WO1999005315A3 - Nucleic acid sequence analysis - Google Patents

Nucleic acid sequence analysis Download PDF

Info

Publication number
WO1999005315A3
WO1999005315A3 PCT/GB1998/002214 GB9802214W WO9905315A3 WO 1999005315 A3 WO1999005315 A3 WO 1999005315A3 GB 9802214 W GB9802214 W GB 9802214W WO 9905315 A3 WO9905315 A3 WO 9905315A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid sequence
sequence analysis
target polynucleotide
polynucleotide
Prior art date
Application number
PCT/GB1998/002214
Other languages
French (fr)
Other versions
WO1999005315A2 (en
Inventor
Daniel Henry Densham
Original Assignee
Medical Biosystems Ltd
Daniel Henry Densham
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26311958&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1999005315(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9715942.0A external-priority patent/GB9715942D0/en
Priority claimed from GBGB9727103.5A external-priority patent/GB9727103D0/en
Priority to CA2294962A priority Critical patent/CA2294962C/en
Priority to JP2000504282A priority patent/JP2001511358A/en
Priority to DE69808661T priority patent/DE69808661T3/en
Priority to DK98935212T priority patent/DK1017848T3/en
Priority to ES98935212T priority patent/ES2183394T5/en
Priority to AU84559/98A priority patent/AU735898B2/en
Priority to EP98935212A priority patent/EP1017848B2/en
Application filed by Medical Biosystems Ltd, Daniel Henry Densham filed Critical Medical Biosystems Ltd
Priority to BR9812270-3A priority patent/BR9812270A/en
Priority to NZ502557A priority patent/NZ502557A/en
Priority to AT98935212T priority patent/ATE225858T1/en
Priority to IL13341198A priority patent/IL133411A/en
Priority to US09/463,549 priority patent/US7008766B1/en
Publication of WO1999005315A2 publication Critical patent/WO1999005315A2/en
Publication of WO1999005315A3 publication Critical patent/WO1999005315A3/en
Priority to IS5360A priority patent/IS5360A/en
Priority to HK01100334A priority patent/HK1031130A1/en
Priority to US11/833,673 priority patent/US20080014592A1/en
Priority to US12/651,824 priority patent/US20100316999A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

The present invention relates to a method for determining the sequence of a polynucleotide, the method comprising the steps of: (i) reacting a target polynucleotide with a polymerase enzyme immobilised on a solid support, and the different nucleotides, under conditions sufficient for the polymerase reaction; and (ii) detecting the incorporation of a specific nucleotide complementary to the target polynucleotide, by measuring radiation.
PCT/GB1998/002214 1997-07-28 1998-07-24 Nucleic acid sequence analysis WO1999005315A2 (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
AT98935212T ATE225858T1 (en) 1997-07-28 1998-07-24 SEQUENCE ANALYSIS OF NUCLEIC ACIDS
IL13341198A IL133411A (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
US09/463,549 US7008766B1 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
NZ502557A NZ502557A (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
DE69808661T DE69808661T3 (en) 1997-07-28 1998-07-24 SEQUENCE ANALYSIS OF NUCLEIC ACIDS
DK98935212T DK1017848T3 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
ES98935212T ES2183394T5 (en) 1997-07-28 1998-07-24 Nucleic Acid Sequence Analysis
AU84559/98A AU735898B2 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
EP98935212A EP1017848B2 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
CA2294962A CA2294962C (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
BR9812270-3A BR9812270A (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
JP2000504282A JP2001511358A (en) 1997-07-28 1998-07-24 Analysis of nucleic acid sequence
IS5360A IS5360A (en) 1997-07-28 2000-01-25 Analysis of nucleic acid sequence
HK01100334A HK1031130A1 (en) 1997-07-28 2001-01-12 Nucleic acid sequence analysis
US11/833,673 US20080014592A1 (en) 1997-07-28 2007-08-03 Nucleic Acid Sequence Analysis
US12/651,824 US20100316999A1 (en) 1997-07-28 2010-01-04 Nucleic Acid Sequence Analysis

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9715942.0 1997-07-28
GBGB9715942.0A GB9715942D0 (en) 1997-07-28 1997-07-28
GB9727103.5 1997-12-22
GBGB9727103.5A GB9727103D0 (en) 1997-12-22 1997-12-22

