WO1999060408A3 - Assay involving protein binding to 3' untranslated mrna sequences - Google Patents

Assay involving protein binding to 3' untranslated mrna sequences Download PDF

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Publication number
WO1999060408A3
WO1999060408A3 PCT/US1999/010531 US9910531W WO9960408A3 WO 1999060408 A3 WO1999060408 A3 WO 1999060408A3 US 9910531 W US9910531 W US 9910531W WO 9960408 A3 WO9960408 A3 WO 9960408A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein binding
mrna sequences
protein
assay involving
involving protein
Prior art date
Application number
PCT/US1999/010531
Other languages
French (fr)
Other versions
WO1999060408A2 (en
Inventor
Marvin P Wickens
Roy Parker
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Priority to AU39020/99A priority Critical patent/AU3902099A/en
Publication of WO1999060408A2 publication Critical patent/WO1999060408A2/en
Publication of WO1999060408A3 publication Critical patent/WO1999060408A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

A method for determining the function of a test protein is disclosed. In a preferred embodiment, this method comprises the steps of fusing a test protein to an RNA-binding protein and exposing the fusion protein to recombinant reporter mRNA molecule that comprises a binding site for the RNA-binding protein in the 3' untranslated region. One then observes the properties of reporter mRNA and correlates the reporter properties with test protein function.
PCT/US1999/010531 1998-05-20 1999-05-12 Assay involving protein binding to 3' untranslated mrna sequences WO1999060408A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU39020/99A AU3902099A (en) 1998-05-20 1999-05-12 Tethered function assay for protein function

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/081,731 1998-05-20
US09/081,731 US5985575A (en) 1998-05-20 1998-05-20 Tethered function assay for protein function

Publications (2)

Publication Number Publication Date
WO1999060408A2 WO1999060408A2 (en) 1999-11-25
WO1999060408A3 true WO1999060408A3 (en) 2000-01-20

Family

ID=22166026

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010531 WO1999060408A2 (en) 1998-05-20 1999-05-12 Assay involving protein binding to 3' untranslated mrna sequences

Country Status (3)

Country Link
US (2) US5985575A (en)
AU (1) AU3902099A (en)
WO (1) WO1999060408A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
JP3692542B2 (en) 1997-01-21 2005-09-07 ザ ジェネラル ホスピタル コーポレーション Protein selection using RNA-protein fusions
US8207093B2 (en) * 1997-01-21 2012-06-26 The General Hospital Corporation Selection of proteins using RNA-protein fusions
EP1068356B8 (en) * 1998-04-03 2007-01-03 Adnexus Therapeutics, Inc. Addressable protein arrays
EP1105360B1 (en) 1998-08-17 2009-07-29 Bristol-Myers Squibb Company Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules
WO2000009737A1 (en) * 1998-08-17 2000-02-24 Phylos, Inc. Methods for producing nucleic acids lacking 3'-untranslated regions and optimizing cellular rna-protein fusion formation
AU775997B2 (en) * 1998-12-02 2004-08-19 Bristol-Myers Squibb Company DNA-protein fusions and uses thereof
US6623926B1 (en) 1999-06-01 2003-09-23 Phylos, Inc. Methods for producing 5′-nucleic acid-protein conjugates
PT1870417E (en) * 1999-07-27 2012-06-01 Bristol Myers Squibb Co Peptide acceptor ligation methods
US7022479B2 (en) * 2000-01-24 2006-04-04 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
DE60118527D1 (en) 2000-01-24 2006-05-18 Compound Therapeutics Inc SENSITIVE AND MULTIPLEXED DIAGNOSTIC TESTS FOR PROTEIN ANALYSIS
IL134830A0 (en) * 2000-03-01 2001-05-20 Chay 13 Medical Res Group N V Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them
AU2001257428A1 (en) * 2000-04-28 2001-11-12 Message Pharmaceuticals, Inc. Methods for identifying novel nucleic acid regulatory elements and compounds that affect the regulation
US20060073481A1 (en) * 2002-04-23 2006-04-06 The Molecular Sciences Institute Formation and use of site specific nucleic acid coupled binding polypeptides
US20030198973A1 (en) * 2002-04-23 2003-10-23 The Molecular Sciences Institute, Inc. Chimeric fusion molecule for analyte detection and quantitation
US20040235001A1 (en) * 2002-09-18 2004-11-25 Liaoteng Wang Regulatory poly(A) polymerase and uses thereof
US7919583B2 (en) * 2005-08-08 2011-04-05 Discovery Genomics, Inc. Integration-site directed vector systems
JP2009008608A (en) * 2007-06-29 2009-01-15 Sysmex Corp Method and kit for determining amount of deoxyribonucleic acid (dna)-binding protein
CN108690139B (en) 2017-07-31 2019-03-15 康码（上海）生物科技有限公司 The preparation of new fusion protein and its application synthesized in raising protein
US11447783B2 (en) 2018-03-06 2022-09-20 Danisco Us Inc. Reduction in acetate production by yeast over-expressing PAB1

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037422A1 (en) * 1997-02-20 1998-08-27 Message Pharmaceuticals, Inc. Method for identifying binding sites for rna binding proteins
US5885779A (en) * 1997-09-09 1999-03-23 University Of British Columbia Repressed trans-activator system for characterization of protein-protein interactions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037422A1 (en) * 1997-02-20 1998-08-27 Message Pharmaceuticals, Inc. Method for identifying binding sites for rna binding proteins
US5885779A (en) * 1997-09-09 1999-03-23 University Of British Columbia Repressed trans-activator system for characterization of protein-protein interactions

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
COLLER, JEFFERY M. ET AL: "mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation", GENES DEV. (1998), 12(20), 3226-3235, 15 October 1998 (1998-10-15), XP002122661 *
MINVIELLE-SEBASTIA, LIONEL ET AL: "The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation", PROC. NATL. ACAD. SCI. U. S. A. (1997), 94(15), 7897-7902, 1997, XP002122760 *
TARUN, SALVADOR, JR. ET AL: "Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G", EMBO J. (1996), 15(24), 7168-7177, 1996, XP002122662 *
WELLS, SANDRA E. ET AL: "Circularization of mRNA by eukaryotic translation initiation factors", MOL. CELL (1998), 2(1), 135-140, 1998, XP002122664 *

Also Published As

Publication number Publication date
US5985575A (en) 1999-11-16
US6303311B1 (en) 2001-10-16
AU3902099A (en) 1999-12-06
WO1999060408A2 (en) 1999-11-25

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