WO1999058663A9 - METHODS AND COMPOSITIONS FOR SCREENING FOR MODULATORS OF IgE SYNTHESIS, SECRETION AND SWITCH REARRANGEMENT - Google Patents
METHODS AND COMPOSITIONS FOR SCREENING FOR MODULATORS OF IgE SYNTHESIS, SECRETION AND SWITCH REARRANGEMENTInfo
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- WO1999058663A9 WO1999058663A9 PCT/US1999/010497 US9910497W WO9958663A9 WO 1999058663 A9 WO1999058663 A9 WO 1999058663A9 US 9910497 W US9910497 W US 9910497W WO 9958663 A9 WO9958663 A9 WO 9958663A9
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
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- C12N2510/00—Genetically modified cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Definitions
- the invention relates to methods and compositions useful in screening for modulators of IgE synthesis, secretion and switch rearrangement
- Immunoglobulins must bind to a vast array of foreign molecules and thus exist in many forms
- the sequence of the variable (V) region of immunoglobulin molecules varies tremendously, conferring virtually unlimited capacity to bind antigens
- the constant (C) region comes in five different varieties ⁇ , ⁇ , e, Y and ⁇ , providing five different isotypes IgA, IgD, IgE, IgG and IgM, each of which performs a different set of functions
- B cells initially produce only IgM and IgD, and must be activated or induced to produce the other isoforms such as IgE
- B cells Upon activation with an antigen, B cells follow one of two differentiation pathways they may differentiate directly into plasma cells, which are basically antibody-secreting factories, or they may give rise to germinal centers, specialized structures within lymphoid organs In the latter, successive rounds of mutation of the V region genes is followed by expression of the gene products on the cell surface, with selection of the cells on the basis of the affinity of the mutated immunoglobulins against the antigen
- isotype switching occurs in which the C region of the immunoglobulin heavy chain changes from the joint expression of IgM and IgD on naive B cells to expression of one of the downstream isotypes such as IgE
- This switching involves the replacement of upstream C regions with a downstream C region that has biologically distinct effector functions without changing the structure of the variable portion and, hence, its specificity
- a deletional rearrangement of the Ig heavy chain gene locus occurs, a rearrangement that joins the switch region of the ⁇ gene, S ⁇ , with the corresponding region of the e gene, Se
- This switching is minimally induced by IL-4 or IL-13, which initates transcription through the Se region, resulting in the synthesis of germ-line (or "sterile") e transcripts, that is, transcripts of the unrearranged C e heavy genes
- This IL-4 induced transcription is inhibited by IFN- ⁇ , IFN- ⁇ , and TGF- ⁇
- a second signal normally
- IgE antibodies are crucial immune mediators of allergic reactions, and have been shown to be responsible for the induction and maintenance of allergic symptoms For example, the introduction of anti-lgE antibodies has been shown to interfere with IgE function, thus working to alleviate allergic symptoms See Jardieu, Current Op Immunol 7 779-782 (1995), Shields et al , Int Arch Allergy Immunol 107 308-312 (1995)
- the present invention provides methods of screening for bioactive agents capable of inhibiting an IL-4 inducible e promoter
- the method comprises combining a candidate bioactive agent and a cell comprising a fusion nucleic acid
- the fusion nucleic acid comprises an IL-4 inducible e promoter, and a reporter gene
- the promoter is then induced with IL-4 (or IL-13), and the presence or absence of the reporter protein is detected Generally, the absence of the reporter protein indicates that the agent inhibits the IL-4 inducible e promoter
- the fusion nucleic acid may comprise an exogeneous IL-4 inducible e promoter, or an endogeneous IL-4 inducible e promoter
- Preferred embodiments utilize the use of retroviral vectors to introduce the candidate bioactive agents
- the present invention provides cell lines for screening Either CA-46 and MC- 116 cell lines are included, and further comprise fusion nucleic acids comprising an IL-4 inducible e promoter, and
- the present invention provides methods of screening for bioactive agents capable of modulating IgE production
- the method comprises combining a candidate bioactive agent and a cell capable of expressing IgE and determining the amount of IgE produced in the cell Generally, a change in the amount of IgE as compared to the amount produced in the absence of the candidate agent indicates that the agent modulates IgE production
- the cell can further comprise a IgE fusion protein comprises the e heavy chain, and a fluorescent protein
- the invention provides methods of screening for bioactive agents capable of inhibiting a promoter of interest
- the method comprises combining a candidate bioactive agent and a cell comprising a fusion nucleic acid
- the fusion nucleic acid comprises a promoter of interest and a reporter gene comprising a death gene that is activated by the introduction of a ligand
- the promoter is optionally induced, and the ligand is introduced to the cell
- the presence of the cell is then detected, wherein the presence of the cell indicates that the agent inhibits the promoter
- the invention provides compositions comprising a test vector and a reporter vector
- the test vector comprises a first selection gene, and a fusion gene comprising a first sequence encoding a transcriptional activation domain, and a second sequence encoding a test protein
- the reporter vector comprises a first detectable gene, and all or part of the switch e sequence, which upon binding of the transcriptional activation domain due to a protein-nucleic acid interaction between the test protein and the switch e sequence, will activate transcription of the first detectable gene
- Methods utilizing these compositions are also provided, the methods comprise providing a host cell comprising the composition, and subjecting the host cell to conditions under which the fusion gene is expressed to produce a fusion protein A protein-nucleic acid interaction between the fusion protein and the switch e sequence is then detected
- Figures 1 A and 1 B depict the germ ne e locus and sequence Fig 1A depicts the sequence of the human IL-4 inducible e promoter Fig 1 B depicts the organization of the germhne e locus
- FIGS 2A and 2B depict the regions (2A) and sequences (2B and 2C) of the switch e (Se) region that are used in methods of screening for proteins that interact with the Se region, as described below
- Figure 3 shows a schematic of the yeast one-hybrid system used to identify proteins that bind to the Se region
- Figure 4 depicts the IL-4 induction of germhne e mRNA in three lgM + B cell lines, CA-46, MC-116 and DND39 The cells were incubated for 48 hours in 300 U/ml of hlL-4 RT-PCR ws performed using p ⁇ miers specific for the germhne e exon and the 5'-end of the e CH1 exon (predicted size is -200 bp)
- Figures 5A, 5B, 5C and 5D depict two general approaches to generate germhne e promoter knock-in reporter cell lines
- Figure 5A shows the organization of this region in vivo
- Figures 5B and 5C depict two possible knock in constructs
- the IL-4 inducible lgM+ B cell lines are transfected with one or both of these constructs Under the influence of IL-4, GFP and/or BFP positive clones are isolated by FACS Homologous recombination can be confirmed by PCR and/or Southern blot hybridization
- Figure 5D depicts an alternate construct
- the IL-4 inducible lgM+ B cell lines are transfected with the 5D construct and selected with G418 Survivors are sorted for the lack of the 3' BFP expression (deleted during homologous recombination) RT-PCR is performed to confirm homologous recombination Those clones are transfected with ere to remove the neomycin resistance gene
- Figure 7 depicts a general schematic of the generation of the primary peptide libraries in retroviruses
- Figures 8A and 8B depict constructs useful in generating e heavy chain knock-in cell lines
- Figure 8A depicts the wild-type organization
- GFP green fluorescent protein
- BFP blue fluorescent protein
- Neo r neomycin resistance gene
- VDJ V region exon
- CH1 , 2, 3, 4 constant region domain exons
- M1 , M2 membrane exons
- HSV-TK Herpes Simplex Virus - thymidine kinase
- Figures 9A and 9B depict constructs useful in the invention
- Figure 9A shows a reporter construct useful to create an IL-4 inducible e promoter reporter cell line
- CRU5 hCMV pormoter plus R and U5 regions of LTR
- BGH poly A bovine growth hormone poly-adenylation signal
- SIN self-inactivating LTR
- Figure 9B shows a library construct
- Figures 10A and 10B depict a schematic of the screen for candidate agents of the germhne e promoter Figure 10A the experimental schematic Figure 10B depicts the survival construct useful in the screen Position 1 can be a number of different genes, including a FAS chimeric receptor outlined herein (including extracellular mouse Fas receptor or mouse CD8 receptor coupled with the human transmembrane and cytoplasmic Fas receptor), HSV-TK, p450 2B1 and p21 peptide
- FIGS 11 A 11 B and 11C depict preferred vectors and their sequences
- Figures 12A 12B and 12C depict a construct useful in the present invention, comprising the a Fas survival construct (i e the use of a death gene)
- the sequence is of the inducible e promoter-chimeric
- FIGS 13A 13B and 13C depict a construct useful in the present invention, comprising the a Fas survival construct (i e the use of a death gene)
- the sequence is of the inducible e promoter-chimeric Fas (either CD8 or mLyt2)-IRES-hygromyc ⁇ n-bov ⁇ ne growth hormone poly A tail that is put into the
- the present invention provides compositions and methods useful in screening for modulators, particularly inhibitors, of the production of IgE antibodies
- assay methodologies are provided that are amenable to high-throughput screening strategies, such that large numbers of potential drugs may be screened rapidly and efficiently
- traditional treatments for IgE suppression are based on regulation of the system after IgE has been made, for example using anti- IgE antibodies or anti-histamines, to modulate the IgE-mediated response resulting in mast cell degranulation
- drugs are known that generally downregulate IgE production or that inhibit switching but not induction of germhne transcripts (see for example Loh et al , J Allerg Chn
- the present invention provides several related techniques that may be used to screen for upstream modulators of IgE production, to prevent the production of IgE and thus reduce or eliminate the allergic response
- an early step in the Ig switch is the production of sterile e transcripts in response to IL-4
- blockage of the production of membrane bound IgE may induce programmed cell death (PCD)
- PCD programmed cell death
- these techniques allow individual cell assessment and thus are useful for high-throughput screening strategies, for example those that utilize fluorescence activated cell sorting (FACS) techniques, and thus allow screening of large numbers of compounds for their effects on IgE production
- the invention relates to methods that rely on reporter genes fused to IgE promoters, such as the IL-4 inducible e promoter that starts a cascade that ultimately results in IgE production Using novel reporter constructs, screening for modulators of this promoter system may be done
- novel reporter constructs screening for modulators of this promoter system may be done
