WO1999033454A2 - S-procyclidine for treating urinary incontinence - Google Patents
S-procyclidine for treating urinary incontinence Download PDFInfo
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- WO1999033454A2 WO1999033454A2 PCT/US1998/027708 US9827708W WO9933454A2 WO 1999033454 A2 WO1999033454 A2 WO 1999033454A2 US 9827708 W US9827708 W US 9827708W WO 9933454 A2 WO9933454 A2 WO 9933454A2
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- pharmaceutical composition
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- procychdme
- pharmaceutically acceptable
- enantiomencally
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
Definitions
- the present invention relates to methods for treating urinary incontinence such as incontinence caused by bladder detrusor muscle instabilit ⁇ . and to pharmaceutical compositions for such treatment
- Urmar> mcontmence is a prevalent problem that affects people of all ages and levels of physical health, both in healthcare settings and in the community at large
- urinary incontinence afflicts 15-30% of elderlv people living at home, one-third of those living in acute-care settings, and at least one-half of those in long-term care institutions (R M Resmck. Lancet 346 94 ( 1995)) Medicalh . it predisposes persons to u ⁇ narv tract infections, pressure ulcers, pe ⁇ neal rashes, and urosepsis Psychosocial .
- urinary incontinence is associated with embarrassment, social st ⁇ gmat ⁇ .zat ⁇ on. depression.
- Treatments for mcontmence m include drugs with bladder relaxant properties. / e . which help to control bladder detrusor muscle overactrvrty Such drugs are effective in 80 to 85% of patients with uninhibited bladder contractions, with anticholmergic medications representmg the mamstay of this type of treatment
- anticholmergics such as propantheline bromide, m ⁇ combmation smooth muscle relaxant/anticholinergics such as racemic oxybutymn and dicyclomine, have been used to treat urge mcontmence (See, e g , A J Wein, Urol Chn N Am , 22.557-77 (1995) )
- racemic oxybutymn nevertheless is considered the drug of first choice in patients with bladder detrusor muscle hyperactivity when pharmacological therapy is indicated (cf Yarllur et al . Drugs Aging. 6 243 (1995))
- the present invention provides methods and compositions for treatment of urinary incontinence, including, e g , bladder detrusor muscle instability incontinence, stress mcontmence, urge mcontmence, overflow incontinence, enuresis, and post-prostectomy incontinence, with (5)-procychd ⁇ ne
- the methods of the present invention provide for treatment of incontinence with fewer adverse effects than occur upon administration of racemic procychdine
- One aspect of the present invention relates to methods for treating urinary mcontmence by admimstration to a subject m need thereof a therapeutically effective amount of enantiomerically ennched (S)-procychdme. or a pharmaceutically acceptable salt thereof
- the enantiomerically enriched (5)-procychd ⁇ ne, or a pharmaceutically acceptable salt thereof is substantially free of ( ⁇ )-procychdme
- the present invention also relates to methods for treating bladder detrusor muscle instability compnsmg administration to a subject in need thereof a therapeutically effective amount of enantiomencally ennched (Sj-procychdine, or a pharmaceutically acceptable salt thereof
- enantiomencally enriched (£)-procycl ⁇ d ⁇ ne. or a pharmaceutically acceptable salt thereof is substantially free of (R)-procycl ⁇ d ⁇ ne
- the phannaceutical compositions of the present invention comprise (,Sj-procychd ⁇ ne, or a pharmaceutically acceptable salt thereof, substantially free of (J.)-procycl ⁇ d ⁇ ne
- the present mvention also provides for formulating the pharmaceutical compositions of the present invention, compnsmg enantiomencally enriched or a pharmaceuticalh acceptable salt thereof, and a pharmaceutically acceptable carrier, m phaimaceutical unit dosage foims. including, e g , tablets and soft elastic gelatin capsules.
- a kit for treating u ⁇ na ⁇ , incontinence, such as bladder detrusor muscle instability incontinence, stress mcontmence is another embodiment of the present invention.
