WO1997037040A3 - Isolation and/or amplification of hepatitis c virus (hcv) nucleic acids from samples suspected to contain hcv - Google Patents

Isolation and/or amplification of hepatitis c virus (hcv) nucleic acids from samples suspected to contain hcv Download PDF

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Publication number
WO1997037040A3
WO1997037040A3 PCT/NL1997/000167 NL9700167W WO9737040A3 WO 1997037040 A3 WO1997037040 A3 WO 1997037040A3 NL 9700167 W NL9700167 W NL 9700167W WO 9737040 A3 WO9737040 A3 WO 9737040A3
Authority
WO
WIPO (PCT)
Prior art keywords
hcv
nucleic acid
amplification
single stranded
methods
Prior art date
Application number
PCT/NL1997/000167
Other languages
French (fr)
Other versions
WO1997037040A2 (en
Inventor
Jaap Goudsmit
Marcellinus Gualbertus Hu Beld
Cornelis Johannes Andreas Sol
Willem Rene Boom
Original Assignee
Amsterdam Support Diagnostics
Jaap Goudsmit
Marcellinus Gualbertus Hu Beld
Cornelis Johannes Andreas Sol
Willem Rene Boom
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amsterdam Support Diagnostics, Jaap Goudsmit, Marcellinus Gualbertus Hu Beld, Cornelis Johannes Andreas Sol, Willem Rene Boom filed Critical Amsterdam Support Diagnostics
Priority to AU21809/97A priority Critical patent/AU2180997A/en
Publication of WO1997037040A2 publication Critical patent/WO1997037040A2/en
Publication of WO1997037040A3 publication Critical patent/WO1997037040A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D

Abstract

The invention relates to methods for easily separating single stranded (HCV) nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at least one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such as chelating agents, pH and the like it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded HCV material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficient separations the process may be repeated. Following the separation of the two kinds of nucleic acid, either kind may be amplified. Methods of amplification are provided which do not need sequence data of the material to be amplified. In these methods a primer will be provided with an amplification motif and a random hybridization motif.
PCT/NL1997/000167 1996-04-03 1997-04-03 Isolation and/or amplification of hepatitis c virus (hcv) nucleic acids from samples suspected to contain hcv WO1997037040A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU21809/97A AU2180997A (en) 1996-04-03 1997-04-03 Isolation and/or amplification of hepatitis c virus (hcv) nucleic acids from samples suspected to contain hcv

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1002781 1996-04-03
NL1002781A NL1002781C1 (en) 1996-04-03 1996-04-03 Isolation and / or amplification of hepatitis C virus (HCV) nucleic acids from samples suspected of containing HCV.

Publications (2)

Publication Number Publication Date
WO1997037040A2 WO1997037040A2 (en) 1997-10-09
WO1997037040A3 true WO1997037040A3 (en) 1997-11-27

Family

ID=19762613

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL1997/000167 WO1997037040A2 (en) 1996-04-03 1997-04-03 Isolation and/or amplification of hepatitis c virus (hcv) nucleic acids from samples suspected to contain hcv

Country Status (3)

Country Link
AU (1) AU2180997A (en)
NL (1) NL1002781C1 (en)
WO (1) WO1997037040A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006031764B4 (en) * 2006-07-06 2009-10-01 Aj Innuscreen Gmbh A method for the parallel isolation of double- and single-stranded nucleic acids and for the selective removal of double-stranded nucleic acids from a mixture of double-stranded and single-stranded nucleic acids
EP2663649A4 (en) * 2011-01-10 2014-08-27 American University In Cairo Direct detection of unamplified hepatitis c virus rna using unmodified gold nanoparticles

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0389063A2 (en) * 1989-03-23 1990-09-26 Akzo Nobel N.V. Process for isolating nucleic acid
EP0488243A1 (en) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Method of extracting virus genome from sample derived from living body infected with the virus and detecting the genome
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
WO1995004140A1 (en) * 1993-07-28 1995-02-09 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
WO1995006652A1 (en) * 1993-08-30 1995-03-09 Promega Corporation Nucleic acid purification compositions and methods
GB2282138A (en) * 1993-09-28 1995-03-29 Tosoh Corp Method of extracting nucleic acids and detecting specified nucleic acid sequences
WO1995021849A1 (en) * 1994-02-11 1995-08-17 Qiagen Gmbh Process for separating double-stranded/single-stranded nucleic acid structures
WO1995034569A1 (en) * 1994-06-14 1995-12-21 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
WO1996003528A2 (en) * 1994-07-27 1996-02-08 Cambridge University Technical Services Limited Oligonucleotides and their use

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0389063A2 (en) * 1989-03-23 1990-09-26 Akzo Nobel N.V. Process for isolating nucleic acid
EP0488243A1 (en) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Method of extracting virus genome from sample derived from living body infected with the virus and detecting the genome
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
WO1995004140A1 (en) * 1993-07-28 1995-02-09 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
WO1995006652A1 (en) * 1993-08-30 1995-03-09 Promega Corporation Nucleic acid purification compositions and methods
GB2282138A (en) * 1993-09-28 1995-03-29 Tosoh Corp Method of extracting nucleic acids and detecting specified nucleic acid sequences
WO1995021849A1 (en) * 1994-02-11 1995-08-17 Qiagen Gmbh Process for separating double-stranded/single-stranded nucleic acid structures
WO1995034569A1 (en) * 1994-06-14 1995-12-21 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
WO1996003528A2 (en) * 1994-07-27 1996-02-08 Cambridge University Technical Services Limited Oligonucleotides and their use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BOOM R ET AL: "RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC ACIDS", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 28, no. 3, March 1990 (1990-03-01), pages 495 - 503, XP000608490 *

Also Published As

Publication number Publication date
WO1997037040A2 (en) 1997-10-09
NL1002781C1 (en) 1997-10-06
AU2180997A (en) 1997-10-22

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