WO1999022754A1 - Method for administering acylated insulin - Google Patents
Method for administering acylated insulin Download PDFInfo
- Publication number
- WO1999022754A1 WO1999022754A1 PCT/US1998/023040 US9823040W WO9922754A1 WO 1999022754 A1 WO1999022754 A1 WO 1999022754A1 US 9823040 W US9823040 W US 9823040W WO 9922754 A1 WO9922754 A1 WO 9922754A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- acylated
- fatty acid
- insulin
- human insulin
- protein
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/28—Insulins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/007—Pulmonary tract; Aromatherapy
- A61K9/0073—Sprays or powders for inhalation; Aerolised or nebulised preparations generated by other means than thermal energy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M15/00—Inhalators
- A61M15/02—Inhalators with activated or ionised fluids, e.g. electrohydrodynamic [EHD] or electrostatic devices; Ozone-inhalators with radioactive tagged particles
Definitions
- the invention is in the field of human medicine. More particularly, the invention is in the field of the treatment of diabetes and hyperglycemia.
- Diabetes mellitus is a serious and chronic disorder that affects 6% of the world's population and all ethnic groups. In the United States, approximately 5% of the population has diabetes. Symptoms of diabetes include hyperglycemia and reduced production or release of insulin. Diabetes mellitus is classified into two types, type I diabetes or insulin-dependent diabetes mellitus (IDDM) and type II diabetes or non-insulin- dependent diabetes mellitus (NIDDM) . Type I diabetes, in which the pancreas has stopped producing insulin, affects
- Type II diabetes is also known as adult onset diabetes.
- the 9-year Diabetes Control and Complications Trial (DCCT) , which involved 1441 type I diabetic patients, demonstrated that maintaining blood glucose levels within close tolerances reduces the frequency and severity of diabetes complications.
- Conventional insulin therapy involves only two injections per day.
- the intensive insulin therapy in the DCCT study involved three or more injections of insulin each day.
- the incidence of diabetes side effects was dramatically reduced. For example, retinopathy was reduced by 50-76%, nephropathy by 35-56%, and neuropathy by 60% in patients employing intensive therapy.
- insulin seems to be an ideal candidate for administration through inhalation into the lungs.
- administration of insulin as an inhalation aerosol to the lung was first reported in 1925.
- numerous human and animal studies have shown that some insulin formulations are well absorbed by the lungs . After administration by inhalation, small-sized proteins are absorbed and reach maximum plasma concentrations more quickly than larger proteins. As expected for a small protein, the previously-studied insulin formulations typically exhibit a rapid rise followed by a rapid fall in plasma insulin levels.
- the present invention is a method for administering long-acting, soluble insulin by inhalation.
- the invention also encompasses the use of a fatty acid-acylated human insulin or a fatty acid-acylated insulin analog in the manufacture of a medicament for the treatment of diabetes or hyperglycemia by inhalation, which treatment comprises administering to a patient in need thereof an effective amount of the medicament using an inhalation device, such that the medicament is deposited in the lungs of the patient.
- the present invention solves two problems currently not addressed by the art. First, previous pulmonary methods for delivering insulin do not provide adequate time action to control blood glucose between meals and overnight.
- soluble, long-acting insulins and insulin derivatives are delivered by subcutaneous injection, which involves the inconvenience of preparing a sample for injection, and the pain of a needle- stick.
- a patient in need of insulin to control blood glucose levels will achieve advantageous slow uptake and prolonged persistence in the blood of acylated insulin compared to inhalation of non- acylated insulin, and reduced inconvenience and pain compared with subcutaneous delivery.
- the acylated insulin is delivered to the lower airway of the patient.
- the acylated insulin can be delivered in a carrier, as a solution or suspension, or as a dry powder, using any of a variety of devices suitable for administration by inhalation.
- the acylated insulin can be administered using an inhalation device such as a nebulizer, a metered-dose inhaler, a dry powder inhaler, a sprayer, and the like.
- the acylated insulin is delivered in a particle size effective for reaching the lower airways of the lung, preferably less than about 10 microns mass median aerodynamic diameter (MMAD) , preferably about 1 to about 5 microns MMAD, and more preferably about 1 to about 3 microns MMAD or from about 1 to about 2 microns MMAD, and most preferably from about 2 to about 3 microns MMAD.
- Preferred acylated insulins include a fatty acid-acylated insulin and a fatty acid-acylated insulin analog.
- the invention also provides a method for administering acylated insulin or acylated insulin analog together with insulin or insulin analog to a patient in need thereof by inhalation.
- Administering such combinations of acylated and un-acylated insulins provides both post-prandial and basal control of blood glucose levels. Because the method avoids injections, patient comfort is improved, and patient compliance increased compared with conventional insulin delivery methods .
- fatty acid-acylated insulin protein refers to a protein, selected from the group consisting of mammalian insulin and mammalian insulin analogs, that is acylated with a fatty acid that is bonded through an amide bond formed between the acid group of the fatty acid and an amino group of the protein, and pharmaceutically acceptable salts and complexes thereof .
- the amino group may be the ⁇ - amino group of an N-terminal amino acid of the protein, or may be the ⁇ -amino group of a Lys residue of the protein.
- the acylation of insulin with a fatty acid is disclosed in Japanese patent application 1-254,699. See also, Hashimoto, et al . , Pharmaceutical Research, 6:171-176 (1989), and
- a fatty acid-acylated insulin or insulin analog may be acylated at one or more of the three amino groups that are present in mammalian insulin and in most insulin analogs.
- Mono-acylated insulins are acylated at a single amino group.
- Di-acylated insulins are acylated at two amino groups.
- Tri- acylated insulins are acylated at three amino groups.
- the terms "fatty acid-acylated insulin” and "fatty acid-acylated insulin analog” refer to preparations of fatty acid-acylated insulin and fatty acid-acylated insulin analogs, respectively, which are herein referred to collectively as fatty acid-acylated insulin protein.
- the population of acylated molecules is relatively homogeneous with respect to the site or sites of acylation, such as: N ⁇ - mono-acylated insulin protein, Bl-N ⁇ -mono-acylated insulin or insulin analog; Al-N ⁇ -mono-acylated insulin or insulin analog; Al-N ⁇ , Bl-N ⁇ -di-acylated insulin or insulin analog; N ⁇ , Al-N ⁇ -di-acylated insulin or insulin analog; N ⁇ , Bl-N ⁇ - di-acylated insulin or insulin analog, and N ⁇ , Al-N ⁇ , Bl-N ⁇ - tri-acylated insulin or insulin analog.
- acylation such as: N ⁇ - mono-acylated insulin protein, Bl-N ⁇ -mono-acylated insulin or insulin analog; Al-N ⁇ -mono-acylated insulin or insulin analog; Al-N ⁇ , Bl-N ⁇ -di-acylated insulin or insulin analog; N ⁇ , Al-N ⁇ -di-acylated insulin or insulin analog; N ⁇ ,
- fatty acid-acylated insulin and fatty acid-acylated insulin analog also refer to preparations wherein the population of acylated molecules has heterogeneous acylation. In the latter case, these terms include mixtures of mono-acylated and di-acylated proteins, mixtures of mono-acylated and tri- acylated proteins, mixtures of di-acylated and tri-acylated proteins, and mixtures of mono-acylated, di-acylated, and tri-acylated proteins.
- Preferred acylated insulins include mono-acylated insulin, particularly mono-acylated insulin acylated at positions Bl or B29, preferably B29.
- Preferred acylated insulin analogs are mono-acylated at Bl, or at the N ⁇ -amino group of lysine.
- preferred fatty acid- acylated insulin proteins are B29-N ⁇ -palmitoyl-human insulin, B29-N ⁇ -myristoyl-desB30-human insulin, B29-N ⁇ - myristoyl-human insulin, B28-N ⁇ -palmitoyl-LysB28, ProB29- human insulin analog, and B28-N ⁇ -myristoyl- ysB28, ProB29- human insulin analog.
- acylate means to form an amide bond between an organic acid and an amino group of a protein.
- An insulin is “acylated” when one or more of its amino groups is combined in an amide bond with the acid group of an organic acid.
- insulin or insulin analogs are acylated by reacting them with an activated acid compound.
- Activated acids are acyl compounds having carboxylic acid activating groups, such as, activated ester, acid halide, acid azolide, or acid anhydride, and in particular, hydroxybenzotriazide (HOBT) , N-hydroxysuccinimide, and derivatives thereof .
- HOBT hydroxybenzotriazide
- N-hydroxysuccinimide and derivatives thereof .
- N-hydroxysuccinimide esters of fatty acids is a particularly advantageous means of acylating a free amino acid with a fatty acid.