- the invention provides a number of different constructs that allow for screening for antagonists and agonists of these promoters
- the invention provides methods of screening for bioactive agents capable of modulating, particularly inhibiting, an IL-4 inducible e promoter
- an IL-4 inducible promoter herein is meant a nucleic acid promoter that is induced by IL-4, putatively by binding an unknown IL-4 induced DNA binding protein that results in induction of the promoter, that is, the introduction of IL-4 causes the pronounced activation of a particular DNA binding protein that then binds to the IL-4 inducible promoter segment and induces transcription
- the sequence of the human IL-4 inducible promoter is shown in Figure 1, and as will be appreciated by those in the art, derivatives or mutant promoters are included within this definition Particularly included within the definition of an IL-4 inducible promoter are fragments or deletions of the sequence shown in Figure 1
- the IL-4 inducible promoter is also inducible by IL-13
- modulating an IL-4 inducible promoter herein is meant either an increase or a
- the methods comprise combining a candidate bioactive agent and a cell or a population of cells comprising a fusion nucleic acid
- the cell or cells comprise a fusion nucleic acid
- the fusion nucleic acid comprises an IL-4 inducible e promoter and at least a first reporter gene
- the IL-4 inducible e promoter is as described herein, for example SEQ ID NO 1 , or derivatives thereof, and may be either an endogeneous or exogeneous IL-4 inducible e promoter, as is more fully described below
- reporter gene or “selection gene” herein is meant a gene that by its presence in a cell (i e upon expression) can allow the cell to be distinguished from a cell that does not contain the reporter gene Reporter genes can be classified into several different types, including detection genes, survival genes, death genes and cell cycle genes It may be the nucleic acid or the protein expression product that causes the effect As is more fully outlined below, additional components, such as substrates, hgands, etc , may be additionally added to allow selection or sorting on the basis of the reporter gene
- the reporter gene encodes a protein that can be used as a direct label, i e a detection gene, for sorting the cells, i e for cell enrichment by FACS
- the protein product of the reporter gene itself can serve to distinguish cells that are expressing the reporter gene
- suitable reporter genes include those encoding green fluorescent protein (GFP, Chalfie, et al , "Green Fluorescent Protein as a Marker for Gene Expression," Science 263(5148) 802-805 (Feb 11 , 1994), and EGFP, Clontech - Genbank Accession Number U55762 ), blue fluorescent protein (BFP, 1 Quantum Biotechnologies, Inc 1801 de Maisonneuve Blvd West, 8th
- the reporter gene encodes a protein that will bind a label that can be used as the basis of the cell enrichment (sorting), i e the reporter gene serves as an indirect label or detection gene
- the reporter gene should encode a cell-surface protein
- the reporter gene may be any cell-surface protein not normally expressed on the surface of the cell, such that secondary binding agents could serve to distinguish cells that contain the reporter gene from those that do not Alternatively, albeit non-preferably, reporters comprising normally expressed cell-surface proteins could be used, and differences between cells containing the reporter construct and those without could be determined
- secondary binding agents bind to the reporter protein
- These secondary binding agents are preferably labelled, for example with fluors, and can be antibodies, haptens, etc
- fluorescently labeled antibodies to the reporter gene can be used as the label
- membrane-tethered streptavidin could serve as a reporter gene
- fluorescently- labeled biotin could be used as the label, i e the secondary binding agent
- the secondary binding agents are preferably labelled,
- the reporter gene is a cell cycle gene, that is, a gene that causes alterations in the cell cycle
- p21 protein its ligand a collection of three proteins, see Harper, et ai , "The p21 Cdk-lnteractmg Protein C ⁇ p1 Is a Potent Inhibitor of G1 Cychn-Dependent Kinases," Cell 75 805-816 (November 19, 1993)
- p21 protein its ligand a collection of three proteins, see Harper, et ai , "The p21 Cdk-lnteractmg Protein C ⁇ p1 Is a Potent Inhibitor of G1 Cychn-Dependent Kinases," Cell 75 805-816 (November 19, 1993)
- cell-cycle arrest such that cells containing inhibited IL-4 inducible promoters grow out much more quickly, allowing detection on this basis
- the reporter gene is a death gene that provides a nucleic acid that encodes a protein that causes the cells to die
- Death genes fall into two basic categories death genes that encode death proteins that require a death ligand to kill the cells, and death genes that encode death proteins that kill cells as a result of high expression within the cell, and do not require the addition of any death ligand
- cell death requires a two-step process the expression of the death gene and induction of the death phenotype with a signal or ligand, such that the cells may be grown up expressing the death gene, and then induced to die
- a number of death genes/hgand pairs are known including, but not limited to, the Fas receptor and Fas ligand (Bodmer, et al , "Characterization of Fas " J Biol Chem 272(30) 18827-18833 (Jul 25, 1997), muFAS, Gonzalez- Cuadrado, et al , "Agonistic anti-Fas Antibodies In
- P450/Cyclophospham ⁇ de-Based Cancer Gene Therapy By Coexpression of the P450 Reductase Gene," Cancer Res 57(21 ) 4830-4837 (Nov 1 1997)), thymidine kinase and gangcylovir (Stone, R , "Molecular 'Surgery' For Brain Tumors," 256(5063) 1513 (June 12, 1992)), tumor necrosis factor (TNF) receptor and TNF
- the death gene need not require a ligand, and death results from high expression of the gene, for example, the overexpression of a number of programmed cell death (PCD) proteins are known to cause cell death, including, but not limited to, caspases, bax, TRADD, FADD, SCK, MEK, etc.
- PCD programmed cell death
- the use of the death genes in the manner described herein, particularly in two-step applications, allows general and high-throughput screening for inhibitors of other promoters, in addition to the IL-4 inducible e promoters described herein
- the present invention provides fusion nucleic acids comprising a promoter of interest operably linked to a death gene for use in screening methods
- the promoter of interest can be either a constitutive promoter or an inducible promoter, such as the IL-4 inducible e promoter
- any number of possible promoters could be used Suitable promoters of interest include, but are not limited to, inducible promoters such as IL-4 e promoter, promoters that are induced by cytokines or growth factors such as the interferon responsive factors 1 to 4, NFkB (Fie ⁇ ng, et al , "Single Cell Assay of a Transcription Factor Reveals a Threshold in Transcription
- the promoter of interest is a constitutive promoter, and it is hooked to a death gene that requires the presence of a ligand, such as Fas or TNF
- a ligand such as Fas or TNF
- the cells can be grown up and the presence of the death gene verified due to the constitutive promoter
- a detection gene such as GFP or BFP, etc
- the presence of the detection gene means the death gene is also present Verification of the presence of the death gene is preferred to keep the levels of false positives low, that is, cells that survive the screen should be due to the presence of an inhibitor of the promoter rather than a lack of the death gene
- the candidate agents can be added (and their presence verified as well), followed by induction in the presence of IL-4, and finally by addition of the death ligand
- the cell population is enriched for those cells that have an agent that inhibits the promoter and thus does not produce the death protein, i e those that survive
- a preferred embodiment utilizes fusion nucleic acids comprising promoters of interest that are inducible (such as the IL-4 e promoter), and hooked to a death gene that requires a death ligand The presence of the death gene is verified by inducing the promoter, causing the death gene (and preferably a detection gene) to be made The candidate agents and death gands are then introduced in the presence of their appropriate inducer, and the population is enriched for those cells that survive, i e contain an agent that inhibits the promoter and thus does not produce the death protein
- chimeric death genes When death genes that require hgands are used, i e for "two step" processes, preferred embodiments utilize chimeric death genes, i e chimeric death receptor genes These chimeric death receptors comprise the extracellular domain of a hgand-activated multime ⁇ zing receptor and the endogeneous cytoso c domain of a death receptor gene, such as Fas or TNF This is done to avoid endogeneous activation of the death gene
- the mechanism of Fas-induced cell death involves the introduction of the Fas ligand, which can bind two monomeric Fas receptors, causing the multime ⁇ zation of the receptor, which activates the receptor and leads to secondary signalling resulting in caspase activation and PCD
- Fas ligand which can bind two monomeric Fas receptors, causing the multime ⁇ zation of the receptor, which activates the receptor and leads to secondary signalling resulting in caspase activation and PCD
- chimeric Fas receptor genes are made The exact combination will depend on the cell type used and the receptors normally produced by these cells For example, when using human cells or cell lines, a non-human extracellular domain and a human cytosohc domain are preferred, to prevent endogeneous induction of the death gene
- a preferred embodiment utilizes human cells, a murine extracellular Fas receptor domain and a human cytosohc domain, such that the endogeneous human Fas ligand will not activate the murine domain
- human extracellular domains may be used when the cells used do not endogeneously produce the ligand, for example, the human EPO extracellular domain may be used when the cells do not endogeneously produce EPO (Kawaguchi, et al , Cancer Lett , 116(1) 53 (1997), Takebayashi, et al , Cancer Res , 56(18) 4164 (1996), Rudert, et ai , Biochem Biophys Res Commun ,
- transmembrane domain from any of the receptors can be used, although in general, it is preferred to use the transmembrane domain associated with the chosen cytosohc domain, to preserve the interaction of the transmembrane domain with other endogeneous signalling proteins
- preferred embodiments provide fusion nucleic acids that utilize the IL-4 inducible e promoter linked to a death gene, particularly a chimeric death receptor gene, that requires a death ligand for cell killing
- inducible promoters can be linked to "one step" death genes, i e death genes that upon a certain threshold expression, will kill a cell without requiring a ligand or secondary signal
- the inducible promoter is preferably "leaky", such that some small amount of death gene and a required secondary reporter gene such as a survival gene or a detection gene can be expressed
- the cells that contain the death gene can then be selected on this basis, to avoid false positives
- candidate agents are added (and their presence preferably verified using a detection or selection gene as well), and the promoter is induced