- kits compnses a pharmaceutical composition comprising enantiomencally enriched (S)-procychd ⁇ ne. or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable earner, and instructions for administenng the same while reducmg or eliminating anticholmergic adverse effects associated with administration of racemic procychdine, or other mcontmence drugs with anticholmergic action
- the enantiomencally enriched (S)-procychd ⁇ ne. or a pharmaceutically acceptable salt thereof is substantially free of (/?)-procychdme Detailed Descnption of the Invention
- urinary mcontmence can be caused by uncontrolled or unstable bladder contractions, particularly of the bladder detrusor muscle which serves to force fluids out of the bladder
- the major proportion of the neurohumeral stimulus for physiologic bladder contraction is acetylcholine-induced stimulation of postganghonic muscannic receptor sites on bladder smooth muscle Consistent with this obsei ⁇ ation.
- the present invention relates to compositions and methods for the treatment of bladder instability in mammals, such as humans More specifically, tins mvention provides enantiomencally ennched preparations of (S)-procychd ⁇ ne and methods for their use m the treatment of urinary incontinence, including, e g . bladder detrusor muscle instability mcontmence, stress mcontmence, urge mcontmence, overflow mcontmence.
- the present invention provides a method for treatmg urinary incontinence using (S)-procychdme, which results in a reduction of the adverse effects associated with admimstration of racemic procychdine
- the method compnses administering to a patient in need thereof a pharmaceutically effective amount of ( ⁇ S)-procychd ⁇ ne, or a pharmaceutically acceptable salt thereof, substantially free of ( ⁇ )-procychdme
- the methods of the present invention are used to treat urinary mcontmence due to bladder detrusor muscle instability Such instability may result in, for example, stress incontinence or urge incontinence, or combination thereof, and/or enuresis
- the present invention provides pharmaceutical compositions which compnse an enantiomencally enriched preparation of (S)-procychdme. or a pharmaceutically acceptable salt thereof, formulated together with one or more pharmaceutically acceptable earners (additives) and/or diluents
- the pharmaceutical compositions of the present invention may be specially formulated for admimstration in solid or liquid form, including those adapted for oral administration, parenteral administration, or topical application.
- chiral refers to molecules which have the property of non-supe ⁇ mposabilit ⁇ of the mirror image partner, while the term “achiral” refers to molecules which are supenmposable on their minor image partner
- S and R configuration are as defined by the IUPAC 1974 Recommendations for Section E , Fundamental Stereochemistry. Pure Appl Chem , 45 13-30 ( 1976) The teims racemate and enantiomer will be used m their normal context to descnbe the stereochemistry of procychdine preparations
- an enantiomencally enriched preparation of (S)-procychd ⁇ ne means a preparation of procychdine having greater than 50%) by weight of the (S)-procychdme enantiomer relative to the ( .)-procychd ⁇ ne enantiomer, more preferably at least 75% by
- substantially free of (/.)-procychdme will be understood to have similar purity ranges . i e , at least 85% by weight of the (S)-procychd ⁇ ne enantiomer relative to the ( ⁇ )-procychd ⁇ ne enantiomer, more preferably at least 90% by weight, and even more preferably at least 95% by weight
- adverse effects refers to effects associated with admimstration of racemic procychdine, which are not part of the desired therapeutic effect of the drug With respect to the treatment of unnary mcontmence, such adverse effects, include, for illustrative purposes, drowsmess, epistaxis, xerostomia, mydnasis.
