WO1999060408A3 - Assay involving protein binding to 3' untranslated mrna sequences - Google Patents
Assay involving protein binding to 3' untranslated mrna sequences Download PDFInfo
- Publication number
- WO1999060408A3 WO1999060408A3 PCT/US1999/010531 US9910531W WO9960408A3 WO 1999060408 A3 WO1999060408 A3 WO 1999060408A3 US 9910531 W US9910531 W US 9910531W WO 9960408 A3 WO9960408 A3 WO 9960408A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protein binding
- mrna sequences
- protein
- assay involving
- involving protein
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU39020/99A AU3902099A (en) | 1998-05-20 | 1999-05-12 | Tethered function assay for protein function |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/081,731 | 1998-05-20 | ||
US09/081,731 US5985575A (en) | 1998-05-20 | 1998-05-20 | Tethered function assay for protein function |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1999060408A2 WO1999060408A2 (en) | 1999-11-25 |
WO1999060408A3 true WO1999060408A3 (en) | 2000-01-20 |
Family
ID=22166026
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1999/010531 WO1999060408A2 (en) | 1998-05-20 | 1999-05-12 | Assay involving protein binding to 3' untranslated mrna sequences |
Country Status (3)
Country | Link |
---|---|
US (2) | US5985575A (en) |
AU (1) | AU3902099A (en) |
WO (1) | WO1999060408A2 (en) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6261804B1 (en) * | 1997-01-21 | 2001-07-17 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
US8207093B2 (en) * | 1997-01-21 | 2012-06-26 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
KR100566859B1 (en) | 1997-01-21 | 2006-04-03 | 제너럴 하스피톨 코포레이션 | Selection of proteins using rna-protein fusions |
IL138668A0 (en) * | 1998-04-03 | 2001-10-31 | Phylos Inc | Addressable protein arrays |
JP4278872B2 (en) | 1998-08-17 | 2009-06-17 | フィロス インク. | Identification of compound-protein interaction using protein-nucleic acid fusion molecule library |
CA2334946A1 (en) * | 1998-08-17 | 2000-02-24 | Phylos, Inc. | Methods for producing nucleic acids lacking 3'-untranslated regions and optimizing cellular rna-protein fusion formation |
ATE354675T1 (en) * | 1998-12-02 | 2007-03-15 | Adnexus Therapeutics Inc | DNA-PROTEIN FUSIONS AND APPLICATIONS THEREOF |
US6623926B1 (en) | 1999-06-01 | 2003-09-23 | Phylos, Inc. | Methods for producing 5′-nucleic acid-protein conjugates |
US6429300B1 (en) * | 1999-07-27 | 2002-08-06 | Phylos, Inc. | Peptide acceptor ligation methods |
WO2001053539A1 (en) | 2000-01-24 | 2001-07-26 | Phylos, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
US7022479B2 (en) * | 2000-01-24 | 2006-04-04 | Compound Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
IL134830A0 (en) * | 2000-03-01 | 2001-05-20 | Chay 13 Medical Res Group N V | Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them |
WO2001084155A1 (en) * | 2000-04-28 | 2001-11-08 | Message Pharmaceuticals, Inc. | Methods for identifying novel nucleic acid regulatory elements and compounds that affect the regulation |
US20030198973A1 (en) * | 2002-04-23 | 2003-10-23 | The Molecular Sciences Institute, Inc. | Chimeric fusion molecule for analyte detection and quantitation |
US20060073481A1 (en) * | 2002-04-23 | 2006-04-06 | The Molecular Sciences Institute | Formation and use of site specific nucleic acid coupled binding polypeptides |
US20040235001A1 (en) * | 2002-09-18 | 2004-11-25 | Liaoteng Wang | Regulatory poly(A) polymerase and uses thereof |
US7919583B2 (en) * | 2005-08-08 | 2011-04-05 | Discovery Genomics, Inc. | Integration-site directed vector systems |
JP2009008608A (en) * | 2007-06-29 | 2009-01-15 | Sysmex Corp | Method and kit for determining amount of deoxyribonucleic acid (dna)-binding protein |
CN108690139B (en) | 2017-07-31 | 2019-03-15 | 康码(上海)生物科技有限公司 | The preparation of new fusion protein and its application synthesized in raising protein |
EP3762499B1 (en) | 2018-03-06 | 2023-12-20 | Danisco Us Inc | Reduction in acetate production by yeast over-expressing pab1 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998037422A1 (en) * | 1997-02-20 | 1998-08-27 | Message Pharmaceuticals, Inc. | Method for identifying binding sites for rna binding proteins |
US5885779A (en) * | 1997-09-09 | 1999-03-23 | University Of British Columbia | Repressed trans-activator system for characterization of protein-protein interactions |
-
1998
- 1998-05-20 US US09/081,731 patent/US5985575A/en not_active Expired - Fee Related
-
1999
- 1999-05-12 WO PCT/US1999/010531 patent/WO1999060408A2/en active Application Filing
- 1999-05-12 AU AU39020/99A patent/AU3902099A/en not_active Abandoned
- 1999-07-27 US US09/361,727 patent/US6303311B1/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998037422A1 (en) * | 1997-02-20 | 1998-08-27 | Message Pharmaceuticals, Inc. | Method for identifying binding sites for rna binding proteins |
US5885779A (en) * | 1997-09-09 | 1999-03-23 | University Of British Columbia | Repressed trans-activator system for characterization of protein-protein interactions |
Non-Patent Citations (4)
Title |
---|
COLLER, JEFFERY M. ET AL: "mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation", GENES DEV. (1998), 12(20), 3226-3235, 15 October 1998 (1998-10-15), XP002122661 * |
MINVIELLE-SEBASTIA, LIONEL ET AL: "The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation", PROC. NATL. ACAD. SCI. U. S. A. (1997), 94(15), 7897-7902, 1997, XP002122760 * |
TARUN, SALVADOR, JR. ET AL: "Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G", EMBO J. (1996), 15(24), 7168-7177, 1996, XP002122662 * |
WELLS, SANDRA E. ET AL: "Circularization of mRNA by eukaryotic translation initiation factors", MOL. CELL (1998), 2(1), 135-140, 1998, XP002122664 * |
Also Published As
Publication number | Publication date |
---|---|
US6303311B1 (en) | 2001-10-16 |
AU3902099A (en) | 1999-12-06 |
WO1999060408A2 (en) | 1999-11-25 |
US5985575A (en) | 1999-11-16 |
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