- activated fatty acid ester means a fatty acid which has been activated using general techniques known in the art [Methods of Enzymology, 25:494-499 (1972) and Lapidot, et al . J. of Lipid Res . 8:142-145 (1967)].
- Lapidot, et al describe the preparation of N-hydroxysuccinimide esters and their use in the preparation of N-lauroyl-glycine, N-lauroyl-L-serine, and N- lauroyl-L-glutamic acid.
- various protecting groups may be used to block the ⁇ -amino group during the coupling.
- the selection of a suitable protecting group is known to one skilled in the art and includes, for example, p-methoxybenzoxycarbonyl (pmZ) .
- the ⁇ - amino group is acylated in a one-step synthesis without the use of amino-protecting groups.
- a process for selective acylation at the N ⁇ -amino group of Lys in insulin is disclosed and claimed in U.S. Patent No. 5,646,242, issued 8 July 1997, the entire disclosure of which is incorporated expressly by reference.
- a process for preparing a dry powder of an acylated protein is disclosed in U.S. Patent No. 5,700,904, issued 23 December, 1997, the entire disclosure of which is incorporated expressly herein by reference .
- fatty acid means a saturated or unsaturated fatty acid having from 6 to 18 carbon atoms. Preferred fatty acids have from 10 to 16 carbon atoms. Yet a more preferred group of fatty acids have from 13 to 17 carbon atoms . A highly preferred group of fatty acids have 14 or 16 carbon atoms, and more preferably 14 carbon atoms.
- insulin refers to mammalian insulin, such as bovine, porcine or human insulin, whose sequences and structures are known in the art. Bovine, porcine, and human insulin are preferred mammalian insulins; human insulin is more preferred. The amino acid sequence and spatial structure of human insulin are well-known.
- Human insulin is comprised of a twenty-one amino acid A- chain and a thirty amino acid B-chain which are cross-linked by disulfide bonds.
- a properly cross-linked human insulin contains three disulfide bridges: one between position 7 of the A-chain and position 7 of the B-chain, a second between position 20 of the A-chain and position 19 of the B-chain, and a third between positions 6 and 11 of the A-chain.
- insulin analog means proteins that have an A-chain and a B-chain that have substantially the same amino acid sequences as the A-chain and B-chain of human insulin, respectively, but differ from the A-chain and B-chain of human insulin by having one or more amino acid deletions, one or more amino acid replacements, and/or one or more amino acid additions that do not destroy the insulin activity of the insulin analog.
- Preferred insulin analogs include the monomeric insulin analogs, and desB30 human insulin analog.
- One type of insulin analog is well-known in the art. These are fast-acting analogs of human insulin, including, for example, human insulin wherein Pro at position B28 is substituted with Asp, Lys, Leu, Val, or Ala, and wherein Lys at position B29 is Lys or is substituted with Pro, and also, AlaB26-human insulin, des(B28-B30) human insulin, and des(B27) human insulin.
- monomeric insulin analogs are disclosed in Chance, et al . , U.S. Patent No. 5,514,646, issued May 7, 1996; Chance, et al . , U.S. Patent Application Serial No. 08/255,297; Brems, et al .
- Gin may be replaced with Asp or Glu.
- AsnAl ⁇ , AsnA21, or AsnB3, or any combination of those residues may be replaced by Asp or Glu.
- GlnA15 or GlnB4 , or both may be replaced by either Asp or Glu.
- Particularly preferred insulin analogs are those having, optionally, among other replacements or deletions, Asp at B21, or Asp at B3, or both replacements .
- Insulin and insulin analogs used to prepare the fatty acid-acylated insulins that are administered in the present invention can be prepared by any of a variety of recognized peptide synthesis techniques including classical (solution) methods, solid phase methods, semi-synthetic methods, and more recent recombinant DNA methods.
- complex means a compound in which a transition metal is coordinated to at least one ligand.
- Ligands include nitrogen-containing molecules, such as insulins, peptides, amino acids, and TRIS, among many other compounds.
- the fatty acid-acylated insulin or fatty acid- acylated insulin analog used in the present invention are preferably in a complex with one or more divalent zinc ions, wherein the protein molecule acts a ligand of the zinc ions.
- preservative refers to a compound added to a pharmaceutical formulation to act as an anti-microbial agent. A parenteral formulation must meet guidelines for preservative effectiveness to be a commercially viable multi-use product.
- preservatives known in the art as being effective and acceptable in parenteral formulations are benzalkonium chloride, benzethonium, chlorohexidine, phenol, m-cresol, benzyl alcohol, methylparaben, chlorobutanol, o-cresol, p-cresol, chlorocresol, phenylmercuric nitrate, thimerosal, benzoic acid, and various mixtures thereof. See, e . g. , allhauser, K.,
- phenol and m-cresol are known to bind to insulin-like molecules and thereby to induce conformational changes that increase either physical or chemical stability, or both [Birnbaum, et al . , Pharmac. Res . 14:25 (1997); Rahuel-Clermont , et al . , Biochemistry 36:5837-5845 (1997)].
- M-cresol and phenol are preferred preservatives in formulations of the fatty acid-acylated insulin proteins used in the present invention.
- buffer or “pharmaceutically acceptable buffer” refers to a compound that is known to be safe for use in insulin formulations and that has the effect of controlling the pH of the formulation at the pH desired for the formulation.
- Pharmaceutically acceptable buffers for controlling pH at a moderately acid pH to a moderately basic pH include such compounds as phosphate, acetate, citrate, TRIS, arginine, or histidine.
- isotonicity agent refers to a compound that is tolerated physiologically and imparts a suitable tonicity to a formulation to prevent the net flow of water across the cell membrane.
- Compounds, such as glycerin, are commonly used for such purposes at known concentrations.
- Other acceptable isotonicity agents include salts, e . g. , NaCl, dextrose, mannitol, and lactose. Glycerol at a concentration of 12 to 25 mg/mL is preferred as an isotonicity agent.
- MMAD and "MMEAD” are well-known in the art, and stand for “mass median aerodynamic diameter” and “mass median equivalent aerodynamic diameter,” respectively. The terms are substantially equivalent.
- the "aerodynamic equivalent” size of a particle is the diameter of a unit density sphere which exhibits the same aerodynamic behavior as the particle, regardless of actual density or shape.
- MMAD is determined using a cascade impactor, which measures the particle size as a function of the aerodynamic behavior of the particle in a high velocity airstream.
- the median (50%) particle size is obtained from a linear regression analysis of the cumulative distribution data.
- One vehicle for a fatty acid-acylated insulin protein is Humulin ® R Diluent.
- a formulation with this vehicle includes a fatty acid-acylated insulin protein at the desired concentration, m-cresol at about 2.5 mg/ml, glycerol at about 16 mg/mL, and zinc at about 0.016 mg/mL, the formulation being at pH about 7.4.
- Humulin ® L Diluent which includes a fatty acid-acylated insulin protein at the desired concentration; zinc oxide at about 0.12 mg/mL to about 0.3 mg/mL, preferably about 0.17 mg/mL; sodium acetate at about 6.5 mg/mL to about 7.5 mg/mL, preferably about 7.0 mg/mL; and methylparaben at about 0.8 mg/mL to about 1.2 mg/mL, preferably about 1.0 mg/mL.
- Certain formulations of insulin protein with zinc are known as Lente ® insulins. Additional suitable formulations of insulin protein with zinc and of Lente ® insulins are known to those of skill in the art.
- Fatty acid-acylated insulin activity is administered by inhalation in a dose effective to increase circulating insulin protein levels and/or to lower circulating glucose levels. Such administration can be effective for treating disorders such as diabetes or hyperglycemia.
- Achieving effective doses of fatty acid-acylated insulin protein requires administration of an inhaled dose of more than about 0.5 ⁇ g/kg to about 200 ⁇ g/kg fatty acid-acylated insulin protein.
- the dose is about 5 ⁇ g/kg to about 100 ⁇ g/kg, about 10 ⁇ g/kg to about 100 ⁇ g/kg, about 20 ⁇ g/kg to about 100 ⁇ g/kg, or about 30 ⁇ g/kg to about 100 ⁇ g/kg.
- the dose is from about 10 ⁇ g/kg to about 60 ⁇ g/kg, 20 ⁇ g/kg to about 60 ⁇ g/kg, or 30 ⁇ g/kg to about 60 ⁇ g/kg.
- a therapeutically effective amount can be determined by a knowledgeable practitioner, who will take into account factors including insulin protein level, the physical condition of the patient, the patient's pulmonary status, the potency and bioavailability of the fatty acid- acylated insulin protein, whether the fatty acid-acylated protein is administered together with another insulin, such as a fast-acting, or meal-time insulin, or with other therapeutic agents, or other factors known to the medical practitioner.