- cycloplegia unstable cardiovascular status such as anhythnua (e g , tachycardia or palpitations), increased ocular pressure, nausea, constipation, decreased sweating, impotence, and/or dermal mamfestations such as urticana
- Epistaxis refers to nosebleeds, e g , hemorrhage from the nose Epistaxis is a side effect of anticholmergics in children
- xerostomia refers to dryness of the mouth due to lack of normal secretion
- mydnasis refers to dilation of the pupil, and often results in blurred vision
- cycloplegia refers to paralysis of the ciliary muscle, paralysis of accommodation
- enuresis refers to the involuntary discharge of urine
- nocturnal enuresis refers to involuntary discharge of unne during sleep at night
- enantiomers can be accomplished m several ways known in the art (See, e g , Hermanssen et al , J Chromat . 694 57-69 (1995)) For example, a racemic mixture of two enantiomers can be separated by chromatography using a chiral stationary phase (see, e g , "Chiral Liquid Chromatography”. W J Lough, Ed Chapman and Hall.
- Enantiomers can also be separated by classical resolution techniques For example, formation of diastereomet ⁇ c salts and fractional crystallization can be used to separate enantiomers
- the diastereomet ⁇ c salts can be formed by addition of enantiomencally pure chiral bases such as brucme, quinine, ephedrme, strychnine, and the like
- diastereomet ⁇ c esters can be formed with enantiomencally pure chiral alcohols such as menthol, followed by separation of the diastereomenc esters and hydrolysis to yield the free, enantiomencally enriched carboxyhc acid
- addition of chiral carboxyhc or sulfonic acids such as camphorsulfomc acid, tartanc acid, mandelic acid, or lactic acid can result m foimation of the
- the active enantiomer of procychdine can be synthesized by stereospecific synthesis to produce only the desired optical isomer usmg methodology well .known to those skilled in the art (See, e g., Sjo et al , Ada Chemica Scandinavia. 47 1019-1024 (1993), Schjekdenip et al .
- optical punty of the (S)-procychd ⁇ ne can be deteimined by methods known in the art
- a sample of the procychdine can be analyzed by high perfoimance liquid chromatography on a chiral chromatographic column
- Another method of determining optical purity involves making a chiral ester, such as a Mosher ester of a procychdine sample, and analyzing the NMR spectrum for the presence of the undesired enantiomer
- (S)-procychd ⁇ ne is substantially free of ( ⁇ )-procvchd ⁇ ne "Substantially free” as used herein, means that at least 85% by weight of the total procychdine present is the (.Sj-procychdine enantiomer, more preferably at least 90% by weight, and still more preferably at least 95% by weight is the (5)-procvchd ⁇ ne enantiomer In a more preferred embodiment, at least 99 % by weight of the total proc ⁇ chdine present is the (S)-procychdme enantiomer (5)-procycl ⁇ d ⁇ ne can be used to treat urinary incontinence, including, e g , bladder detrusor muscle instability incontinence, stress mcontmence, urge incontinence, overflow incontinence, enuresis, and post-prostectomy mcontmence, by administration to a patient according to any suitable route of administration (
- a preferred method of administration is oral administration .
- Another prefened route of administration is intravenous admimstration
- a particularly preferred route of admimstration is mtravesical delivery, i e , admimstration directly to the bladder, e g , by injection or infusion
- (S)-procychdme is preferably administered as a pharmaceutical fo ⁇ nulation (composition)
- pharmaceutically acceptable is employed herein to refer to those compounds, materials, compositions, and/or dosage forms winch are, within the scope of sound medical judgment, suitable for use m contact with the tissues of human bemgs and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/nsk ratio
- contains an ammo functional group and thus is capable of forming pharmaceutically acceptable salts
- pharmaceutically acceptable salts refers to the relatively non-toxic, inorganic and organic salts of ( «S)-procychd ⁇ ne
- Representative salts include the bromide, chlonde, hydrobromide, hydrochlonde.