- the population is then enriched for those cells that contain agents that inhibit the promoter, i e that will survive
- additional reporter genes are used, particularly when inducible death genes are used
- the additional reporter gene is a selection gene The cells containing the death gene and the drug selectable gene are grown, if the appropriate drug is added to the culture, only those cells containing the resistance gene (and hence the death gene) survive This ensures that the cells are expressing the death gene to decrease "false positives", i e cells that do not die because they do not contain the death gene
- the additional reporter gene is a labeling gene such as GFP
- a detection gene allows cells to be sorted to give a population enriched for those containing the construct
- a preferred embodiment uses "leaky” inducible promoters, that is, the cells are selected such that the IL-4 inducible promoter, even in the absence of IL-4 or IL-13, produces some GFP and death gene (for example, the Fas receptor constructs)
- suitably "leaky” promoters are chosen such that some GFP is expressed (preferably enough to select the cells expressing the construct from those that are not), but not enough death gene is produced to cause death
- preferred embodiments utilize death genes requiring the addition of a death ligand, it is well known that high levels of some death genes, even in the absence of death ligand, can cause death Thus, for example, high levels of Fas receptor expression can cause multime ⁇ zation, and thus activation, even in the absence of the Fas ligand
- Encephalomycarditis Virus Molecular and Cellular Biology 12(8) 3636-3643 (Aug 1992) and McBratney, et al , "The Sequence Context of the Initiation Codon in the Encephalomycarditis Virus Leader Modulates Efficiency of Internal Translation Initiation," Current Opinion in Cell Biology 5 961- 965 (1993)), or a protease cleavage site (which cleaves a protein translation product into two proteins)
- Preferred protease cleavage sites include, but are not limited to, the 2a site (Ryan et al , J
- the fusion nucleic acids may comprise additional components, including, but not limited to, other reporter genes, protein cleavage sites internal ribosome entry (IRES) sites, AP-1 sites, and other components as will be appreciated by those in the art
- foreign constructs comprising the IL-4 inducible e promoter and the reporter gene are made
- foreign herein is meant that the fusion nucleic acids originates outside of the cells That is, a recombinant nucleic acid is made that contains an exogeneous IL-4 inducible e promoter and a reporter gene Thus, in some circumstances, the cells will contain both exogeneous and endogeneous IL-4 inducible e promoters
- recombinant nucleic acid herein is meant nucleic acid, originally formed in vitro in general, by the manipulation of nucleic acid by endonucleases, in a form not normally found in nature
- an isolated nucleic acid, in a linear form, a nucleic acid containing components not normally joined, such as an IL-4 inducible promoter and a reporter gene, or an expression vector formed in vitro by hgating DNA molecules that are not normally joined are all considered recombinant for the purposes of this invention It is
- This recombinant nucleic acid may introduced to a cell in a variety of ways, as will be appreciated by those in the art, including, but not limited to, CaP0 4 precipitation, hposome fusion, hpofectin®, electroporation, viral infection etc
- the constructs may preferably stably integrate into the genome of the host cell (for example, with retroviral introduction, outlined below), or may exist either transiently or stably in the cytoplasm (i e through the use of traditional plasmids, utilizing standard regulatory sequences, selection markers etc )
- exogeneous constructs which may be in the form of an expression vector, are added as retroviral constructs, using techniques generally described in PCT US97/01019 and PCT US97/01048, both of which are expressly incorporated by reference, and the examples
- the fusion construct comprises an endogeneous IL-4 inducible e promoter and an exogeneous reporter gene
- endogeneous in this context means originating within the cell That is, gene “knock-in” constructions are made, whereby an exogeneous reporter gene as outlined herein is added, via homologous recombination, to the genome, such that the reporter gene is under the control of the endogeneous IL-4 inducible e promoter
- This may be desirable to allow for the exploration and modulation of the full range of endogeneous regulation, i e regulatory elements (particularly those flanking the promoter) other than just the IL-4 inducible e promoter fragment
- Exemplary constructs are shown in Figures 5B and 5C, with GFP and BFP, although other reporter genes outlined herein may be used
- Homologous recombination may proceed in several ways In one embodiment, traditional homologous recombination is done, with molecular biological techniques such as PCR being done to find the correct insertions
- gene "knock-ins” may be done as is known in the art, for example see Westphal et al , Current Biology 7 R530-R533 (1997), and references cited therein, all of which are expressly incorporated by reference
- the use of recA mediated systems may also be done, see PCT US93/03868, hereby expressly incorporated by reference
- a first homologous recombination event is done to put a first reporter gene, such as GFP, into at least one allele of the cell genome
- a first reporter gene such as GFP
- this is a cell type that exhibits IL-4 inducible production of at least germhne ⁇ transcripts, so that the cells may be tested by IL-4 production for reporter gene expression
- Suitable cells include, but are not limited to, human cells and cell lines that show IL-4/13 inducible production of germhne e transcripts, including, but not limited to, DND39 (see Watanabe, supra), MC-116, (Kumar, et al ,
- a second homologous recombination event may be done, preferably using a second reporter gene different from the first, such as BFP, to target the other allele of the cell genome, and tested as above
- IL-4 induction of the reporter genes will indicate the correct placement of the genes, which can be confirmed via sequencing such as PCR sequencing or Southern blot hybridization
- preferred embodiments utilize prescreening steps to remove "leaky” cells, i e those showing constitutive expression of the reporter gene
- the invention provides cell lines that contain fusion nucleic acids comprising IL-4 inducible e promoter operably connected to at least one reporter gene
- the cell lines comprising these reporter constructs are used to screen candidate bioactive agents for the ability to modulate the production of IgE, as is outlined below
- candidate bioactive agent or “exogeneous compound” as used herein describes any molecule, e g , protein, oligopeptide, small organic molecule, polysaccha ⁇ de, polynudeotide Generally a plurality of assay mixtures are run in parallel with different agent concentrations to obtain a differential response to the various concentrations Typically, one of these concentrations serves as a negative control, / e , at zero concentration or below the level of detection
- Candidate agents encompass numerous chemical classes, though typically they are organic molecules, preferably small organic compounds having a molecular weight of more than 100 and less than about 2,500 daltons
- Candidate agents comprise functional groups necessary for structural interaction with proteins, particularly hydrogen bonding, and typically include at least an amine, carbonyl, hydroxyl or carboxyl group, preferably at least two of the functional chemical groups
- the candidate agents often comprise cyclical carbon or heterocychc structures and/or aromatic or polyaromatic structures substituted with one or more of the above functional groups
- Candidate agents are also found among biomolecules including peptides, sacchandes, fatty acids, steroids, pu ⁇ nes, py ⁇ midines, derivatives, structural analogs or combinations thereof Particularly preferred are peptides
- Candidate agents are obtained from a wide variety of sources including libraries of synthetic or natural compounds For example, numerous means are available for random and directed synthesis of a wide variety of organic compounds and biomolecules, including expression of randomized oligonucleotides Alternatively, libraries of natural compounds in the form of bacterial, fungal, plant and animal extracts are available or readily produced Additionally, natural or synthetically produced libraries and compounds are readily modified through conventional chemical, physical and biochemical means Known pharmacological agents may be subjected to directed or random chemical modifications, such as acylation, alkylation, este ⁇ fication, amidification to produce structural analogs
- the candidate bioactive agents are proteins
- protein herein is meant at least two covalently attached ammo acids, which includes proteins, polypeptides, ohgopeptides and peptides
- the protein may be made up of naturally occurring ammo acids and peptide bonds, or synthetic peptidomimetic structures
- “ammo acid”, or “peptide residue”, as used herein means both naturally occurring and synthetic ammo acids
- homo-phenylalanine, citrulhne and noreleucme are considered ammo acids for the purposes of the invention
- Amo acid also includes imino acid residues such as prohne and hydroxyprohne
- the side chains may be in either the (R) or the (S) configuration
- the am o acids are in the (S) or L-configuration If non-naturally occurring side chains are used, non-ammo acid substituents may be used, for example to prevent or retard in vivo degradations
- the candidate bioactive agents are naturally occu ⁇ ng proteins or fragments of naturally occunng proteins
- cellular extracts containing proteins, or random or directed digests of protemaceous cellular extracts may be used
- libraries of procaryotic and eucaryotic proteins may be made for screening in the systems described herein
- Particularly preferred in this embodiment are libraries of bacterial, fungal, viral, and mammalian proteins, with the latter being preferred, and human proteins being especially preferred
- the candidate bioactive agents are peptides of from about 5 to about 30 ammo acids, with from about 5 to about 20 ammo acids being preferred, and from about 7 to about 15 being particularly preferred
- the peptides may be digests of naturally occunng proteins as is outlined above, random peptides, or "biased” random peptides
- randomized or grammatical equivalents herein is meant that each nucleic acid and peptide consists of essentially random nudeotides and ammo acids, respectively Since generally these random peptides (or nucleic acids, discussed below) are chemically synthesized they may incorporate any nucleotide or ammo acid at any position
- the synthetic process can be designed to generate randomized proteins or nucleic acids, to allow the formation of all or most of the possible combinations over the length of the sequence, thus forming a library of randomized candidate bioactive protemaceous agents
- the library is fully randomized, with no sequence preferences or constants at any position
- the library is biased That is, some positions within the sequence are either held constant, or are selected from a limited number of possibilities
- the nudeotides or ammo acid residues are randomized within a defined class, for example, of hydrophobic ammo acids, hydrophilic residues, ste ⁇ cally biased (either small or large) residues, towards the creation of cystemes, for cross-linking, pro nes for SH-3 domains, sennes, threon es, tyrosmes or histidines for phosphorylation sites, etc , or to pu ⁇ nes, etc