- Effective therapy can include starting at a low dose of fatty acid-acylated insulin protein, monitoring blood glucose levels, and increasing the dose of fatty acid- acylated insulin protein as required to achieve desired blood glucose levels.
- fatty acid-acylated insulin protein is delivered by inhalation to achieve advantageous slow uptake of fatty acid-acylated insulin protein compared to inhalation of non-acylated insulin protein.
- Administration by inhalation results in pharmacokinetics comparable to subcutaneous administration of insulins.
- Subcutaneous injection of insulins, such as fatty acid- acylated insulin protein typically results in a slow rise in the level of insulin until blood levels reach a maximum several hours, typically about 3 hours, after injection.
- Levels of insulins such as fatty acid-acylated insulin proteins, then, typically, drop to basal levels with a half- life of about 5 to about 8 hours.
- Inhalation of native or other non-acylated forms of insulin leads to a rapid rise in the level of circulating insulin followed by a rapid fall in insulin level.
- levels of non-acylated insulin rise to a maximum in only about 20 to about 30 min and return to basal levels with a half-life of about one hour.
- inhalation of fatty acid- acylated insulin protein results in slow uptake of the fatty acid-acylated insulin protein into the blood followed by a slow fall in the blood level .
- inhaled fatty acid-acylated insulin protein reaches peak levels in the blood about 3 to about 5 hours after inhalation, followed by a return to basal levels with a half-life of about 5 to about 8 hours, preferably about 5 hours.
- Different inhalation devices typically provide similar pharmacokinetics when similar particle sizes and similar levels of lung deposition are compared.
- a fatty acid- acylated insulin protein can be delivered by any of a variety of inhalation devices and methods known in the art for administration of insulin, or other proteins, by inhalation [Rubsamen, U.S. Patent No. 5,364,838, issued 15 November, 1994; Rubsamen, U.S. Patent No.
- acylated insulins include those well- known in the art, such as, metered dose inhalers, liquid nebulizers, dry powder inhalers, sprayers, thermal vaporizers, and the like, and those provided by developing technology, including the AERx ® pulmonary drug delivery system being developed by Aradigm Corporation, the dry powder formulation and delivery devices being developed by Inhale Therapeutic Systems, Inc., and the Spiros ® dry powder inhaler system being developed by Dura Pharmaceuticals, Inc. Other suitable technology includes electrohydrodynamic aerosolizers.
- the inhalation device should deliver small particles, e . g.
- MMAD less than about 10 ⁇ m MMAD, preferably about 1-5 ⁇ m MMAD, for good respirability, and more preferably in the range of about 1 to about 3 ⁇ m MMAD, or about 1 to about 2 ⁇ m MMAD, and most preferably from about 2 to about 3 ⁇ m MMAD.
- the inhalation device must be practical, in the sense of being easy to use, small enough to carry conveniently, capable of providing multiple doses, and durable.
- Some specific examples of commercially available inhalation devices suitable for the practice of this invention are Turbohaler (Astra) , Rotahaler (Glaxo) , Diskus (Glaxo) , the Ultravent nebulizer (Mallinckrodt) , the Acorn II nebulizer (Marquest Medical Products) , the Ventolin metered dose inhaler (Glaxo) , the Spinhaler powder inhaler (Fisons) , or the like.
- Fatty acid-acylated insulin proteins can be advantageously delivered by a dry powder inhaler or a sprayer.
- a dry powder inhalation device for administering fatty acid- acylated insulin protein.
- delivery by such inhalation devices is advantageously reliable, reproducible, and accurate.
- the formulation of fatty acid-acylated insulin protein, the quantity of the formulation delivered, and the duration of administration of a single dose depend on the type of inhalation device employed.
- the frequency of administration and length of time for which the system is activated will depend mainly on the concentration of fatty acid-acylated insulin protein in the aerosol. For example, shorter periods of administration can be used at higher concentrations of fatty acid-acylated insulin protein in the nebulizer solution.
- Devices such as metered dose inhalers can produce higher aerosol concentrations, and can be operated for shorter periods to deliver the desired amount of fatty acid-acylated insulin protein.
- Devices such as dry powder inhalers deliver active agent until a given charge of agent is expelled from the device. In this type of inhaler, the amount of fatty acid-acylated insulin protein in a given quantity of the powder determines the dose delivered in a single administration.
- the particle size of the fatty acid-acylated insulin protein formulation delivered by the inhalation device determines the extent to which the particles are conveyed into the lower airways or alveoli, where deposition is most advantageous because of the large surface area.
- the formulation of the fatty acid-acylated insulin protein will affect the particle size.
- the fatty acid- acylated insulin protein is formulated so that at least about 10% of the fatty acid-acylated insulin protein is deposited in the lower lung, preferably about 10% to about 20%, or more. It is known that the maximum efficiency of pulmonary deposition for mouth-breathing humans is obtained at about 2 ⁇ m to about 3 ⁇ m MMAD. Above about 5 ⁇ m MMAD, pulmonary deposition decreases substantially.
- particles of fatty acid-acylated insulin delivered by inhalation have a particle size less than about 10 ⁇ m, preferably in the range of about 1 ⁇ m to about 5 ⁇ m MMAD, and more preferably in the range of about 1 to about 3 ⁇ m MMAD or from about 1 to about 2 ⁇ m MMAD, and most preferably from about 2 to about 3 ⁇ m MMAD.
- the formulation of fatty acid-acylated insulin is selected to yield the desired particle size in the chosen inhalation device .
- Dry powder generation typically employs a method such as a scraper blade or an air blast to generate particles from a solid formulation of fatty acid-acylated insulin protein.
- the particles are generally generated in a container and then transported into the lung of a patient via a carrier air stream.
- the force for breaking up the solid and air flow is provided solely by the patient's inhalation.
- One suitable dry powder inhaler is the Turbohaler manufactured by Astra.
- Administration by dry powder inhaler is a preferred method for fatty acid-acylated insulin protein.
- Formulations of fatty acid-acylated insulin protein for administration from a dry powder inhaler typically include a finely divided dry powder containing fatty acid-acylated insulin protein, but the powder can also include a non-acylated insulin or insulin analog to provide relatively rapid onset, and short duration of action, a bulking agent, buffer, carrier, excipient, another additive, or the like.
- Additives can be included in a dry powder formulation of fatty acid-acylated insulin protein, for example, to dilute the powder as required for delivery from the particular powder inhaler, to facilitate processing of the formulation, to provide advantageous powder properties to the formulation, to facilitate dispersion of the powder from the inhalation device, to stabilize to the formulation (e.g., antioxidants or buffers), to provide taste to the formulation, or the like.
- the additive does not adversely affect the patient's airways.
- the fatty acid-acylated insulin protein can be mixed with an additive at a molecular level or the solid formulation can include particles of the fatty acid-acylated insulin protein mixed with or coated on particles of the additive.
- Typical additives include mono-, di-, and polysaccharides; sugar alcohols and other polyols, such as, for example, lactose, glucose, raffinose, melezitose, lactitol, maltitol, trehalose, sucrose, mannitol, starch, or combinations thereof; surfactants, such as sorbitols, diphosphatidyl choline, or lecithin; or the like.
- an additive such as a bulking agent, is present in an amount effective for a purpose described above, often at about 50% to about 90% by weight of the formulation. Additional agents known in the art for formulation of a protein such as fatty acid-acylated insulin protein can also be included in the formulation.
- fatty acid-acylated insulin protein is prepared in a particulate form with an MMAD of less than about 10 microns, preferably about 1 to about 5 microns, and more preferably in the range of about 1 to about 3 ⁇ m MMAD, or from about 1 to about 2 ⁇ m MMAD, and, most preferably, from about 2 to about 3 ⁇ m MMAD.
- the preferred particle size is effective for delivery to the alveoli of the patient's lung.
- the dry powder is largely composed of particles produced so that a majority of the particles have a size in the desired range.
- At least about 50% of the dry powder is made of particles having a diameter less than about 10 ⁇ m MMAD.
- Such formulations can be achieved by spray drying, milling, or critical point condensation of a solution containing fatty acid-acylated insulin protein and other desired ingredients .
- a spray including fatty acid-acylated insulin protein can be produced by forcing a suspension or solution of fatty acid-acylated insulin protein through a nozzle under pressure.
- the nozzle size and configuration, the applied pressure, and the liquid feed rate can be chosen to achieve the desired output and particle size.
- An electrospray can be produced by an electric field in connection with a capillary or nozzle feed.
- particles of fatty acid-acylated insulin protein delivered by a sprayer have a particle size less than about 10 ⁇ m, preferably in the range of about 1 ⁇ m to about 5 ⁇ m MMAD, and more preferably in the range of about 1 to about 3 ⁇ m MMAD, or about 1 to about 2 ⁇ m MMAD, and most preferably from about 2 to about 3 ⁇ m MMAD.