- Formulations of the present mvention include those suitable for oral, nasal, topical (including buccal and sub ngual). rectal, vaginal and or parenteral administration
- the formulations may convemently be presented in unit dosage form and may be prepared by any methods well known m the art of pharmacy
- the amount of active ingredient which is combmed with a earner material to produce a single dosage form will vary depending upon the host being treated, and the particular mode of administration
- the amount of active ingredient which may be combined with a earner material to produce a smgle dosage form preferably will be that amount of (S)-procycl ⁇ d ⁇ ne which produces a therapeutic effect
- the amount of the active mgredient will range from about 1 % to about 99 % of the total formulation, preferably from about 5 % to about 70 %, and most preferably from about 10 % to about 30 %
- compositions or compositions include the step of bringing into association (5)-procychdme with a phaimaceutically acceptable earner and, optionally, one or more accessory mgredients
- the foimulations are prepared by unifo ⁇ nly and intimately bnngmg into association (»S)-procychd ⁇ ne with liquid earners, or finely divided solid carriers, or both, and any optional accessory ingredients, and then, if necessary, shaping the product
- pharmaceutically acceptable carrier means a pharmaceutically acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting the (S)-procychdme from one organ, or portion of the body, to another organ or portion of the body
- Each earner must be "acceptable” m the sense of being compatible with the other mgredients of the formulation and not injunous to the patient
- materials which can serve as pharmaceutically acceptable earners include ( 1 ) sugars, such as lactose, glucose and sucrose, (2) starches, such as corn starch and potato starch, (3) cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate, (4) powdered tragacanth, (5) malt. (6) gelatm, (7) talc, (8) excipients, such as cocoa butter and suppository waxes, (9) oils, such as peanut oil. cottonseed oil. safflower oil, sesame oil. olive oil, corn oil and soybean oil, (10) glycols. such as propylene glycol, (11) polyols.
- sugars such as lactose, glucose and sucrose
- starches such as corn starch and potato starch
- cellulose, and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate
- (4) powdered tragacanth such as
- esters such as ethyl oleate and ethyl laurate.
- agar agar.
- buffering agents such as magnesium hydroxide and aluminum hydroxide, (15) algimc acid.
- pyrogen-free water 17.
- lsotomc saline 17.
- Ringer ' s solution (19) ethyl alcohol (20) phosphate buffer solutions, and (21) other non-toxic compatible substances employed in pharmaceutical formulations (see. Remington The Science and Practice of Pharmacy, Nineteenth Edition, Chapter 80 (1995) )
- Formulations of the present invention suitable for oral administration mav be in the form of capsules, cachets, pills, tablets, lozenges (usmg a flavored basis, usually sucrose and acacia or tragacanth), powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in-water or water-in-oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as a gelatm and glycerm, or sucrose and acacia), or as soft elastic gelatm capsules, and/or as mouth washes and the like, each containing a predetermined amount of a compound of the present invention as an active ingredient (5)-procychd ⁇ ne may also be administered as a bolus, electuary or paste
- solid dosage foims of the present invention for oral administration capsules, tablets, pills, dragees, powders, gran
- compositions may also comprise buffering agents
- Solid compositions of a similar type may also be employed as fillers m soft and hard-filled gelatm capsules using such excipients as lactose or milk sugars, as well as high molecular weight polyethylene glycols and the like
- antioxidants examples include (1) water soluble antioxidants, such as ascorbic acid, cysteine hydrochlonde. sodium bisulfate. sodium metabisulfate sodium sulfite and the like, (2) oil-soluble antioxidants. such as ascort l palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecitlun. propyl gallate, alpha-tocopherol, and the like, and (3) metal chelating agents, such as citnc acid, ethylenediamme tetraacetic acid (EDTA). sorbitol. tartanc acid, phosphoric acid, and the luce
- water soluble antioxidants such as ascorbic acid, cysteine hydrochlonde. sodium bisulfate. sodium metabisulfate sodium sulfite and the like
- oil-soluble antioxidants such as ascort l palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene
- a tablet may be made by compression or molding, optionally with one or more accessory mgredients
- Compressed tablets may be prepared usmg binder (for example, gelatm or hydroxypropylmethyl cellulose), lubncant, inert diluent, preservative, dismtegrant (for example, sodium starch glycolate or cross-linked sodium carboxymeth ⁇ 1 cellulose), and/or surface-active or dispersing agents