- the candidate bioactive agents are nucleic acids
- nucleic acid or “oligonucleotide” or grammatical equivalents herein means at least two nudeotides covalently linked together
- a nucleic acid of the present invention will generally contain phosphodiester bonds, although in some cases, as outlined below, nucleic acid analogs are included that may have alternate backbones, comprising, for example, phosphoramide (Beaucage, et al , Tetrahedron, 49(10) 1925
- nucleic acids include those with positive backbones (Denpcy, ef al , Proc Natl Acad Sci USA. 92 6097 (1995)), non-ionic backbones (U S Patent Nos 5,386,023, 5,637,684, 5,602,240, 5,216,141 , and 4 469,863, Kiedrowshi, ef al , An ⁇ ew Chem Intl Ed English. 30 423 (1991 ), Letsinger, ef al . J Am Chem Soc , 110 4470 (1988), Letsinger, ef al , Nucleoside &
- nucleic acids containing one or more carbocydic sugars are also included within the definition of nucleic acids (see Jenkins, ef al , Chem Soc Rev , (1995) pp 169-176)
- nucleic acid analogs are described in Rawls, C & E News, June 2, 1997, page 35 All of these references are hereby expressly incorporated by reference
- These modifications of the ⁇ bose-phosphate backbone may be done to facilitate the addition of additional moieties such as labels, or to increase the stability and half-life of such molecules in physiological environments
- mixtures of naturally occurring nucleic acids and analogs can be made Alternatively mixtures of different nucleic acid analogs, and mixtures of naturally occunng nucleic acids and analogs may be made
- the nucleic acids may be single stranded or double stranded, as specified, or contain portions of both double stranded or single stranded sequence
- the nucleic acid may be DNA
- nucleic acid candidate bioactive agents may be naturally occunng nucleic acids, random nucleic acids, or "biased" random nucleic acids
- digests of procaryotic or eucaryotic genomes may be used as is outlined above for proteins
- the candidate bioactive agents are organic chemical moieties, a wide variety of which are available in the literature
- a library of different candidate bioactive agents are used
- the library should provide a sufficiently structurally diverse population of randomized agents to effect a probabilistically sufficient range of diversity to allow binding to a particular target
- an interaction library should be large enough so that at least one of its members will have a structure that gives it affinity for the target
- it is difficult to gauge the required absolute size of an interaction library nature provides a hint with the immune response a diversity of 10 7 -10 8 different antibodies provides at least one combination with sufficient affinity to interact with most potential antigens faced by an organism
- Published in vitro selection techniques have also shown that a library size of 10 7 to 10 8 is sufficient to find structures with affinity for the target
- a library of all combinations of a peptide 7 to 20 am o acids in length, such as generally proposed herein, has the potential to code for
- the candidate bioactive agents are combined or added to a cell or population of cells Suitable cell types for different embodiments are outlined above
- population of cells herein is meant at least two cells with at least about 10 5 being preferred, at least about 10 6 being particularly preferred, and at least about 10 7 , 10 8 and 10 9 being especially preferred
- the candidate bioactive agent and the cells are combined as will be appreciated by those in the art this may accomplished in any number of ways, including adding the candidate agents to the surface of the cells, to the media containing the cells, or to a surface on which the cells are growing or in contact with, adding the agents into the cells, for example by using vectors that will introduce the agents into the cells (i e when the agents are nucleic acids or proteins)
- a library of retroviral vectors is made using retroviral packaging cell lines that are helper-defective and are capable of producing all the necessary trans proteins, including gag, pol and env, and RNA molecules that have in cis the ⁇ packaging signal
- the library is generated in a retrovirus DNA construct backbone, standard oligonucleotide synthesis is done to generate either the candidate agent or nucleic acid encoding a protein, for example a random peptide, using techniques well known in the art
- the library is cloned into a first primer
- the first primer serves as a "cassette", which is inserted into the retroviral construct
- the first primer generally contains a number of elements, including for example
- a second primer is then added, which generally consists of some or all of the complementarity region to prime the first primer and optional necessary sequences for a second unique restriction site for subcloning DNA polymerase is added to make double-stranded oligonucleotides
- the double- stranded oligonucleotides are cleaved with the appropriate subcloning restriction endonucleases and subcloned into the target retroviral vectors, described below
- retroviral vectors may include selectable marker genes under the control of internal ribosome entry sites (IRES) that greatly facilitates the selection of cells expressing peptides at uniformly high levels, and promoters driving expression of a second gene, placed in sense or anti-sense relative to the 5' LTR
- IRS internal ribosome entry sites
- Suitable selection genes include, but are not limited to, neomycin, blastocidm, bleomycin, puromycin, and hygromycin resistance genes, as well as self-fluorescent markers such as green fluoroscent protein, enzymatic markers such as lacZ, and surface proteins such as CD8, etc
- Preferred vectors include a vector based on the murine stem cell virus (MSCV) (see Hawley et al , Gene Therapy 1 136 (1994)) and a modified MFG virus (Rivere et al , Genetics 92 6733 (1995)), and pBABE, outlined in the examples
- the retroviruses may include inducible and constitutive promoters for the expression of the candidate agent (to be distinguished from the IL-4 inducible e promoter)
- inducible and constitutive promoters for the expression of the candidate agent (to be distinguished from the IL-4 inducible e promoter)
- the candidate bioactive agents are linked to a fusion partner
- fusion partner or “functional group” herein is meant a sequence that is associated with the candidate bioactive agent, that confers upon all members of the library in that class a common function or ability Fusion partners can be heterologous (i e not native to the host cell), or synthetic (not native to any cell)
- Suitable fusion partners include, but are not limited to a) presentation structures, as defined below, which provide the candidate bioactive agents in a conformationally restricted or stable form, b) targeting sequences, defined below, which allow the localization of the candidate bioactive agent into a subcellular or extracellular compartment, particularly a nuclear localization sequence (NLS), c) rescue sequences as defined below, which allow the purification or isolation of either the candidate bioactive agents or the nucleic acids encoding them, d) stability sequences, which confer stability or protection from degradation to the candidate bioactive agent or the nucleic acid encoding it, for example resistance to proteolytic degradation, e) dime ⁇ z
- the fusion partner is a presentation structure
- presentation structure or grammatical equivalents herein is meant a sequence, which, when fused to candidate bioactive agents, causes the candidate agents to assume a conformationally restricted form Proteins interact with each other largely through conformationally constrained domains
- small peptides with freely rotating ammo and carboxyl termini can have potent functions as is known in the art, the conversion of such peptide structures into pharmacologic agents is difficult due to the inability to predict side-chain positions for peptidomimetic synthesis Therefore the presentation of peptides in conformationally constrained structures will benefit both the later generation of pharmaceuticals and will also likely lead to higher affinity interactions of the peptide with the target protein This fact has been recognized in the combinatorial library generation systems using biologically generated short peptides in bacterial phage systems A number of workers have constructed small domain molecules in which one might present randomized peptide structures
- presentation structures are preferably used with peptide candidate agents
- synthetic presentation structures i e artificial polypeptides
- presentation structures comprise a first portion joined to the N-termmal end of the randomized peptide, and a second portion joined to the C-termmal end of the peptide, that is, the peptide is inserted into the presentation structure, although variations may be made, as outlined below
- the presentation structures are selected or designed to have minimal biologically activity when expressed in the target cell
- suitable presentation structures maximize accessibility to the peptide by presenting it on an exterior loop
- suitable presentation structures include, but are not limited to, mmibody structures, loops on beta-sheet turns and coiled-coil stem structures in which residues not critical to structure are randomized, zinc-finger domains, cysteine-lmked (disulfide) structures, transglutaminase linked structures, cyclic peptides, B-loop structures, helical barrels or bundles, leucine zipper motifs, etc
- the presentation structure is a coiled-coil structure, allowing the presentation of the randomized peptide on an exterior loop
- coiled-coil structures allow for between 6 to 20 randomized positions
- a preferred coiled-coil presentation structure is as follows
- the underlined regions represent a coiled-coil leucine zipper region defined previously (see Martin et al , EMBO J 13(22) 5303-5309 (1994), incorporated by reference)
- the bolded GRGDMP region represents the loop structure and when appropriately replaced with randomized peptides (i e candidate bioactive agents, generally depicted herein as (X) n , where X is an ammo acid residue and n is an integer of at least 5 or 6) can be of variable length
- the replacement of the bolded region is facilitated by encoding restriction endonuclease sites in the underlined regions, which allows the direct incorporation of randomized oligonucleotides at these positions
- a preferred embodiment generates a Xhol site at the double underlined LE site and a Hmdlll site at the double- underlined KL site
- the presentation structure is a mmibody structure
- a "minibody” is essentially composed of a minimal antibody complementarity region
- a preferred mmibody presentation structure is as follows
- the presentation structure is a sequence that contains generally two cyste e residues, such that a disulfide bond may be formed, resulting in a conformationally constrained sequence
- This embodiment is particularly preferred when secretory targeting sequences are used
- any number of random sequences, with or without spacer or linking sequences, may be flanked with cysteme residues
- effective presentation structures may be generated by the random regions themselves
- the random regions may be "doped" with cysteme residues which, under the appropriate redox conditions, may result in highly cross nked structured conformations, similar to a presentation structure
