sensor
GB2592141B (en) * 2018-10-12 2022-10-12 Hitachi High Tech Corp Dichroic mirror array and light detecting device
US10760969B1 (en) 2019-02-28 2020-09-01 Biospex, Inc. Fluorescence and systemic noise reduction in time-gated spectroscopy
JP2022537617A (en) * 2019-04-09 2022-08-29 カール ツァイス マイクロスコピー ゲーエムベーハー Optical microscope with reconfigurable sensor array
WO2020215011A1 (en) 2019-04-18 2020-10-22 Abs Global, Inc. System and process for continuous addition of cryoprotectant
US11454540B2 (en) * 2019-07-12 2022-09-27 Biospex, Inc. Wearable spectroscopy using filtered sensor
US11326944B2 (en) * 2019-07-12 2022-05-10 Biospex, Inc. Wearable spectrometer with filtered sensor
IL292781A (en) * 2019-11-08 2022-07-01 Xcella Biosciences Inc Apparatus and methods for laser-based single cell recovery from microcapillary arrays
US11628439B2 (en) 2020-01-13 2023-04-18 Abs Global, Inc. Single-sheath microfluidic chip
CN113698093B (en) * 2021-07-29 2023-03-10 杭州富通通信技术股份有限公司 Optical fiber processing technology
CN113624666B (en) * 2021-09-07 2022-09-23 清华大学 Stream type imaging system based on dot matrix laser scanning
KR102645654B1 (en) * 2021-12-22 2024-03-13 (주)얼라인드제네틱스 A line confocal cell counter for high throughput multispectral detection of cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5446532A (en) * 1992-06-09 1995-08-29 Canon Kabushiki Kaisha Measuring apparatus with optically conjugate radiation fulcrum and irradiated area
US5726751A (en) * 1995-09-27 1998-03-10 University Of Washington Silicon microchannel optical flow cytometer

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE458968B (en) 1987-06-16 1989-05-22 Wallac Oy BIOSPECIFIC ANALYTICAL PROCEDURE FOR MULTIPLE ANALYTICS WHICH DO NOT INCLUDE PARTICULAR COATING AND LABELING WITH FLUORESCING LABEL SUBSTANCES
US5091652A (en) * 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
GB9014263D0 (en) * 1990-06-27 1990-08-15 Dixon Arthur E Apparatus and method for spatially- and spectrally- resolvedmeasurements
GB9015793D0 (en) * 1990-07-18 1990-09-05 Medical Res Council Confocal scanning optical microscope
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
US5239178A (en) * 1990-11-10 1993-08-24 Carl Zeiss Optical device with an illuminating grid and detector grid arranged confocally to an object
US5736410A (en) * 1992-09-14 1998-04-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5556764A (en) 1993-02-17 1996-09-17 Biometric Imaging, Inc. Method and apparatus for cell counting and cell classification
US5547849A (en) 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
FI96452C (en) * 1994-01-26 1996-06-25 Pekka Haenninen Method for excitation of dyes
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5682038A (en) * 1995-04-06 1997-10-28 Becton Dickinson And Company Fluorescent-particle analyzer with timing alignment for analog pulse subtraction of fluorescent pulses arising from different excitation locations
US6017434A (en) * 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6104945A (en) * 1995-08-01 2000-08-15 Medispectra, Inc. Spectral volume microprobe arrays
US5814820A (en) * 1996-02-09 1998-09-29 The Board Of Trustees Of The University Of Illinois Pump probe cross correlation fluorescence frequency domain microscope and microscopy
DE19707227A1 (en) 1997-02-24 1998-08-27 Bodenseewerk Perkin Elmer Co Light scanner
US6388788B1 (en) * 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
EP1121582A4 (en) 1998-08-21 2002-10-23 Surromed Inc Novel optical architectures for microvolume laser-scanning cytometers
US6377842B1 (en) * 1998-09-22 2002-04-23 Aurora Optics, Inc. Method for quantitative measurement of fluorescent and phosphorescent drugs within tissue utilizing a fiber optic probe
US6376843B1 (en) * 1999-06-23 2002-04-23 Evotec Oai Ag Method of characterizing fluorescent molecules or other particles using generating functions
JP3663125B2 (en) * 2000-11-28 2005-06-22 日立ソフトウエアエンジニアリング株式会社 Fluorescence reading method and fluorescence reading apparatus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5446532A (en) * 1992-06-09 1995-08-29 Canon Kabushiki Kaisha Measuring apparatus with optically conjugate radiation fulcrum and irradiated area
US5726751A (en) * 1995-09-27 1998-03-10 University Of Washington Silicon microchannel optical flow cytometer

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9297766B2 (en) 2001-01-26 2016-03-29 Becton, Dickinson And Company Method of tagging materials with surface-enhanced spectroscopy-active sandwich particles

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US20040079893A1 (en) 2004-04-29
US6800860B2 (en) 2004-10-05
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US6603537B1 (en) 2003-08-05
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AU5900199A (en) 2000-03-14
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