- Administration as a spray is a preferred method for fatty acid-acylated insulin protein.
- Formulations of fatty acid-acylated insulin protein suitable for use with a sprayer typically include fatty acid-acylated insulin protein in an aqueous solution at a concentration of about 1 mg to about 20 mg of fatty acid- acylated insulin protein per mL of solution.
- the formulation can include agents such as an excipient, a buffer, an isotonicity agent, a preservative, a surfactant, and, preferably, zinc.
- the formulation can also include an excipient or agent for stabilization of the fatty acid- acylated insulin protein, such as a buffer, a reducing agent, a bulk protein, or a carbohydrate.
- Bulk proteins useful in formulating fatty acid-acylated insulin proteins include albumin, protamine, or the like.
- Typical carbohydrates useful in formulating fatty acid-acylated insulin proteins include sucrose, mannitol, lactose, trehalose, glucose, or the like.
- the fatty acid-acylated insulin protein formulation can also include a surfactant, which can reduce or prevent surface-induced aggregation of the fatty acid-acylated insulin protein caused by atomizati ⁇ n of the solution in forming an aerosol.
- a surfactant can be employed, such as polyoxyethylene fatty acid esters and alcohols, and polyoxyethylene sorbitol fatty acid esters. Amounts will generally range between 0.001% and 4% by weight of the formulation.
- Especially preferred surfactants for purposes of this invention are polyoxyethylene sorbitan monooleate, polysorbate 80, polysorbate 20, or the like. Additional agents known in the art for formulation of a protein such as fatty acid-acylated insulin protein can also be included in the formulation.
- Fatty acid-acylated insulin protein can be administered by a nebulizer, such as jet nebulizer or an ultrasonic nebulizer.
- a nebulizer such as jet nebulizer or an ultrasonic nebulizer.
- a compressed air source is used to create a high-velocity air jet through an orifice.
- a low- pressure region is created, which draws a solution of fatty acid-acylated insulin protein through a capillary tube connected to a liquid reservoir.
- the solution streaming from the capillary tube is sheared into unstable filaments and droplets as it exits the tube, creating an aerosol.
- a range of configurations, flow rates, and baffle types can be employed to achieve the desired performance characteristics from a given jet nebulizer.
- particles of fatty acid-acylated insulin protein delivered by a nebulizer have a particle size less than about 10 ⁇ m, preferably in the range of about 1 ⁇ m to about 5 ⁇ m MMAD, and more preferably in the range of about 1 to about 3 ⁇ m MMAD, or about 1 to about 2 ⁇ m MMAD, and most preferably from about 2 to about 3 ⁇ m MMAD.
- Formulations of fatty acid-acylated insulin protein suitable for use with a nebulizer, either jet or ultrasonic typically include fatty acid-acylated insulin protein in an aqueous solution at a concentration of about 1 mg to about 20 mg of fatty acid-acylated insulin protein per mL of solution.
- the formulation can include agents such as an excipient, a buffer, an isotonicity agent, a preservative, a surfactant, and, preferably, zinc.
- the formulation can also include an excipient or agent for stabilization of the fatty acid-acylated insulin protein, such as a buffer, a reducing agent, a bulk protein, or a carbohydrate.
- Bulk proteins useful in formulating fatty acid-acylated insulin proteins include albumin, protamine, or the like.
- Typical carbohydrates useful in formulating fatty acid-acylated insulin proteins include sucrose, mannitol, lactose, trehalose, glucose, or the like.
- the fatty acid-acylated insulin protein formulation can also include a surfactant, which can reduce or prevent surface-induced aggregation of the fatty acid-acylated insulin protein caused by atomization of the solution in forming an aerosol.
- Various conventional surfactants can be employed, such as polyoxyethylene fatty acid esters and alcohols, and polyoxyethylene sorbital fatty acid esters . Amounts will generally range between 0.001 and 4% by weight of the formulation.
- Especially preferred surfactants for purposes of this invention are polyoxyethylene sorbitan monooleate, polysorbate 80, polysorbate 20, or the like. Additional agents known in the art for formulation of a protein such as fatty acid-acylated insulin protein can also be included in the formulation.
- a metered dose inhaler MDI
- a propellant, fatty acid-acylated insulin protein, and any excipients or other additives are contained in a canister as a mixture including a liquefied compressed gas.
- Actuation of the metering valve releases the mixture as an aerosol, preferably with a MMAD in the range of less than about 10 ⁇ m, preferably about 1 ⁇ m to about 5 ⁇ m, and more preferably in the range of about 1 to about 3 ⁇ m MMAD, or about 1 to about 2 ⁇ m MMAD, and, most preferably from about 2 to about 3 ⁇ m MMAD.
- the desired aerosol particle size can be obtained by employing a formulation of fatty acid-acylated insulin protein produced by various methods known to those of skill in the art, including jet-milling, spray drying, critical point condensation, or the like.
- Preferred metered dose inhalers include those manufactured by 3M or Glaxo and employing a hydrofluorocarbon propellant.
- Formulations of fatty acid-acylated insulin protein for use with a metered-dose inhaler device will generally include a finely divided powder containing fatty acid- acylated insulin protein as a suspension in a non-aqueous medium, for example, suspended in a propellant with the aid of a surfactant.
- the propellant may be any conventional material employed for this purpose, such as chlorofluorocarbon, a hydrochlorofluorocarbon, a hydrofluorocarbon, or a hydrocarbon, including trichlorofluoromethane, dichlorodifluoromethane, dichlorotetrafluoroethanol and 1, 1, 1, 2-tetrafluoroethane, HFA-134a (hydrofluroalkane-134a) , HFA-227 (hydrofluroalkane- 227) , or the like.
- the propellant is a hydrofluorocarbon.
- the surfactant can be chosen to stabilize the fatty acid-acylated insulin protein as a suspension in the propellant, to protect the active agent against chemical degradation, and the like.
- Suitable surfactants include sorbitan trioleate, soya lecithin, oleic acid, or the like. In some cases solution aerosols are preferred using solvents such as ethanol .
- Additional agents known in the art for formulation of a protein such as fatty acid-acylated insulin protein can also be included in the formulation.
- the present invention also relates to a pharmaceutical composition including fatty acid-acylated insulin protein and suitable for administration by inhalation. According to the invention, fatty acid-acylated insulin protein can be used for manufacturing a composition or medicament suitable for administration by inhalation.
- the invention also relates to methods for manufacturing compositions including fatty acid-acylated insulin protein in a form that is suitable for administration by inhalation.
- a dry powder formulation can be manufactured in several ways, using conventional techniques, such as described in any of the publications mentioned above and incorporated expressly herein by reference, and, for example, Baker, et al . , U.S. Patent No. 5,700,904, issued 23 December, 1997, the entire disclosure of which is incorporated expressly herein by reference. Particles in the size range appropriate for maximal deposition in the lower respiratory tract can be made by micronizing, milling, or the like.
- a liquid formulation can be manufactured by dissolving the fatty acid-acylated insulin protein in a suitable solvent, such as water, at an appropriate pH, including buffers or other excipients.
- B29-N ⁇ -palmitoyl-human insulin is a soluble human insulin derivative that has a prolonged hypoglycemic profile in normal pigs and pancreatized dogs comparable to Humulin ® L (Lente ® ) insulin, which is a formulation containing insulin is an solid form that dissolves slowly after injection.
- Each animal was dosed using two dosing regimens: a) intravenous injection (7 ⁇ g B29-N ⁇ -palmitoyl-human insulin per kg of body weight) and b) aerosol delivery to the lung via an endotracheal tube (target lung does of 100 ⁇ g B29-N ⁇ - palmitoyl-human insulin per kg of body weight) . Following anesthesia using 2% Brevital ® , each animal was intubated using a size 5 or 6 endotracheal tube .
- Aerosol delivery to the lung was achieved by connecting the endotracheal tube to an ultrasonic nebulizer (Model 25, Devilbiss Co., Somerset, PA) containing ⁇ 4 mg B29-N ⁇ -palmitoyl-human insulin per mL of Humulin ® R Diluent and 99mTc sulfur colloid.
- This system operated in a passive mode delivered the aerosol to the anesthetized animal through a one-way valve connected to the endotracheal tube.
- the target dose using the ultrasonic nebulizer was 100 ⁇ g/kg.
- Each animal was placed under a gamma camera for about a 37 minute exposure period in order to collect a scintiphoto of the lung to estimate total dose delivered.
- Blood samples were collected at 0 (pre) , 5, 10, 20, 40, 60, 120, 180, 240, 360, 480, 960, 1440, 2160, and 2880 minutes post-dose to measure B29-N ⁇ -palmitoyl-human insulin in the blood. Blood samples were only collected until 1440 minutes post-dose after intravenous dosing. Serum concentration of immunoreactive B29-N ⁇ -palmitoyl-human insulin was determined by a radioimmunoassay.