- the randomization regions may be controlled to contain a certain number of residues to confer ⁇ -sheet or ⁇ -hehcal structures
- the fusion partner is a targeting sequence that targets the candidate bioactive agent to a particular subcellular location
- the localization of proteins within a cell is a simple method for increasing effective concentration and determining function The concentration of a protein can also be simply increased by nature of the localization Shuttling the proteins into the nucleus confines them to a smaller space thereby increasing concentration
- targeting sequences such as targeting sequences to the Golgi endoplasmic reticulum, nuclear membrane, mitochondria, secretory vesicles, lysosome, and cellular membrane may be used, a preferred embodiment uses targeting sequences to the nucleus, i e a nuclear localization signal (NLS)
- the targeting sequence is a nuclear localization signal (NLS)
- NLSs are generally short, positively charged (basic) domains that serve to direct the entire protein in which they occur to the cell's nucleus
- Numerous NLS ammo acid sequences have been reported including single basic NLS's such as that of the SV40 (monkey virus) large T Antigen (Pro Lys Lys Lys Arg Lys Val), Kalderon (1984), et al , Cell, 39 499-509, the human retmoic acid receptor- ⁇ nuclear localization signal
- the fusion partner is a rescue sequence
- a rescue sequence is a sequence which may be used to purify or isolate either the candidate agent or the nucleic acid encoding it
- peptide rescue sequences include purification sequences such as the H ⁇ s 6 tag for use with Ni affinity columns and epitope tags for detection, immunoprecipitation or FACS
- Suitable epitope tags include myc (for use with the commercially available 9E10 antibody), the BSP biotmylation target sequence of the bacterial enzyme BirA, flu tags, lacZ, and GST
- the rescue sequence may be a unique oligonucleotide sequence which serves as a probe target site to allow the quick and easy isolation of the retroviral construct, via PCR, related techniques, or hybridization
- the fusion partner is a stability sequence to confer stability to the candidate bioactive agent or the nucleic acid encoding it
- peptides may be stabilized by the incorporation of glycmes after the initiation methionine (MG or MGG0), for protection of the peptide to ubiquitmation as per Varshavsky's N-End Rule, thus conferring long half-life in the cytoplasm
- preferred stability sequences are as follows MG(X) n GGPP, where X is any ammo acid and n is an integer of at least four
- the fusion partner is a dime ⁇ zation sequence
- a dimerization sequence allows the non-covalent association of one random peptide to another random peptide, with sufficient affinity to remain associated under normal physiological conditions This effectively allows small libraries of random peptides (for example, 10 4 ) to become large libraries if two peptides per cell are generated which then dime ⁇ ze, to form an effective library of 10 8 (10 4 X 10 4 ) It also allows the formation of longer random peptides, if needed, or more structurally complex random peptide molecules
- the dimers may be homo- or heterodimers
- Dimerization sequences may be a single sequence that self-aggregates, or two sequences, each of which is generated in a different retroviral construct That is, nucleic acids encoding both a first random peptide with dimerization sequence 1 , and a second random peptide with dimerization sequence 2, such that upon introduction into a cell and expression of the nucleic acid, dimerization sequence 1 associates with dimerization
- dimerization sequences will encompass a wide variety of sequences Any number of protein- protein interaction sites are known. dimerization sequences may also be elucidated using standard methods such as the yeast two hybrid system, traditional biochemical affinity binding studies, or even using the present methods
- the fusion partner is a detection gene, preferably a labeling gene or a survival gene That is, it is desirable to know that the candidate bioactive agent is a) present and b) being expressed
- preferred embodiments utilize fusion constructs utilizing genes that allow the detection of cells that contain candidate bioactive agents, as is generally outlined in the Examples, and shown in Figure 10
- Preferred detection genes include, but are not limited to, GFP, BFP, YFP, RFP, luciferase, and ⁇ -galactosidase
- Preferred embodiments utilize detection genes that are different from the reporter genes used to determine whether the IL-4 inducible promoter is inhibited, that is, if a GFP reporter gene is used, preferably a non-GFP detection gene is used This allows cell enrichment using FACS that can distinguish between cells containing candidate agents and those that do not, as well distinguishing cells containing candidate agents that do not inhibit the promoter and cells containing candidate agents that do inhibit the promoter
- nucleic acid encoding a peptide candidate agent which may also include other fusion partners as described herein
- the two nucleic acids are fused together in such a way as to only require a single promoter, i e using either an IRES site or a protease cleavage site such as 2a
- a single promoter i e using either an IRES site or a protease cleavage site such as 2a
- the fusion partners may be placed anywhere (i e N-termmal, C-termmal, internal) in the structure as the biology and activity permits
- the fusion partner includes a linker or tethering sequence, as generally described in PCT US 97/01019, that can allow the candidate agents to interact with potential targets unhindered
- useful linkers include glycine-se ⁇ ne polymers (including, for example, (GS) n , (GSGGS) n and (GGGS) n , where n is an integer of at least one), glycme-alanine polymers, alanine-se ⁇ ne polymers, and other flexible linkers such as the tether for the shaker potassium channel, and a large variety of other flexible linkers, as will be appreciated by those in the art Glycine-se ⁇ ne polymers are preferred since both of these am o acids are relatively unstructured, and therefore may be able to serve as a neutral tether between components Secondly, se ⁇ ne is hydrophilic and therefore able to solubihze what could be a
- the fusion partners may be modified, randomized, and/or matured to alter the presentation orientation of the randomized expression product
- determinants at the base of the loop may be modified to slightly modify the internal loop peptide tertiary structure, which maintaining the randomized ammo acid sequence
- combinations of fusion partners are used
- any number of combinations of presentation structures, targeting sequences, rescue sequences, and stability sequences may be used, with or without linker sequences
- candidate agents can include these components, and may then be used to generate a library of fragments, each containing a different random nucleotide sequence that may encode a different peptide
- the ligation products are then transformed into bacteria, such as E coli, and DNA is prepared from the resulting library, as is generally outlined in Kitamura, PNAS USA 92 9146-9150 (1995), hereby expressly incorporated by reference
- Suitable retroviral packaging system cell lines include, but are not limited to, the Bing and BOSC23 cell lines described in WO 94/19478, Soneoka et al , Nucleic Acid Res 23(4) 628 (1995), Finer et al , Blood 83 43 (1994), Pheonix packaging lines such as PhiNX-eco and PhiNX-ampho, described below, 292T + gag-pol and retrovirus envelope, PA317, and cell lines outlined in Markowitz et al , Virology 167 400 (1988), Markowitz et ai , J Virol 62 1120 (1988), Li et al , PNAS USA 93 11658 (1996), Kmsella et al , Human Gene Therapy 7 1405 (1996), all of which are incorporated by reference
- Preferred systems include PhiNX-eco and PhiNX-ampho or similar cell lines, disclosed in PCT US97/01019
- the candidate agents are added to the cells under reaction conditions that favor agent- target interactions Generally, this will be physiological conditions Incubations may be performed at any temperature which facilitates optimal activity, typically between 4 and 40°C Incubation periods are selected for optimum activity, but may also be optimized to facilitate rapid high through put screening Typically between 0 1 and 1 hour will be sufficient Excess reagent is generally removed or washed away
- reagents may be included in the assays These include reagents like salts, neutral proteins, e g albumin, detergents, etc which may be used to facilitate optimal protein-protein binding and/or reduce non-specific or background interactions Also reagents that otherwise improve the efficiency of the assay, such as protease inhibitors, nuclease inhibitors, anti-microbial agents, etc , may be used The mixture of components may be added in any order that provides for the requisite binding
- the IL-4 inducible e promoter is induced Alternatively, the promoter is induced prior to the addition of the candidate bioactive agents, or simultaneously This is generally done as is known in the art, and involves the addition of IL-4 or IL-13 to the cells at a concentration of not less than 5 units/ml with 200 units/ml being most preferred Addition of IL-4 or IL-13 is usually 24-48 hours after the bioactive agents are added
- the presence or absence of the reporter gene is then detected This may be done in a number of ways, as will be appreciated by those in the art, and will depend in part on the reporter gene For example, cells expressing a label reporter gene, such as GFP, can be distinguished from those not expressing the gene, and preferably sorted (enriched by FACS) on this basis Similarly, cells expressing the death gene will die, leaving only cells that have inhibited promotion of the expression of the gene, etc In general, the cells that express the reporter gene (i e non-inhibited IL-4 inducible e promoter) and separated from those that do not (i e the IL-4 inducible ⁇ promoter was inhibited) This may be done using FACS, lysis selection using complements, cell cloning, scanning by a Fluo ⁇ mager, growth under drug resistance, enhanced growth, etc
- sorting of cells containing bioactive agents that inhibit the IL-4 inducible ⁇ promoter (and thus do not turn on the death gene) from those cells that contain candidate agents that do not inhibit the promoter is simple only those surviving cells contain such an agent
- the presence or absence of the reporter gene is determined using a fluorescent-activated cell sorter (FACS)
- FACS fluorescent-activated cell sorter
- the expression of the reporter gene comprising a label is optimized to allow for efficient enrichment by FACS
- 10 to 1000 fluores per sorting event are needed, i e per cell, with from about 100 to 1000 being preferred, and from 500 to 1000 being especially preferred
- the cells are sorted at very high speeds, for example greater than about 5,000 sorting events per sec, with greater than about 10,000 sorting events per sec being preferred, and greater than about 25,000 sorting events per second being particularly preferred, with speeds of greater than about 50,000 to 100,000 being especially preferred.
- very high speeds for example greater than about 5,000 sorting events per sec, with greater than about 10,000 sorting events per sec being preferred, and greater than about 25,000 sorting events per second being particularly preferred, with speeds of greater than about 50,000 to 100,000 being especially preferred.