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/463,549 A-371-Of-International US7008766B1 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis
US10/786,951 Continuation US20040241719A1 (en) 1997-07-28 2004-02-24 Nucleic acid sequence analysis

Publications (2)

Publication Number Publication Date
WO1999005315A2 WO1999005315A2 (en) 1999-02-04
WO1999005315A3 true WO1999005315A3 (en) 1999-04-22

Family

ID=26311958

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/002214 WO1999005315A2 (en) 1997-07-28 1998-07-24 Nucleic acid sequence analysis

Country Status (19)

Country Link
US (4) US7008766B1 (en)
EP (4) EP1229133B1 (en)
JP (1) JP2001511358A (en)
KR (1) KR100664331B1 (en)
CN (1) CN1152140C (en)
AT (1) ATE225858T1 (en)
AU (1) AU735898B2 (en)
BR (1) BR9812270A (en)
CA (1) CA2294962C (en)
DE (1) DE69808661T3 (en)
DK (1) DK1017848T3 (en)
ES (1) ES2183394T5 (en)
HK (1) HK1031130A1 (en)
IL (1) IL133411A (en)
IS (1) IS5360A (en)
NZ (1) NZ502557A (en)
PT (1) PT1017848E (en)
RU (1) RU2198221C2 (en)
WO (1) WO1999005315A2 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9175342B2 (en) 2007-10-19 2015-11-03 The Trustees Of Columbia University In The City Of New York Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis

Families Citing this family (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
IL131332A (en) 1997-02-12 2003-07-31 Eugene Y Chan Methods and products for analyzing polymers
DE69808661T3 (en) * 1997-07-28 2012-05-03 Gen-Probe Inc. SEQUENCE ANALYSIS OF NUCLEIC ACIDS
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AU2180200A (en) 1998-12-14 2000-07-03 Li-Cor Inc. A heterogeneous assay for pyrophosphate detection
DK1159453T3 (en) * 1999-03-10 2008-10-06 Asm Scient Inc Method of Direct Nucleic Acid Sequencing
AU2005201777B2 (en) * 1999-03-10 2007-08-02 Asm Scientific, Inc. A Method for Direct Nucleic Acid Sequencing
GB9907812D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
GB9907813D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
AU5059800A (en) * 1999-04-08 2000-11-14 Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts Nucleoside derivatives with photo-unstable protective groups
EP1681356B1 (en) * 1999-05-19 2011-10-19 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
WO2001016375A2 (en) * 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
AU3163101A (en) * 1999-12-21 2001-07-03 Vbc-Genomics Bioscience Research Gmbh Compound comprising a nucleic acid moiety and an organo-silane moiety
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AU2002231934A1 (en) * 2001-01-30 2002-08-12 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
GB0112238D0 (en) * 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
JP2004347621A (en) * 2003-04-24 2004-12-09 Dainippon Printing Co Ltd Transmission type screen
GB0316553D0 (en) * 2003-07-15 2003-08-20 Densham Daniel Method
EP1644524A1 (en) * 2003-07-15 2006-04-12 Daniel Henry Densham Measurement of a polynucleotide amplification reaction
GB0317343D0 (en) 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1704256A4 (en) 2004-01-13 2008-01-16 Us Genomics Inc Detection and quantification of analytes in solution using polymers
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
CA2693280C (en) 2004-04-09 2017-09-12 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
GB0413082D0 (en) 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
GB2423819B (en) 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
US7274458B2 (en) * 2005-03-07 2007-09-25 3M Innovative Properties Company Thermoplastic film having metallic nanoparticle coating
US7666494B2 (en) 2005-05-04 2010-02-23 3M Innovative Properties Company Microporous article having metallic nanoparticle coating
CN101218497B (en) * 2005-07-07 2012-04-25 索尼株式会社 Material information acquiring method and material information measuring device using evanescent light, and method and system for determination of base sequence
EP1902151A2 (en) 2005-07-14 2008-03-26 3M Innovative Properties Company Water-soluble polymeric substrate having metallic nanoparticle coating
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
SG166097A1 (en) * 2005-09-16 2010-11-29 Monsanto Technology Llc Methods for genetic control of insect infestations in plants and compositions thereof
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
US7871777B2 (en) 2005-12-12 2011-01-18 The United States Of America As Represented By The Department Of Health And Human Services Probe for nucleic acid sequencing and methods of use
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
EP4220138A1 (en) * 2006-09-01 2023-08-02 Pacific Biosciences of California, Inc. Substrates, systems and methods for analyzing materials
US20080076123A1 (en) * 2006-09-27 2008-03-27 Helicos Biosciences Corporation Polymerase variants for DNA sequencing
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
DE112007002932B4 (en) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable, reversible, fluorescent nucleotide terminators
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
DE102007022915A1 (en) * 2007-05-14 2008-11-20 Stiftung Caesar Center Of Advanced European Studies And Research Method for analyzing polymerization of analyte in fluid, particularly organic monomer, involves overflowing reaction medium before beginning of analyzing sensor surface
AU2008282862B2 (en) 2007-07-26 2014-07-31 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
EP2203566A4 (en) 2007-09-28 2011-02-09 Pacific Biosciences California Error-free amplification of dna for clonal sequencing
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
AU2009204461A1 (en) 2008-01-10 2009-07-16 Pacific Biosciences Of California, Inc. Methods and systems for analysis of fluorescent reactions with modulated excitation
WO2009102470A2 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
MX2010010600A (en) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Compositions and methods for nucleic acid sequencing.
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US8795961B2 (en) 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
WO2010033193A2 (en) * 2008-09-16 2010-03-25 Pacific Biosciences Of California, Inc. Substrates and optical systems and methods of use thereof
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8252910B2 (en) 2008-11-19 2012-08-28 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US8993230B2 (en) 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
WO2010068289A2 (en) 2008-12-11 2010-06-17 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
CA2760155A1 (en) 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US8518643B2 (en) 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
EP3460458B1 (en) 2010-02-19 2021-08-11 Pacific Biosciences of California, Inc. A method for nucleic acid sequencing
WO2011116120A2 (en) * 2010-03-16 2011-09-22 Life Technologies Corporation Method and apparatus for addressable flow cells in single molecule sequencing
US8318094B1 (en) 2010-06-18 2012-11-27 Pacific Biosciences Of California, Inc. Substrate analysis systems
WO2012021733A2 (en) 2010-08-12 2012-02-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
US8465922B2 (en) 2010-08-26 2013-06-18 Pacific Biosciences Of California, Inc. Methods and systems for monitoring reactions
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2012138973A2 (en) 2011-04-06 2012-10-11 The University Of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
WO2013019759A1 (en) * 2011-07-30 2013-02-07 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9175348B2 (en) 2012-04-24 2015-11-03 Pacific Biosciences Of California, Inc. Identification of 5-methyl-C in nucleic acid templates
US9593373B2 (en) 2013-03-15 2017-03-14 Illumina Cambridge Limited Modified nucleosides or nucleotides
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
WO2017190018A1 (en) 2016-04-29 2017-11-02 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP4039824A1 (en) 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Method and system for sequencing nucleic acids
WO2018125759A1 (en) 2016-12-30 2018-07-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
US9932631B1 (en) 2017-09-11 2018-04-03 Omniome, Inc. Genotyping by polymerase binding
WO2018136117A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Allele-specific capture of nucleic acids
CA3050695C (en) 2017-01-20 2024-02-20 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US11078534B2 (en) * 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
EP3899542A4 (en) 2018-12-21 2022-09-14 Illumina, Inc. Sensing systems
CA3182910A1 (en) * 2020-11-16 2022-05-19 Illumina Inc. Incorporation and imaging mixes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005303A1 (en) * 1988-11-10 1990-05-17 Pharmacia Ab Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems
WO1990013666A1 (en) * 1989-05-11 1990-11-15 Amersham International Plc Sequencing method
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993021340A1 (en) * 1992-04-22 1993-10-28 Medical Research Council Dna sequencing method
WO1994021822A1 (en) * 1993-03-19 1994-09-29 Sequenom, Inc. Dna sequencing by mass spectrometry via exonuclease degradation