- Serum concentration of immunoreactive B29-N ⁇ -palmitoyl-human insulin in dogs prior to the exposure of the drug is a measure of the endogenous insulin level, because the antiserum used in the radioimmunoassay recognized insulin as well as B29-N ⁇ -palmitoyl-human insulin.
- Particle size distribution was determined using a Sierra cascade impactor (model 218-K ambient cascade impactor, Sierra Instruments, Carmel Valley, CA) .
- deposited lung doses ranged from 10.2 to 60.3 ⁇ g B29-N ⁇ -palmitoyl-human insulin per kg of body weight as determined by gamma camera scintigraphy.
- the mass median aerodynamic diameter (MMAD) was estimated to be 5.67 ⁇ m, with a geometric standard deviation of 2.04 ⁇ m.
- Pharmacokinetic parameters were calculated from validated software programs at Lilly Research Laboratories, Indianapolis, IN. Serum concentrations below the quantitation limit (BQL) of 17 pM were assigned a zero value for the subsequent calculations except when the average of the pre-dose levels was calculated where BQLs were not used.
- Area under the curve (AUC) values were determined in two ways. In the first method, the area under the curve was calculated only from 0 to 16 hours post-dose [AUC (0-16 h) ] .
- the area under the curve was calculated from 0 to t hours post-dose [AUC (0-t h) .
- the time "t" was defined as the last time point at which the serum concentration appeared to be higher than the baseline insulin level (i.e., ⁇ 110 pM) .
- the actual points not used in the AUC calculations are indicated by (**) in Table 1.
- B29-N ⁇ -palmitoyl-human insulin serum concentrations returned to baseline level by 24 or 48 hours post-dose for dogs receiving B29-N ⁇ -palmitoyl-human insulin by intravenous administration or inhalation, respectively.
- Tl/2 (half-life) values for inhalation were comparable to those obtained by intravenous administration, and were on the order of several hours (Table 2) .
- Tl/2 values in the present study were comparable to those measured in a previous study following subcutaneous administration of B29- N ⁇ -palmitoyl-human insulin.
- AUC (0-t) values were determined in two ways.
- the bioavailability of inhaled B29-N ⁇ -palmitoyl-human insulin in individual dogs ranged from 3.4% to 16.3% (Table 2), with an average of about 8%.
- the bioavailability of inhaled B29-N ⁇ - palmitoyl-human insulin in individual dogs ranged from 4.1% to 24.0%, with an average of about 10%.
- the bioavailability of B29-N ⁇ -palmitoyl-human insulin administered by the pulmonary route can be compared with the bioavailability of the same molecule, given subcutaneously to normal dogs in a previous study.
- the average AUC (0-24 hr) was determined to be 122.8 nM*hr, or -2.46 nM*hr/ ⁇ g/kg.
- the relative bioavailability of inhaled B29-N ⁇ - palmitoyl-human insulin is about 23% that obtained by subcutaneous administration.
- the average AUC (0-t) value for inhalation was normalized for the dose administered, because the average AUC value for the subcutaneous dose was calculated based on drug concentrations from 0-24 hours.
- the absorption of the acylated insulin after pulmonary administration is estimated at 11-14% compared with subcutaneous administration.
- the mean inhaled doses were 222 ⁇ g/kg for B28-N ⁇ -myristoyl-LysB28, ProB29-human insulin analog and 36 ⁇ g/kg for B29-N ⁇ -palmitoyl-human insulin.
- Table 3 shows serum immunoreactive levels of B28-N ⁇ - myristoyl-LysB28,ProB29-human insulin analog and blood glucose levels following administration by pulmonary or subcutaneous delivery.
- the relative bioavailability of B28-N ⁇ -myristoyl- LysB28, ProB29-human insulin analog deposited in the lung compared to that injected subcutaneously was 11.2% as determined by comparing the maximum blood levels when normalized for the same dose delivered to the body, i . e . , either deposited in the lung below the trachea or injected subcutaneously into the body.
- the relative bioavailability of inhaled to subcutaneous B29-N ⁇ -palmitoyl-human insulin was 22%. These relative bioavailabilities of 11% and 22% should be compared to the values of approximately 40% to 50' for non-acylated insulin that have been observed in previous animal studies conducted at Eli Lilly [Pillai, R.
- Both B28-N ⁇ -myristoyl-LysB28, ProB29-human insulin analog and B29-N ⁇ -palmitoyl-human insulin are well-absorbed through the dog lung. Although the degree of absorption is less than that of non-acylated insulin, the fatty acid- acylated insulins are absorbed in an amount effective to reduce glucose levels. The pharmacokinetics of the subcutaneous and inhaled insulins are quite similar for fatty acid-acylated insulins.
- One of skill in the art recognizes from these data that other fatty acid-acylated insulins and fatty acid-acylated insulin analogs are also absorbed from the lung, since these data are obtained with two representative molecules.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000518685A JP2001521904A (en) | 1997-10-31 | 1998-10-29 | How to administer acylated insulin |
AU12897/99A AU1289799A (en) | 1997-10-31 | 1998-10-29 | Method for administering acylated insulin |
CA002308136A CA2308136A1 (en) | 1997-10-31 | 1998-10-29 | Method for administering acylated insulin |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6443997P | 1997-10-31 | 1997-10-31 | |
US60/064,439 | 1997-10-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999022754A1 true WO1999022754A1 (en) | 1999-05-14 |
Family
ID=22055992
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/023040 WO1999022754A1 (en) | 1997-10-31 | 1998-10-29 | Method for administering acylated insulin |
Country Status (7)
Country | Link |
---|---|
US (2) | US6051551A (en) |
EP (1) | EP0919242A3 (en) |
JP (1) | JP2001521904A (en) |
AU (1) | AU1289799A (en) |
CA (1) | CA2308136A1 (en) |
WO (1) | WO1999022754A1 (en) |
ZA (1) | ZA989744B (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009022006A1 (en) * | 2007-08-15 | 2009-02-19 | Novo Nordisk A/S | Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids |
WO2012171994A1 (en) | 2011-06-15 | 2012-12-20 | Novo Nordisk A/S | Multi substituted insulins |
US8933021B2 (en) | 2006-05-09 | 2015-01-13 | Novo Nordisk A/S | Insulin derivative |
US10040839B2 (en) | 2014-02-28 | 2018-08-07 | Novo Nordisk A/S | Insulin derivatives and the medical uses hereof |
US10137172B2 (en) | 2013-04-30 | 2018-11-27 | Novo Nordisk A/S | Administration regime |
US10335464B1 (en) | 2018-06-26 | 2019-07-02 | Novo Nordisk A/S | Device for titrating basal insulin |
US10596229B2 (en) | 2010-10-27 | 2020-03-24 | Novo Nordisk A/S | Method of treating diabetes mellitus by administration, at specifically defined intervals, of a derivative of a naturally occurring insulin or insulin analogue, the derivative having a prolonged profile of action |
US11167035B2 (en) | 2005-12-28 | 2021-11-09 | Novo Nordisk A/S | Insulin compositions and method of making a composition |
Families Citing this family (129)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20010041786A1 (en) * | 1995-06-07 | 2001-11-15 | Mark L. Brader | Stabilized acylated insulin formulations |
IL134901A0 (en) | 1997-10-24 | 2001-05-20 | Lilly Co Eli | Insoluble insulin compositions |
US6444641B1 (en) | 1997-10-24 | 2002-09-03 | Eli Lilly Company | Fatty acid-acylated insulin analogs |
ZA989744B (en) * | 1997-10-31 | 2000-04-26 | Lilly Co Eli | Method for administering acylated insulin. |
US6531448B1 (en) | 1997-12-23 | 2003-03-11 | Eli Lilly And Company | Insoluble compositions for controlling blood glucose |
US20060171899A1 (en) * | 1998-12-10 | 2006-08-03 | Akwete Adjei | Water-stabilized aerosol formulation system and method of making |
