WO1998051698A8 - Synthesis of oligonucleotides - Google Patents

Synthesis of oligonucleotides

Info

Publication number
WO1998051698A8
WO1998051698A8 PCT/SE1998/000893 SE9800893W WO9851698A8 WO 1998051698 A8 WO1998051698 A8 WO 1998051698A8 SE 9800893 W SE9800893 W SE 9800893W WO 9851698 A8 WO9851698 A8 WO 9851698A8
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotide
free
solid support
oligonucleotides
synthesis
Prior art date
Application number
PCT/SE1998/000893
Other languages
French (fr)
Other versions
WO1998051698A1 (en
Inventor
Marek Kwiatkowski
Ulf Landegren
Mats Nilsson
Original Assignee
Marek Kwiatkowski
Ulf Landegren
Mats Nilsson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marek Kwiatkowski, Ulf Landegren, Mats Nilsson filed Critical Marek Kwiatkowski
Priority to EP98923266A priority Critical patent/EP0983288B1/en
Priority to JP54915198A priority patent/JP2001525821A/en
Priority to US09/423,742 priority patent/US6313284B1/en
Priority to DE69835207T priority patent/DE69835207D1/en
Priority to CA002289204A priority patent/CA2289204A1/en
Publication of WO1998051698A1 publication Critical patent/WO1998051698A1/en
Publication of WO1998051698A8 publication Critical patent/WO1998051698A8/en
Priority to US09/952,944 priority patent/US6790946B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

A method of preparing an immobilized oligonucleotide having a free 3'-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3'-end and having a free 5'-end; ii) binding said oligonucleotide in a second position remote from the 3'-end to the solid support; and iii) selectively releasing the 3'-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3'-end.
PCT/SE1998/000893 1997-05-14 1998-05-14 Synthesis of oligonucleotides WO1998051698A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP98923266A EP0983288B1 (en) 1997-05-14 1998-05-14 Synthesis of oligonucleotides
JP54915198A JP2001525821A (en) 1997-05-14 1998-05-14 Oligonucleotide synthesis
US09/423,742 US6313284B1 (en) 1997-05-14 1998-05-14 Solid phase synthesis
DE69835207T DE69835207D1 (en) 1997-05-14 1998-05-14 SYNTHESIS OF OLIGONUCLEOTIDES
CA002289204A CA2289204A1 (en) 1997-05-14 1998-05-14 Synthesis of oligonucleotides
US09/952,944 US6790946B2 (en) 1997-05-14 2001-09-12 Synthesis of oligonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9701783A SE9701783D0 (en) 1997-05-14 1997-05-14 In situ synthesis of oligonucleotides or inverse orientation
SE9701783-4 1997-05-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/952,944 Continuation US6790946B2 (en) 1997-05-14 2001-09-12 Synthesis of oligonucleotides

Publications (2)

Publication Number Publication Date
WO1998051698A1 WO1998051698A1 (en) 1998-11-19
WO1998051698A8 true WO1998051698A8 (en) 1999-06-10

Family

ID=20406926

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1998/000893 WO1998051698A1 (en) 1997-05-14 1998-05-14 Synthesis of oligonucleotides

Country Status (8)

Country Link
US (2) US6313284B1 (en)
EP (1) EP0983288B1 (en)
JP (1) JP2001525821A (en)
AT (1) ATE332911T1 (en)
CA (1) CA2289204A1 (en)
DE (1) DE69835207D1 (en)
SE (1) SE9701783D0 (en)
WO (1) WO1998051698A1 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9701783D0 (en) * 1997-05-14 1997-05-14 Marek Kwiatkowski In situ synthesis of oligonucleotides or inverse orientation
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
WO2001038580A2 (en) * 1999-11-26 2001-05-31 Curagen Corporation Nucleic acid probe arrays
WO2001092566A2 (en) * 2000-05-26 2001-12-06 Noxxon Pharma Ag Immobilized nucleic acids and uses thereof
US6932943B1 (en) 2001-01-26 2005-08-23 Third Wave Technologies Nucleic acid synthesizers
US7435390B2 (en) * 2001-01-26 2008-10-14 Third Wave Technologies, Inc. Nucleic acid synthesizers
JP2004533257A (en) 2001-06-15 2004-11-04 キアテック アクチボラゲット Amplifiable probe
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
EP1756307A1 (en) * 2004-05-20 2007-02-28 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
US20060068424A1 (en) * 2004-08-13 2006-03-30 Fuji Photo Film Co., Ltd. Biosensor
EP1788875A4 (en) * 2004-08-20 2010-12-15 Dharmacon Inc Novel polynucleotide synthesis labeling chemistry
US9249460B2 (en) 2011-09-09 2016-02-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
JP2013198425A (en) * 2012-03-24 2013-10-03 Kanagawa Univ Method for producing oligonucleotide
CN106460232B (en) * 2014-05-23 2019-12-24 生捷科技控股公司 Oligonucleotide probe inversion method for in situ synthesized probe array
US10695735B2 (en) 2015-08-18 2020-06-30 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
WO2017031278A1 (en) * 2015-08-18 2017-02-23 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
WO2018102660A1 (en) * 2016-12-02 2018-06-07 Centrillion Technologies, Inc. In situ probe inversion process for constructing probe arrays
US20210032776A1 (en) * 2018-03-21 2021-02-04 Centrillion Technology Holdings Corporation Method and system for fabricating dna sequencing arrays

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU598946B2 (en) * 1987-06-24 1990-07-05 Howard Florey Institute Of Experimental Physiology And Medicine Nucleoside derivatives
SE9003743D0 (en) 1990-11-26 1990-11-26 Pharmacia Ab METHOD AND MEANS FOR OLIGONUCLEOTIDE SYNTHESIS
US5550215A (en) * 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
SE9701783D0 (en) * 1997-05-14 1997-05-14 Marek Kwiatkowski In situ synthesis of oligonucleotides or inverse orientation

Also Published As

Publication number Publication date
CA2289204A1 (en) 1998-11-19
WO1998051698A1 (en) 1998-11-19
US6313284B1 (en) 2001-11-06
US6790946B2 (en) 2004-09-14
EP0983288B1 (en) 2006-07-12
EP0983288A1 (en) 2000-03-08
US20020051994A1 (en) 2002-05-02
JP2001525821A (en) 2001-12-11
SE9701783D0 (en) 1997-05-14
ATE332911T1 (en) 2006-08-15
DE69835207D1 (en) 2006-08-24

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