- Molded tablets may be made by molding in a suitable machine a mixture of the powdered (Sj-procychdine moistened with an inert, liquid diluent
- compositions of the present invention may also be formulated m a soft elastic gelatin capsule unit dosage form by using conventional methods. well-.known m the art (see, e g , Ebert, Pharm Tech , 1(5) 44-50(1977))
- Soft elastic gelatin capsules have a soft, globular, gelatin shell somewhat thicker th-an that of hard gelatin capsules, wherein a gelatm is plasticized by the addition of glycenn, sorbitol, or a similar polyol
- the hardness of the capsule shell may be changed by varying the type of gelatm and the amounts of plasticizer and water
- the soft gelatin shells may contain a preservative to prevent the growth of fungi, such as methyl- and propylparabens and sorbic acid
- the active ingredient may be dissolved or suspended in a liquid vehicle or earner, such as vegetable or mineral oils, glycols such as polyethylene glycol and propylene glycol. t ⁇ glycendes, sur
- the tablets, and other dosage forms of the pharmaceutical compositions of the present mvention may optionally be scored or prepared with coatings and shells, such as entenc coatings and other coatings well .known in the pharmaceutical formulating art
- compositions of the present invention may also be formulated so as to provide slow or controlled release of the active ingredient therein usmg. for example. cellulose in varying proportions to provide the desired release profile, other polymer matrices, hposomes and/or microspheres They may also be administered by controlled release means and delivery devices such as those described in U S Patent Nos 3,845,770, 3.916,899. 3.536.809, 3.598,123. and 4.008.796. and PCT published application WO 92/20377
- the phaimaceutical compositions of the present invention may also optionally contain opacifying agents and may be formulated such that they release the active mgred ⁇ ent(s) only, or preferentially, in a certain portion of the gastrointestinal tract, optionally in a delayed manner
- opacifying agents include polymeric substances and waxes
- the active ingredient can also be m micro- encapsulated form, if appropnate. with one or more of the above-described excipients
- Liquid dosage forms for oral administration of (5)-procychdme include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs
- the liquid dosage forms may contain inert diluents commonly used in the art, such as, for example, water or other solvents, solubilizmg agents and emulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (esp , cottonseed, groundnut, corn, germ, olive, castor and sesame oils), glycerol.
- inert diluents commonly used in the art, such as, for example, water or other solvents, solubilizmg agents and emulsifiers, such as ethyl alcohol, isopropyl alcohol, eth
- the oral compositions of the present invention can also mclude adjuvants such as wetting agents, emulsifying and suspending agents, sweetening. flavoring, coloring, perfuming and preservative agents
- Suspensions in addition to the active (5j-procychdme, may contain suspendmg agents such as, for example, ethoxylated isosteaiyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystallme cellulose, aluminum metahydroxide, bentomte, agar-agar and tragacanth, and mixtures thereof
- suspendmg agents such as, for example, ethoxylated isosteaiyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystallme cellulose, aluminum metahydroxide, bentomte, agar-agar and tragacanth, and mixtures thereof
- Formulations of the pharmaceutical compositions of the present mvention for rectal and vagmal administration may be presented as a suppository, which may be prepared by mixing one or more compounds of the mvention with one or more suitable non-imtating excipients or earners compnsmg. for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate
- suitable non-imtating excipients or earners compnsmg. for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate
- Such formulations of the present mvention are solid at room temperature, but liquid at body temperature and, therefore, will melt m the rectum or vaginal cavity and release the active (S)-procychd ⁇ ne
- Formulations of the present invention which are suitable for vaginal administration also include pessanes. tampons, creams, gels, pastes, foams or foimulations containing such earners as are known in the art to be appropnate
- Dosage forms for the topical or transdermal administration of (Sj-procychdine include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants
- the active compound may be mixed under sterile conditions with a pharmaceutically acceptable earner, and with any preservatives, buffers, or propellants which may be required
- Formulations of the present invention in the form of ointments, pastes, creams and gels may contain, in addition to (S)-procychd ⁇ ne, excipients.
