WO1996010196A3 - Method and apparatus for automatic focusing of biomedical specimens - Google Patents

Method and apparatus for automatic focusing of biomedical specimens Download PDF

Info

Publication number
WO1996010196A3
WO1996010196A3 PCT/US1995/010133 US9510133W WO9610196A3 WO 1996010196 A3 WO1996010196 A3 WO 1996010196A3 US 9510133 W US9510133 W US 9510133W WO 9610196 A3 WO9610196 A3 WO 9610196A3
Authority
WO
WIPO (PCT)
Prior art keywords
images
automatic focusing
coverslip
focus measure
focus
Prior art date
Application number
PCT/US1995/010133
Other languages
French (fr)
Other versions
WO1996010196A2 (en
Inventor
Keith L Frost
Jon W Hayenga
James A Stephanick
Robert C Schmidt
Original Assignee
Neopath Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neopath Inc filed Critical Neopath Inc
Priority to CA002200463A priority Critical patent/CA2200463C/en
Priority to AU32171/95A priority patent/AU709136B2/en
Priority to JP8511734A priority patent/JPH10506206A/en
Priority to EP95928372A priority patent/EP0784805A2/en
Publication of WO1996010196A2 publication Critical patent/WO1996010196A2/en
Publication of WO1996010196A3 publication Critical patent/WO1996010196A3/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
    • G02B7/28—Systems for automatic generation of focusing signals

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Eye Examination Apparatus (AREA)
  • Automatic Focus Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

A method for automatically focusing on a slide includes the steps of locating a coverslip (401-411), acquiring images from predetermined focal depths in the slide (412), and starting at an initial focal depth proximate the surface of the coverslip (202). A set of predetermined characteristics within each of the images are measured (511) to generate at least one image measurement for each of the plurality of images. A focus measure is computed (511) for each of the images, where each focus measure is a function of at least one image measurement. A best focus location is determined (512) relative to a focal depth where an acquired image has a highest focus measure.
PCT/US1995/010133 1994-09-20 1995-08-08 Method and apparatus for automatic focusing of biomedical specimens WO1996010196A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002200463A CA2200463C (en) 1994-09-20 1995-08-08 Method and apparatus for automatic focusing of biomedical specimens
AU32171/95A AU709136B2 (en) 1994-09-20 1995-08-08 Automatic focusing of biomedical specimens apparatus
JP8511734A JPH10506206A (en) 1994-09-20 1995-08-08 Automatic focusing device for medical and biological specimens
EP95928372A EP0784805A2 (en) 1994-09-20 1995-08-08 Automatic focusing of biomedical specimens apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/309,405 1994-09-20
US08/309,405 US5647025A (en) 1994-09-20 1994-09-20 Automatic focusing of biomedical specimens apparatus

Publications (2)

Publication Number Publication Date
WO1996010196A2 WO1996010196A2 (en) 1996-04-04
WO1996010196A3 true WO1996010196A3 (en) 1996-07-04

Family

ID=23198101

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/010133 WO1996010196A2 (en) 1994-09-20 1995-08-08 Method and apparatus for automatic focusing of biomedical specimens

Country Status (6)

Country Link
US (1) US5647025A (en)
EP (1) EP0784805A2 (en)
JP (1) JPH10506206A (en)
AU (1) AU709136B2 (en)
CA (1) CA2200463C (en)
WO (1) WO1996010196A2 (en)

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Also Published As

Publication number Publication date
CA2200463C (en) 2002-04-16
AU709136B2 (en) 1999-08-19
EP0784805A2 (en) 1997-07-23
CA2200463A1 (en) 1996-04-04
US5647025A (en) 1997-07-08
JPH10506206A (en) 1998-06-16
WO1996010196A2 (en) 1996-04-04
AU3217195A (en) 1996-04-19

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