WO2000008131A3 - Pseudotyped retroviruses and stable cell lines for their production - Google Patents

Pseudotyped retroviruses and stable cell lines for their production Download PDF

Info

Publication number
WO2000008131A3
WO2000008131A3 PCT/US1999/017702 US9917702W WO0008131A3 WO 2000008131 A3 WO2000008131 A3 WO 2000008131A3 US 9917702 W US9917702 W US 9917702W WO 0008131 A3 WO0008131 A3 WO 0008131A3
Authority
WO
WIPO (PCT)
Prior art keywords
retroviruses
pseudotyped
cells
glycoproteins
methods
Prior art date
Application number
PCT/US1999/017702
Other languages
French (fr)
Other versions
WO2000008131A9 (en
WO2000008131A2 (en
WO2000008131A8 (en
Inventor
David A Sanders
Richard John Kuhn
Scott A Jeffers
Curtis Matthew Sharkey
Cynthia Lin North
Michael A Fischbach
Original Assignee
Purdue Research Foundation
David A Sanders
Richard John Kuhn
Scott A Jeffers
Curtis Matthew Sharkey
Cynthia Lin North
Michael A Fischbach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, David A Sanders, Richard John Kuhn, Scott A Jeffers, Curtis Matthew Sharkey, Cynthia Lin North, Michael A Fischbach filed Critical Purdue Research Foundation
Priority to US09/762,224 priority Critical patent/US7033595B1/en
Priority to AU54658/99A priority patent/AU5465899A/en
Publication of WO2000008131A2 publication Critical patent/WO2000008131A2/en
Publication of WO2000008131A3 publication Critical patent/WO2000008131A3/en
Publication of WO2000008131A9 publication Critical patent/WO2000008131A9/en
Publication of WO2000008131A8 publication Critical patent/WO2000008131A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13061—Methods of inactivation or attenuation
    • C12N2740/13062—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/14011—Filoviridae
    • C12N2760/14111—Ebolavirus, e.g. Zaire ebolavirus
    • C12N2760/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

Cells that produce inventive pseudotyped retroviruses having a broad host range have been produced. In one aspect of the invention, the cells produce retroviruses pseudotyped with at least two different viral glycoproteins, such as togaviral glycoproteins. In alternative embodiments, the cells produce retroviruses pseudotyped with filoviral glycoproteins. Methods of producing the above-described cells, as well as the pseudotyped retroviruses thus produced, are also provided. In other embodiments, methods of screening agents effective in blocking viral entry into a cell, including filoviral entry or entry of viruses having at least two different viral glycoproteins disposed in their lipid bilayer, such as togaviruses, are provided. Moreover, methods of using the inventive pseudotyped retroviruses for introducing nucleotide sequences into target cells, and kits for forming the inventive pseudotyped retroviruses, are also provided.
PCT/US1999/017702 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production WO2000008131A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/762,224 US7033595B1 (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production
AU54658/99A AU5465899A (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9524298P 1998-08-04 1998-08-04
US60/095,242 1998-08-04
US11240598P 1998-12-15 1998-12-15
US60/112,405 1998-12-15

Publications (4)

Publication Number Publication Date
WO2000008131A2 WO2000008131A2 (en) 2000-02-17
WO2000008131A3 true WO2000008131A3 (en) 2000-06-02
WO2000008131A9 WO2000008131A9 (en) 2000-08-03
WO2000008131A8 WO2000008131A8 (en) 2001-01-04

Family

ID=26789991

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/017702 WO2000008131A2 (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production

Country Status (2)

Country Link
AU (1) AU5465899A (en)
WO (1) WO2000008131A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001083730A2 (en) * 2000-04-28 2001-11-08 The Trustees Of The University Of Pennsylvania Recombinant lentiviral vectors pseudotyped in envelopes containing filovirus binding domains
WO2003035849A2 (en) * 2001-10-26 2003-05-01 Purdue Research Foundation Methods for gene transfer using pseudotyped lentiviruses
US7981656B2 (en) 2002-06-04 2011-07-19 Purdue Research Foundation Pseudotyped retrovirus with modified ebola glycoprotein
CA2960096A1 (en) * 2014-09-03 2016-03-10 Bavarian Nordic A/S Methods and compositions for enhancing immune responses
BR112017003891A2 (en) * 2014-09-03 2017-12-26 Bavarian Nordic As methods and compositions for inducing protective immunity against filovirus infection
WO2018011768A1 (en) 2016-07-15 2018-01-18 Janssen Vaccines And Prevention B.V. Methods and compositions for inducing protective immunity against a marburg virus infection
WO2018185732A1 (en) 2017-04-06 2018-10-11 Janssen Vaccines & Prevention B.V. Mva-bn and ad26.zebov or ad26.filo prime-boost regimen