EP1055428B1 (en) * | 1999-05-25 | 2004-11-17 | USE Techno Corporation | Liquid composition to be vaporized for inhibiting increase in blood sugar lever and vaporizer for the same |
US7169889B1 (en) * | 1999-06-19 | 2007-01-30 | Biocon Limited | Insulin prodrugs hydrolyzable in vivo to yield peglylated insulin |
US9006175B2 (en) * | 1999-06-29 | 2015-04-14 | Mannkind Corporation | Potentiation of glucose elimination |
PT1808438E (en) * | 1999-06-29 | 2015-01-14 | Mannkind Corp | Purification and stabilization of peptide and proteins in pharmaceutical agents |
US6596261B1 (en) * | 2000-01-25 | 2003-07-22 | Aeropharm Technology Incorporated | Method of administering a medicinal aerosol formulation |
JP4711520B2 (en) * | 2000-03-21 | 2011-06-29 | 日本ケミカルリサーチ株式会社 | Bioactive peptide-containing powder |
JP2003528701A (en) * | 2000-04-03 | 2003-09-30 | バテル メモリアル インスティチュート | Dispensing device and liquid compound |
AU2001254621A1 (en) * | 2000-05-05 | 2001-11-20 | K.U. Leuven R And D | Critical illness neuropathy |
US20020120109A1 (en) * | 2000-07-07 | 2002-08-29 | Samokhin Gennady P. | Biologically active proteins |
JP4147234B2 (en) * | 2004-09-27 | 2008-09-10 | キヤノン株式会社 | Discharge liquid, discharge method, cartridge, and discharge device |
US20020106331A1 (en) * | 2000-12-08 | 2002-08-08 | Joan Rosell | Use of electrolytes (ions in solution) to suppress charging of inhalation aerosols |
WO2002051428A1 (en) * | 2000-12-25 | 2002-07-04 | Shiseido Company, Ltd. | Sympathetic-activating perfume composition |
US6867183B2 (en) * | 2001-02-15 | 2005-03-15 | Nobex Corporation | Pharmaceutical compositions of insulin drug-oligomer conjugates and methods of treating diseases therewith |
US7060675B2 (en) * | 2001-02-15 | 2006-06-13 | Nobex Corporation | Methods of treating diabetes mellitus |
JP2005501810A (en) | 2001-03-22 | 2005-01-20 | バテル メモリアル インスティチュート | Liquid form for electrohydrodynamic spraying containing polymer and suspended particles |
DE10114178A1 (en) | 2001-03-23 | 2002-10-10 | Aventis Pharma Gmbh | Zinc-free and low-zinc insulin preparations with improved stability |
IL158192A0 (en) * | 2001-05-04 | 2004-03-28 | Pfizer Prod Inc | Method of preventing type 2 diabetes with aerosolized insulin |
US6835802B2 (en) | 2001-06-04 | 2004-12-28 | Nobex Corporation | Methods of synthesizing substantially monodispersed mixtures of polymers having polyethylene glycol moieties |
US6828305B2 (en) * | 2001-06-04 | 2004-12-07 | Nobex Corporation | Mixtures of growth hormone drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same |
US7713932B2 (en) | 2001-06-04 | 2010-05-11 | Biocon Limited | Calcitonin drug-oligomer conjugates, and uses thereof |
US6828297B2 (en) * | 2001-06-04 | 2004-12-07 | Nobex Corporation | Mixtures of insulin drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same |
US6713452B2 (en) | 2001-06-04 | 2004-03-30 | Nobex Corporation | Mixtures of calcitonin drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same |
US20030035774A1 (en) * | 2001-07-18 | 2003-02-20 | Adjei Akwete L. | Salt/ion pair medicinal aerosol formulation |
US7196059B2 (en) * | 2001-09-07 | 2007-03-27 | Biocon Limited | Pharmaceutical compositions of insulin drug-oligomer conjugates and methods of treating diseases therewith |
US7030082B2 (en) * | 2001-09-07 | 2006-04-18 | Nobex Corporation | Pharmaceutical compositions of drug-oligomer conjugates and methods of treating disease therewith |
US7312192B2 (en) * | 2001-09-07 | 2007-12-25 | Biocon Limited | Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same |
US7166571B2 (en) * | 2001-09-07 | 2007-01-23 | Biocon Limited | Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same |
US6913903B2 (en) * | 2001-09-07 | 2005-07-05 | Nobex Corporation | Methods of synthesizing insulin polypeptide-oligomer conjugates, and proinsulin polypeptide-oligomer conjugates and methods of synthesizing same |
US6770625B2 (en) | 2001-09-07 | 2004-08-03 | Nobex Corporation | Pharmaceutical compositions of calcitonin drug-oligomer conjugates and methods of treating diseases therewith |
JP2005514188A (en) * | 2001-12-07 | 2005-05-19 | エイフェル テクノロジーズ リミテッド | Synthesis of small particles |
US20030199445A1 (en) * | 2002-02-07 | 2003-10-23 | Knudsen Lotte Bjerre | Use of GLP-1 compound for treatment of critically ill patients |
US20040005999A1 (en) * | 2002-03-07 | 2004-01-08 | Andreasen Kasper Huus | Polyamino acid-based particle insulin preparation |
WO2003080149A2 (en) | 2002-03-20 | 2003-10-02 | Mannkind Corporation | Inhalation apparatus |
AU2003226603A1 (en) * | 2002-04-19 | 2003-11-03 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Beta-agonist compounds comprising nitric oxide donor groups and reactive oxygen species scavenger groups and their use in the treatment of respiratory disorders |
US6985798B2 (en) | 2002-05-10 | 2006-01-10 | Oriel Therapeutics, Inc. | Dry powder dose filling systems and related methods |
US7677411B2 (en) * | 2002-05-10 | 2010-03-16 | Oriel Therapeutics, Inc. | Apparatus, systems and related methods for processing, dispensing and/or evaluatingl dry powders |
US6889690B2 (en) * | 2002-05-10 | 2005-05-10 | Oriel Therapeutics, Inc. | Dry powder inhalers, related blister devices, and associated methods of dispensing dry powder substances and fabricating blister packages |
DE10227232A1 (en) * | 2002-06-18 | 2004-01-15 | Aventis Pharma Deutschland Gmbh | Sour insulin preparations with improved stability |
JP2006507110A (en) * | 2002-06-27 | 2006-03-02 | オリエル・セラピューティクス,インコーポレイテッド | Apparatus, system and related methods for processing, dispensing and / or evaluating non-pharmaceutical dry powders |
WO2004037843A2 (en) * | 2002-10-25 | 2004-05-06 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Steroid compounds comprising superoxide dismutase mimic groups and nitric oxide donor groups, and their use in the preparation of medicaments |
ATE433994T1 (en) * | 2003-07-25 | 2009-07-15 | Conjuchem Biotechnologies Inc | INSULIN DERIVATIVES WITH LONG-LASTING EFFECTS AND METHOD FOR THE PRODUCTION THEREOF |
US7591801B2 (en) | 2004-02-26 | 2009-09-22 | Dexcom, Inc. | Integrated delivery device for continuous glucose sensor |
CA2531988C (en) | 2003-08-05 | 2016-06-28 | Novo Nordisk A/S | Novel insulin derivatives |
US7451761B2 (en) * | 2003-10-27 | 2008-11-18 | Oriel Therapeutics, Inc. | Dry powder inhalers, related blister package indexing and opening mechanisms, and associated methods of dispensing dry powder substances |
US7377277B2 (en) * | 2003-10-27 | 2008-05-27 | Oriel Therapeutics, Inc. | Blister packages with frames and associated methods of fabricating dry powder drug containment systems |
US20070027063A1 (en) * | 2004-01-12 | 2007-02-01 | Mannkind Corporation | Method of preserving the function of insulin-producing cells |
RU2393168C2 (en) | 2004-07-19 | 2010-06-27 | Биокон Лимитед | Insulin-oligomer conjugates, preparations and use thereof |
JP5078014B2 (en) | 2004-08-20 | 2012-11-21 | マンカインド コーポレイション | Catalytic reaction of diketopiperazine synthesis. |
HUE026797T2 (en) | 2004-08-23 | 2016-07-28 | Mannkind Corp | Diketopiperazine salts for drug delivery |
JP4147235B2 (en) * | 2004-09-27 | 2008-09-10 | キヤノン株式会社 | Discharge liquid, discharge method, droplet forming method, liquid discharge cartridge, and discharge apparatus |
EP2292653B1 (en) * | 2005-02-02 | 2014-05-21 | Novo Nordisk A/S | Novel insulin derivatives |
KR20120060245A (en) | 2005-09-14 | 2012-06-11 | 맨카인드 코포레이션 | Method of drug formulation based on increasing the affinity of crystalline microparticle surfaces for active agents |