- the sorting results in a population of cells containing the reporter protein (i e the promoter was not inhibited) and at least one population of cells without the reporter protein (i e the promoter was inhibited)
- the absence of the reporter protein is indicative that at least one candidate bioactive agent is a bioactive agent that inhibits the IL-4 inducible ⁇ promoter
- the invention also provides methods for screening candidate agents for the ability to modulate IgE production
- modulating IgE production herein is meant either an increase or a decrease in IgE production, as quantified by the amount of IgE protein made
- cells that have already switched to the ⁇ heavy chain region can no longer be blocked at the earlier phase of IgE production This is especially important for memory B cells that maintain their capacity to secrete IgE and are long lived
- candidate agents are screened to identify compounds that can block IgE at the level of ⁇ heavy chain transcription, translation, assembly and trafficking, to prevent the terminal stages of IgE production
- a candidate bioactive agent is combined with a cell capable of expressing IgE, preferably surface IgE Preferred cells include, but are not limited to, cells that produce surface IgE such as the U266 cell line (Lagging, et al , "Distribution of Plasma Cell Markers and Intracellular IgE in Cell
- the candidate agent and the cells are combined, as outlined above, and the cells screened for alterations in the amount of IgE produced, as compared to the amount produced in the absence of the candidate bioactive agent
- This may be done using standard IgE labeling techniques, including, but not limited to, the use of anti-lgE antibodies, that may be either directly or indirectly labeled, for example through the use of fluorescent anti-lgE antibodies or fluorescent secondary antibodies, and through the use of IgE fusion proteins, as outlined below
- the amount of IgE produced is determined through the use of IgE fusion proteins, that is, the IgE is produced as a fusion protein comprising the IgE protein, specifically at least the e heavy chain, and a detectable protein such as is generally outlined above for label reporter genes
- gene "knock in" cell lines are produced, as outlined above and shown in the Figures
- a first label gene such as the gene for green fluorescent protein (GFP)
- GFP green fluorescent protein
- BFP blue fluorescent protein
- Suitable label genes for this embodiment include, but are not limited to, GFP, BFP, YFP and RFP
- the present invention provides cell lines that produce fusion proteins comprising IgE (either secreted or membrane bound) fused to a label protein, preferably a fluorescent protein
- the invention provides methods of identifying proteins that bind to all or part of the switch ⁇ region ( Figure 2B)
- the general idea is to use a "one hybrid" system to identify proteins that bind to all or part of the switch ⁇ region
- the present invention provides compositions comprising a test vector and a reporter vector, and cells containing these vectors These cells may be yeast, such as YM4271 or any yeast cell lines that reporter constructs can be inserted into
- vector or "episome” herein is meant a rephcon used for the transformation of host cells
- the vectors may be either self-rephcating extrachromosomal vectors ("plasmids") or vectors which integrate into a host genome
- plasmids extrachromosomal vectors
- a preferred embodiment utilizes retroviral vectors, as is more fully described below
- Suitable vectors will depend on the host cells used For use ot the system in yeast, suitable vectors are known in the art and include, but are not limited to, pH ⁇ s ⁇ -1 and pLacZi (Clonetech Cat #K1603-1 ) (Li, et al , "Isolation of ORC6, A Component of the Yeast Origin of Recognition Complex By a One- Hybrid System," Science 262 1870-1873 (1993), Liu, et al "Identifying DNA-Binding Sites and Analyzing DNA-Binding Domains Using a Yeast Selection System," In Methods A Companion to
- Preferred promoter sequences for expression in yeast include the inducible GAL1 ,10 promoter, the promoters from alcohol dehydrogenase enolase, glucokmase, glucose-6-phosphate isomerase, glyceraldehyde- 3-phosphate-dehydrogenase hexokmase, phosphofructokmase, 3-phosphoglycerate mutase, pyruvate kinase, and the acid phosphatase gene
- Yeast selectable markers include ADE2, HIS4, LEU2, TRP1 , and ALG7, which confers resistance to tunicamycin, the neomycin phosphotransferase gene, which confers resistance
- suitable vectors are derived from any number of known vectors, including, but not limited to, pCEP4 (Invitrogen), pCI-NEO (Promega), and pBI-EGFP (Clontech) Basically, any mammalian expression vectors with strong promoters such as CMV can be used to construct test vectors
- one or more retroviral vectors are used Currently, the most efficient gene transfer methodologies harness the capacity of engineered viruses, such as retroviruses, to bypass natural cellular barriers to exogenous nucleic acid uptake
- the use of recombinant retroviruses was pioneered by Richard Mulligan and David Baltimore with the Ps ⁇ -2 lines and analogous retrovirus packaging systems, based on NIH 3T3 cells (see Mann et al , Cell 33 153-159 (1993), hereby incorporated by reference)
- helper-defective packaging lines are capable of producing all the necessary trans proteins -gag, pol, and env- that are required for packaging, processing, reverse transcription, and integration of recombinant genomes Those RNA molecules that have in cis the ⁇ packaging signal are packaged into maturing vi ⁇ ons
- Retroviruses are preferred for a number of reasons First, their derivation is easy Second, unlike
- retrovirus-mediated gene delivery expression from retroviruses is long-term (adenoviruses do not integrate)
- Adeno-associated viruses have limited space for genes and regulatory units and there is some controversy as to their ability to integrate Retroviruses therefore offer the best current compromise in terms of long-term expression, genomic flexibility, and stable integration, among other features
- the mam advantage of retroviruses is that their integration into the host genome allows for their stable transmission through cell division This ensures that in cell types which undergo multiple independent maturation steps, such as hematopoietic cell progression, the retrovirus construct will remain resident and continue to express
- transfection efficiencies can be extremely high, thus obviating the need for selection genes in some cases
- retroviral vectors include a vector based on the murine stem cell virus (MSCV) (see Hawley et al , Gene Therapy 1 136 (1994)) and a modified MFG virus (Rivere et al , Genetics 92 6733 (1995)), and pBABE (see PCT US97/01019, incorporated by reference) Particularly preferred vectors are shown in Figure 11
- the retroviral vectors may include inducible and constitutive promoters Constitutive promoters are preferred for the bait and test vectors, and include, but are not limited to, CMV, SV40 Sr ⁇ , RSV, and TK Similarly, the reporter vector promoter is associated with at least one copy of an operator, as outlined herein
- Tet-mducible retroviruses can be used to express bait or test genes (Hoffman et ai , PNAS USA 93 5185 (1996)) Expression of this vector in cells is virtually undetectable in the presence of tetracychne or other active analogs
- Tet in the absence of Tet, expression is turned on to maximum within 48 hours after induction, with uniform increased expression of the whole population of cells that harbor the inducible retrovirus, indicating that expression is regulated uniformly within the infected cell population
- a similar, related system uses a mutated Tet DNA-bmdmg domain such that it bound DNA in the presence of Tet, and was removed in the absence of Tet Either of these systems is suitable
- these expression vectors include transcriptional and translational regulatory nucleic acid operably linked to nucleic acids which are to be expressed "Operably linked” in this context means that the transcriptional and translational regulatory nucleic acid is positioned relative to any coding sequences in such a manner that transcription is initiated Generally, this will mean that the promoter and transcriptional initiation or start sequences are positioned 5' to the coding region
- the transcriptional and translational regulatory nucleic acid will generally be appropriate to the host cell used, as will be appreciated by those in the art Numerous types of appropriate expression vectors, and suitable regulatory sequences, are known in the art for a variety of host cells
- the transcriptional and translational regulatory sequences may include, but are not limited to, promoter sequences, ribosomal binding sites, transcriptional start and stop sequences, translational start and stop sequences, and enhancer or activator sequences
- the regulatory sequences include a promoter and transcriptional start and stop sequences
- Promoter sequences encode either constitutive or inducible promoters
- the promoters may be either naturally occurring promoters hybrid or synthetic promoters Hybrid promoters, which combine elements of more than one promoter, are also known in the art, and are useful in the present invention
- the vectors of the present invention utilize two different types of promoters
- the promoters on the bait and test vectors are constitutive, and drive the expression of the fusion proteins and selection genes, if applicable, at a high level
- the test vector comprises a selection gene Selection genes allow the selection of transformed host cells containing the vector, and particularly in the case of mammalian cells, ensures the stability of the vector, since cells which do not contain the vector will generally die Selection genes are well known in the art and will vary with the host cell used Suitable selection genes include, but are not limited to, neomycin blastocidm, bleomycm, puromycin, hygromycin, and other drug resistance genes, as well as genes required for growth on certain media, including, but not limited to, His and Lev or His and Trp In some cases, for example when using retroviral vectors, the requirement for selection genes is lessened due to the high transformation efficiencies which can be achieved Accordingly, selection genes need not be used in retroviral constructs, although they can be In addition, when retroviral vectors are used, the test vectors may also contain detectable genes as are described herein rather than selection genes, it may be desirable to verify that the vector is present in the cell, but not require selective pressure for maintenance
- the test vector comprises a fusion gene comprising a first sequence encoding a transcriptional activation domain, and a second sequence encoding a test protein
- fusion gene or “fusion construct” herein is meant nucleic acid that comprises at least two functionally distinct sequences, i e generally sequences from two different genes
- sequences described herein may be DNA, for example when extrachromosomal plasmids are the vectors, or RNA, for example when retroviral vectors are used
- the sequences are directly linked together without any linking sequences, although in some embodiments linkers such as restriction endonuclease cloning sites or linkers encoding flexible ammo acids such as glycine and se ⁇ ne linkers such as are known in the art are used
