WO1998000435A3 - Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides - Google Patents

Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides Download PDF

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Publication number
WO1998000435A3
WO1998000435A3 PCT/US1997/011496 US9711496W WO9800435A3 WO 1998000435 A3 WO1998000435 A3 WO 1998000435A3 US 9711496 W US9711496 W US 9711496W WO 9800435 A3 WO9800435 A3 WO 9800435A3
Authority
WO
WIPO (PCT)
Prior art keywords
techniques involving
dna
oligonucleotide linker
linked oligonucleotides
solid phase
Prior art date
Application number
PCT/US1997/011496
Other languages
French (fr)
Other versions
WO1998000435A2 (en
WO1998000435A9 (en
Inventor
Gregory L Verdine
Changhee Min
Original Assignee
Harvard College
Gregory L Verdine
Changhee Min
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Gregory L Verdine, Changhee Min filed Critical Harvard College
Priority to EP97937951A priority Critical patent/EP0917533A2/en
Priority to JP10504429A priority patent/JP2000514799A/en
Priority to AU40389/97A priority patent/AU739391B2/en
Publication of WO1998000435A2 publication Critical patent/WO1998000435A2/en
Publication of WO1998000435A3 publication Critical patent/WO1998000435A3/en
Publication of WO1998000435A9 publication Critical patent/WO1998000435A9/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

A technique is provided for linking an oligonucleotide, such as a single strand of DNA, to another moiety or solid phase. An oligonucleotide is provided with a linking functionality that can be defined by six neighboring hystaminyal purines, which can be coordinated to a metal ion that also is coordinated by a chelating linked to a solid phase or other moiety. The invention finds particular use in resolution of one strand of DNA from its complement by providing the first strand with the linking functionality and passing the DNA across a metal affinity chromatography solid phase.
PCT/US1997/011496 1996-07-03 1997-06-30 Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides WO1998000435A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP97937951A EP0917533A2 (en) 1996-07-03 1997-06-30 Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides
JP10504429A JP2000514799A (en) 1996-07-03 1997-06-30 Techniques using oligonucleotide linkers and immobilized and linked oligonucleotides
AU40389/97A AU739391B2 (en) 1996-07-03 1997-06-30 Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2119596P 1996-07-03 1996-07-03
US60/021,195 1996-07-03

Publications (3)

Publication Number Publication Date
WO1998000435A2 WO1998000435A2 (en) 1998-01-08
WO1998000435A3 true WO1998000435A3 (en) 1998-05-07
WO1998000435A9 WO1998000435A9 (en) 1998-06-04

Family

ID=21802886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/011496 WO1998000435A2 (en) 1996-07-03 1997-06-30 Oligonucleotide linker and techniques involving immobilized and linked oligonucleotides

Country Status (5)

Country Link
EP (1) EP0917533A2 (en)
JP (1) JP2000514799A (en)
AU (1) AU739391B2 (en)
CA (1) CA2259493A1 (en)
WO (1) WO1998000435A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPP856399A0 (en) * 1999-02-08 1999-03-04 Australian Membrane And Biotechnology Research Institute Improved compounds for protein binding
EP1212458A4 (en) * 1999-07-30 2005-01-05 Surromed Inc Instruments, methods and reagents for surface plasmon resonance
US7598371B2 (en) 2000-11-06 2009-10-06 University Of Houston Nucleic acid separation using immobilized metal affinity chromatography
EP1534755B1 (en) 2002-05-10 2011-10-12 Bio-Layer Pty Limited Generation of surface coating diversity
SE0203521D0 (en) * 2002-11-28 2002-11-28 Amersham Biosciences Ab Isolation of antisense oligonucleotides
US7893222B2 (en) * 2002-12-20 2011-02-22 University Of Houston Introduction of structural affinity handles as a tool in selective nucleic acid separations
US7371852B2 (en) * 2003-01-22 2008-05-13 Serenex, Inc. Alkyl-linked nucleotide compositions
US20070105116A1 (en) * 2003-09-02 2007-05-10 Japan Science And Technology Agency Metal complex type nucleic acid
WO2006002472A1 (en) 2004-07-02 2006-01-12 Bio-Layer Pty Ltd Use of metal complexes
WO2008134472A1 (en) * 2007-04-25 2008-11-06 3M Innovative Properties Company Compositions, methods, and devices for isolating biological materials

Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0212951A2 (en) * 1985-08-15 1987-03-04 Amoco Corporation Labelled nucleic acids
EP0285057A2 (en) * 1982-06-23 1988-10-05 Enzo Biochem, Inc. Modified labeled nucleotides and polynucleotides and methods of preparing, utilizing and detecting same
EP0340675A2 (en) * 1988-05-02 1989-11-08 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
WO1990000622A1 (en) * 1988-07-08 1990-01-25 Wallac Oy Oligonucleotide hybridization probes and means for the synthesis of the most preferred probes
US4952685A (en) * 1984-01-30 1990-08-28 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
EP0497585A2 (en) * 1991-01-30 1992-08-05 Leslie Deriemer Anderson Method of immobilizing and crosslinking proteins and other molecules and uses thereof
WO1992014841A1 (en) * 1991-02-14 1992-09-03 Baxter Diagnostics Inc. Novel lanthanide chelate-conjugated oligonucleotides
EP0519250A2 (en) * 1991-06-10 1992-12-23 Miles Inc. Microparticle-labeled binding assay analyzed by microwave spectroscopy
US5175269A (en) * 1984-01-30 1992-12-29 Enzo Diagnostics, Inc. Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group
US5238808A (en) * 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
US5250188A (en) * 1989-09-01 1993-10-05 Brigham Young University Process of removing and concentrating desired molecules from solutions
US5256535A (en) * 1986-10-17 1993-10-26 Jyrki Ylikoski Hybridization assay and means to be used in the assay
US5294369A (en) * 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
GB2284208A (en) * 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
US5453356A (en) * 1984-10-31 1995-09-26 Igen, Inc. Luminescent metal chelate labels and means for detection
WO1996002274A1 (en) * 1994-07-14 1996-02-01 Schering Aktiengesellschaft Conjugates made of metal complexes and oligonucleotides
WO1996007668A1 (en) * 1994-09-02 1996-03-14 Novartis Ag Functional terpyridine metal complexes, methods for their preparation and oligonucleotide conjugates with terpyridine metal complexes
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers

Patent Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0285057A2 (en) * 1982-06-23 1988-10-05 Enzo Biochem, Inc. Modified labeled nucleotides and polynucleotides and methods of preparing, utilizing and detecting same
US5175269A (en) * 1984-01-30 1992-12-29 Enzo Diagnostics, Inc. Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group
US4952685A (en) * 1984-01-30 1990-08-28 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5238808A (en) * 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
US5453356A (en) * 1984-10-31 1995-09-26 Igen, Inc. Luminescent metal chelate labels and means for detection
EP0212951A2 (en) * 1985-08-15 1987-03-04 Amoco Corporation Labelled nucleic acids
US5256535A (en) * 1986-10-17 1993-10-26 Jyrki Ylikoski Hybridization assay and means to be used in the assay
EP0340675A2 (en) * 1988-05-02 1989-11-08 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
WO1990000622A1 (en) * 1988-07-08 1990-01-25 Wallac Oy Oligonucleotide hybridization probes and means for the synthesis of the most preferred probes
US5250188A (en) * 1989-09-01 1993-10-05 Brigham Young University Process of removing and concentrating desired molecules from solutions
US5294369A (en) * 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
EP0497585A2 (en) * 1991-01-30 1992-08-05 Leslie Deriemer Anderson Method of immobilizing and crosslinking proteins and other molecules and uses thereof
WO1992014841A1 (en) * 1991-02-14 1992-09-03 Baxter Diagnostics Inc. Novel lanthanide chelate-conjugated oligonucleotides
EP0519250A2 (en) * 1991-06-10 1992-12-23 Miles Inc. Microparticle-labeled binding assay analyzed by microwave spectroscopy
GB2284208A (en) * 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
WO1996002274A1 (en) * 1994-07-14 1996-02-01 Schering Aktiengesellschaft Conjugates made of metal complexes and oligonucleotides
WO1996007668A1 (en) * 1994-09-02 1996-03-14 Novartis Ag Functional terpyridine metal complexes, methods for their preparation and oligonucleotide conjugates with terpyridine metal complexes
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
C.MIN ET AL.: "Immobilized Metal Affinity Chromatography of DNA", NUCLEIC ACIDS RESEARCH., vol. 24, no. 19, 1 October 1996 (1996-10-01), OXFORD GB, pages 3806 - 10, XP002057408 *
C.MIN ET AL.: "Template Directed Interference of Footprinting of Protein-Adenine Contacts", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 118, no. 26, 3 July 1996 (1996-07-03), US, pages 6116 - 6120, XP002057407 *
PORATH J: "IMAC-IMMOBILIZED METAL ION AFFINITY BASED CHROMATOGRAPHY", TRAC, TRENDS IN ANALYTICAL CHEMISTRY, AMSTERDAM, NL, vol. 7, no. 7, August 1988 (1988-08-01), pages 254 - 259, XP000001799 *

Also Published As

Publication number Publication date
JP2000514799A (en) 2000-11-07
WO1998000435A2 (en) 1998-01-08
AU739391B2 (en) 2001-10-11
AU4038997A (en) 1998-01-21
EP0917533A2 (en) 1999-05-26
CA2259493A1 (en) 1998-01-08

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