EP2497484A3 (en) | 2006-02-22 | 2012-11-07 | MannKind Corporation | A method for improving the pharmaceutic properties of microparticles comprising diketopiperazine and an active agent |
WO2007098507A2 (en) | 2006-02-24 | 2007-08-30 | Rigel Pharmaceuticals, Inc. | Compositions and methods for inhibition of the jak pathway |
WO2007121318A2 (en) * | 2006-04-12 | 2007-10-25 | Emisphere Technologies, Inc. | Formulations for delivering insulin |
US20090306337A1 (en) | 2006-07-31 | 2009-12-10 | Novo Nordisk A/S | Pegylated, Extended Insulins |
RU2524150C2 (en) | 2006-09-22 | 2014-07-27 | Ново Нордиск А/С | Protease-resistant insulin analogues |
WO2008132224A2 (en) | 2007-04-30 | 2008-11-06 | Novo Nordisk A/S | Method for drying a protein composition, a dried protein composition and a pharmaceutical composition comprising the dried protein |
US9034818B2 (en) | 2007-06-13 | 2015-05-19 | Novo Nordisk A/S | Pharmaceutical formulations comprising an insulin derivative |
EP4159114B1 (en) | 2007-10-09 | 2024-04-10 | DexCom, Inc. | Integrated insulin delivery system with continuous glucose sensor |
MX2010003979A (en) * | 2007-10-16 | 2010-06-02 | Biocon Ltd | An orally administerable solid pharmaceutical composition and a process thereof. |
US8785396B2 (en) | 2007-10-24 | 2014-07-22 | Mannkind Corporation | Method and composition for treating migraines |
CA2702799A1 (en) | 2007-10-25 | 2009-04-30 | Dexcom, Inc. | Systems and methods for processing sensor data |
US9260502B2 (en) | 2008-03-14 | 2016-02-16 | Novo Nordisk A/S | Protease-stabilized insulin analogues |
RU2571857C2 (en) | 2008-03-18 | 2015-12-20 | Ново Нордиск А/С | Acylated insulin analogues stabilised with respect to proteases |
US20090271021A1 (en) * | 2008-04-28 | 2009-10-29 | Popp Shane M | Execution system for the monitoring and execution of insulin manufacture |
EP3281663B8 (en) | 2008-06-13 | 2022-09-21 | MannKind Corporation | Breath powered dry powder inhaler for drug delivery |
US8485180B2 (en) | 2008-06-13 | 2013-07-16 | Mannkind Corporation | Dry powder drug delivery system |
US9364619B2 (en) | 2008-06-20 | 2016-06-14 | Mannkind Corporation | Interactive apparatus and method for real-time profiling of inhalation efforts |
TWI494123B (en) | 2008-08-11 | 2015-08-01 | Mannkind Corp | Use of ultrarapid acting insulin |
KR101939557B1 (en) | 2008-10-17 | 2019-01-17 | 사노피-아벤티스 도이칠란트 게엠베하 | Combination of an insulin and a GLP-1 agonist |
AU2009309623B9 (en) | 2008-10-30 | 2014-10-02 | Novo Nordisk A/S | Treating diabetes melitus using insulin injections with less than daily injection frequency |
US8314106B2 (en) | 2008-12-29 | 2012-11-20 | Mannkind Corporation | Substituted diketopiperazine analogs for use as drug delivery agents |
AU2010206683B2 (en) | 2009-01-23 | 2016-05-05 | Rigel Pharmaceuticals, Inc. | Compositions and methods for inhibition of the JAK pathway |
WO2010088294A1 (en) | 2009-01-28 | 2010-08-05 | Smartcells, Inc. | Conjugate based systems for controlled drug delivery |
JP2012516340A (en) | 2009-01-28 | 2012-07-19 | スマートセルズ・インコーポレイテツド | Synthetic conjugates and uses thereof |
CA2754595C (en) | 2009-03-11 | 2017-06-27 | Mannkind Corporation | Apparatus, system and method for measuring resistance of an inhaler |
CA2754950A1 (en) | 2009-03-20 | 2010-09-23 | Smartcells, Inc. | Terminally-functionalized conjugates and uses thereof |
WO2010144789A2 (en) | 2009-06-12 | 2010-12-16 | Mannkind Corporation | Diketopiperazine microparticles with defined specific surface areas |
JP2012532177A (en) * | 2009-07-06 | 2012-12-13 | サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | Thermal and vibration stable insulin formulations |
WO2011051486A2 (en) | 2009-11-02 | 2011-05-05 | Novo Nordisk A/S | Pharmaceutical solution of non covalently bound albumin and acylated insulin |
CA2778698A1 (en) | 2009-11-03 | 2011-05-12 | Mannkind Corporation | An apparatus and method for simulating inhalation efforts |
RU2537239C2 (en) | 2009-11-13 | 2014-12-27 | Санофи-Авентис Дойчланд Гмбх | Pharmaceutical composition containing agonist glp-1, insulin and methionine |
AU2010317994B2 (en) | 2009-11-13 | 2014-03-06 | Sanofi-Aventis Deutschland Gmbh | Pharmaceutical composition comprising a GLP-1 agonist and methionine |
CA2782056C (en) | 2009-11-25 | 2021-03-09 | Arisgen Sa | Mucosal delivery of peptides |
EP2528601A1 (en) | 2010-01-26 | 2012-12-05 | Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. | Compositions and methods for prevention and treatment of pulmonary hypertension |
AU2011271097B2 (en) | 2010-06-21 | 2014-11-27 | Mannkind Corporation | Dry powder drug delivery system and methods |
CN103179978A (en) | 2010-08-30 | 2013-06-26 | 赛诺菲-安万特德国有限公司 | Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2 |
CN105667994B (en) | 2011-04-01 | 2018-04-06 | 曼金德公司 | Blister package for pharmaceutical kit |
US9821032B2 (en) | 2011-05-13 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin |
WO2012174472A1 (en) | 2011-06-17 | 2012-12-20 | Mannkind Corporation | High capacity diketopiperazine microparticles |
JP6367115B2 (en) | 2011-08-29 | 2018-08-01 | サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | Combination medicine used for blood glucose control in patients with type 2 diabetes |
AR087744A1 (en) | 2011-09-01 | 2014-04-16 | Sanofi Aventis Deutschland | PHARMACEUTICAL COMPOSITION FOR USE IN THE TREATMENT OF A NEURODEGENERATIVE DISEASE |
KR20140095483A (en) | 2011-10-24 | 2014-08-01 | 맨카인드 코포레이션 | Methods and compositions for treating pain |
JP6231548B2 (en) | 2012-03-23 | 2017-11-15 | マテオン セラピューティクス, インコーポレイテッド | Compositions and methods for inhibition of cathepsins |
MX2014012096A (en) | 2012-04-11 | 2014-11-21 | Novo Nordisk As | Insulin formulations. |
DK2872205T3 (en) | 2012-07-12 | 2017-02-27 | Mannkind Corp | DRY POWDER FORMAL ADMINISTRATION SYSTEM |
WO2014066856A1 (en) | 2012-10-26 | 2014-05-01 | Mannkind Corporation | Inhalable influenza vaccine compositions and methods |
EP2925345B1 (en) | 2012-12-03 | 2018-09-05 | Merck Sharp & Dohme Corp. | Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues |
KR102499439B1 (en) | 2013-03-15 | 2023-02-13 | 맨카인드 코포레이션 | Microcrystalline diketopiperazine compositions and methods |
EP2983697B1 (en) | 2013-04-03 | 2018-10-31 | Sanofi | Treatment of diabetes mellitus by long acting formulations of insulins |
WO2015010092A1 (en) | 2013-07-18 | 2015-01-22 | Mannkind Corporation | Heat-stable dry powder pharmaceutical compositions and methods |
JP2016530930A (en) | 2013-08-05 | 2016-10-06 | マンカインド コーポレイション | Ventilation device and method |
CA2924109A1 (en) | 2013-09-13 | 2015-03-19 | The California Institute For Biomedical Research | Modified therapeutic agents and compositions thereof |
KR20160065126A (en) | 2013-10-07 | 2016-06-08 | 노보 노르디스크 에이/에스 | Novel derivative of an insulin analogue |
KR102455171B1 (en) | 2013-12-18 | 2022-10-14 | 더 스크립스 리서치 인스티튜트 | Modified therapeutic agents, stapled peptide lipid conjugates, and compositions thereof |
WO2015148905A1 (en) | 2014-03-28 | 2015-10-01 | Mannkind Corporation | Use of ultrarapid acting insulin |
US10561806B2 (en) | 2014-10-02 | 2020-02-18 | Mannkind Corporation | Mouthpiece cover for an inhaler |
US20160158471A1 (en) * | 2014-12-05 | 2016-06-09 | Dance Biopharm, Inc. | Integration of glucose data to adjust inhaled insulin dose |
NZ733670A (en) | 2014-12-12 | 2021-12-24 | Sanofi Aventis Deutschland | Insulin glargine/lixisenatide fixed ratio formulation |
TWI748945B (en) | 2015-03-13 | 2021-12-11 | 德商賽諾菲阿凡提斯德意志有限公司 | Treatment type 2 diabetes mellitus patients |
TW201705975A (en) | 2015-03-18 | 2017-02-16 | 賽諾菲阿凡提斯德意志有限公司 | Treatment of type 2 diabetes mellitus patients |
HRP20221324T1 (en) | 2016-12-16 | 2022-12-23 | Novo Nordisk A/S | Insulin containing pharmaceutical compositions |