- the first fusion gene comprises a first sequence encoding a transcriptional activation domain
- transcriptional activator domain herein is meant a protemaceous domain which is able to activate transcription
- Suitable transcription activator domains include, but are not limited to, transcriptional activator domains from GAL4 (ammo acids 1-147, see Fields et al , Nature 340 245 (1989), and Gill et al , PNAS USA 87 2127 (1990)), GCN4 (from S cerevisiae, Hope et al , Cell 46 885 (1986)), ARD1 (from S cerevisiae, Thukral et al , Mol Cell Biol 9 2360 (1989)), the human estrogen receptor (Kumar et al , Cell 51 941 (1987)), VP16 (T ⁇ ezenberg et al , Genes Dev 2(6) 718-729 (1988)), and B42 (Gyuris et al, Cell 1993), and NF-kB p65 and derivatives thereof which are functionally similar
- test protein herein is meant a candidate protein which is to be tested for interaction with a bait protein
- Protein in this context means proteins ohgopeptides, and peptides, i e at least two ammo acids attached
- the test protein sequence is one of a library of test protein sequences, that is, a library of test proteins is tested for binding to one or more bait proteins
- the test protein sequences can be derived from genomic DNA, cDNA or can be random sequences Alternatively, specific classes of test proteins may be tested
- the library of test proteins or sequences encoding test proteins are incorporated into a library of test vectors, each or most containing a different test protein sequence
- test protein sequences are derived from genomic DNA sequences Generally, as will be appreciated by those in the art, genomic digests are cloned into test vectors
- the genomic library may be a complete library, or it may be fractionated or enriched as will be appreciated by those in the art
- test protein sequences are derived from cDNA libraries
- a cDNA library from any number of different cells may be used, and cloned into test vectors
- the cDNA library may be a complete library, or it may be fractionated or enriched in a number of ways
- test protein sequences are random sequences Generally, these will be generated from chemically synthesized oligonucleotides Generally, random test proteins range in size from about 2 ammo acids to about 100 ammo acids, with from about 10 to about 50 ammo acids being preferred Fully random or "biased" random proteins may be used, that is, some positions within the sequence are either held constant or are selected from a limited number of possibilities
- the nudeotides or ammo acid residues are randomized within a defined class, for example, of hydrophobic ammo acids, hydrophilic residues, ste ⁇ cally biased (either small or large) residues, towards the creation of cystemes, for cross-linking, prohnes for SH-3 domains, sermes, threonmes tyros es or histid es for phosphorylation sites, etc , for zinc fingers, SH-2 domains, stem loop structures, or to pu ⁇ nes, or to reduce the chance of creation
- compositions are introduced into host cells to screen for protein- nucleic acid interactions
- introduction into or grammatical equivalents herein is meant that the nucleic acids enter the cells in a manner suitable for subsequent expression of the nucleic acid
- the method of introduction is largely dictated by the targeted cell type and the composition of the vector Exemplary methods include CaP0 4 precipitation, hposome fusion, hpofectin®, electroporation, viral infection, etc
- the vectors may stably integrate into the genome of the host cell (for example, with retroviral introduction for mammalian cells, outlined herein), or may exist either transiently or stably in the cytoplasm (i e through the use of traditional plasmids, utilizing standard regulatory sequences, selection markers, etc )
- the vectors can be introduced simultaneously, or sequentially in any order
- host cells containing the reporter construct are generated first, and preferably the reporter vector is integrated into the genome of the host cell, for example, using a retroviral reporter vector
- the cell is subjected to conditions under which the selectable markers and fusion proteins are expressed If a test protein has sufficient affinity to the switch ⁇ region to activate transcription, the detectable protein is produced, and cells containing these proteins will survive drug selection and can be detected as outlined above
- the detectable protein will be produced at a measurably higher level than in the absence of a protem- nucleic acid interaction
- the determination of a protein-nucleic acid interaction is generally done on the basis of the presence or absence of the detectable gene(s)
- the cell is isolated from the plurality which do not have altered phenotypes
- Suitable isolation techniques include, but are not limited to, drug selection, FACS, lysis selection using complement, cell cloning, scanning by Fluo ⁇ mager, expression of a "survival" protein, induced expression of a cell surface protein or other molecule that can be rendered fluorescent or taggable for physical isolation, expression of an enzyme that changes a non-fluorescent molecule to a fluoroscent one, overgrowth against a background of no or slow growth, death of cells and isolation of DNA or other cell vitality indicator dyes, changes in fluorescent characteristics, etc
- the preferred isolation techniques are drug selection and FACS based on the expression of the detectable gene, with a preferred embodiment utilizing both simultaneously
- test protein nucleic acid and/or the test protein is isolated from the positive cell This may be done in a number of ways
- primers complementary to DNA regions common to the vector, or to specific components of the library such as a rescue sequence are used to "rescue" the unique test sequence
- the test protein is isolated using a rescue sequence
- rescue sequences comprising epitope tags or purification sequences may be used to pull out the test protein, using immunoprecipitation or affinity columns
- the test protein may be detected using mass spectroscopy
- the chactenzation of the bioactive agent is done This will proceed as will be appreciated by those in the art, and generally includes an analysis of the structure, identity, binding affinity and function of the agent Depending on the type of agent, this may proceed in a number of ways
- the candidate nucleic acid and/or the bioactive agent is isolated from the cells This may be done in a number of ways
- primers complementary to DNA regions common to the retroviral constructs, or to specific components of the library such as a rescue sequence, defined above are used to "rescue" the unique random sequence
- the bioactive agent is isolated using a rescue sequence
- rescue sequences comprising epitope tags or purification sequences may be used to pull out the bioactive agent, using immunoprecipitation or affinity columns
- the peptide may be detected using mass spectrometry
- candidate agents from other chemical classes can be identified and characterized, for example through the use of mass spectroscopy This information can then be used in a number of ways
- the bioactive agent is resynthesized and remtroduced into the target cells, to verify the effect
- This may be done using retroviruses, or alternatively using fusions to the HIV-1 Tat protein, and analogs and related proteins, which allows very high uptake into target cells See for example, Fawell et al , PNAS USA 91 664 (1994), Frankel et al , Cell 55 1189 (1988), Savion et al , J Biol Chem 256 1149 (1981) Derossi et al , J Biol Chem 269 10444 (1994), and Baldin et ai , EMBO J 9 1511 (1990), all of which are incorporated by reference
- Other techniques known in the art may be used as well
- the sequence of a bioactive agent is used to generate more candidate bioactive agents
- the sequence of the bioactive agent may be the basis of a second round of (biased) randomization, to develop bioactive agents with increased or altered activities
- the second round of randomization may change the affinity of the bioactive agent
- the bioactive agent may be formulated
- the compounds having the desired pharmacological activity may be administered in a physiologically acceptable carrier to a host, as previously described
- the agents may be administered in a variety of ways, orally, parenterally e g , subcutaneously, intraperitoneally, mtravascularly, etc Depending upon the manner of introduction, the compounds may be formulated in a variety of ways
- the concentration of therapeutically active compound in the formulation may vary from about 0 1-100 wt %
- compositions can be prepared in various forms, such as granules, tablets, pills, suppositories, capsules, suspensions, salves, lotions and the like
- Pharmaceutical grade organic or inorganic carriers and/or diluents suitable for oral and topical use can be used to make up compositions containing the therapeutically-active compounds
- Diluents known to the art include aqueous media, vegetable and animal oils and fats Stabilizing agents, wetting and emulsifying agents, salts for varying the osmotic pressure or buffers for securing an adequate pH value, and skin penetration enhancers can be used as auxiliary agents
- both GFP and BFP constructs will be transfected and cells sorted for expression of both reporters
- a candidate bioactive agent library in this case a peptide library, will be packaged into infectious viral particles as outlined below
- a preferred library is a mixture of random peptide sequences with and without a nuclear localization sequence (NLS) upstream of a reporter gene to identify infected ceils and relative peptide expression (see Figure 6)
- NLS nuclear localization sequence
- Each screen will start with production of the primary retrovirus peptide library, as is generally shown in Figure 7 This is generally done as outlined in PCT US97/01019 and PCT US97/01048, both of which are expressly incorporated by reference In general, this is done as follows
- the Phoenix cells are seeded in 10 cm plates at 5 X 106 cells in 6 ml (DMEM + 10% FBS + Pen/Strep) per plate the day before transfection Day 2 allow all reagents to reach room temperature 30 m before starting
- This primary library will be used to infect at least 10 9 knock-in cells After infection, the cells will be stimulated with IL-4 and two days later, peptide-containmg cells (identified by the fluorescent reporter) that are negative for the knock-in reporter (i e where there is ⁇ promoter inhibition) will be sorted by
- FACS This enriched, knock-in reporter negative population will be subjected to RT-PCR to amplify the integrated peptide sequences
- the PCR material will be used to construct a new "enriched" retrovirus peptide library to initiate the next screening round
- V R ⁇ ⁇ 1 + e ) p ) +v
- R ratio of true positive cells over the total number of cells screened per round of selection
- L frequency of true positive cells (ie # of cells expressing peptide inhibitors of IgE switch/synthesis)
- g frequency of non-heritable false-positive cells (ie # of cells in which IgE switch/synthesis is inhibited due to stimulation/screening inefficiencies, but are IgE positive in subsequent selection rounds)
- D number of rounds of selection/enrichment applied to library screen
- Q initial frequency of cells with an heritable false-positive phenotype (ie dominant- negative somatic mutation in cells that prevent IgE switch/synthesis)