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3728032A (en) * 1971-10-13 1973-04-17 H Noll Flow cell for spectroscopic analysis of density gradients
GB2102005B (en) * 1981-07-23 1984-11-21 Mta Koezponti Kemiai Kutato In Process for synthesis of polydeoxynucleotides
US5064754A (en) * 1984-12-14 1991-11-12 Mills Randell L Genomic sequencing method
US4863849A (en) † 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
DE68926504T2 (en) 1988-07-20 1996-09-12 David Segev METHOD FOR AMPLIFICATING AND DETECTING NUCLEIC ACID SEQUENCES
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
JPH06504997A (en) 1990-12-06 1994-06-09 アフィメトリックス，　インコーポレイテッド Synthesis of immobilized polymers on a very large scale
DE4104076C2 (en) * 1991-02-11 1994-03-31 Bruker Analytische Messtechnik Device for nuclear magnetic resonance spectrometric measurement of chemical and / or physical reaction processes
US6004744A (en) † 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
GB9119735D0 (en) * 1991-09-16 1991-10-30 Secr Defence Gene probe biosensor method
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
SE9201984D0 (en) 1992-06-29 1992-06-29 Pharmacia Biosensor Ab IMPROVEMENT IN OPTICAL ASSAYS
US5360714A (en) * 1992-08-28 1994-11-01 Fox Chase Cancer Center Hepadnavirus polymerase gene product having RNA-dependent DNA priming and reverse transcriptase activities and methods of measuring the activities thereof
WO1994010128A1 (en) * 1992-11-02 1994-05-11 Affymax Technologies N.V. Novel photoreactive protecting groups
WO1995006138A1 (en) 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5485277A (en) * 1994-07-26 1996-01-16 Physical Optics Corporation Surface plasmon resonance sensor and methods for the utilization thereof
US5801042A (en) * 1994-08-18 1998-09-01 The Trustees Of Columbia University In The City Of New York Unique associated Kaposi's sarcoma virus sequences and uses thereof
US5635608A (en) * 1994-11-08 1997-06-03 Molecular Probes, Inc. α-carboxy caged compounds
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
US6331692B1 (en) * 1996-10-12 2001-12-18 Volker Krause Diode laser, laser optics, device for laser treatment of a workpiece, process for a laser treatment of workpiece
WO1998039484A1 (en) 1997-03-05 1998-09-11 Scriptgen Pharmaceuticals, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
DE69808661T3 (en) * 1997-07-28 2012-05-03 Gen-Probe Inc. SEQUENCE ANALYSIS OF NUCLEIC ACIDS
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) * 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB9907813D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6908736B1 (en) * 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB0112238D0 (en) * 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
GB0413082D0 (en) * 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
WO2013113402A1 (en) * 2012-02-03 2013-08-08 Agilent Technologies, Inc. Micromachined flow cell with freestanding fluidic tube

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005303A1 (en) * 1988-11-10 1990-05-17 Pharmacia Ab Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems
WO1990013666A1 (en) * 1989-05-11 1990-11-15 Amersham International Plc Sequencing method
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993021340A1 (en) * 1992-04-22 1993-10-28 Medical Research Council Dna sequencing method
WO1994021822A1 (en) * 1993-03-19 1994-09-29 Sequenom, Inc. Dna sequencing by mass spectrometry via exonuclease degradation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SCHWARZ T: "DETECTION OF NUCLEIC ACID HYBRIDIZATION USING SURFACE PLASMON RESONANCE", TRENDS IN BIOTECHNOLOGY, vol. 9, no. 10, 1 October 1991 (1991-10-01), pages 339 - 340, XP000247400 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9410200B2 (en) 2001-12-04 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9388463B2 (en) 2001-12-04 2016-07-12 Illumina Cambridge Limited Labelled nucleotides
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9388464B2 (en) 2002-08-23 2016-07-12 Illumina Cambridge Limited Modified nucleotides
US9410199B2 (en) 2002-08-23 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US9175342B2 (en) 2007-10-19 2015-11-03 The Trustees Of Columbia University In The City Of New York Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators

Also Published As

Publication number Publication date
US20040241719A1 (en) 2004-12-02
US7008766B1 (en) 2006-03-07
IL133411A (en) 2003-09-17
US20100316999A1 (en) 2010-12-16
CA2294962C (en) 2012-09-18
ES2183394T5 (en) 2012-05-03
EP2267165A3 (en) 2012-10-10
EP1229133A2 (en) 2002-08-07
EP1017848A2 (en) 2000-07-12
EP2267165A2 (en) 2010-12-29
EP2267164A3 (en) 2012-08-15
CN1265158A (en) 2000-08-30
AU735898B2 (en) 2001-07-19
AU8455998A (en) 1999-02-16
HK1031130A1 (en) 2001-06-01
WO1999005315A2 (en) 1999-02-04
DE69808661D1 (en) 2002-11-14
BR9812270A (en) 2000-07-18
ATE225858T1 (en) 2002-10-15
EP1229133A3 (en) 2004-10-13
US20080014592A1 (en) 2008-01-17
DK1017848T3 (en) 2003-02-10
RU2198221C2 (en) 2003-02-10
IL133411A0 (en) 2001-04-30
KR100664331B1 (en) 2007-01-02
DE69808661T2 (en) 2003-07-31
EP2267164A2 (en) 2010-12-29
EP1229133B1 (en) 2016-03-09
EP1017848B1 (en) 2002-10-09
CN1152140C (en) 2004-06-02
JP2001511358A (en) 2001-08-14
DE69808661T3 (en) 2012-05-03
PT1017848E (en) 2003-02-28
EP2267165B1 (en) 2016-11-30
IS5360A (en) 2000-01-25
ES2183394T3 (en) 2003-03-16
NZ502557A (en) 2002-02-01
EP1017848B2 (en) 2012-02-22
CA2294962A1 (en) 1999-02-04
EP2267164B1 (en) 2016-09-14
KR20010022392A (en) 2001-03-15

Similar Documents

Publication Publication Date Title
WO1999005315A3 (en) Nucleic acid sequence analysis
EP0905258A3 (en) Method for detecting nucleic acid sequences based on the use of solid phase immobilised nucleotide probes (line probe assay)
WO2002083839A3 (en) P450 single nucleotide polymorphism biochip analysis
AU2709995A (en) Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
ES2006377A6 (en) Method for assays of nucleic acid, a reagent combination and a kit therefor
AU685144B2 (en) Chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
CA2172248A1 (en) Self-sustained sequence replication electrochemiluminescent nucleic acid assay
CA2039517A1 (en) Dna probe signal amplification
NZ506333A (en) An assay for detecting and quantifying the presence of particular nucleic acid (DNA or RNA) sequences
WO1996006190A3 (en) Coupled amplification and ligation method
EP0374665A3 (en) Assay of sequences using amplified genes
AU587188B2 (en) Nucleic acid hybridazation assay employing detectable anti- hybrid antibodies
WO2003062791A3 (en) Detection of single nucleotide polymorphisms using planar waveguides
CA2266749A1 (en) The use of modular oligonucleotides as probes or primers in nucleic acid based assay
WO2002020838A3 (en) Method and system for detecting nucleic acids
WO2000060114A3 (en) Polynucleotide sequencing using a helicase
NZ322938A (en) Method of detecting telomerase activity
WO1997007235A3 (en) All-in-one nucleic acid amplification assay
WO2000032824A3 (en) Length determination of nucleic acid repeat sequences by discontinuous primer extension
WO2000061800A8 (en) Method for the analysis of aflp® reaction mixtures using primer extension techniques
EP1243659A4 (en) Method for detecting target nucleotide sequences
NO20000538L (en) Enhancing the specificity of nucleic acid amplification by nucleic acid
EP0950718A4 (en) Method for detecting dna with probe pna
SE9500341D0 (en) Method for detecting DNA sequence variations
CA2446310A1 (en) Analytical method and kit

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 133411

Country of ref document: IL

Ref document number: 98807623.3

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1998935212

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 84559/98

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2294962

Country of ref document: CA

Ref document number: 2294962

Country of ref document: CA

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: PA/a/2000/000715

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 502557

Country of ref document: NZ

Ref document number: 09463549

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 1020007000971

Country of ref document: KR

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1998935212

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1020007000971

Country of ref document: KR

WWG Wipo information: grant in national office

Ref document number: 84559/98

Country of ref document: AU

WWG Wipo information: grant in national office

Ref document number: 1998935212

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 1020007000971

Country of ref document: KR