US11413352B2 (en) | 2017-12-18 | 2022-08-16 | Merck, Sharp & Dohme LLC | Conjugate based systems for controlled insulin delivery |
EP3727424A4 (en) | 2017-12-18 | 2021-10-27 | Merck Sharp & Dohme Corp. | Conjugate based systems for controlled insulin delivery |
WO2020092845A1 (en) | 2018-11-01 | 2020-05-07 | Rigel Pharmaceuticals, Inc. | Method and composition embodiments for treating acute myeloid leukemia |
US20200377518A1 (en) | 2019-05-29 | 2020-12-03 | Rigel Pharmaceuticals, Inc. | Method of preventing and treating thrombosis |
BR112022001418A2 (en) | 2019-08-08 | 2022-06-07 | Rigel Pharmaceuticals Inc | Compounds and method to treat cytokine release syndrome |
JP2022544276A (en) | 2019-08-14 | 2022-10-17 | ライジェル ファーマシューティカルズ, インコーポレイテッド | Methods of blocking or ameliorating cytokine release syndrome |
EP4126058A1 (en) | 2020-03-31 | 2023-02-08 | Protomer Technologies Inc. | Conjugates for selective responsiveness to vicinal diols |
IL302775A (en) | 2020-11-19 | 2023-07-01 | Protomer Tech Inc | Aromatic boron-containing compounds and insulin analogs |
US20230303555A1 (en) | 2022-03-23 | 2023-09-28 | Rigel Pharmaceuticals, Inc. | Pyrimid-2-yl-pyrazole compounds as irak inhibitors |
WO2023225534A1 (en) | 2022-05-18 | 2023-11-23 | Protomer Technologies Inc. | Aromatic boron-containing compounds and related insulin analogs |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5693609A (en) * | 1994-11-17 | 1997-12-02 | Eli Lilly And Company | Acylated insulin analogs |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01254699A (en) * | 1988-04-05 | 1989-10-11 | Kodama Kk | Insulin derivative and use thereof |
EP0705614B1 (en) * | 1989-04-28 | 2002-09-25 | Riker Laboratories, Inc. | Dry powder inhalation device |
US5320094A (en) * | 1992-01-10 | 1994-06-14 | The Johns Hopkins University | Method of administering insulin |
US6582728B1 (en) * | 1992-07-08 | 2003-06-24 | Inhale Therapeutic Systems, Inc. | Spray drying of macromolecules to produce inhaleable dry powders |
US5888477A (en) * | 1993-01-29 | 1999-03-30 | Aradigm Corporation | Use of monomeric insulin as a means for improving the bioavailability of inhaled insulin |
US5364838A (en) * | 1993-01-29 | 1994-11-15 | Miris Medical Corporation | Method of administration of insulin |
US6131567A (en) * | 1993-01-29 | 2000-10-17 | Aradigm Corporation | Method of use of monomeric insulin as a means for improving the reproducibility of inhaled insulin |
US5672581A (en) * | 1993-01-29 | 1997-09-30 | Aradigm Corporation | Method of administration of insulin |
US5743250A (en) * | 1993-01-29 | 1998-04-28 | Aradigm Corporation | Insulin delivery enhanced by coached breathing |
WO1995007931A1 (en) * | 1993-09-17 | 1995-03-23 | Novo Nordisk A/S | Acylated insulin |
AU689217B2 (en) * | 1994-03-07 | 1998-03-26 | Novartis Ag | Methods and compositions for pulmonary delivery of insulin |
US5869602A (en) * | 1995-03-17 | 1999-02-09 | Novo Nordisk A/S | Peptide derivatives |
US5700904A (en) * | 1995-06-07 | 1997-12-23 | Eli Lilly And Company | Preparation of an acylated protein powder |
US5898067A (en) * | 1997-02-07 | 1999-04-27 | Novo Nordisk A/S | Crystallization of proteins |
US5898028A (en) * | 1997-03-20 | 1999-04-27 | Novo Nordisk A/S | Method for producing powder formulation comprising an insulin |
CN1259142A (en) * | 1997-03-20 | 2000-07-05 | 诺沃挪第克公司 | Zinc free insulin crystals for use in pulmonary compositions |
ZA989744B (en) * | 1997-10-31 | 2000-04-26 | Lilly Co Eli | Method for administering acylated insulin. |
-
1998
- 1998-10-26 ZA ZA9809744A patent/ZA989744B/en unknown
- 1998-10-29 AU AU12897/99A patent/AU1289799A/en not_active Abandoned
- 1998-10-29 WO PCT/US1998/023040 patent/WO1999022754A1/en active Search and Examination
- 1998-10-29 JP JP2000518685A patent/JP2001521904A/en not_active Withdrawn
- 1998-10-29 EP EP98308850A patent/EP0919242A3/en not_active Withdrawn
- 1998-10-29 US US09/182,850 patent/US6051551A/en not_active Expired - Fee Related
- 1998-10-29 CA CA002308136A patent/CA2308136A1/en not_active Abandoned
-
2000
- 2000-03-10 US US09/523,090 patent/US6335316B1/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5693609A (en) * | 1994-11-17 | 1997-12-02 | Eli Lilly And Company | Acylated insulin analogs |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11167035B2 (en) | 2005-12-28 | 2021-11-09 | Novo Nordisk A/S | Insulin compositions and method of making a composition |
US8933021B2 (en) | 2006-05-09 | 2015-01-13 | Novo Nordisk A/S | Insulin derivative |
WO2009022006A1 (en) * | 2007-08-15 | 2009-02-19 | Novo Nordisk A/S | Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids |
US8962794B2 (en) | 2007-08-15 | 2015-02-24 | Novo Nordisk A/S | Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids |
US9035020B1 (en) | 2007-08-15 | 2015-05-19 | Novo Nordisk A/S | Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids |
US10596229B2 (en) | 2010-10-27 | 2020-03-24 | Novo Nordisk A/S | Method of treating diabetes mellitus by administration, at specifically defined intervals, of a derivative of a naturally occurring insulin or insulin analogue, the derivative having a prolonged profile of action |
WO2012171994A1 (en) | 2011-06-15 | 2012-12-20 | Novo Nordisk A/S | Multi substituted insulins |
US9260503B2 (en) | 2011-06-15 | 2016-02-16 | Novo Nordisk A/S | Multi-substituted insulins |
US10137172B2 (en) | 2013-04-30 | 2018-11-27 | Novo Nordisk A/S | Administration regime |
US10040839B2 (en) | 2014-02-28 | 2018-08-07 | Novo Nordisk A/S | Insulin derivatives and the medical uses hereof |
US10335464B1 (en) | 2018-06-26 | 2019-07-02 | Novo Nordisk A/S | Device for titrating basal insulin |
Also Published As
Publication number | Publication date |
---|---|
AU1289799A (en) | 1999-05-24 |
EP0919242A3 (en) | 2003-01-02 |
ZA989744B (en) | 2000-04-26 |
US6051551A (en) | 2000-04-18 |
CA2308136A1 (en) | 1999-05-14 |
EP0919242A2 (en) | 1999-06-02 |
US6335316B1 (en) | 2002-01-01 |
JP2001521904A (en) | 2001-11-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6051551A (en) | Method for administering acylated insulin | |
AU754707B2 (en) | Method for administering monomeric insulin analogs | |
EP0997151B1 (en) | Method for administering insulinotropic peptides | |
US6720407B1 (en) | Method for administering insulinotropic peptides | |
US20050203002A1 (en) | Sustained release compositions for delivery of pharmaceutical proteins | |
WO2000064940A1 (en) | Insulin crystals for pulmonary administration | |
WO2001093837A2 (en) | Protein powder for pulmonary delivery | |
EP1196445A1 (en) | Insulin crystals for pulmonary administration | |
US20040214747A1 (en) | Method for administering monomeric insulin | |
MXPA00006644A (en) | Method for administering monomeric insulin analogs | |
MXPA00006645A (en) | Method for administering aspb28-human insulin | |
EP1666054A1 (en) | Method for administering insulinotropic peptides | |
CZ20002417A3 (en) | Medicament intended for administration by inhalation | |
CZ20002418A3 (en) | Method of administering monomeric analog of insulin | |
MXPA01001905A (en) | Method for administering insulinotropic peptides |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AU AZ BA BB BG BR BY CA CN CU CZ EE GD GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LV MD MG MK MN MW MX NZ PL RO RU SD SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2308136 Country of ref document: CA Ref country code: JP Ref document number: 2000 518685 Kind code of ref document: A Format of ref document f/p: F Ref country code: CA Ref document number: 2308136 Kind code of ref document: A Format of ref document f/p: F |
|
NENP | Non-entry into the national phase |
Ref country code: KR |