- $ frequency of false-positives incurred by or during the selection/enrichment process
- a conservative estimate of IgE inhibitory peptides in the starting population is 1/10 8 , therefore v -10 8 Solving the equation for the number of selection rounds required to enrich to 50% true positive hits 10
- the most important factor that influences the number of enrichment rounds necessary to identify individual peptide hits is the ratio between the real positive peptide hits in the original library and the heritable false positives
- the frequency of real positive peptide hits is dependent upon the qualitative ability of the peptide to access and, in the correct conformation, bind to regulatory domains on proteins in the pathway of interest
- multiple scaffolding structures are used for presentation of random peptide surfaces and also different localization sequences fused to those peptide structures Enrichment of real positive peptides becomes less efficient with false positive rates above 2% For this reason, great emphasis is placed on developing robust reporter constructs and cell lines
- RT-PCR amplification may decrease overall amplification of real peptides hits from one round to another This is overcome by additional rounds of library enrichment and is why RT-PCR amplification is carefully monitored after each round of screening
- IgE transcription, translation, assembly and trafficking may be identified
- the lgE + cell line, U266, expresses IgE on the surface and also secretes IgE Antibodies against surface IgE heavy and light chains have been obtained and both are used to fluorescently mark IgE positive cells
- the U266 line is consistently greater than 98 5% positive for membrane IgE
- the construct will contain an SV40 promoter-driven neomycin resistance gene which is flanked by loxP sites and inserted in the intron between the CH3 and CH4 exons ( Figure 8)
- TK gene will be cloned 3' of the longer homologous sequence region U266 cells transfected with this construct will be selected for integration by cultunng them in the presence of G418
- the surviving cells will be cultured in ganciclovir to select against cells containing the HSV-TK gene (the HSV-TK gene at the 3' end will be deleted during the desired homologous recombination event)
- the remaining clones will be assessed for homologous recombination by PCR Clones containing homologously-recombined constructs will be transfected with ere to mediate excision of the SV40 promoter/neomycm resistance gene in order to eliminate promoter interference Excision will be verified by subdividing clones into parent and daughter pools and re-selecting the latter pool in G418
- the parental cells corresponding to G418 sensitive daughter cells will be subdivided again and tested for GFP and BFP expression
- Parental stocks of the most inducible clones
- Inhibitors are predicted to interfere with IL-4/13 signaling as well as nuclear transcription of the e germhne gene
- Three lgM + , EBV human B cells lines (CA-46, MC116, and DND39, see Figure 4) that produce e germhne transcripts in the presence of IL-4 will be infected with the following construct a retroviral vector containing an IL-4 responsive 600 bp fragment of the e promoter in the reverse orientation followed by a splice site, GFP encoding sequence and a poly-adenylation sequence ( Figure 10) Briefly, cells will be infected with the reporter construct and induced with IL-4 The cells will then be sorted by FACS for GFP reporter expression The IL-4 will be removed and the cells will be sorted for the absence of reporter fluorescence From these sorts, several clones will be established that turn on the reporter in the presence of IL-4, indicating activation of the germhne e promoter
- Example 5 Screening of candidate agents using reporter cell line
- the cell line of Example 4 is infected infected with a peptide library as described above
- the peptide library is packaged into infectious viral particles (see Figure 7)
- the library is a mixture of random peptide sequences with and without a nuclear localization sequence (NLS) upstream of a reporter gene to identify infected cells and relative peptide expression ( Figure 6)
- Each screen will start with production of the primary retrovirus peptide library
- This primary library will be used to infect at least 10 9 ⁇ promoter reporter cells After infection, the cells will be stimulated with IL-4 and two days later, the FACS will sort peptide-contaming, reporter negative cells (i e where there is e promoter inhibition)
- This enriched, reporter negative population will be subjected to RT-PCR to amplify the integrated peptide sequences
- the PCR material will be used to construct a new "enriched" retrovirus peptide library to initiate the next screening round
- the retroviral construct consists of the 600 bp IL-4 inducible e promoter downstream of a self-inactivating (SIN) LTR, followed by a chimeric FAS receptor (FASr), the self- cleaving peptide 2a and, lastly, the drug-selectable puromycin resistance gene
- the chimeric receptor is composed of the mouse FASr external domain and the human FASr transmembrane and cytoplasmic domains
- a mouse specific anti-FASr antibody can be used which will bind only activated FASr produced by the survival construct
- the 2a self-cleaving peptide allows equimolar amounts of the chimeric FASr and puromycin to be produced in the cell
- IL-4 stimulation has been reported to dimmish FAS-mduced apoptosis in certain B-cell lines
- common suicide genes including Herpes Simplex Virus Thymidine Kinase (HSV-TK) or human cytochrome P450 2B1 in conjunction with ganciclovir or cyclophosphamide treatment respectively, can replace FASr-mediated death ( Figure 10)
- cell cycle arrest genes such as p21 can be used in place of toxic gene products (Figure
- the lgM + B cell lines described in Example 6 are infected with the survival construct Leaky cells (constitutive expression of the e promoter) will be removed by incubation with the anti-mouse FASr antibody Next, the cells are incubated in the presence of the inducer, IL-4, and the drug selection compound, puromycin Cells that contain a construct that is inducible by IL-4 will be resistant and survive This produces a population with an exogenous e promoter that is IL-4 inducible
- the peptide library is introduced into these cells and two days later they are induced with IL-4 in the presence of anti-mouse FASr monoclonal antibody Cells carrying peptides that inhibit induction of the engineered e promoter fragment will not produce the chimeric FASr and will survive After the survivors grow out (approximately 1 week), they will again be subjected to IL-4 and the anti-FASr treatment
- Example 8 One-hybrid screens for identification of proteins that bind to switch ⁇ region
- Recombmase proteins that bind to the S ⁇ region mediate the DNA rearrangement that generates a functional e heavy chain They may be specific for ⁇ switching cells or may bind to other proteins that target them specifically to the Se region Breakpoints in the recombination of the switch e region to the switch ⁇ region occur in a limited area of the switch e region Two stretches of the switch e region spanning the majo ⁇ ty of breakpoints will be used as bait in a one-hybrid screen ( Figure 2b)
- the cDNA libraries to be used are derived from the IgE positive cell line U266 (the assumption here is that the U266 line still contains the switch recombmase, certainly, the recombmase is turned off in plasma cells) and from human peripheral blood lymphocytes stimulated in vitro to switch with a high frequency to IgE
- the plgE-HIS was linearized at an Afl II site and integrated into yeast strain YM4271 (MATa, ura3-52, h ⁇ s3-200, ade2-101 , Iys2-801 , leu2-3, 112, trp1-901 , tyr1-501 , gal4- ⁇ 512, gal80- ⁇ 538, ade5 hisG) to construct the first yeast reporter strain YlgE-HIS SD-H plates were used to select for integrated reporters The yeast strain YlgE-HIS was tested on SD-H+3AT plates to determine the optimal concentration of 3AT to suppress basal level HIS expression from the minimal promoter
- the plgE-LacZ plasmid was linearized at an Nco I site and integrated into the yeast strain YlgE-HIS to construct a dual reporter strain YlgE-HL SD-U plates were used to select for cells with dual reporters integrated
- the dual reporter strain will be used for transformation by the U266 cDNA library (it is assumed that the U266 line still contains the switch recombmase) and the IgE switching PBL cDNA library At least 20 million transformants from each library will be screened on SD-LH+3AT plates Clones that can grow up and turn blue on SD-LH+3AT plates will be grown up in SD-L liquid medium for plasmid retrieval Retrieved cDNA clones will be further tested using in vitro binding assays
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US6455247B1 (en) * | 1996-01-23 | 2002-09-24 | Board Of Trustees Of The Leland Stanford Junior University | Methods for screening for transdominant effector peptides and RNA molecules |
US5804387A (en) * | 1996-02-01 | 1998-09-08 | The Board Of Trustees Of The Leland Stanford Junior University | FACS-optimized mutants of the green fluorescent protein (GFP) |
CA2283461A1 (en) | 1997-03-14 | 1998-09-17 | Uab Research Foundation | Adenoviral vectors with modified tropism |
US7001733B1 (en) | 1998-05-12 | 2006-02-21 | Rigel Pharmaceuticals, Inc. | Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement |
US6280937B1 (en) * | 1998-08-14 | 2001-08-28 | Rigel Pharmaceuticals, Inc. | Shuttle vectors |
US6645253B2 (en) * | 1999-06-03 | 2003-11-11 | Carl A. Caspers | Vacuum pump and shock absorber for artificial limb |
-
1998
- 1998-05-12 US US09/076,624 patent/US7001733B1/en not_active Expired - Lifetime
-
1999
- 1999-05-12 WO PCT/US1999/010497 patent/WO1999058663A1/en not_active Application Discontinuation
- 1999-05-12 AU AU39862/99A patent/AU765000C/en not_active Ceased
- 1999-05-12 JP JP2000548454A patent/JP2002514416A/en active Pending
- 1999-05-12 CA CA002328481A patent/CA2328481A1/en not_active Abandoned
- 1999-05-12 EP EP99922992A patent/EP1076695A1/en not_active Withdrawn
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2001
- 2001-09-25 US US09/963,206 patent/US7041467B2/en not_active Expired - Lifetime
- 2001-09-25 US US09/963,247 patent/US7153665B2/en not_active Expired - Lifetime
- 2001-09-27 US US09/966,976 patent/US6953688B2/en not_active Expired - Lifetime
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2002
- 2002-03-08 US US10/096,339 patent/US7056687B2/en not_active Expired - Lifetime
-
2006
- 2006-02-24 US US11/361,631 patent/US20060147978A1/en not_active Abandoned
- 2006-03-09 US US11/373,429 patent/US20060183163A1/en not_active Abandoned
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US20060183163A1 (en) | 2006-08-17 |
US7056687B2 (en) | 2006-06-06 |
WO1999058663A1 (en) | 1999-11-18 |
US7001733B1 (en) | 2006-02-21 |
US20020168649A1 (en) | 2002-11-14 |
US20060147978A1 (en) | 2006-07-06 |
US20020123076A1 (en) | 2002-09-05 |
AU765000B2 (en) | 2003-09-04 |
JP2002514416A (en) | 2002-05-21 |
US20030022196A1 (en) | 2003-01-30 |
EP1076695A1 (en) | 2001-02-21 |
CA2328481A1 (en) | 1999-11-18 |
US20040214155A1 (en) | 2004-10-28 |
US7041467B2 (en) | 2006-05-09 |
AU765000C (en) | 2004-06-17 |
US6953688B2 (en) | 2005-10-11 |
US7153665B2 (en) | 2006-12-26 |
AU3986299A (en) | 1999-11-29 |
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