- cellulose derivatives such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth.
- cellulose derivatives polyethylene glycols, sihcones, bentomtes, silicic acid, talc and/or zinc oxide, or mixtures thereof
- Powders and sprays may contain, m addition to (S)-procychd ⁇ ne. excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances Sprays may additionally contain customary propellants, such as, for example, chlorofiuorohydrocarbons, volatile unsubstituted hydrocarbons, hydrocarbon ethers and compressed gases
- Transdermal patches have the added advantage of providmg controlled delivery of the active (S)-procycl ⁇ d ⁇ ne of the present mvention to the body
- dosage foims may be made by dissolving or dispersing the (5)-procychd ⁇ ne m the proper medium Absorption enhancers may also be used to mcrease the flux of the (S)-procychdme across the skin
- the rate of such flux can be controlled by either providing a rate controlling membrane or dispersing the (S)-procychd ⁇ ne in a polymer matrix or gel
- the pharmaceutical compositions of the present mvention are formulated mto pharmaceutically acceptable dosage forms by conventional methods .known to those of skill m the art Where necessary, the pharmaceutical compositions of the present invention are sterile or can be sterilized before admimstration to a patient In a prefened embodiment, the enantiomencally enriched procychdine compositions of the present invention are provided in tablet or capsule
- the capsules or tablets are preferably foimulated with from about 0 25 mg to about 250 mg of (.Sj-procychdine, more preferably with from about 0 50 mg to about 100 mg of (S)-procychd ⁇ ne, and even more preferably with from about 1 mg to about 50 mg of (Sj-procychdine
- the enantiomencally enriched preparations of the present invention are provided m soft elastic gelatin capsule fonn
- the soft elastic gelatin capsules are preferably formulated with from about 0 25 mg to about 250 mg of (5)-procychdme, more preferably with from about 0 50 mg to about 100 mg of (S)-procycl ⁇ d ⁇ ne. and even more preferably with from about 1 mg to about 50 mg of (5)-procycl ⁇ dme
- Actual dosage levels of (S)-procychdme in the pharmaceutical compositions of the present invention may be vaned so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition and mode of administration, without being toxic to the patient
- the selected dosage level and frequency of administration will depend upon a vanety of factors including the route of admimstration, the time of admimstration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used m combination with the (5)-procychd ⁇ ne, the age. sex, weight, condition, general health and prior medical history of the patient being treated. and like factors well known in the medical arts For example, the dosage regimen is likely to vary with pregnant women, nursing mothers and children relative to healthy adults
- a physician having ordinary skill in the art can readily determine and prescribe the effective amount of the pha ⁇ naceutical composition required
- the physician could start doses of the compound employed in the pharmaceutical composition of the present invention at levels lower than that required m order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved
- a suitable daily dose of will be that amount of the compound which is the lowest dose effective to produce a therapeutic effect Such an effective dose will generally depend upon the factors descnbed above Generally, the total daily dose of (5)-procychd ⁇ ne for the conditions descnbed herem may be from about 0 25 mg to about 500 mg, more preferably from about 0 50 mg to about 250 mg, and more preferably from about 1 mg to about 100 mg If desired, the effective daily dose of the active (Sj-procyclidine may be administered as two, three, four, five, six or more sub-doses administered separately at appropriate intervals throughout the day, optionally, in unit dosage forms.
- kits for treating urinary incontinence including, e.g., bladder detrusor muscle instability incontinence, stress incontinence, urge incontinence, overflow incontinence, enuresis, and post-prostectomy incontinence
- said kit comprises a pharmaceutical composition comprising enantiomerically enriched (S)-procyclidine, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier, and instructions for administering enantiomerically enriched (S)-procyclidine for the treatment of urinary incontinence while reducing or eliminating anticholmergic adverse effects associated with racemic procyclidine or other incontinence drugs with anticholmergic action.