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4912030A (en) * 1985-01-15 1990-03-27 Institute Of Cancer Research Viral isolates and their use in diagnosis
US5185440A (en) * 1989-06-20 1993-02-09 North Carolina State University cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5503974A (en) * 1989-06-30 1996-04-02 University Of California Retrovirus detection
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
US5723287A (en) * 1992-09-22 1998-03-03 Medical Research Council Recombinant viruses displaying a nonviral polypeptide on their external surface
US5739018A (en) * 1996-08-07 1998-04-14 The Regents Of The University Of California Packaging cell lines for pseudotyped retroviral vectors

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4912030A (en) * 1985-01-15 1990-03-27 Institute Of Cancer Research Viral isolates and their use in diagnosis
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
US5185440A (en) * 1989-06-20 1993-02-09 North Carolina State University cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof
US5503974A (en) * 1989-06-30 1996-04-02 University Of California Retrovirus detection
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
US5723287A (en) * 1992-09-22 1998-03-03 Medical Research Council Recombinant viruses displaying a nonviral polypeptide on their external surface
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5739018A (en) * 1996-08-07 1998-04-14 The Regents Of The University Of California Packaging cell lines for pseudotyped retroviral vectors

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KUHN R.J. ET AL.: "Chimeric Sindbis-Ross River Viruses to Study Interactions between Alphavirus Nonstructural and Structural Regions", J. VIROL.,, vol. 70, no. 11, November 1996 (1996-11-01), pages 7900 - 7909, XP002930976 *
LOPEZ S. ET AL.: "Nucleocapsid-Glycoprotein Interactions Required for Assembly of Alphaviruses", J. VIROL.,, vol. 68, no. 3, March 1994 (1994-03-01), pages 1316 - 1323, XP002930975 *
SANCHEZ ET AL.: "Sequence analysis of the Ebola virus genome: Organization, genetic elements and comparison with the genome of Marburg virus", VIRUS RESEARCH,, vol. 29, 1993, pages 215 - 240, XP002931007 *
WILL ET AL.: "Marburg virus gene 4 encodes the virion membrane protein, a type I transmembrane glycoprotein", J. VIROL.,, vol. 67, no. 3, March 1993 (1993-03-01), pages 1203 - 1210, XP002931008 *

Also Published As

Publication number Publication date
AU5465899A (en) 2000-02-28
WO2000008131A9 (en) 2000-08-03
WO2000008131A2 (en) 2000-02-17
WO2000008131A8 (en) 2001-01-04

Similar Documents

Publication Publication Date Title
AU4712397A (en) Lentiviral vectors
AU2002216157A1 (en) Composite beam with integrated break-off initiator and aircraft fuselage incorporating such beams
BR9809155A (en) Useful structures like sheets for cleaning
DK0751129T3 (en) Substituted dihydrodibenz / b, f / azepines, process for their preparation, their use in the treatment of some
WO2000040525A3 (en) Polymer arrays and methods of making and using same
AU7808198A (en) Novel compounds useful in the complement, coagulat and kallikrein pathways and method for their preparation
AU1843501A (en) A building structure utilising modular building elements
WO2000008131A3 (en) Pseudotyped retroviruses and stable cell lines for their production
AU7521698A (en) Method for producing paper, pulpboard and cardboard
AU5397696A (en) Carbohydrate-modified cytostatics
EP1163515A4 (en) Therapeutic and diagnostic tools for impaired glucose tolerance conditions
AU2711099A (en) Element comprised of a plurality of strips running in a horizontal and vertical manner, and a method for producing elements by connecting closed rings
AU1058999A (en) A stud for a timber wall
TR200002140T2 (en) New sulfonamides replaced with naphthyl and anilide
ATE323776T1 (en) RETROVIRAL VECTORS FOR EXPRESSION IN EMBRYONAL CELLS
WO2001045186A3 (en) Substrate-supported electrode-electrolyte unit
FR2701335B1 (en) Differential protection block with testable functional sub-assembly.
WO2000028945A3 (en) Anticonvulsant derivatives useful in treating post traumatic stress disorder
CA2349371A1 (en) Chip and method for the production of wood pulp
WO2003102219A3 (en) Improved pseudotyped retroviruses
EP0899375A3 (en) High consistency damless refiner plate for wood fibers
FR2788942B1 (en) RIGID STRUCTURE, IN PARTICULAR A STRUCTURE FORMING AN INDUSTRIAL COOKING TOOL.
CA2359907A1 (en) Textile
AUPQ616300A0 (en) A toy
AU1491499A (en) Cloning method by multiple digestion, vectors for implementing same and applications

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: C2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/4-4/4, DRAWINGS, REPLACED BY NEW PAGES 1/6-6/6; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: C1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WR Later publication of a revised version of an international search report
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 09762224

Country of ref document: US

122 Ep: pct application non-entry in european phase
REF Corresponds to

Ref document number: 10192921

Country of ref document: DE

Date of ref document: 20021121

Format of ref document f/p: P