- (S)-procyclidine may be established by the following studies of antimuscarinic, spasmolytic, and calcium entry blocking effects in models of receptor binding and bladder function.
- the assays are rapidly filtered under vacuum through GF/B glass fiber filters (available, e.g., from Whatman) and washed with an ice-cold buffer using a Brandel Cell Harvester. Bound radioactivity is determined with a liquid scintillation counter (e.g., LS 6000, Beckman) using a liquid scintillation cocktail (e.g.. Formula 99. DuPont NEN).
- the compounds are tested on each receptor at 10 concentrations in duplicate to obtain competition cui-ves.
- the reference compound for the receptor under investigation is simultaneously tested at 8 concentrations in duplicate to obtain a competition curve in order to validate this experiment.
- the specific radioligand binding of each receptor is defined as the difference between total binding and nonspecific binding determined in the presence of an excess of unlabelled ligand.
- IC 50 values concentration required to inhibit 50% of specific binding
- IC 50 values are determined by non linear regression analysis of the competition curves. These parameters are obtained by curve fitting using SigmaplotTM software. Binding to Calcium Channels
- Binding assays are performed using the methods set forth in Table 2.
- the assays are rapidly filtered under vacuum through GF/B or GF/C glass fiber filters (available, e.g., from Whatman) and washed with an ice-cold buffer using a Brandel Cell Harvester. Bound radio-activity is determined with a liquid scintillation counter (e.g., LS 6000, Beckman) using a liquid scintillation cocktail (e.g., Formula 989, DuPont NEN).
- a liquid scintillation counter e.g., LS 6000, Beckman
- a liquid scintillation cocktail e.g., Formula 989, DuPont NEN
- the compounds are tested in duplicate on each receptor at a concentration of 10 3 M
- the reference compound for the receptor under investigation is simultaneously tested at 8 concentrations in duplicate to obtain a competition cun e m order to validate this experiment
- the specific radiohgand bindmg of each receptor is defined as the difference between total binding and nonspecific binding determined in the presence of an excess of unlabelled ligand Mean values are expressed as a percentage of inhibition of specific binding IC 50 values (concentration required to inhibit 50% of specific binding) are determined by non lmear regression analysis of their competition curves These parameters are obtained by curve fitting usmg SigmaplotTM software
- the tissues are mamtamed at 37 5° C Isometric contractions of the tissues are recorded by using appropnate transducers and an ink- wntmg polygraph A restmg tension of 0 5 grams is mamtamed on each tissue at all times
- the peak tension developed by each strip during the second set of determinations is expressed as a percent of the peak tension developed dunng the first concentration-effect determination.
- the resultant data are analyzed for treatment-related differences by one-way analysis of vanance (ANONA) Since only one concentration of test substance is studied m each strip of bladder, the procedures of Arunlakshana and Schild (1959) are used in modified form to estimate the pA2 and slope of the Schild regression
- the concentrations of agonist producmg a half-maximal response (the EC 50 ) is estimated for each strip from the second set of concentration-effect data
- the EC 50 is obtained from linear regression lmes fit to the loganthm of the concentration of drug and the responses bracketing the half maximum level of response
- a "concentration ratio" (CR) is calculated as the ratio of the EC 50 of the treated tissue divided by the EC 50 ofthe untreated tissue For each exp
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU20182/99A AU2018299A (en) | 1997-12-31 | 1998-12-29 | (s)-procyclidine for treating urinary incontinence |
CA002315843A CA2315843A1 (en) | 1997-12-31 | 1998-12-29 | S-procyclidine for treating urinary incontinence |
JP2000526211A JP2001527039A (en) | 1997-12-31 | 1998-12-29 | S-Procyclidin for treating urinary incontinence |
EP98964973A EP1041981A2 (en) | 1997-12-31 | 1998-12-29 | $i(S)-PROCYCLIDINE FOR TREATING URINARY INCONTINENCE |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7017097P | 1997-12-31 | 1997-12-31 | |
US60/070,170 | 1997-12-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1999033454A2 true WO1999033454A2 (en) | 1999-07-08 |
WO1999033454A3 WO1999033454A3 (en) | 1999-10-21 |
Family
ID=22093580
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/027708 WO1999033454A2 (en) | 1997-12-31 | 1998-12-29 | S-procyclidine for treating urinary incontinence |
Country Status (6)
Country | Link |
---|---|
US (1) | US6130242A (en) |
EP (1) | EP1041981A2 (en) |
JP (1) | JP2001527039A (en) |
AU (1) | AU2018299A (en) |
CA (1) | CA2315843A1 (en) |
WO (1) | WO1999033454A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6376486B1 (en) | 2000-07-06 | 2002-04-23 | American Home Products Corporation | Methods of inhibiting sphincter incontinence |
US6455568B2 (en) | 2000-07-06 | 2002-09-24 | Wyeth | Combination therapy for inhibiting sphincter incontinence |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996031208A2 (en) * | 1995-04-05 | 1996-10-10 | Byk Gulden Lomberg Chemische Fabrik Gmbh | Use of substituted piperidine or pyrrolidine compounds for treating sigma-receptor modulated disorders |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5736577A (en) * | 1995-01-31 | 1998-04-07 | Sepracor, Inc. | Methods and compositions for treating urinary incontinence using optically pure (S)-oxybutynin |
US5034400A (en) * | 1989-10-20 | 1991-07-23 | Olney John W | Method for preventing neurotoxic side effects of NMDA antagonists |
US5677346A (en) * | 1995-01-31 | 1997-10-14 | Sepracor, Inc. | Treating urinary incontinence using (S)-desethyloxybutynin |
US5532278A (en) * | 1995-01-31 | 1996-07-02 | Sepracor, Inc. | Methods and compositions for treating urinary incontinence using optically pure (S)-oxybutynin |
US5939426A (en) * | 1997-02-28 | 1999-08-17 | Sepracor Inc. | Methods for treating urinary incontinence using descarboethoxyloratadine |
-
1998
- 1998-12-29 CA CA002315843A patent/CA2315843A1/en not_active Abandoned
- 1998-12-29 EP EP98964973A patent/EP1041981A2/en not_active Withdrawn
- 1998-12-29 US US09/222,132 patent/US6130242A/en not_active Expired - Fee Related
- 1998-12-29 AU AU20182/99A patent/AU2018299A/en not_active Abandoned
- 1998-12-29 WO PCT/US1998/027708 patent/WO1999033454A2/en not_active Application Discontinuation
- 1998-12-29 JP JP2000526211A patent/JP2001527039A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996031208A2 (en) * | 1995-04-05 | 1996-10-10 | Byk Gulden Lomberg Chemische Fabrik Gmbh | Use of substituted piperidine or pyrrolidine compounds for treating sigma-receptor modulated disorders |
Non-Patent Citations (10)
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6376486B1 (en) | 2000-07-06 | 2002-04-23 | American Home Products Corporation | Methods of inhibiting sphincter incontinence |
US6455568B2 (en) | 2000-07-06 | 2002-09-24 | Wyeth | Combination therapy for inhibiting sphincter incontinence |
US6635660B2 (en) | 2000-07-06 | 2003-10-21 | Wyeth | Methods of inhibiting sphincter incontinence |
Also Published As
Publication number | Publication date |
---|---|
CA2315843A1 (en) | 1999-07-08 |
EP1041981A2 (en) | 2000-10-11 |
WO1999033454A3 (en) | 1999-10-21 |
JP2001527039A (en) | 2001-12-25 |
US6130242A (en) | 2000-10-10 |
AU2018299A (en) | 1999-07-19 |
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